##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW225_GTGGCC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9287907	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72116064469638	34.0	31.0	34.0	30.0	34.0
2	32.03499044510243	34.0	31.0	34.0	30.0	34.0
3	32.42939361903602	34.0	31.0	34.0	30.0	34.0
4	36.06884963426098	37.0	35.0	37.0	35.0	37.0
5	36.03817243217444	37.0	35.0	37.0	35.0	37.0
6	36.051785402244015	37.0	35.0	37.0	35.0	37.0
7	36.049808638264786	37.0	35.0	37.0	35.0	37.0
8	36.04726780748343	37.0	35.0	37.0	35.0	37.0
9	37.55369126758052	39.0	37.0	39.0	35.0	39.0
10	37.67314627504345	39.0	37.0	39.0	35.0	39.0
11	37.71689746678127	39.0	37.0	39.0	35.0	39.0
12	37.68820015101357	39.0	37.0	39.0	35.0	39.0
13	37.6641388635782	39.0	37.0	39.0	35.0	39.0
14	39.115120554071005	40.0	39.0	41.0	36.0	41.0
15	39.06323060728321	40.0	38.0	41.0	36.0	41.0
16	39.077202969409576	40.0	38.0	41.0	36.0	41.0
17	39.046324430251076	40.0	38.0	41.0	36.0	41.0
18	39.00117927537388	40.0	38.0	41.0	36.0	41.0
19	39.05356179815323	40.0	39.0	41.0	36.0	41.0
20	39.021134147876374	40.0	39.0	41.0	36.0	41.0
21	38.966429681089615	40.0	38.0	41.0	36.0	41.0
22	38.89268615631057	40.0	38.0	41.0	35.0	41.0
23	38.834897140981276	40.0	38.0	41.0	35.0	41.0
24	38.75702125355045	40.0	38.0	41.0	35.0	41.0
25	38.729958321072765	40.0	38.0	41.0	35.0	41.0
26	38.62682076812354	40.0	38.0	41.0	34.0	41.0
27	38.5233155327675	40.0	38.0	41.0	34.0	41.0
28	38.39575719265923	40.0	38.0	41.0	34.0	41.0
29	38.114569837962414	40.0	38.0	41.0	34.0	41.0
30	38.066820221175774	40.0	38.0	41.0	33.0	41.0
31	38.34039595788373	40.0	38.0	41.0	34.0	41.0
32	38.318945484703924	40.0	38.0	41.0	34.0	41.0
33	38.353770122805926	40.0	38.0	41.0	34.0	41.0
34	38.47405082759765	40.0	38.0	41.0	34.0	41.0
35	38.468909841582175	40.0	38.0	41.0	34.0	41.0
36	38.49922065326451	40.0	38.0	41.0	34.0	41.0
37	38.453627604152366	40.0	38.0	41.0	34.0	41.0
38	38.40151004957306	40.0	38.0	41.0	34.0	41.0
39	38.34653663091157	40.0	38.0	41.0	34.0	41.0
40	38.29597012545453	40.0	38.0	41.0	34.0	41.0
41	38.30832963766756	40.0	38.0	41.0	34.0	41.0
42	38.27375177206232	40.0	38.0	41.0	34.0	41.0
43	38.231967977284874	40.0	38.0	41.0	33.0	41.0
44	38.169713047299034	40.0	38.0	41.0	33.0	41.0
45	38.02055027036769	40.0	38.0	41.0	33.0	41.0
46	37.92175826049938	40.0	37.0	41.0	33.0	41.0
47	37.844162845299806	40.0	37.0	41.0	33.0	41.0
48	37.8317914897296	40.0	37.0	41.0	33.0	41.0
49	37.7710851325277	40.0	37.0	41.0	33.0	41.0
50	37.69361385724469	40.0	37.0	41.0	33.0	41.0
51	36.67664200341368	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	3.0
13	3.0
14	9.0
15	30.0
16	86.0
17	176.0
18	395.0
19	862.0
20	1766.0
21	2825.0
22	4784.0
23	8047.0
24	13201.0
25	20334.0
26	29620.0
27	41544.0
28	57846.0
29	77219.0
30	101346.0
31	130998.0
32	168756.0
33	218910.0
34	289883.0
35	402419.0
36	595968.0
37	981263.0
38	2044257.0
39	4094336.0
40	1015.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.69505634481601	26.61542021252187	32.043597510991155	22.645925931670963
2	19.099230472152865	31.28166820863655	29.732739456904316	19.88636186230627
3	18.565991240007033	29.585642922565867	32.60621580297908	19.24215003444802
4	18.345349495855203	28.485610374867015	31.10035447168022	22.068685657597563
5	21.459689465021558	29.4492720480513	31.36815431076129	17.722884176165845
6	21.341675794126708	30.07561337554306	29.493943037974002	19.088767792356233
7	19.308763535207664	32.43441175713753	30.389731507862862	17.867093199791945
8	18.43526211018263	32.877988550057616	30.158753742904622	18.52799559685514
9	20.95865814216972	29.541568012313657	30.572263974877416	18.927509870639206
10	18.442260457603634	32.38640309382943	29.950192223070278	19.22114422549666
11	21.104194949411102	29.53545938821308	30.023211903392227	19.33713375898359
12	18.459659425961092	29.68160641574038	29.869948094872186	21.988786063426346
13	18.4283929630217	32.606689537266035	29.82667677443368	19.13824072527858
14	18.41411633428285	30.051862061064995	29.88957576771602	21.644445836936136
15	18.278714461718877	32.73990577209698	30.01180998044016	18.96956978574398
16	18.305760382828986	29.988866167587595	30.05942027627968	21.645953173303738
17	21.09813557544618	29.925162894628777	30.072278939946205	18.90442258997884
18	18.48136506965455	29.743988607982402	32.71257991709004	19.062066405273008
19	18.501085557429565	29.72342914483484	29.964787348639703	21.81069794909589
20	18.57774846367432	29.70140635559766	32.581549319992114	19.1392958607359
21	21.32551846570415	29.631187661478815	29.8257805356736	19.217513337143433
22	18.621201333305056	32.40770219566389	29.797197363407452	19.17389910762361
23	18.61650463223415	32.433585083823814	29.84280713012516	19.107103153816876
24	18.615076695400344	29.75568981067129	29.834583953187682	21.79464954074069
25	18.55253287958202	29.75374322761845	32.540625137611734	19.153098755187795
26	18.619792900861434	29.737810715489005	29.888014462644346	21.754381921005216
27	18.67357632377951	29.761508047205098	29.78734656026814	21.777569068747248
28	18.656319430918554	32.45506004347054	29.7287788087141	19.159841716896803
29	21.425509876832134	29.76109680942351	29.724660009881166	19.08873330386319
30	18.784939349851086	29.683845713390006	32.333954764614724	19.197260172144183
31	18.71421278600833	29.678461670821754	29.711798553915784	21.895526989254133
32	18.775313236739592	32.36454013655718	29.66250382699122	19.197642799712007
33	18.798106526057346	29.708307174799746	29.689448258770607	21.804138040372298
34	21.38603116323266	29.745169457723225	29.718980419386437	19.149818959657683
35	18.799709442306682	29.83268597458288	32.337432410664064	19.030172172446377
36	21.381211114576207	29.784587028399823	29.73638676340605	19.097815093617925
37	21.35520794132669	29.82370390890446	29.723278403755764	19.097809746013084
38	18.800061519731226	29.834075512023595	29.686889124254996	21.678973843990185
39	18.81398911256225	29.827525823956414	29.66722322952058	21.69126183396076
40	18.74933291429671	32.41471615563908	29.701493485743185	19.13445744432103
41	18.77419747389438	29.779303638283473	32.328692034695855	19.117806853126293
42	18.82403226138207	29.753160194148098	29.694242917300105	21.728564627169728
43	18.761471901192063	29.704324871749478	32.350610592681335	19.183592634377128
44	18.812775908179134	29.710031783416195	29.76479884358157	21.712393464823094
45	21.366534544043432	29.808022368533827	29.779951874230402	19.045491213192335
46	18.710712213178425	29.867291235939756	32.31792718595297	19.10406936492885
47	18.805407244153095	32.43296589626815	29.815661935358545	18.94596492422021
48	18.834867672655708	29.8111046166291	32.31998954182602	19.03403816888917
49	21.41543936524161	29.76943456533516	29.735998684532305	19.079127384890924
50	18.8885780331533	29.75628120627245	29.681180470707375	21.67396028986687
51	18.874853952306342	29.691299595643066	29.67051640310779	21.7633300489428
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.340982276486976
2	38.985592334459135
3	37.80814127445505
4	40.414035153452765
5	39.18257364118741
6	40.43044358648294
7	37.175856734999606
8	36.96325770703777
9	39.88616801280892
10	37.663404683100296
11	40.44132870839469
12	40.44844548938744
13	37.566633688300286
14	40.05856217121899
15	37.24828424746286
16	39.951713556132724
17	40.002558165425015
18	37.54343147492756
19	40.31178350652546
20	37.717044324410224
21	40.54303180284759
22	37.795100440928664
23	37.72360778605102
24	40.40972623614103
25	37.705631634769816
26	40.374174821866646
27	40.451145392526755
28	37.81616114781536
29	40.51424318069532
30	37.98219952199528
31	40.60973977526246
32	37.9729560364516
33	40.60224456642964
34	40.53585012289034
35	37.829881614753056
36	40.47902620819413
37	40.45301768733977
38	40.47903536372141
39	40.50525094652301
40	37.88379035861774
41	37.89200432702067
42	40.5525968885518
43	37.945064535569195
44	40.52516937300223
45	40.41202575723577
46	37.81478157810728
47	37.7513721683733
48	37.86890584154488
49	40.494566750132535
50	40.56253832302018
51	40.638184001249144
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	45.0
2	44.0
3	87.5
4	131.0
5	252.5
6	374.0
7	877.0
8	1380.0
9	2647.0
10	3914.0
11	6789.0
12	9664.0
13	15534.5
14	21405.0
15	31497.0
16	41589.0
17	57932.0
18	74275.0
19	98671.0
20	123067.0
21	154229.5
22	185392.0
23	222424.0
24	259456.0
25	296176.0
26	371052.5
27	409209.0
28	443365.0
29	477521.0
30	509524.0
31	541527.0
32	562508.5
33	583490.0
34	603899.5
35	624309.0
36	637690.5
37	651072.0
38	657756.5
39	664441.0
40	661770.0
41	659099.0
42	649300.5
43	639502.0
44	616533.0
45	593564.0
46	552574.0
47	511584.0
48	469313.5
49	427043.0
50	385849.5
51	344656.0
52	302818.0
53	260980.0
54	231465.5
55	201951.0
56	283320.0
57	364689.0
58	233862.5
59	103036.0
60	84478.5
61	65921.0
62	54248.5
63	42576.0
64	34716.5
65	26857.0
66	21813.0
67	16769.0
68	13401.5
69	10034.0
70	8039.0
71	6044.0
72	4813.5
73	3583.0
74	2852.0
75	1666.0
76	1211.0
77	970.5
78	730.0
79	577.5
80	425.0
81	291.5
82	158.0
83	121.0
84	84.0
85	66.5
86	49.0
87	35.5
88	22.0
89	15.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04880539824526667
2	4.3066753360041184E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	8.613350672008237E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0766688340010296E-5
18	0.0
19	5.598677936805354E-4
20	0.0
21	0.003348440073743202
22	1.0766688340010296E-4
23	0.0029285392284828004
24	0.00838725021686802
25	0.0
26	0.041462516797379646
27	0.03571310522381415
28	0.05705268151371455
29	0.6269119619737794
30	0.05999198743053736
31	0.009646952752649224
32	0.09069858257624672
33	0.03417346879119267
34	0.017000600888876255
35	0.010109920351269668
36	0.017775802449356996
37	0.03674670730445514
38	0.03317216677557172
39	0.025226350780644124
40	0.0335705342441521
41	0.022308578240501333
42	0.021597976810060652
43	0.02341754713952239
44	0.014028994907033413
45	0.00619084579550592
46	0.0346149030131331
47	0.014298162115533673
48	0.014976463480954322
49	0.015891631989855194
50	0.018443337126437635
51	0.01676373374539603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9287907.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	14.987597602524882
#Duplication Level	Relative count
1	100.0
2	7.767326479754316
3	1.0785588183536652
4	0.5466590221445156
5	0.43993846538110026
6	0.39282247489512434
7	0.34513882187317285
8	0.34059752158536793
9	0.3042671192829287
10++	2.317198471852453
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC	218624	2.3538564716464108	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3814250	3.4279227	3.6232622	3
TTTTT	3851445	3.3731549	3.6561723	5
GTGGC	527780	2.7729576	60.745415	34
CACGT	785955	2.5407634	38.86751	14
CGTGG	481935	2.5320878	60.93336	33
GAGCA	740130	2.489049	41.17301	9
GGAAG	705650	2.4560187	42.791542	5
CTCCA	783795	2.4482293	37.882607	24
CGGAA	709180	2.384964	41.66402	4
GGCCA	467040	2.3832483	58.400063	36
TGGCC	465595	2.3636417	57.93553	35
TCCAG	715315	2.312405	38.99145	25
GAAGA	1042375	2.310253	27.893745	6
CTGAA	1039195	2.2139804	26.612173	19
TCGGA	614325	2.0553308	41.032406	3
TCTCG	624450	2.008271	37.90778	41
AGCAC	615790	2.0009725	39.614143	10
AGAGC	589540	1.9826164	40.734016	8
CCATC	626480	1.9568468	36.253468	38
GCACA	597065	1.9401264	39.415024	11
CACAC	606750	1.9050275	38.070934	12
ATCGG	547915	1.8331447	40.187225	2
TCTGA	853830	1.8096985	26.119131	18
CCAGT	554265	1.7917771	38.34144	26
ACGTG	527690	1.765478	39.075653	32
GTCAC	536805	1.735334	38.236134	29
CTCGT	538365	1.7314163	37.388317	42
GTCTG	513355	1.7086749	39.875423	17
CGTCT	528665	1.7002205	38.603317	16
AAGAG	754080	1.6712945	27.239668	7
ACTCC	530760	1.6578597	37.290203	23
GCCAT	512710	1.6574419	37.14452	37
ACACG	508755	1.6531684	39.07172	13
TGAAC	775495	1.6521738	26.034481	20
ATGCC	503505	1.6276848	37.370773	47
GATCG	483195	1.6166126	39.577644	1
TCACG	500055	1.6165322	37.9838	30
ACGTC	495760	1.6026475	38.731762	15
CAGTC	489670	1.5829602	38.161175	27
AACTC	724145	1.490683	24.99923	22
GAACT	690750	1.4716265	25.78085	21
CATCT	715935	1.466194	24.097904	39
ATCTC	696305	1.4259932	24.29403	40
AGTCA	628115	1.3381841	25.360622	28
TCGTA	564475	1.1964086	24.573133	43
TATGC	512275	1.0857704	24.417978	46
GTATG	491920	1.0790615	25.25489	45
CGTAT	508855	1.0785217	24.420204	44
>>END_MODULE