FastQCFastQC Report
Wed 23 Jul 2014
LW224_GTGAAA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW224_GTGAAA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8965702
Filtered Sequences 0
Sequence length 51
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG 279804 3.1208264561994143 TruSeq Adapter, Index 1 (97% over 35bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3606995 3.3680084 4.113982 1
AAAAA 3604525 3.2965405 3.5382955 2
CACGT 943985 3.1574132 53.574253 14
GAGCA 807855 2.77536 55.97389 9
GGAAG 770695 2.7308104 57.894814 5
CTCCA 841885 2.7302098 51.61545 24
CGGAA 769275 2.6428196 56.32474 4
TCCAG 782000 2.6156106 53.319977 25
TGAAA 1791655 2.5842493 24.247047 35
GAAGA 1089865 2.4587564 37.501366 6
CTGAA 1078025 2.3678381 35.943527 19
TCGGA 682135 2.3532047 56.14283 3
TCTCG 691120 2.321257 52.380486 43
AGCAC 689635 2.2971117 54.080296 10
AGAGC 663575 2.2796907 55.585125 8
GCACA 665190 2.2156878 53.8496 11
CACAC 674880 2.1795475 52.047676 12
ATCGG 621265 2.1432176 55.090775 2
CCAGT 626495 2.0954819 52.475925 26
ACGTG 599895 2.0694962 54.017437 32
GAAAC 940980 2.0582592 34.236073 36
CTCGT 608610 2.0441315 51.971127 44
GTCAC 607610 2.032316 52.796238 29
GTCTG 584995 2.0264926 55.026497 17
GTGAA 893925 2.0251038 36.0642 34
CGTCT 599410 2.0132318 53.33622 16
TCTGA 910455 2.008099 35.76545 18
CGTGA 571375 1.9711089 53.87411 33
ACACG 585015 1.948632 53.369442 13
ACTCC 600465 1.9472915 51.08366 23
GATCG 559160 1.9289699 54.267654 1
TCACG 576010 1.9266212 52.56071 30
ACGTC 570465 1.9080745 53.14582 15
CAGTC 564085 1.8867348 52.34157 27
TGAAC 841040 1.8473102 35.49324 20
AAGAG 818190 1.845852 36.89543 7
CGATC 550005 1.8396404 50.218002 40
AAACG 826060 1.8068883 33.95805 37
AACTC 789590 1.6815189 34.161858 22
GAACT 757690 1.6642354 35.24877 21
AACGA 746085 1.6319544 33.56402 38
ATCTC 757875 1.6206944 33.266632 42
ACGAT 705435 1.5494593 33.55546 39
AGTCA 695710 1.5280988 34.79288 28
GATCT 689595 1.5209702 34.0768 41
TCGTA 629890 1.3892848 34.425083 45
GTATG 563830 1.2826195 35.270107 47
CGTAT 578170 1.2752113 34.284485 46
Produced by FastQC (version 0.10.1)