##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW224_GTGAAA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8965702	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74315240457468	34.0	31.0	34.0	30.0	34.0
2	32.050639091060575	34.0	31.0	34.0	30.0	34.0
3	32.45843024896433	34.0	31.0	34.0	30.0	34.0
4	36.09477696225014	37.0	35.0	37.0	35.0	37.0
5	36.06272559583176	37.0	35.0	37.0	35.0	37.0
6	36.076499754285834	37.0	35.0	37.0	35.0	37.0
7	36.0507141548983	37.0	35.0	37.0	35.0	37.0
8	36.065605905705986	37.0	35.0	37.0	35.0	37.0
9	37.585197790424	39.0	38.0	39.0	35.0	39.0
10	37.72889339841989	39.0	38.0	39.0	35.0	39.0
11	37.75086825326115	39.0	38.0	39.0	35.0	39.0
12	37.72000508158759	39.0	38.0	39.0	35.0	39.0
13	37.71342957863199	39.0	38.0	39.0	35.0	39.0
14	39.164641653269314	40.0	39.0	41.0	36.0	41.0
15	39.114481498492815	40.0	39.0	41.0	36.0	41.0
16	39.12672080780735	40.0	39.0	41.0	36.0	41.0
17	39.08642647279599	40.0	38.0	41.0	36.0	41.0
18	39.0264407628092	40.0	38.0	41.0	36.0	41.0
19	39.08912486718831	40.0	39.0	41.0	36.0	41.0
20	39.06134567042268	40.0	39.0	41.0	36.0	41.0
21	39.00500206230365	40.0	39.0	41.0	36.0	41.0
22	38.92748152905372	40.0	38.0	41.0	35.0	41.0
23	38.86549987942941	40.0	38.0	41.0	35.0	41.0
24	38.80573188803286	40.0	38.0	41.0	35.0	41.0
25	38.76433869874328	40.0	38.0	41.0	35.0	41.0
26	38.68338876308849	40.0	38.0	41.0	35.0	41.0
27	38.590398498634016	40.0	38.0	41.0	34.0	41.0
28	38.44872269901453	40.0	38.0	41.0	34.0	41.0
29	38.150825334145615	40.0	38.0	41.0	34.0	41.0
30	38.064726108451964	40.0	38.0	41.0	33.0	41.0
31	38.362435757958494	40.0	38.0	41.0	34.0	41.0
32	38.36702892868846	40.0	38.0	41.0	34.0	41.0
33	38.41399636079807	40.0	38.0	41.0	34.0	41.0
34	38.5355412214236	40.0	38.0	41.0	34.0	41.0
35	38.48982031747207	40.0	38.0	41.0	34.0	41.0
36	38.54766921764743	40.0	38.0	41.0	34.0	41.0
37	38.536606614852914	40.0	38.0	41.0	34.0	41.0
38	38.50637406864516	40.0	38.0	41.0	34.0	41.0
39	38.42850286569864	40.0	38.0	41.0	34.0	41.0
40	38.32349034130289	40.0	38.0	41.0	34.0	41.0
41	38.30812746174254	40.0	38.0	41.0	34.0	41.0
42	38.193064636767986	40.0	38.0	41.0	33.0	41.0
43	38.19350085470162	40.0	38.0	41.0	33.0	41.0
44	38.16804997533936	40.0	38.0	41.0	33.0	41.0
45	38.07380347908061	40.0	38.0	41.0	33.0	41.0
46	38.06423668776856	40.0	38.0	41.0	33.0	41.0
47	37.956253509206526	40.0	37.0	41.0	33.0	41.0
48	37.81212224095782	40.0	37.0	41.0	33.0	41.0
49	37.77002637384111	40.0	37.0	41.0	33.0	41.0
50	37.723004846692426	40.0	37.0	41.0	33.0	41.0
51	36.6728966677679	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	3.0
12	5.0
13	13.0
14	19.0
15	32.0
16	80.0
17	171.0
18	384.0
19	891.0
20	1731.0
21	2645.0
22	4653.0
23	7875.0
24	12578.0
25	19109.0
26	28256.0
27	39593.0
28	54939.0
29	73566.0
30	97238.0
31	124573.0
32	160030.0
33	206971.0
34	274515.0
35	379984.0
36	563905.0
37	927059.0
38	1926834.0
39	4056745.0
40	1297.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.75980038510231	26.35177773648892	34.179353226752276	21.709068651656494
2	18.037610002565334	32.57682054942726	30.44945737644579	18.936112071561617
3	18.24005526839951	29.75691139411058	33.02445251916693	18.978580818322982
4	18.051704149881402	28.185634543731208	30.50968011205369	23.252981194333692
5	22.231711471115144	29.062788390691548	30.931264501095395	17.77423563709791
6	22.20329205677369	29.737649098754343	28.88821198830833	19.170846856163635
7	19.16214703544686	33.12257088178929	29.876734694059653	17.8385473887042
8	18.24207407295045	33.681467441144044	29.651208572401806	18.425249913503706
9	21.86093528042887	29.2286622254424	30.089498907837157	18.82090358629157
10	18.275557229093717	33.110625358728186	29.55476325222498	19.05905415995312
11	21.98189277314816	29.218559795986977	29.607185248851682	19.192362182013188
12	18.219666457796613	29.31802774618206	29.47252763921888	22.989778156802444
13	18.163418770777792	33.34418208412459	29.500288990198424	18.99211015489919
14	18.223313690327874	29.7164906886265	29.474568751002433	22.585626870043193
15	18.12410227330777	33.45654361476659	29.52161470457082	18.897739407354827
16	18.17276550124017	29.63462314495842	29.598440813669697	22.594170540131717
17	22.043806497249182	29.522283921549032	29.61410049095988	18.8198090902419
18	18.33857516120879	29.343379916040035	33.34872160596014	18.969323316791034
19	18.326149984574442	29.336516001865785	29.578116101445946	22.759217912113822
20	18.42787101333504	29.361225702125722	33.18960411577365	19.02129916876559
21	22.21123922255978	29.30197757571884	29.394779089454303	19.092004112267077
22	18.435772197764166	33.10088047890128	29.43224999216457	19.031097331169974
23	18.453518923614443	33.12444536848948	29.446321039122353	18.975714668773726
24	18.477053990701975	29.364509241248715	29.412619016757997	22.745817751291312
25	18.407861425686466	29.37356160175745	33.1599466500225	19.058630322533585
26	18.441607625578595	29.389785889903386	29.44979590124258	22.718810583275438
27	18.550079750244215	29.412291746979445	29.32560694147911	22.712021561297227
28	18.488208621866672	33.117561788236536	29.305869390026317	19.088360199870475
29	22.322394223070386	29.376763800056953	29.309537207376586	18.991304769496075
30	18.680402540705902	29.26804987440552	32.90979306570276	19.141754519185817
31	18.557759579226165	29.241604066385747	29.31767924740452	22.882957106983568
32	18.59690730868336	33.02429789936657	29.240750790320586	19.138044001629485
33	18.66364491030351	29.377416581292977	29.23036035024822	22.728578158155294
34	22.29270223422667	29.41458839194537	29.279750328142768	19.012959045685193
35	18.683178590288144	29.451159507555925	32.949961594038164	18.91570030811777
36	22.295788944435476	29.38778656559922	29.273920585199964	19.042503904765336
37	18.62745995045454	33.11919497216889	29.268208046306103	18.98513703107046
38	18.663613854095164	33.18608172748638	29.20354930185936	18.946755116559093
39	18.7074951363246	33.08321208079179	29.18196315544148	19.02732962744213
40	18.68983507039458	29.436518687592404	29.250031603725326	22.62361463828769
41	22.320591655006545	29.368673441770394	29.236751029329504	19.073983873893553
42	18.751258555528	32.9773957012373	29.188670819216593	19.082674924018107
43	18.601216839581827	29.361933466524643	32.95613522680974	19.080714467083794
44	18.63171721397197	29.350612842501565	29.394274559018335	22.62339538450812
45	18.655879430189948	29.436073833794207	32.926071728980276	18.98197500703557
46	18.568503191816614	29.42737427518432	29.307697191315192	22.696425341683877
47	22.365767123493967	29.430740493052216	29.26913395427339	18.934358429180428
48	18.78375333266401	29.39286949343507	32.88329261626674	18.940084557634172
49	18.705193511599365	32.986879092858146	29.311253610594896	18.996673784947596
50	18.78700592987343	29.390228733251632	32.84245144570995	18.980313891164986
51	22.40011815933371	29.29884059500453	29.22207941909159	19.078961826570165
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.468869036758804
2	36.973722074126954
3	37.21863608672249
4	41.304685344215095
5	40.00594710821305
6	41.374138912937326
7	37.00069442415106
8	36.66732398645415
9	40.68183886672044
10	37.334611389046835
11	41.17425495516135
12	41.20944461459906
13	37.15552892567698
14	40.80894056037106
15	37.0218416806626
16	40.766936041371885
17	40.86361558749109
18	37.30789847799983
19	41.08536789668827
20	37.44917018210063
21	41.30324333482685
22	37.46686952893414
23	37.429233592388165
24	41.22287174199329
25	37.46649174822005
26	41.16041820885403
27	41.26210131154144
28	37.57656882173715
29	41.31369899256646
30	37.82215705989172
31	41.44071668620973
32	37.734951310312844
33	41.392223068458804
34	41.30566127991186
35	37.598878898405914
36	41.33829284920081
37	37.612596981525
38	37.610368970654264
39	37.734824763766724
40	41.31344970868227
41	41.3945755289001
42	37.83393347954611
43	37.68193130666562
44	41.255112598480096
45	37.63785443722551
46	41.26492853350049
47	41.300125552674395
48	37.723837890298185
49	37.70186729654696
50	37.76731982103841
51	41.47907998590388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	32.5
2	33.0
3	71.0
4	109.0
5	249.5
6	390.0
7	809.5
8	1229.0
9	2416.5
10	3604.0
11	6360.0
12	9116.0
13	14652.5
14	20189.0
15	29963.5
16	39738.0
17	55541.0
18	71344.0
19	94026.5
20	116709.0
21	146325.5
22	175942.0
23	211198.0
24	246454.0
25	282687.5
26	355056.5
27	391192.0
28	424405.0
29	457618.0
30	487034.0
31	516450.0
32	536500.0
33	556550.0
34	575892.5
35	595235.0
36	608781.0
37	622327.0
38	626494.0
39	630661.0
40	627849.5
41	625038.0
42	615125.5
43	605213.0
44	582587.0
45	559961.0
46	523297.0
47	486633.0
48	460447.5
49	434262.0
50	526281.5
51	618301.0
52	437723.0
53	257145.0
54	220255.0
55	183365.0
56	156891.0
57	130417.0
58	112035.5
59	93654.0
60	79553.0
61	65452.0
62	54933.0
63	44414.0
64	36959.5
65	29505.0
66	24989.5
67	20474.0
68	17096.0
69	13718.0
70	11478.5
71	9239.0
72	7718.5
73	6198.0
74	5057.0
75	3127.0
76	2338.0
77	1835.0
78	1332.0
79	1037.0
80	742.0
81	496.5
82	251.0
83	197.5
84	144.0
85	110.0
86	76.0
87	59.0
88	42.0
89	30.0
90	18.0
91	12.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04960013170190131
2	2.2307232607106504E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.692169782131952E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	6.692169782131952E-4
20	0.0
21	0.003691846996476127
22	1.4499701194619228E-4
23	0.0032345487280304435
24	0.008789049647199962
25	0.0
26	0.04309757339692977
27	0.03733115376799274
28	0.05910301279252869
29	0.6360349697101242
30	0.06151219391409619
31	0.009882104044948182
32	0.09214002428365342
33	0.03506696965837142
34	0.018024243946542055
35	0.010573628255768483
36	0.01845923498238063
37	0.03823459668858055
38	0.03388468633019478
39	0.025831775359029335
40	0.03514504497249629
41	0.02342259423746183
42	0.022898374271194826
43	0.024024889517853705
44	0.014800848834815167
45	0.006011799187615203
46	0.036260406602851625
47	0.014912384997850699
48	0.01563737005758166
49	0.016273126186884194
50	0.019708440008378597
51	0.017120801025954244
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	8965702.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	16.090533408411993
#Duplication Level	Relative count
1	100.0
2	7.786099784461922
3	1.2164536877484518
4	0.6392211056705281
5	0.5203623769470936
6	0.40264105232627573
7	0.3804616723252521
8	0.33667161437451304
9	0.29231285437246574
10++	2.1661861134333114
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG	279804	3.1208264561994143	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3606995	3.3680084	4.113982	1
AAAAA	3604525	3.2965405	3.5382955	2
CACGT	943985	3.1574132	53.574253	14
GAGCA	807855	2.77536	55.97389	9
GGAAG	770695	2.7308104	57.894814	5
CTCCA	841885	2.7302098	51.61545	24
CGGAA	769275	2.6428196	56.32474	4
TCCAG	782000	2.6156106	53.319977	25
TGAAA	1791655	2.5842493	24.247047	35
GAAGA	1089865	2.4587564	37.501366	6
CTGAA	1078025	2.3678381	35.943527	19
TCGGA	682135	2.3532047	56.14283	3
TCTCG	691120	2.321257	52.380486	43
AGCAC	689635	2.2971117	54.080296	10
AGAGC	663575	2.2796907	55.585125	8
GCACA	665190	2.2156878	53.8496	11
CACAC	674880	2.1795475	52.047676	12
ATCGG	621265	2.1432176	55.090775	2
CCAGT	626495	2.0954819	52.475925	26
ACGTG	599895	2.0694962	54.017437	32
GAAAC	940980	2.0582592	34.236073	36
CTCGT	608610	2.0441315	51.971127	44
GTCAC	607610	2.032316	52.796238	29
GTCTG	584995	2.0264926	55.026497	17
GTGAA	893925	2.0251038	36.0642	34
CGTCT	599410	2.0132318	53.33622	16
TCTGA	910455	2.008099	35.76545	18
CGTGA	571375	1.9711089	53.87411	33
ACACG	585015	1.948632	53.369442	13
ACTCC	600465	1.9472915	51.08366	23
GATCG	559160	1.9289699	54.267654	1
TCACG	576010	1.9266212	52.56071	30
ACGTC	570465	1.9080745	53.14582	15
CAGTC	564085	1.8867348	52.34157	27
TGAAC	841040	1.8473102	35.49324	20
AAGAG	818190	1.845852	36.89543	7
CGATC	550005	1.8396404	50.218002	40
AAACG	826060	1.8068883	33.95805	37
AACTC	789590	1.6815189	34.161858	22
GAACT	757690	1.6642354	35.24877	21
AACGA	746085	1.6319544	33.56402	38
ATCTC	757875	1.6206944	33.266632	42
ACGAT	705435	1.5494593	33.55546	39
AGTCA	695710	1.5280988	34.79288	28
GATCT	689595	1.5209702	34.0768	41
TCGTA	629890	1.3892848	34.425083	45
GTATG	563830	1.2826195	35.270107	47
CGTAT	578170	1.2752113	34.284485	46
>>END_MODULE