##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW223_GTCCGC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6725563	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60972100030882	33.0	31.0	34.0	30.0	34.0
2	31.90644470953584	34.0	31.0	34.0	30.0	34.0
3	32.34346938687512	34.0	31.0	34.0	30.0	34.0
4	36.02068168865566	37.0	35.0	37.0	35.0	37.0
5	35.999448075945466	37.0	35.0	37.0	35.0	37.0
6	36.00841282729788	37.0	35.0	37.0	35.0	37.0
7	35.98215182877627	37.0	35.0	37.0	35.0	37.0
8	36.01411287055076	37.0	35.0	37.0	35.0	37.0
9	37.48110262293283	39.0	37.0	39.0	35.0	39.0
10	37.61156054890869	39.0	37.0	39.0	35.0	39.0
11	37.638900267531504	39.0	37.0	39.0	35.0	39.0
12	37.59034507594383	39.0	37.0	39.0	35.0	39.0
13	37.60370782341939	39.0	37.0	39.0	35.0	39.0
14	39.05424690840008	40.0	38.0	41.0	36.0	41.0
15	38.97332580186967	40.0	38.0	41.0	36.0	41.0
16	39.03617005743608	40.0	38.0	41.0	36.0	41.0
17	38.98420444504051	40.0	38.0	41.0	36.0	41.0
18	38.883376306191764	40.0	38.0	41.0	35.0	41.0
19	38.98423522313299	40.0	38.0	41.0	36.0	41.0
20	38.96295001622912	40.0	38.0	41.0	35.0	41.0
21	38.89372057030764	40.0	38.0	41.0	35.0	41.0
22	38.82686341649019	40.0	38.0	41.0	35.0	41.0
23	38.78487481271085	40.0	38.0	41.0	35.0	41.0
24	38.71836484172403	40.0	38.0	41.0	35.0	41.0
25	38.66547038515586	40.0	38.0	41.0	34.0	41.0
26	38.58502790026649	40.0	38.0	41.0	34.0	41.0
27	38.50301409710979	40.0	38.0	41.0	34.0	41.0
28	38.251242461040064	40.0	38.0	41.0	34.0	41.0
29	38.01428534681781	40.0	38.0	41.0	33.0	41.0
30	37.86549810030774	40.0	38.0	41.0	33.0	41.0
31	38.19488970663125	40.0	38.0	41.0	34.0	41.0
32	38.1689647394575	40.0	38.0	41.0	34.0	41.0
33	38.272193718206196	40.0	38.0	41.0	34.0	41.0
34	38.33943983574312	40.0	38.0	41.0	34.0	41.0
35	38.21280062353144	40.0	38.0	41.0	33.0	41.0
36	38.32620049801035	40.0	38.0	41.0	34.0	41.0
37	38.32693069710298	40.0	38.0	41.0	34.0	41.0
38	38.27803962285388	40.0	38.0	41.0	34.0	41.0
39	38.15741730469256	40.0	38.0	41.0	33.0	41.0
40	38.111888328159296	40.0	38.0	41.0	33.0	41.0
41	38.11545442366684	40.0	38.0	41.0	33.0	41.0
42	37.94590148066415	40.0	38.0	41.0	33.0	41.0
43	38.019930673461836	40.0	38.0	41.0	33.0	41.0
44	37.987754482412846	40.0	37.0	41.0	33.0	41.0
45	37.86178763026977	40.0	37.0	41.0	33.0	41.0
46	37.852508258416435	40.0	37.0	41.0	33.0	41.0
47	37.66094496475611	40.0	37.0	41.0	32.0	41.0
48	37.37735651275588	40.0	37.0	41.0	31.0	41.0
49	37.32614563271506	40.0	36.0	41.0	31.0	41.0
50	37.36314476572444	40.0	36.0	41.0	31.0	41.0
51	36.28226722432011	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	2.0
13	4.0
14	6.0
15	31.0
16	52.0
17	130.0
18	339.0
19	792.0
20	1458.0
21	2418.0
22	4052.0
23	6950.0
24	11016.0
25	16616.0
26	24084.0
27	34146.0
28	46470.0
29	62386.0
30	81943.0
31	105002.0
32	133057.0
33	171136.0
34	225923.0
35	311424.0
36	458023.0
37	741374.0
38	1463172.0
39	2822865.0
40	684.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.235076827139977	26.1449692399876	27.666160297181168	18.953793635691255
2	18.311745797340684	35.59782281423875	28.221131821975348	17.869299566445218
3	17.793365403015333	26.95713355149599	37.29246161250738	17.957039432981297
4	17.818181169368273	26.892410345423873	28.325242065236765	26.964166419971086
5	26.547115832533276	27.588203396503758	28.058840575874466	17.8058401950885
6	26.80125069083436	27.768738468437515	27.30265109404224	18.127359746685894
7	17.9260977854196	36.71814538054286	27.587073379581756	17.768683454455783
8	17.663235033260413	36.73946701562382	27.632511954761256	17.964785996354507
9	26.557066903851307	27.77659324231938	27.68529975636237	17.981040097466945
10	17.67257254151065	36.63938617480797	27.608736993468057	18.079304290213326
11	26.499967363327055	27.70437210981445	27.666620623433307	18.129039903425184
12	17.700257956099737	27.714081334157452	27.68928043644822	26.89638027329459
13	17.55641869684367	36.58841646416813	27.70001559720725	18.15514924178095
14	17.59391741628173	27.805285594678097	27.852389457953187	26.748407531086986
15	17.58878773420158	36.506624055116276	27.801613039681584	18.102975171000555
16	17.661718431601933	27.843735907313633	27.815084030883362	26.679461630201068
17	26.435571267416574	27.687674622927478	27.794654514425037	18.082099595230915
18	17.805736114582526	27.591400154901528	36.47953338627562	18.123330344240326
19	17.794859312758074	27.603526923787765	27.736215528878727	26.865398234575434
20	17.84614908818786	27.53381389781049	36.35243621983765	18.267600794164
21	26.614288458713315	27.572221141727766	27.61583247538108	18.197657924177836
22	17.919606634694087	36.388461621382916	27.541176899155534	18.15075484476746
23	17.844560429871027	36.34550149389639	27.60602173918862	18.203916337043964
24	17.89959915140306	27.593467522520132	27.656069911254793	26.850863414822012
25	17.89044809793562	27.508054718409365	36.26582228605167	18.335674897603337
26	17.887116809346498	27.57391533158279	27.684270134396648	26.854697724674065
27	17.947620343050893	27.564727165798608	27.590994188978886	26.896658302171613
28	18.02514890722028	36.25103264704425	27.3900023550229	18.333816090712567
29	26.700616534507244	27.670862823652936	27.485389303637227	18.143131338202593
30	18.14328013051705	27.485645475300874	36.078915444507494	18.292158949674587
31	18.033394365156276	27.552157970363012	27.468469080687658	26.945978583793057
32	18.008848635649315	36.19550335730273	27.416632934901664	18.37901507214629
33	18.111667483227777	27.60607188296017	27.386448179306704	26.895812454505354
34	26.630010308671242	27.510217957312705	27.546786079903207	18.31298565411285
35	18.087319818560545	27.621753700582076	36.081909468232034	18.20901701262534
36	18.03336710582807	27.540956399575457	27.5694644895825	26.856212005013973
37	18.064854105704775	27.56942794871608	27.519094182597637	26.84662376298151
38	26.655157622443852	27.529490996232862	27.44407300583276	18.371278375490522
39	18.123874732040107	27.642425870254318	27.445056881709778	26.788642515995797
40	18.02568034470501	36.213852155819275	27.353835765375706	18.406631734100007
41	18.04981200002439	27.674432196648436	27.49653750786524	26.77921829546193
42	18.072510439132106	36.06701924298112	27.42122578417297	18.439244533713808
43	18.03499987581946	27.561030273133234	36.01501826048794	18.388951590559362
44	18.01178347785096	27.46582360175295	27.640999483989546	26.88139343640654
45	18.017697051740413	27.51264351271667	36.11344396617611	18.356215469366806
46	18.146679842743797	27.5310056258121	27.53096100484048	26.79135352660362
47	26.67927310536855	27.64056362558639	27.480167611306893	18.199995657738164
48	18.161951015240465	27.618662823698376	36.0517545637603	18.16763159730086
49	18.12512101159897	36.05729265340999	27.568992597584796	18.248593737406242
50	18.14692640028267	27.56711797642863	35.9805324705685	18.305423152720202
51	26.69756953330882	27.378764046932186	27.46641354293271	18.457252876826285
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	46.188870462831225
2	36.1810453637859
3	35.75040483599663
4	44.78234758933936
5	44.35295602762178
6	44.92861043752025
7	35.69478123987538
8	35.62802102961492
9	44.53810700131825
10	35.751876831723976
11	44.62900726675224
12	44.59663822939432
13	35.71156793862462
14	44.342324947368716
15	35.69176290520213
16	44.341180061803
17	44.517670862647485
18	35.92906645882285
19	44.66025754733351
20	36.11374988235186
21	44.81194638289116
22	36.070361479461546
23	36.04847676691499
24	44.750462566225075
25	36.226122995538965
26	44.74181453402056
27	44.8442786452225
28	36.358964997932844
29	44.84374787270984
30	36.435439080191635
31	44.97937294894933
32	36.38786370779561
33	45.00747993773312
34	44.94299596278409
35	36.29633683118589
36	44.88957911084204
37	44.91147786868628
38	45.026435997934385
39	44.912517248035904
40	36.43231207880501
41	44.82903029548632
42	36.511754972845914
43	36.423951466378824
44	44.8931769142575
45	36.373912521107215
46	44.938033369347416
47	44.879268763106715
48	36.32958261254133
49	36.37371474900522
50	36.452349553002875
51	45.154822410135104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	53.5
2	35.0
3	76.0
4	117.0
5	276.5
6	436.0
7	876.0
8	1316.0
9	2443.0
10	3570.0
11	6149.5
12	8729.0
13	13544.5
14	18360.0
15	26463.5
16	34567.0
17	47152.5
18	59738.0
19	76918.5
20	94099.0
21	115759.0
22	137419.0
23	162307.5
24	187196.0
25	211797.0
26	259112.5
27	281827.0
28	301103.5
29	320380.0
30	335949.0
31	351518.0
32	360620.5
33	369723.0
34	379333.0
35	388943.0
36	394428.5
37	399914.0
38	400742.0
39	401570.0
40	396795.5
41	392021.0
42	386601.0
43	381181.0
44	367921.0
45	354661.0
46	335568.5
47	316476.0
48	297512.5
49	278549.0
50	262445.5
51	246342.0
52	252727.0
53	259112.0
54	465487.5
55	671863.0
56	407747.0
57	143631.0
58	123798.0
59	103965.0
60	92131.0
61	80297.0
62	69311.0
63	58325.0
64	50522.5
65	42720.0
66	37188.5
67	31657.0
68	27213.5
69	22770.0
70	19590.0
71	16410.0
72	13857.5
73	11305.0
74	9421.0
75	6106.5
76	4676.0
77	3831.0
78	2986.0
79	2314.0
80	1642.0
81	1225.0
82	808.0
83	595.5
84	383.0
85	275.5
86	168.0
87	128.0
88	88.0
89	61.5
90	35.0
91	23.5
92	12.0
93	11.5
94	11.0
95	6.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047564791230117094
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.4868643710571145E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	6.24483035843988E-4
20	0.0
21	0.003672554996511073
22	1.1894914968456916E-4
23	0.0029291228109825157
24	0.008623813352131264
25	2.973728742114229E-5
26	0.04017507530596323
27	0.034257355109155924
28	0.055906100351747506
29	0.618386891922654
30	0.0593110197614683
31	0.009753830274134671
32	0.08747223094929005
33	0.03338010513023222
34	0.016756961461813682
35	0.010036334504635524
36	0.017188152129420244
37	0.03630922794121474
38	0.032041927196280816
39	0.023923647730308972
40	0.031714817034648256
41	0.02022135544637676
42	0.01999832579071819
43	0.022035329979066435
44	0.013277698833540031
45	0.005575741391464179
46	0.03370721529186479
47	0.014229292031016586
48	0.01338177933951403
49	0.014883512354281717
50	0.01687591061149825
51	0.015641813183520842
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6725563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.91396336679203
#Duplication Level	Relative count
1	100.0
2	20.336878405939768
3	3.8223732041408507
4	1.2024937752128009
5	0.6614037458099301
6	0.4722474216191524
7	0.3538638717854684
8	0.282447708570583
9	0.1917298796219447
10++	1.3543335456516563
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG	487960	7.255303385010296	TruSeq Adapter, Index 1 (97% over 36bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCACA	1402825	5.73166	113.91549	11
CACGT	1359380	5.6376667	115.309	14
ACGTC	1341970	5.5654635	115.00791	15
TCCGC	744210	4.392114	158.10748	35
CCGCA	739315	4.2985897	156.3224	36
GTCCG	686020	4.2379556	165.96603	34
CGCAC	704570	4.0965724	156.03256	37
CGTCC	691665	4.0820084	158.83286	33
TTTTT	2794705	4.0786905	4.350996	2
GGAAG	903660	4.0454326	126.5805	5
CGGAA	900395	3.8508046	121.065125	4
GAGCA	893825	3.8227065	119.76279	9
AAAAA	2801695	3.7948756	4.020324	14
TCCAG	898565	3.7265592	114.0598	25
CTCCA	898745	3.5608487	108.25491	24
TCGGA	816895	3.5462246	122.40545	3
TCTCG	835625	3.5176415	115.70882	43
GTCTG	786435	3.465329	122.43281	17
ATCGG	792635	3.4409099	120.5413	2
AGAGC	803055	3.4345014	119.228584	8
CCAGT	804230	3.33533	113.402374	26
AGCAC	810750	3.312561	114.0047	10
GAAGA	1099670	3.304922	85.51058	6
CGTCT	780765	3.286703	116.59589	16
GATCG	753565	3.271303	119.423096	1
CACAC	837080	3.2674005	108.45242	12
GTCAC	787110	3.2643292	114.62164	29
CTCGT	770040	3.2415552	115.1185	44
CTGAA	1110550	3.2365108	81.93332	19
TCACG	765505	3.1747284	114.247154	30
CAGTC	747635	3.1006172	113.835434	27
ACACG	754510	3.0827758	113.62868	13
ACTCC	761375	3.0165856	108.22198	23
TCTGA	984995	2.9137652	82.836006	18
AAGAG	922805	2.7733767	84.66174	7
TGAAC	939395	2.7377083	81.15906	20
GAACT	878735	2.560925	80.91406	21
CATCT	885855	2.5034661	77.024025	41
ATCTC	883820	2.497715	77.43722	42
AACTC	886705	2.4687474	76.81365	22
CACAT	880775	2.4522371	75.52941	39
AGTCA	839910	2.4477763	80.555405	28
ACATC	848845	2.3633382	75.70396	40
GTATG	750235	2.3230543	84.26392	47
TCGTA	764050	2.2601762	80.84955	45
CGTAT	743855	2.2004366	80.68423	46
>>END_MODULE