##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW221_CCGTCC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5170297	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557641853069562	33.0	31.0	34.0	30.0	34.0
2	31.844775261459834	34.0	31.0	34.0	30.0	34.0
3	32.29121286456078	34.0	31.0	34.0	30.0	34.0
4	35.98837977779613	37.0	35.0	37.0	35.0	37.0
5	35.962872539043694	37.0	35.0	37.0	35.0	37.0
6	35.950803213045596	37.0	35.0	37.0	35.0	37.0
7	35.88935877378031	37.0	35.0	37.0	35.0	37.0
8	35.9616449499903	37.0	35.0	37.0	35.0	37.0
9	37.39946912140637	39.0	37.0	39.0	35.0	39.0
10	37.599810223668	39.0	37.0	39.0	35.0	39.0
11	37.52732367212174	39.0	37.0	39.0	35.0	39.0
12	37.52173966021681	39.0	37.0	39.0	35.0	39.0
13	37.607685786715926	39.0	37.0	39.0	35.0	39.0
14	38.93746239335961	40.0	38.0	41.0	36.0	41.0
15	38.94442659676997	40.0	38.0	41.0	36.0	41.0
16	38.97425022198918	40.0	38.0	41.0	36.0	41.0
17	38.89783043411239	40.0	38.0	41.0	35.0	41.0
18	38.70584010937863	40.0	38.0	41.0	35.0	41.0
19	38.86028346147233	40.0	38.0	41.0	35.0	41.0
20	38.86319006432319	40.0	38.0	41.0	35.0	41.0
21	38.75110772166473	40.0	38.0	41.0	34.0	41.0
22	38.77117039891519	40.0	38.0	41.0	35.0	41.0
23	38.7983317012543	40.0	38.0	41.0	35.0	41.0
24	38.5133852078517	40.0	38.0	41.0	34.0	41.0
25	38.46164117844681	40.0	38.0	41.0	34.0	41.0
26	38.40907940104795	40.0	38.0	41.0	34.0	41.0
27	38.35559717362465	40.0	38.0	41.0	34.0	41.0
28	38.08496436471638	40.0	38.0	41.0	33.0	41.0
29	37.8764444286276	40.0	38.0	41.0	33.0	41.0
30	37.54058113102594	40.0	37.0	41.0	32.0	41.0
31	37.961521359411265	40.0	38.0	41.0	33.0	41.0
32	38.03672090790916	40.0	38.0	41.0	33.0	41.0
33	38.13299274683833	40.0	38.0	41.0	33.0	41.0
34	38.23695621354054	40.0	38.0	41.0	34.0	41.0
35	38.282219570751934	40.0	38.0	41.0	34.0	41.0
36	38.18239938634086	40.0	38.0	41.0	33.0	41.0
37	37.91468149702038	40.0	38.0	41.0	33.0	41.0
38	37.9564926347558	40.0	38.0	41.0	33.0	41.0
39	37.76388319665195	40.0	37.0	41.0	32.0	41.0
40	37.73073248983569	40.0	37.0	41.0	32.0	41.0
41	37.529657580599334	40.0	37.0	41.0	31.0	41.0
42	36.75557052138397	40.0	36.0	41.0	30.0	41.0
43	36.96550314227596	40.0	36.0	41.0	30.0	41.0
44	37.17136520397184	40.0	36.0	41.0	31.0	41.0
45	37.09964340539818	40.0	36.0	41.0	31.0	41.0
46	37.127272379130254	40.0	36.0	41.0	31.0	41.0
47	36.74621032408776	39.0	35.0	41.0	30.0	41.0
48	36.39194963074655	39.0	35.0	41.0	29.0	41.0
49	36.34775855236169	39.0	35.0	41.0	29.0	41.0
50	36.51086194854957	39.0	35.0	41.0	30.0	41.0
51	35.313534599656464	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	0.0
13	4.0
14	9.0
15	21.0
16	41.0
17	140.0
18	316.0
19	777.0
20	1466.0
21	2754.0
22	4768.0
23	8088.0
24	12650.0
25	18401.0
26	25912.0
27	35067.0
28	46337.0
29	60184.0
30	77028.0
31	95279.0
32	118358.0
33	148937.0
34	192638.0
35	260937.0
36	381767.0
37	607496.0
38	1136665.0
39	1933811.0
40	439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.53149843603514	23.282168672134336	27.698132046428427	20.488200845402098
2	18.033265026463674	36.71123214439408	26.806787620435585	18.44871520870666
3	18.07478371165138	24.876888116872202	38.70060075852509	18.347727412951325
4	17.54077183573787	24.280810173960994	26.929826274970274	31.24859171533086
5	29.89522652180329	24.91382990184123	26.929555497488828	18.26138807886665
6	30.293791633246602	25.708658516135536	25.05680041204596	18.940749438571906
7	18.920673222447377	37.45984031478269	25.851106812626046	17.76837965014389
8	18.26419255992451	37.83169516180598	25.727554916090895	18.176557362178613
9	29.93198449913467	25.71081091744915	25.982981232007784	18.374223351408393
10	18.32639401566293	37.505543685401435	25.61448597633753	18.553576322598104
11	30.112912275639097	25.590135344255856	25.610018147893634	18.68693423221142
12	18.226825267484635	25.709064682357706	25.591760009144544	30.47235004101312
13	18.228952804838872	37.61215264809739	25.599012977397624	18.559881569666114
14	18.216361651951523	25.94655587483659	25.568163685761185	30.268918787450698
15	18.136134152448108	37.68675184423642	25.65595361349648	18.521160389819
16	18.210211134872907	25.863330481788573	25.680942506784426	30.24551587655409
17	30.0499951936997	25.77300685047687	25.709606237320603	18.467391718502824
18	18.33917858103703	25.679414548139114	37.42075165121076	18.56065521961311
19	18.325971353847333	25.63407860884312	25.69378549387671	30.34616454343284
20	18.415827949535586	25.59226288161009	37.30899791636728	18.68291125248704
21	30.150105181555205	25.672674536005825	25.560491192833883	18.61672908960509
22	18.39883967778971	37.37464914913831	25.601185079051692	18.625326094020295
23	18.378745139422936	37.4206408766601	25.576383591403605	18.624230392513358
24	18.387304866384067	25.68998550249475	25.634954945544898	30.28775468557629
25	18.334111173884207	25.637231284779194	37.30048776695033	18.72816977438627
26	18.378495831191252	25.685747803468978	25.657556513668133	30.278199851671634
27	18.446552915022917	25.700242039867895	25.505197762630672	30.348007282478516
28	18.496121552803157	37.275395682116724	25.48169996818597	18.746782796894145
29	30.164728923540622	25.73243342964965	25.513814593167357	18.589023053642375
30	18.63682308177757	25.608591880822797	37.003369459893634	18.751215577505995
31	18.466979831888885	25.62623856458525	25.54093537558066	30.36584622794521
32	18.485520194954663	37.232813754919505	25.444282033845283	18.837384016280552
33	18.505320039120356	25.687155193845996	25.435465444171758	30.3720593228619
34	18.487372850273992	25.61592077835054	25.498559240591344	30.398147130784125
35	18.544807854975193	25.680860393758465	25.474682962788446	30.299648788477896
36	30.101188577446337	25.676778044579923	25.556339863161643	18.665693514812098
37	18.55044109304707	25.66696023855166	37.048004420604414	18.734594247796856
38	18.55324745627872	25.67595725584755	25.560144925994067	30.210650361879672
39	18.554845629232737	25.715538216561125	25.43982189483972	30.289794259366413
40	18.57192082362499	25.70486797069184	25.43371467630991	30.28949652937326
41	30.03675397355548	25.684525588592667	25.479853216248355	18.798867221603498
42	18.81128743018867	36.55885044174345	25.52574918412393	19.104112943943946
43	18.515514263201872	25.803202848558517	36.87208744235823	18.809195445881393
44	18.463154103379857	25.68756549037237	25.698823499372203	30.15045690687557
45	18.547204565399085	25.64719781494033	36.969767422518174	18.835830197142407
46	18.56655271613277	25.645719154659748	25.702117220138387	30.0856109090691
47	29.88145752959983	25.744077325546304	25.596871261817107	18.777593883036754
48	18.73359646054949	25.775628209135842	36.82232877645071	18.668446553863962
49	18.63942588459776	36.712323096406976	25.833529514060498	18.81472150493476
50	18.701029436116	25.792736997024047	36.80909237223741	18.697141194622542
51	29.905420454661957	25.577274136760714	25.600835359475976	18.916470049101356
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	49.01969928143724
2	36.48198023517033
3	36.42251112460271
4	48.78936355106873
5	48.15661460066995
6	49.234541071818505
7	36.68905287259127
8	36.44074992210312
9	48.30620785054307
10	36.87997033826103
11	48.79984650785051
12	48.69917530849775
13	36.78883437450499
14	48.485280439402224
15	36.65729454226711
16	48.455727011427
17	48.51738691220253
18	36.89983380065014
19	48.67213589728017
20	37.09873920202263
21	48.76683427116029
22	37.024165771810004
23	37.00297553193629
24	48.67505955196035
25	37.06228094827048
26	48.65669568286289
27	48.79456019750143
28	37.242904349697305
29	48.75375197718299
30	37.388038659283566
31	48.8328260598341
32	37.32290421123521
33	48.87737936198226
34	48.88551998105811
35	48.844456643453086
36	48.766882092258435
37	37.285035340843926
38	48.76389781815839
39	48.84463988859915
40	48.861417352998245
41	48.83562119515897
42	37.915400374132616
43	37.324709709083265
44	48.613611010255426
45	37.38303476254149
46	48.652163625201865
47	48.659051412636586
48	37.40204301441345
49	37.454147389532515
50	37.39817063073854
51	48.82189050376331
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	36.5
2	24.0
3	52.0
4	80.0
5	191.5
6	303.0
7	653.5
8	1004.0
9	1816.0
10	2628.0
11	4305.0
12	5982.0
13	9103.5
14	12225.0
15	17230.0
16	22235.0
17	29989.5
18	37744.0
19	48237.5
20	58731.0
21	71786.0
22	84841.0
23	99675.0
24	114509.0
25	129363.0
26	158168.0
27	172119.0
28	184610.5
29	197102.0
30	207744.5
31	218387.0
32	225346.5
33	232306.0
34	240908.5
35	249511.0
36	255498.5
37	261486.0
38	264767.0
39	268048.0
40	269534.0
41	271020.0
42	270194.5
43	269369.0
44	266179.5
45	262990.0
46	256567.5
47	250145.0
48	242011.0
49	233877.0
50	225709.5
51	217542.0
52	207232.0
53	196922.0
54	204544.5
55	212167.0
56	433434.5
57	654702.0
58	405122.5
59	155543.0
60	128634.0
61	101725.0
62	87856.5
63	73988.0
64	64714.5
65	55441.0
66	48369.5
67	41298.0
68	35682.5
69	30067.0
70	25917.5
71	21768.0
72	18379.5
73	14991.0
74	12516.5
75	8067.5
76	6093.0
77	4807.5
78	3522.0
79	2714.0
80	1906.0
81	1401.0
82	896.0
83	652.5
84	409.0
85	312.0
86	215.0
87	146.0
88	77.0
89	54.0
90	31.0
91	20.0
92	9.0
93	7.0
94	5.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04529720439657529
2	3.8682497349765396E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	9.67062433744135E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.543086983204253E-4
20	0.0
21	0.0036554959995528306
22	1.740712380739443E-4
23	0.0024950210790598685
24	0.008548831914298154
25	0.0
26	0.03934009980471141
27	0.03340233646152242
28	0.05347855258605067
29	0.6125953692795598
30	0.058023746024648104
31	0.009709306834791116
32	0.08467598669863646
33	0.031564917837408564
34	0.015492340188581042
35	0.00924511686659393
36	0.016246648886901468
37	0.03429203390056703
38	0.030752585393063494
39	0.023518958388657363
40	0.031603600334758335
41	0.020501723595375665
42	0.019476637415606877
43	0.021004596060922615
44	0.012455764146624458
45	0.00537686713161739
46	0.032493297773802936
47	0.013016660358196055
48	0.01317139034759512
49	0.014389889014112728
50	0.016730180103773536
51	0.015202221458457803
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5170297.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	35.46150766943355
#Duplication Level	Relative count
1	100.0
2	25.72861855557676
3	5.396139480356608
4	1.5083562801603096
5	0.722484255295946
6	0.5118732789879767
7	0.4137244744949426
8	0.28694893535810684
9	0.21947163226914584
10++	1.5301671256032061
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG	475635	9.199374813477833	TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTATG	10360	0.20037533627178475	TruSeq Adapter, Index 7 (97% over 36bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG	6651	0.12863864493664484	TruSeq Adapter, Index 2 (97% over 34bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGAAG	849930	4.635663	157.61714	5
CCGTC	725890	4.396942	168.18602	34
CGGAA	852395	4.3473387	148.24825	4
TCCCG	713965	4.324709	165.35805	37
GAGCA	835160	4.259437	146.86069	9
ACCCG	711095	4.254722	167.89746	32
TTTTT	1770600	4.2517858	5.2224197	2
CCCGT	701510	4.249265	168.70135	33
TCCAG	869915	4.200003	137.16167	25
CGTCC	688415	4.1699443	166.98465	35
GTCTG	769955	4.024582	149.56287	17
CCCGA	672235	4.022209	156.1738	38
GTCCC	663910	4.02151	166.06627	36
ATCGG	778795	4.0210743	147.25307	2
CACCC	715835	4.005072	157.25146	31
TCGGA	772730	3.9897594	149.54405	3
AAAAA	1763250	3.9818296	4.2240825	13
GAAGA	971625	3.9497907	117.92391	6
GATCG	759865	3.9233353	145.76192	1
AGAGC	766885	3.9112248	146.54695	8
CCAGT	798465	3.8550382	136.18881	26
CTGAA	991745	3.8165033	110.9956	19
TCACC	842100	3.8018093	127.52778	30
CTCCA	832980	3.7606354	128.15189	24
AGCAC	783610	3.7371142	137.3312	10
GCACA	779920	3.7195165	137.18788	11
TCTCG	758850	3.7090707	132.00851	43
CGTCT	757435	3.702155	139.76846	16
GTCAC	755625	3.648204	136.49715	29
CACGT	741885	3.5818667	138.31041	14
ACGTC	738260	3.5643647	138.25426	15
CCGAT	734295	3.5452216	125.51653	39
CAGTC	729690	3.5229883	135.9744	27
CGATC	722915	3.4902782	125.54836	40
ACACG	728245	3.4730732	136.75008	13
CTCGT	708940	3.4651234	131.00159	44
TCTGA	882685	3.4388068	112.01653	18
CACAC	768125	3.4254858	128.00569	12
AAGAG	838240	3.4075623	117.258675	7
ACTCC	738065	3.3321254	128.38824	23
TGAAC	864445	3.3266187	110.44723	20
GAACT	828730	3.1891778	110.253845	21
ATTTT	1266355	3.0037925	3.4866662	1
AGTCA	776245	2.9872012	108.7304	28
AACTC	826435	2.9739127	102.938866	22
GATCT	756180	2.945963	101.65083	41
GTATG	680440	2.8349023	109.078094	47
ATCTC	777445	2.832211	95.17083	42
TCGTA	695970	2.7113934	102.79783	45
CGTAT	686665	2.675143	102.58612	46
CCCGC	145370	1.091253	7.487457	38
CCGCT	165265	1.0010617	5.9359083	39
CGCTC	141510	0.8571702	5.83621	40
>>END_MODULE