##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW220_ATGTCA_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6968942	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60139028277176	34.0	31.0	34.0	31.0	34.0
2	32.827330317858866	34.0	31.0	34.0	31.0	34.0
3	32.92793181518802	34.0	31.0	34.0	31.0	34.0
4	36.340245477721005	37.0	37.0	37.0	35.0	37.0
5	36.26752339164252	37.0	37.0	37.0	35.0	37.0
6	36.24743798986991	37.0	37.0	37.0	35.0	37.0
7	36.215032497041875	37.0	37.0	37.0	35.0	37.0
8	36.20383868885693	37.0	37.0	37.0	35.0	37.0
9	37.991164512489846	39.0	38.0	39.0	35.0	39.0
10	37.95269568895824	39.0	38.0	39.0	35.0	39.0
11	37.94479807695343	39.0	38.0	39.0	35.0	39.0
12	37.92053628800469	39.0	38.0	39.0	35.0	39.0
13	37.909693465665235	39.0	38.0	39.0	35.0	39.0
14	39.42661109821261	41.0	39.0	41.0	36.0	41.0
15	39.35684111017139	41.0	39.0	41.0	36.0	41.0
16	39.36562336148012	41.0	39.0	41.0	36.0	41.0
17	39.34856036970892	41.0	39.0	41.0	36.0	41.0
18	39.296330920819834	40.0	39.0	41.0	36.0	41.0
19	39.357057498828375	40.0	39.0	41.0	36.0	41.0
20	39.3392070130588	40.0	39.0	41.0	36.0	41.0
21	39.29063837810675	40.0	39.0	41.0	36.0	41.0
22	39.195057298511024	40.0	39.0	41.0	36.0	41.0
23	39.126603866124874	40.0	39.0	41.0	36.0	41.0
24	39.079173280535265	40.0	39.0	41.0	36.0	41.0
25	39.06591861433199	40.0	39.0	41.0	36.0	41.0
26	38.96445457574478	40.0	39.0	41.0	36.0	41.0
27	38.87489205678566	40.0	38.0	41.0	35.0	41.0
28	38.71370876669658	40.0	38.0	41.0	35.0	41.0
29	38.4389166963938	40.0	38.0	41.0	34.0	41.0
30	38.4061544779681	40.0	38.0	41.0	34.0	41.0
31	38.6656680454508	40.0	38.0	41.0	35.0	41.0
32	38.626728992722285	40.0	38.0	41.0	35.0	41.0
33	38.69058416614746	40.0	38.0	41.0	35.0	41.0
34	38.81032414963419	40.0	38.0	41.0	35.0	41.0
35	38.86642620931556	40.0	38.0	41.0	35.0	41.0
36	38.87183032948187	40.0	38.0	41.0	35.0	41.0
37	38.81551632945144	40.0	38.0	41.0	35.0	41.0
38	38.76929826650875	40.0	38.0	41.0	35.0	41.0
39	38.73239266448193	40.0	38.0	41.0	35.0	41.0
40	38.67222112050868	40.0	38.0	41.0	35.0	41.0
41	38.59776290289114	40.0	38.0	41.0	35.0	41.0
42	38.54852989162487	40.0	38.0	41.0	34.0	41.0
43	38.544890888746096	40.0	38.0	41.0	34.0	41.0
44	38.48298880949217	40.0	38.0	41.0	34.0	41.0
45	38.39267696588664	40.0	38.0	41.0	34.0	41.0
46	38.36888196228352	40.0	38.0	41.0	34.0	41.0
47	38.226798702012445	40.0	38.0	41.0	34.0	41.0
48	38.05865180109119	40.0	38.0	41.0	33.0	41.0
49	37.99399765416329	40.0	37.0	41.0	33.0	41.0
50	37.938813380854654	40.0	37.0	41.0	33.0	41.0
51	36.92099819456095	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	2.0
14	7.0
15	12.0
16	34.0
17	78.0
18	196.0
19	456.0
20	987.0
21	1709.0
22	2949.0
23	4808.0
24	7553.0
25	11465.0
26	16699.0
27	24129.0
28	33149.0
29	44831.0
30	59744.0
31	77249.0
32	100141.0
33	130479.0
34	175160.0
35	247039.0
36	375150.0
37	640674.0
38	1412656.0
39	3599907.0
40	1675.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.68248243454147	27.003910836769187	29.981392623117564	21.332214105571776
2	19.266913112492542	32.1049163560265	29.26234713963755	19.36582339184341
3	18.497398887808224	29.471589805166982	33.127869337985594	18.903141969039204
4	18.14400234641069	29.00565107300362	30.428492588975487	22.421853991610206
5	21.773132851442874	29.510060494118047	30.655413691203055	18.061392963236024
6	21.90079928918909	29.770932230459085	29.46312367070927	18.865144809642555
7	18.74010430851627	33.0721506937495	29.99783898330622	18.189906014428015
8	18.412292712437555	33.30402233222776	29.74417063594445	18.539514319390232
9	21.765972510604907	29.306543231382896	30.038906910116342	18.88857734789585
10	18.471627400543728	32.99965762378278	29.54824419546037	18.980470780213125
11	21.926929510964506	29.3978626884827	29.595798042227933	19.079409758324864
12	18.370765605453453	29.50694667856326	29.5849068624764	22.53738085350689
13	18.33992878689477	33.12542994331133	29.575565415812044	18.95907585398185
14	18.33670017629649	29.742176072063735	29.64871855727885	22.27240519436092
15	18.301056315291476	33.16814804887169	29.70838328113507	18.82241235470176
16	18.33782205933441	29.689231118472666	29.75021599408002	22.22273082811291
17	21.851667010573482	29.592339841542664	29.700376326851334	18.855616821032516
18	18.490425088915934	29.44926216920732	33.122100887049996	18.938211854826744
19	18.518930932116806	29.395922921684186	29.593026859859904	22.4921192863391
20	18.62700417969113	29.388761785156298	32.96480266141058	19.019431373741988
21	22.067439432587474	29.360943391488092	29.483811020345197	19.087806155579234
22	18.58771944551948	32.867439630912656	29.518262726049997	19.026578197517864
23	18.57338849433622	32.87800298840583	29.538108631550912	19.010499885707038
24	18.598475923605047	29.41617249792006	29.540079397991832	22.445272180483062
25	18.571874837224193	29.416721995494004	32.96562562374972	19.04577754353208
26	18.623468991399285	29.423883411078904	29.53920581049131	22.413441787030504
27	18.670316965055676	29.421713291789743	29.46559959008819	22.442370153066392
28	18.71986910520273	32.88575229320113	29.317470152407065	19.076908449189077
29	22.201947693642737	29.476952320791366	29.380826107492307	18.940273878073587
30	18.76101384548469	29.357733723353462	32.75516928930496	19.126083141856892
31	18.74906444336551	29.380791032778458	29.337018104913522	22.533126418942512
32	18.704283921396744	32.787417550752004	29.358114769554273	19.150183758296986
33	18.712958343404395	29.44930470912378	29.351833086556407	22.485903860915414
34	18.69402448031457	32.80228412909399	29.44489733716777	19.058794053423675
35	18.729599830534088	29.482734296883667	32.80616346141013	18.981502411172123
36	22.167793440044935	29.452979258271267	29.40849097612601	18.970736325557784
37	18.75246857447287	29.518610755177114	32.75937541621314	18.96954525413687
38	18.733811900780243	29.557313153640468	29.385850241660965	22.32302470391832
39	18.76854770808844	32.874241606020746	29.293377796408503	19.063832889482317
40	22.18687579985176	29.46495981866295	29.328730244486945	19.01943413699835
41	18.82231329942598	32.80227341252831	29.343935588558484	19.03147769948723
42	18.793798736230993	32.757163099231015	29.366180687046654	19.082857477491338
43	18.740198385819088	29.369008530130998	32.75755427482406	19.13323880922586
44	18.768222076515784	29.312323664269403	29.477693135351203	22.441761123863614
45	18.72728345044881	29.374051033160402	32.82786342425478	19.070802092136006
46	18.77235447390875	29.429047307057775	29.40426102949883	22.394337189534653
47	22.16895498140789	29.488562731683793	29.379952095361517	18.962530191546797
48	18.859865833646065	29.475095128379365	32.70437264062605	18.960666397348533
49	18.83273842783334	32.73877230393272	29.386255307538324	19.04223396069562
50	18.903201543355188	29.382456966342456	32.656804427959536	19.05753706234282
51	22.258123226101112	29.306654028033307	29.316715750456652	19.118506995408925
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.01469654011324
2	38.63273650433595
3	37.40054085684743
4	40.56585633802089
5	39.8345258146789
6	40.765944098831646
7	36.930010322944284
8	36.95180703182779
9	40.65454985850076
10	37.45209818075685
11	41.00633926928937
12	40.908146458960346
13	37.29900464087662
14	40.60910537065741
15	37.12346866999324
16	40.560552887447315
17	40.707283831606
18	37.42863694374268
19	41.01105021845591
20	37.64643555343312
21	41.15524558816671
22	37.61429764303735
23	37.583888380043255
24	41.04374810408811
25	37.61765238075627
26	41.036910778429785
27	41.11268711812207
28	37.7967775543918
29	41.14222157171633
30	37.887096987341586
31	41.28219086230802
32	37.85446767969373
33	41.198862204319816
34	37.75281853373824
35	37.71110224170621
36	41.13852976560272
37	37.72201382860975
38	41.05683660469857
39	37.83238059757076
40	41.206309936850104
41	37.853790998913205
42	37.876656213722335
43	37.87343719504494
44	41.2099832003794
45	37.79808554258482
46	41.1666916634434
47	41.13148517295469
48	37.820532230994594
49	37.87497238852895
50	37.96073860569801
51	41.37663022151004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	13.5
2	14.0
3	42.5
4	71.0
5	206.0
6	341.0
7	775.0
8	1209.0
9	2422.0
10	3635.0
11	6216.5
12	8798.0
13	13802.5
14	18807.0
15	27614.0
16	36421.0
17	49728.5
18	63036.0
19	82450.5
20	101865.0
21	126004.5
22	150144.0
23	177772.5
24	205401.0
25	233078.5
26	287158.0
27	313560.0
28	336890.5
29	360221.0
30	379806.5
31	399392.0
32	411424.5
33	423457.0
34	437895.5
35	452334.0
36	459575.5
37	466817.0
38	467558.0
39	468299.0
40	463082.5
41	457866.0
42	448622.0
43	439378.0
44	423314.5
45	407251.0
46	388966.0
47	370681.0
48	442692.0
49	514703.0
50	387800.0
51	260897.0
52	231003.0
53	201109.0
54	177823.0
55	154537.0
56	136227.5
57	117918.0
58	104128.5
59	90339.0
60	79079.0
61	67819.0
62	57821.5
63	47824.0
64	40382.5
65	32941.0
66	28281.5
67	23622.0
68	19885.5
69	16149.0
70	13682.0
71	11215.0
72	9434.0
73	7653.0
74	6342.5
75	4138.0
76	3244.0
77	2590.0
78	1936.0
79	1557.5
80	1179.0
81	864.5
82	550.0
83	414.0
84	278.0
85	210.0
86	142.0
87	96.0
88	50.0
89	33.0
90	16.0
91	13.0
92	10.0
93	9.0
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04914662799604301
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.434938043680088E-5
17	0.0
18	0.0
19	4.304814131040264E-4
20	8.609628262080528E-5
21	0.006701160663986011
22	0.0026546353808081626
23	1.2914442393120794E-4
24	0.0
25	1.434938043680088E-5
26	0.07156036023832599
27	0.07882114673934723
28	0.09189343231727283
29	0.6863739144334965
30	0.1040330081668064
31	0.016717028208873025
32	0.08206410671806423
33	0.025627993460126373
34	0.00228155148945134
35	0.016774425730620227
36	0.011694745055992719
37	0.019486458633175597
38	0.0012627454784384774
39	0.010948577273279072
40	0.029071844764958582
41	0.01060419214279585
42	0.026488956286334422
43	0.010073265066634217
44	0.030391987765144266
45	0.0025685390981873574
46	0.019759096861474812
47	0.01337362256709842
48	0.01043199957755424
49	0.024810078775228724
50	0.03303227376551563
51	0.02789519556914091
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6968942.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	34.972302142826464
#Duplication Level	Relative count
1	100.0
2	31.734501456485212
3	7.383682963158629
4	1.8475716686110928
5	0.8159252166384174
6	0.6376250119233676
7	0.5415043254308921
8	0.5121544974942585
9	0.4226375222875256
10++	2.8535370211392137
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG	206779	2.9671505373412494	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3007545	3.6153114	4.023951	4
AAAAA	2983205	3.5065615	3.7603533	3
GGAAG	589460	2.6547909	53.49294	5
CTCCA	607415	2.564248	48.498676	24
GAGCA	580330	2.554082	51.823895	9
TCCAG	587415	2.5376735	49.75191	25
CGGAA	568865	2.5036237	52.129726	4
CTGAA	820200	2.3172207	33.622032	19
GAAGA	801960	2.3081791	34.77771	6
CAGAA	791065	2.2249117	32.15161	38
TCTCG	505115	2.1919367	49.129234	43
TCGGA	489995	2.1662	52.0443	3
AGAGC	481925	2.1209931	51.34035	8
GCACA	488460	2.1007419	50.001225	11
AGCAC	487220	2.095409	50.000217	10
CACAC	497450	2.0906286	48.536633	12
CCAGT	476640	2.0591178	49.116722	26
ATCGG	458900	2.0287333	51.790028	2
GTCTG	444765	1.9750792	51.23913	17
GTCAG	444355	1.9644318	49.30167	36
GTCAC	451845	1.9520015	49.08911	29
TCTGA	685125	1.9443057	33.45726	18
TCAGA	683465	1.9309183	32.13499	37
CGTCT	439505	1.9072235	49.797787	16
CTCGT	439035	1.9051839	48.59719	44
CACGT	435395	1.8809365	49.660988	14
ACTCC	444935	1.8783267	48.00668	23
GATCG	416660	1.841996	51.360497	1
CAGTC	417550	1.8038447	48.842014	27
ACACG	418840	1.8013238	49.47519	13
ACGTC	412685	1.7828276	49.53236	15
TGAAC	625945	1.7684134	33.004368	20
AAGAG	609470	1.7541598	34.133102	7
TCACA	623950	1.7225875	31.71716	30
TGTCA	578730	1.6423689	32.036816	35
AACTC	583745	1.6115903	31.999853	22
GAATC	569955	1.610231	31.823765	40
GAACT	562200	1.5883217	32.818596	21
ATCTC	567150	1.5728106	31.431057	42
CACAT	565750	1.5619103	31.33347	31
AGTCA	522965	1.4774754	32.275043	28
CATGT	507070	1.4390061	32.053387	33
ACATG	507450	1.4336426	31.968376	32
ATGTC	502045	1.4247457	31.867987	34
AGAAT	766330	1.4158604	21.210466	39
AATCT	708615	1.2851263	20.710442	41
TCGTA	447750	1.2706628	31.726702	45
GTATG	418105	1.2142168	32.19638	47
CGTAT	416335	1.1815107	31.571814	46
>>END_MODULE