FastQCFastQC Report
Wed 23 Jul 2014
LW219_AGTTCC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW219_AGTTCC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6890497
Filtered Sequences 0
Sequence length 51
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG 309667 4.4941170426458354 TruSeq Adapter, Index 8 (97% over 37bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1663975 3.852325 5.6200457 2
AAAAA 1706320 3.8435473 4.197841 12
CGTAT 871295 2.6146784 47.82584 46
TCCAG 772245 2.6119618 55.70075 25
GAAGA 841045 2.5612447 51.482037 6
CTGAA 857935 2.5605106 49.983288 19
GGAAG 709935 2.5137982 59.498253 5
CGGAA 696315 2.4031289 58.025448 4
GAGCA 687585 2.373 57.875553 9
TTCCG 684900 2.3292694 54.478786 36
CCAGT 684370 2.3147428 55.330593 26
CAGTT 759975 2.280617 48.77742 33
GATCG 653690 2.268423 57.896248 1
ATCGG 640265 2.2218356 58.1269 2
AGCAC 646510 2.1747336 55.90676 10
CTCCA 658970 2.1723857 53.936882 24
TGAAC 714545 2.1325624 49.553444 20
GTCTG 601990 2.1004984 57.512367 17
TCTGA 688535 2.066232 49.7944 18
GTTCC 603505 2.052454 54.28831 35
GCACA 609425 2.049987 55.73145 11
TCTCG 590795 2.0092287 54.875748 43
GTCAC 588695 1.9911413 55.039574 29
CACAG 591795 1.9906831 54.25091 31
AGAGC 570885 1.9702438 57.195763 8
AAGAG 646225 1.9679569 50.74707 7
CGTCT 573205 1.9494071 55.91105 16
GAACT 644570 1.9237217 49.283665 21
AGTTC 639505 1.9190973 48.207733 34
TCGGA 552760 1.9181776 57.87924 3
ACGTC 566615 1.9164602 55.699074 15
CACGT 561615 1.8995486 55.70748 14
CACAC 579175 1.8988914 54.19839 12
ATCTC 642640 1.8796644 47.083473 42
CAGTC 548735 1.8559847 54.88128 27
TCCGT 543285 1.8476526 54.176857 37
TCACA 628840 1.8292451 47.12746 30
AACTC 624435 1.8164313 47.850773 22
ACAGT 606325 1.8095794 48.09066 32
CTCGT 531445 1.807386 54.51073 44
ACACG 526140 1.7698324 55.39186 13
CCGTA 522730 1.7680281 53.577755 38
ACTCC 529920 1.7469542 53.80421 23
AGTCA 582360 1.7380557 48.43796 28
GTATC 550480 1.6519413 47.854637 40
GTATG 517400 1.5930153 48.922764 47
TCGTA 527120 1.5818402 47.930664 45
TATCT 592095 1.5365449 41.558052 41
Produced by FastQC (version 0.10.1)