##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW219_AGTTCC_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6890497	
Filtered Sequences	0	
Sequence length	51	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53759053955034	34.0	31.0	34.0	31.0	34.0
2	32.74790250978993	34.0	31.0	34.0	31.0	34.0
3	32.854524572030144	34.0	31.0	34.0	31.0	34.0
4	36.29566851273573	37.0	37.0	37.0	35.0	37.0
5	36.22420748459799	37.0	37.0	37.0	35.0	37.0
6	36.194175978888026	37.0	37.0	37.0	35.0	37.0
7	36.15130432536289	37.0	37.0	37.0	35.0	37.0
8	36.14228465668006	37.0	37.0	37.0	35.0	37.0
9	37.902064974413314	39.0	38.0	39.0	35.0	39.0
10	37.84515587192041	39.0	38.0	39.0	35.0	39.0
11	37.86239555724355	39.0	38.0	39.0	35.0	39.0
12	37.845812283206854	39.0	38.0	39.0	35.0	39.0
13	37.853224520669556	39.0	38.0	39.0	35.0	39.0
14	39.30914286734324	41.0	39.0	41.0	36.0	41.0
15	39.23827889338026	40.0	39.0	41.0	36.0	41.0
16	39.26007703072797	40.0	39.0	41.0	36.0	41.0
17	39.21823229877322	40.0	39.0	41.0	36.0	41.0
18	39.171370512170604	40.0	39.0	41.0	36.0	41.0
19	39.20861064158362	40.0	39.0	41.0	36.0	41.0
20	39.17478115149024	40.0	39.0	41.0	36.0	41.0
21	39.11668156883314	40.0	39.0	41.0	36.0	41.0
22	39.0089283835404	40.0	39.0	41.0	36.0	41.0
23	38.96149319853125	40.0	38.0	41.0	35.0	41.0
24	38.87501148320651	40.0	38.0	41.0	35.0	41.0
25	38.83737384981083	40.0	38.0	41.0	35.0	41.0
26	38.718162274796725	40.0	38.0	41.0	35.0	41.0
27	38.60462721339259	40.0	38.0	41.0	35.0	41.0
28	38.39862581755714	40.0	38.0	41.0	34.0	41.0
29	38.12298967694203	40.0	38.0	41.0	34.0	41.0
30	38.03188492789417	40.0	38.0	41.0	33.0	41.0
31	38.296948536513405	40.0	38.0	41.0	34.0	41.0
32	38.22786077695121	40.0	38.0	41.0	34.0	41.0
33	38.281715963304244	40.0	38.0	41.0	34.0	41.0
34	38.411621832213264	40.0	38.0	41.0	34.0	41.0
35	38.47102161135837	40.0	38.0	41.0	34.0	41.0
36	38.3601682142812	40.0	38.0	41.0	34.0	41.0
37	38.35656092731772	40.0	38.0	41.0	34.0	41.0
38	38.27793481370067	40.0	38.0	41.0	34.0	41.0
39	38.213078534102834	40.0	38.0	41.0	33.0	41.0
40	38.09531170247951	40.0	38.0	41.0	33.0	41.0
41	38.00726841619697	40.0	37.0	41.0	33.0	41.0
42	37.88642706034122	40.0	37.0	41.0	33.0	41.0
43	37.85710885586337	40.0	37.0	41.0	33.0	41.0
44	37.74167872070766	40.0	37.0	41.0	33.0	41.0
45	37.60676464992293	40.0	36.0	41.0	33.0	41.0
46	37.52181243239784	40.0	36.0	41.0	33.0	41.0
47	37.32871141225372	39.0	36.0	41.0	32.0	41.0
48	37.13286356557444	39.0	35.0	41.0	32.0	41.0
49	36.98984920826466	39.0	35.0	41.0	31.0	41.0
50	36.90544644312304	39.0	35.0	41.0	31.0	41.0
51	35.822411503843625	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	1.0
12	1.0
13	3.0
14	7.0
15	17.0
16	57.0
17	153.0
18	327.0
19	821.0
20	1769.0
21	2963.0
22	4874.0
23	7822.0
24	11486.0
25	16675.0
26	23885.0
27	32089.0
28	42851.0
29	56611.0
30	72907.0
31	93149.0
32	119278.0
33	153813.0
34	206774.0
35	295717.0
36	467159.0
37	797630.0
38	1526108.0
39	2954420.0
40	1125.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	26.87601518801859	22.410095377399845	23.732447873765967	26.9814415608156
2	21.618875967872857	30.214685529940727	25.946793097798317	22.219645404388103
3	21.345775203152982	24.170781875385767	33.02972194893925	21.45372097252201
4	20.361738783138573	23.705329238224763	27.292066160104273	28.64086581853239
5	26.22658423623144	24.228847353100942	27.29480906819929	22.24975934246833
6	27.024857568329253	25.958533905464293	24.519044127005643	22.49756439920081
7	22.365237224542728	30.90476637606837	25.386659336764822	21.343337062624073
8	21.639280882061193	31.080457621561987	25.505344534654036	21.77491696172279
9	26.57412085078914	25.52537211757004	26.024218572332302	21.876288459308523
10	21.69514042310736	30.724024696622028	25.538012715193116	22.042822165077496
11	26.857075766813338	25.563206833991803	25.43343390179257	22.14628349740229
12	21.744178975769092	25.650500972571354	25.398196966053394	27.20712308560616
13	21.74034761208081	30.735199507379512	25.334224802652116	22.19022807788756
14	21.64779986117112	25.927041256965932	25.40234761004903	27.022811271813918
15	21.541813311869955	30.938639114130662	25.515721144643123	22.00382642935626
16	21.620965802611916	25.86511539007999	25.451574828346924	27.062343978961167
17	26.77626882356962	25.792072763401535	25.458279714801414	21.973378698227428
18	21.723701497874536	25.73701142312376	30.54097549131797	21.998311587683734
19	21.802810115137365	25.553471354981124	25.39986778831327	27.243850741568238
20	21.824938694481624	25.624799633233575	30.33104036593049	22.21922130635431
21	26.848461142682563	25.651642267832507	25.43112025155243	22.0687763379325
22	21.86066330475071	30.544182510035462	25.373723734336174	22.221430450877662
23	21.797685593759404	30.673422061382254	25.388894524673344	22.139997820185002
24	21.81098745445719	25.73680120223583	25.4327882104261	27.019423132880878
25	21.773741429682065	25.541031365371758	30.405513564551296	22.27971364039488
26	21.809082124144574	25.682344367625525	25.413658883147882	27.094914625082016
27	21.834407167271483	25.75489107370516	25.351727695736624	27.05897406328673
28	21.945722127015383	30.57357287303255	25.184873800662537	22.295831199289527
29	27.02399088677703	25.73415889282991	25.17207842211254	22.069771798280517
30	22.026425318331384	25.55570890020411	30.25854391992504	22.159321861539468
31	21.94783995085737	25.48231802958022	25.249000846816255	27.320841172746164
32	21.94602377944508	30.56799706496893	25.22396895559778	22.262010199988204
33	21.952567764225133	25.68726660400434	25.28603416427449	27.074131467496038
34	21.949337412092397	30.44784060446443	25.327564089446504	22.275257893996674
35	26.89747388616964	25.765220179473936	25.250745461441465	22.08656047291496
36	21.96613953056318	25.698715990214442	30.31496811565595	22.02017636356643
37	21.94983382840315	25.53417897760922	30.30348379739839	22.212503396589245
38	22.013994762304556	25.57509068730784	25.298925249276717	27.11198930111089
39	21.99590176069278	25.625984427967886	25.278600734884733	27.099513076454603
40	26.98739534103189	25.474946592642528	25.188515342239686	22.34914272408589
41	22.117604028555192	25.634911822741245	30.10060502350208	22.146879125201473
42	22.004796204843238	30.51570815151594	25.299167632004504	22.180328011636313
43	21.994706991914477	25.462013363437546	30.18839033220491	22.354889312443067
44	21.950951479171398	25.498422367182332	25.374882866116426	27.17574328752984
45	21.91346741207858	25.554672353090837	30.352570973026104	22.17928926180448
46	22.06683625516658	25.445663336587206	25.380271365792233	27.10722904245398
47	26.99115660792747	25.690381998309352	25.212840568132204	22.105620825630975
48	22.0454478821381	25.643521331307934	30.256642185158235	22.05438860139574
49	22.144466684966833	30.351262994411094	25.271414131897927	22.23285618872415
50	22.132664839516046	25.61309207279974	30.043024998399463	22.21121808928475
51	26.996449965269033	25.45454017578152	25.24699633383246	22.302013525116983
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	53.85745674883419
2	43.838521372260956
3	42.79949617567499
4	49.00260460167097
5	48.47634357869977
6	49.522421967530065
7	43.708574287166805
8	43.41419784378398
9	48.45040931009766
10	43.73796258818486
11	49.00335926421563
12	48.95130206137525
13	43.93057568996837
14	48.67061113298504
15	43.54563974122621
16	48.68330978157309
17	48.74964752179705
18	43.72201308555827
19	49.04666085670561
20	44.04416000083594
21	48.917237480615064
22	44.08209375562837
23	43.9376834139444
24	48.830410587338065
25	44.05345507007695
26	48.90399674922659
27	48.89338123055821
28	44.24155332630491
29	49.09376268505755
30	44.18574717987085
31	49.26868112360353
32	44.20803397943329
33	49.026699231721174
34	44.22459530608907
35	48.984034359084596
36	43.98631589412961
37	44.16233722499239
38	49.125984063415444
39	49.09541483714738
40	49.33653806511778
41	44.264483153756665
42	44.18512421647955
43	44.34959630435755
44	49.12669476670124
45	44.09275667388306
46	49.17406529762056
47	49.096777433558444
48	44.099836483533835
49	44.37732287369098
50	44.343882928800795
51	49.29846349038602
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	11.0
4	20.0
5	49.0
6	78.0
7	217.5
8	357.0
9	757.0
10	1157.0
11	2253.0
12	3349.0
13	5525.0
14	7701.0
15	11632.5
16	15564.0
17	21829.5
18	28095.0
19	37371.0
20	46647.0
21	58689.0
22	70731.0
23	84553.5
24	98376.0
25	114290.5
26	144797.0
27	159389.0
28	174788.5
29	190188.0
30	204566.5
31	218945.0
32	229943.5
33	240942.0
34	254560.5
35	268179.0
36	279731.0
37	291283.0
38	302545.0
39	313807.0
40	323271.5
41	332736.0
42	343511.0
43	354286.0
44	362778.5
45	371271.0
46	376229.5
47	381188.0
48	394238.0
49	407288.0
50	558970.5
51	710653.0
52	545042.5
53	379432.0
54	361300.0
55	343168.0
56	324431.0
57	305694.0
58	284366.0
59	263038.0
60	242359.0
61	221680.0
62	200256.0
63	178832.0
64	161830.5
65	144829.0
66	129656.5
67	114484.0
68	102696.5
69	90909.0
70	80692.5
71	70476.0
72	60832.0
73	51188.0
74	43500.0
75	29404.5
76	22997.0
77	18073.5
78	13150.0
79	10048.5
80	6947.0
81	4999.0
82	3051.0
83	2213.0
84	1375.0
85	975.5
86	576.0
87	413.5
88	251.0
89	173.0
90	95.0
91	70.5
92	46.0
93	33.5
94	21.0
95	13.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04681810325147809
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	4.644077197914751E-4
20	1.1610192994786877E-4
21	0.006995141279359095
22	0.0026122934238270476
23	5.8050964973934385E-5
24	2.9025482486967193E-5
25	0.0
26	0.06895003364779058
27	0.07545174172487122
28	0.08810685208918892
29	0.6794139813136846
30	0.10082001341848056
31	0.016878318066171426
32	0.07823818804362008
33	0.024990940421278758
34	0.0024091150464182775
35	0.0165445250175713
36	0.010913581415099665
37	0.017894209953215275
38	8.99789957095983E-4
39	0.010289533541629871
40	0.02805312882365379
41	0.009897689528055814
42	0.024918376715061336
43	0.00947682003199479
44	0.029199635381888998
45	0.0024381405289052444
46	0.018460206861711136
47	0.012089113455821837
48	0.009738049374377495
49	0.02423627787661761
50	0.03157972494582031
51	0.02658734195806195
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6890497.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	33.23309190679901
#Duplication Level	Relative count
1	100.0
2	28.054359241846466
3	7.190334881688294
4	2.0716002565711102
5	0.885311303931036
6	0.5110276236863066
7	0.3510231125459044
8	0.2699635584439385
9	0.20652564653805217
10++	1.4329919433851879
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG	309667	4.4941170426458354	TruSeq Adapter, Index 8 (97% over 37bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1663975	3.852325	5.6200457	2
AAAAA	1706320	3.8435473	4.197841	12
CGTAT	871295	2.6146784	47.82584	46
TCCAG	772245	2.6119618	55.70075	25
GAAGA	841045	2.5612447	51.482037	6
CTGAA	857935	2.5605106	49.983288	19
GGAAG	709935	2.5137982	59.498253	5
CGGAA	696315	2.4031289	58.025448	4
GAGCA	687585	2.373	57.875553	9
TTCCG	684900	2.3292694	54.478786	36
CCAGT	684370	2.3147428	55.330593	26
CAGTT	759975	2.280617	48.77742	33
GATCG	653690	2.268423	57.896248	1
ATCGG	640265	2.2218356	58.1269	2
AGCAC	646510	2.1747336	55.90676	10
CTCCA	658970	2.1723857	53.936882	24
TGAAC	714545	2.1325624	49.553444	20
GTCTG	601990	2.1004984	57.512367	17
TCTGA	688535	2.066232	49.7944	18
GTTCC	603505	2.052454	54.28831	35
GCACA	609425	2.049987	55.73145	11
TCTCG	590795	2.0092287	54.875748	43
GTCAC	588695	1.9911413	55.039574	29
CACAG	591795	1.9906831	54.25091	31
AGAGC	570885	1.9702438	57.195763	8
AAGAG	646225	1.9679569	50.74707	7
CGTCT	573205	1.9494071	55.91105	16
GAACT	644570	1.9237217	49.283665	21
AGTTC	639505	1.9190973	48.207733	34
TCGGA	552760	1.9181776	57.87924	3
ACGTC	566615	1.9164602	55.699074	15
CACGT	561615	1.8995486	55.70748	14
CACAC	579175	1.8988914	54.19839	12
ATCTC	642640	1.8796644	47.083473	42
CAGTC	548735	1.8559847	54.88128	27
TCCGT	543285	1.8476526	54.176857	37
TCACA	628840	1.8292451	47.12746	30
AACTC	624435	1.8164313	47.850773	22
ACAGT	606325	1.8095794	48.09066	32
CTCGT	531445	1.807386	54.51073	44
ACACG	526140	1.7698324	55.39186	13
CCGTA	522730	1.7680281	53.577755	38
ACTCC	529920	1.7469542	53.80421	23
AGTCA	582360	1.7380557	48.43796	28
GTATC	550480	1.6519413	47.854637	40
GTATG	517400	1.5930153	48.922764	47
TCGTA	527120	1.5818402	47.930664	45
TATCT	592095	1.5365449	41.558052	41
>>END_MODULE