##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW218_AGTCAA_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5380642	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.511315006647905	34.0	31.0	34.0	31.0	34.0
2	32.70476013828833	34.0	31.0	34.0	31.0	34.0
3	32.82606536543409	34.0	31.0	34.0	31.0	34.0
4	36.27426151005772	37.0	37.0	37.0	35.0	37.0
5	36.188787323148425	37.0	37.0	37.0	35.0	37.0
6	36.15693889316554	37.0	37.0	37.0	35.0	37.0
7	36.05700620855281	37.0	35.0	37.0	35.0	37.0
8	36.10399037884327	37.0	36.0	37.0	35.0	37.0
9	37.8441617189919	39.0	38.0	39.0	35.0	39.0
10	37.72678148815699	39.0	38.0	39.0	35.0	39.0
11	37.78112537500172	39.0	38.0	39.0	35.0	39.0
12	37.8001337015174	39.0	38.0	39.0	35.0	39.0
13	37.801960621056	39.0	38.0	39.0	35.0	39.0
14	39.358828741997705	41.0	39.0	41.0	36.0	41.0
15	39.20103121523417	40.0	39.0	41.0	36.0	41.0
16	39.29938360515344	41.0	39.0	41.0	36.0	41.0
17	39.24264186318287	41.0	39.0	41.0	36.0	41.0
18	39.15221046113085	40.0	39.0	41.0	36.0	41.0
19	39.25638260267083	40.0	39.0	41.0	36.0	41.0
20	39.26357579634549	40.0	39.0	41.0	36.0	41.0
21	39.18177905164477	40.0	39.0	41.0	36.0	41.0
22	39.03535024259187	40.0	39.0	41.0	36.0	41.0
23	38.9152510053633	40.0	39.0	41.0	35.0	41.0
24	39.00775260647335	40.0	38.0	41.0	36.0	41.0
25	38.95188102088933	40.0	38.0	41.0	36.0	41.0
26	38.943617694691454	40.0	38.0	41.0	36.0	41.0
27	38.883009685461325	40.0	38.0	41.0	35.0	41.0
28	38.293476131658636	40.0	38.0	41.0	34.0	41.0
29	38.24301579625628	40.0	38.0	41.0	34.0	41.0
30	37.97795040071426	40.0	38.0	41.0	34.0	41.0
31	38.38569077816365	40.0	38.0	41.0	34.0	41.0
32	38.231161262912494	40.0	38.0	41.0	34.0	41.0
33	38.52159519254394	40.0	38.0	41.0	35.0	41.0
34	38.48954622887009	40.0	38.0	41.0	34.0	41.0
35	38.60395004908336	40.0	38.0	41.0	35.0	41.0
36	38.08705596841418	40.0	38.0	41.0	33.0	41.0
37	38.386234393590954	40.0	38.0	41.0	34.0	41.0
38	38.478560365101416	40.0	38.0	41.0	34.0	41.0
39	38.33113576409655	40.0	38.0	41.0	34.0	41.0
40	38.331518060484235	40.0	38.0	41.0	34.0	41.0
41	38.46512795313273	40.0	38.0	41.0	34.0	41.0
42	38.46108977330214	40.0	38.0	41.0	34.0	41.0
43	38.48220249553864	40.0	38.0	41.0	34.0	41.0
44	38.47445918163669	40.0	38.0	41.0	34.0	41.0
45	38.1752396832943	40.0	38.0	41.0	33.0	41.0
46	37.76238597550255	40.0	37.0	41.0	33.0	41.0
47	37.75387435179668	40.0	37.0	41.0	33.0	41.0
48	37.910960439293305	40.0	37.0	41.0	33.0	41.0
49	37.768977939807186	40.0	37.0	41.0	33.0	41.0
50	37.777124923011044	40.0	37.0	41.0	33.0	41.0
51	36.90200909110846	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	8.0
12	4.0
13	6.0
14	10.0
15	21.0
16	26.0
17	70.0
18	169.0
19	330.0
20	802.0
21	1388.0
22	2491.0
23	4261.0
24	6614.0
25	10618.0
26	15417.0
27	22346.0
28	31047.0
29	42052.0
30	55770.0
31	72215.0
32	92845.0
33	117523.0
34	156050.0
35	213902.0
36	316763.0
37	523360.0
38	1099982.0
39	2593350.0
40	1198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.412258341745535	23.52179700604358	25.119126621088	17.946818031122884
2	16.367414892126256	42.038180573990985	25.340526279206088	16.253878254676675
3	15.938191018841247	24.690938367577697	43.202186653562904	16.168683960018154
4	15.932095091998317	24.371998731749855	25.83132273063326	33.86458344561857
5	33.575751741149105	24.981163957758202	25.722172186887736	15.72091211420496
6	33.72337353051922	25.269698299942643	24.695844845280547	16.311083324257588
7	16.401239852047393	42.663849406817995	25.15560410820865	15.77930663292596
8	15.991976422144422	42.74843039176366	25.15515806478112	16.104435121310804
9	33.28273837954653	24.964530254939838	25.437577894979817	16.315153470533815
10	16.098766652752587	42.446551917038896	25.050096252454633	16.40458517775388
11	33.38285654388454	25.00655126284187	25.03989300904985	16.57069918422374
12	16.080107169367523	25.092563303784193	24.967503877790048	33.85982564905824
13	15.968633482770272	42.4262383559434	24.989508686881603	16.615619474404728
14	15.950122680527715	25.2961449581667	25.061953573569845	33.691778787735736
15	15.917803117174492	42.36988820293192	25.166123299041267	16.54618538085232
16	16.123057434410242	25.175620307019127	25.092693399783894	33.60862885878674
17	33.23469578537283	25.018928967955866	25.29367313417247	16.452702112498844
18	16.244827290126345	24.904500243651224	42.26027303061605	16.590399435606386
19	16.109670032020514	24.922569231424003	25.25031999142105	33.71744074513443
20	16.327450387853634	24.911729798585224	42.1530866785686	16.607733134992543
21	33.32446142168171	25.02888327662001	25.06460635104965	16.582048950648627
22	16.27498829109454	42.213038888434575	24.956788563187203	16.555184257283685
23	16.14692375871202	42.09496600869673	24.99077712116456	16.767333111426687
24	16.18840279654361	24.978264675479245	25.154173052211988	33.67915947576516
25	16.20871635763911	24.88069267570673	41.99030524610261	16.92028572055156
26	16.24289477384161	24.88084495747465	25.056536808065722	33.81972346061802
27	16.254274479520625	25.150622745122547	24.903518492273484	33.69158428308335
28	16.58787158410482	41.873075928623116	24.78741510834324	16.751637378928827
29	33.3014441801048	25.044704376697407	25.118031342179098	16.5358201010187
30	16.50122289916119	24.842944847591994	41.71724175266276	16.938590500584056
31	16.29920997849891	25.138264450965636	24.915018921963885	33.64750664857157
32	16.375758811212197	41.79613436394146	24.732772349010563	17.095334475835774
33	16.31450598854714	25.292511917978434	24.834531389772735	33.558450703701695
34	16.646572548007967	41.7360017529927	24.804828089812254	16.81259760918708
35	33.27559209093066	25.095004450022568	25.10827644714957	16.52112701189721
36	16.49808281132406	25.00588753224202	41.616051857123765	16.87997779931015
37	16.296869374062556	25.314243895384447	24.969779683061695	33.419107047491295
38	16.311038363772173	42.18554127433687	24.820971603199734	16.682448758691223
39	16.30110760640661	42.21186713184029	24.83465828511525	16.65236697663785
40	16.36804533985089	41.86091042215356	25.187758790535757	16.58328544745979
41	16.361962173025066	25.01966514971164	41.6460102050603	16.972362472202995
42	16.437374715131746	24.76949815038962	25.34374273247843	33.44938440200021
43	16.395535333750548	24.782760058296194	41.61614881179961	17.20555579615365
44	16.76408069599721	24.831282247482946	25.015086161221706	33.389550895298136
45	33.15030817773593	24.878733366552062	25.252286079785534	16.71867237592647
46	16.604704214177925	25.29356260927853	41.53690180054212	16.564831376001422
47	16.476804554385843	41.66481256262829	25.328436639921097	16.52994624306477
48	16.799780376319305	25.01208153287515	41.574431907737235	16.61370618306831
49	33.21090152456044	24.825549544264046	24.939448677630505	17.02410025354501
50	16.701540282884363	24.78230404575468	24.75216803586278	33.763987635498175
51	16.842205844844884	24.757790883315028	24.75641522327706	33.643588048563025
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	51.359076372868415
2	32.62129314680293
3	32.1068749788594
4	49.796678537616884
5	49.29666385535406
6	50.034456854776806
7	32.180546484973355
8	32.096411543455226
9	49.597891850080345
10	32.503351830506475
11	49.953555728108284
12	49.93993281842576
13	32.584252957174996
14	49.641901468263455
15	32.463988498026815
16	49.73168629319698
17	49.68739789787166
18	32.83522672573273
19	49.82711077715494
20	32.935183522846174
21	49.906510372330345
22	32.83017254837822
23	32.91425687013871
24	49.86756227230877
25	33.129002078190666
26	50.062618234459634
27	49.945858762603976
28	33.33950896303365
29	49.8372642811235
30	33.43981339974525
31	49.94671662707048
32	33.47109328704797
33	49.872956692248835
34	33.459170157195054
35	49.796719102827865
36	33.378060610634215
37	49.71597642155386
38	32.99348712246339
39	32.95347458304445
40	32.95133078731068
41	33.33432464522806
42	49.88675911713195
43	33.6010911299042
44	50.153631591295344
45	49.868980553662404
46	33.16953559017935
47	33.006750797450614
48	33.413486559387614
49	50.23500177810545
50	50.465527918382534
51	50.485793893407916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	36.0
1	34.5
2	33.0
3	65.0
4	97.0
5	216.5
6	336.0
7	698.0
8	1060.0
9	1943.5
10	2827.0
11	4695.5
12	6564.0
13	9987.0
14	13410.0
15	18913.0
16	24416.0
17	33220.5
18	42025.0
19	54171.0
20	66317.0
21	81276.0
22	96235.0
23	112891.0
24	129547.0
25	146262.5
26	179471.0
27	195964.0
28	209508.5
29	223053.0
30	235286.0
31	247519.0
32	255046.5
33	262574.0
34	271543.5
35	280513.0
36	286605.5
37	292698.0
38	294385.5
39	296073.0
40	295158.5
41	294244.0
42	290348.0
43	286452.0
44	278280.0
45	270108.0
46	256720.5
47	243333.0
48	243544.5
49	243756.0
50	649979.0
51	1056202.0
52	619618.0
53	183034.0
54	151308.0
55	119582.0
56	105590.5
57	91599.0
58	81590.0
59	71581.0
60	62823.0
61	54065.0
62	46584.0
63	39103.0
64	33294.0
65	27485.0
66	23309.0
67	19133.0
68	16183.0
69	13233.0
70	11125.5
71	9018.0
72	7405.0
73	5792.0
74	4761.0
75	3035.0
76	2340.0
77	1817.0
78	1294.0
79	989.5
80	685.0
81	498.0
82	311.0
83	227.0
84	143.0
85	108.0
86	73.0
87	56.0
88	39.0
89	28.5
90	18.0
91	12.5
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04722484788989864
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.389691416005748E-4
20	7.43405712552517E-5
21	0.006802162269855531
22	0.002267387423285177
23	1.8585142813812926E-5
24	0.0
25	0.0
26	0.0671667061291199
27	0.07367150611395444
28	0.08682978722613398
29	0.6706820487220669
30	0.09851984205602232
31	0.017005405674638825
32	0.07694249124918551
33	0.025535986226178958
34	0.002397483422981867
35	0.016615117675548755
36	0.011690054829888328
37	0.018269195385978106
38	0.0013195451397807175
39	0.010630701689500992
40	0.028491023933575214
41	0.010519190832618115
42	0.026037785082151908
43	0.009868710834134662
44	0.028621119933271905
45	0.002657675422375248
46	0.019328548526365442
47	0.012730822827461852
48	0.009850125691320849
49	0.024550973657046872
50	0.03150181706941291
51	0.026130710796220973
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5380642.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.926245147707082
#Duplication Level	Relative count
1	100.0
2	9.523837074751215
3	1.4851886137468178
4	0.7289979171488081
5	0.6277482064336959
6	0.5380698912288822
7	0.5201342281879194
8	0.4246702152279565
9	0.3697060865540384
10++	1.4956028697060866
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC	839900	15.609661449321473	TruSeq Adapter, Index 8 (97% over 36bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAGTC	1971945	9.807588	212.029	27
AGTCA	2031650	7.2793884	152.61446	28
GGAAG	1162190	6.398566	242.40463	5
GTCTG	1071915	6.1012473	246.349	17
TCGGA	1101155	5.925962	237.22328	3
CGGAA	1160490	5.904783	224.44383	4
GAGCA	1158085	5.8925457	222.96979	9
TCTCG	1116510	5.873243	223.24428	41
ATCGG	1086145	5.845184	237.1045	2
TCCAG	1155760	5.7482424	213.18939	25
GATCG	1057900	5.693181	236.46698	1
CGTCT	1074385	5.651649	227.8833	16
CTCGT	1067200	5.6138535	222.08456	42
AGAGC	1094060	5.566775	222.66399	8
CCAGT	1086280	5.40268	212.42052	26
CTCCA	1170555	5.380421	197.2602	24
GTCAC	1072110	5.3322043	212.26886	29
CACGT	1066700	5.305297	215.8803	14
ATGCC	1061145	5.277669	209.81055	47
ACGTC	1054020	5.2422323	215.62482	15
GCACA	1104285	5.19279	205.28284	11
CACAG	1101545	5.179905	199.83614	31
AGCAC	1098980	5.167844	205.62286	10
ACACG	1054585	4.95908	204.32898	13
ACTCC	1068075	4.909375	197.79955	23
CACAC	1114465	4.8433175	189.4236	12
GAAGA	1307935	4.794323	161.5724	6
CTGAA	1311660	4.6996694	155.72604	19
TCTGA	1218390	4.617216	164.53592	18
GTATG	1053350	4.3192616	172.7991	45
AAGAG	1177330	4.315582	160.89085	7
TTTTT	2000185	4.3015184	4.7141995	1
TGAAC	1181575	4.233576	155.02429	20
GTCAA	1144030	4.099052	151.13895	35
GAACT	1141685	4.09065	154.69284	21
ACAGT	1136090	4.070603	151.49356	32
TCGTA	1067485	4.045346	159.9144	43
TATGC	1062695	4.0271935	159.46567	46
ATCTC	1149260	4.02503	148.55647	40
CGTAT	1048920	3.9749918	159.77362	44
TCACA	1177565	3.8993115	140.95662	30
AACTC	1158850	3.8373396	143.09499	22
TCAAA	1557805	3.7161632	101.20243	36
CAAAT	1448730	3.4559627	101.463486	37
AAATC	1436135	3.4259176	101.361	38
AAAAA	1997890	3.246265	3.4457302	5
AATCT	1231365	3.1068218	106.81786	39
TGCCG	211470	1.5797217	9.146477	47
>>END_MODULE