FastQCFastQC Report
Wed 23 Jul 2014
LW217_CTTGTA_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW217_CTTGTA_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7224626
Filtered Sequences 0
Sequence length 51
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC 375812 5.2018194436639345 TruSeq Adapter, Index 12 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3046115 3.7252295 3.909003 3
TTTTT 3106060 3.6991026 4.0594277 2
GGAAG 740565 3.2836359 87.58208 5
GAGCA 734965 3.1083007 83.43193 9
CGGAA 731260 3.0926316 83.65235 4
TCCAG 743410 2.9829452 78.029594 25
CTCCA 752655 2.8805628 74.45761 24
TCACC 736515 2.8187919 73.981804 30
GAAGA 965180 2.7857265 57.619495 6
TCGGA 647855 2.7253993 82.784065 3
TCTCG 682720 2.7249308 76.914955 41
GCACA 673150 2.7153933 79.19421 11
AGAGC 641455 2.71283 83.068535 8
CACAC 704420 2.7102983 75.58095 12
CTGAA 972945 2.6642742 54.327663 19
ATCGG 626690 2.6363623 82.585556 2
AGCAC 651335 2.6273947 79.12508 10
CCAGT 653680 2.622902 77.570564 26
GTCTG 621760 2.601783 81.60211 17
GATCG 596410 2.5089803 82.27571 1
GTCAC 624860 2.507261 77.55444 29
CACGT 616240 2.4726732 78.33902 14
ATGCC 615730 2.4706268 76.94274 47
CGTCT 617920 2.4662955 77.807816 16
CTCGT 616025 2.458732 76.32963 42
ACACG 602390 2.4299574 78.72164 13
CCTTG 607785 2.4258437 76.50212 33
CACCT 633365 2.4240158 73.40961 31
CAGTC 596780 2.3945897 77.331566 27
ACGTC 595310 2.3886912 78.105774 15
ACTCC 610210 2.335397 74.28785 23
AAGAG 787765 2.2736666 57.14517 7
TCTGA 833220 2.2695842 53.64557 18
TGAAC 803465 2.2001767 53.84712 20
GAACT 737790 2.020335 53.612408 21
CTTGT 726555 1.9685711 52.11509 34
AACTC 753330 1.9676164 51.10258 22
ATCTC 732720 1.903659 50.07114 40
AGTCA 691960 1.8948361 52.970108 28
ACCTT 665095 1.7279646 49.917465 32
GTATG 601640 1.718141 54.681477 45
TATGC 612415 1.6681397 52.123642 46
TCGTA 608335 1.6570263 52.133205 43
CGTAT 589735 1.6063622 52.121616 44
AATCT 871320 1.5449079 34.26623 39
TTGTA 828555 1.5320667 35.735306 35
TGTAA 770855 1.4329567 35.750088 36
GTAAT 705600 1.311653 35.578503 37
TAATC 690170 1.223717 33.86762 38
Produced by FastQC (version 0.10.1)