##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW217_CTTGTA_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7224626	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5967651474277	34.0	31.0	34.0	31.0	34.0
2	32.816594796741036	34.0	31.0	34.0	31.0	34.0
3	32.92048183532269	34.0	31.0	34.0	31.0	34.0
4	36.336002306555386	37.0	37.0	37.0	35.0	37.0
5	36.26362707218339	37.0	37.0	37.0	35.0	37.0
6	36.24356084314953	37.0	37.0	37.0	35.0	37.0
7	36.18633338251696	37.0	37.0	37.0	35.0	37.0
8	36.183863220047655	37.0	37.0	37.0	35.0	37.0
9	37.98743602783037	39.0	38.0	39.0	35.0	39.0
10	37.91368854249341	39.0	38.0	39.0	35.0	39.0
11	37.93347378812412	39.0	38.0	39.0	35.0	39.0
12	37.91035452907874	39.0	38.0	39.0	35.0	39.0
13	37.899425520435244	39.0	38.0	39.0	35.0	39.0
14	39.4139843363518	41.0	39.0	41.0	36.0	41.0
15	39.333995559078076	41.0	39.0	41.0	36.0	41.0
16	39.36128818294539	41.0	39.0	41.0	36.0	41.0
17	39.34274203259795	41.0	39.0	41.0	36.0	41.0
18	39.26461951109995	40.0	39.0	41.0	36.0	41.0
19	39.34152106974119	40.0	39.0	41.0	36.0	41.0
20	39.33299190850848	40.0	39.0	41.0	36.0	41.0
21	39.28262293439134	40.0	39.0	41.0	36.0	41.0
22	39.172133339497435	40.0	39.0	41.0	36.0	41.0
23	39.13487466340818	40.0	39.0	41.0	36.0	41.0
24	39.090505030987075	40.0	39.0	41.0	36.0	41.0
25	39.07182143407839	40.0	39.0	41.0	36.0	41.0
26	38.97094257889613	40.0	39.0	41.0	36.0	41.0
27	38.886036592067185	40.0	38.0	41.0	35.0	41.0
28	38.635902813515884	40.0	38.0	41.0	35.0	41.0
29	38.40586945261941	40.0	38.0	41.0	34.0	41.0
30	38.33520627919009	40.0	38.0	41.0	34.0	41.0
31	38.625968043190056	40.0	38.0	41.0	35.0	41.0
32	38.58115631175925	40.0	38.0	41.0	35.0	41.0
33	38.67213652305323	40.0	38.0	41.0	35.0	41.0
34	38.81167205056705	40.0	38.0	41.0	35.0	41.0
35	38.86233391181772	40.0	38.0	41.0	35.0	41.0
36	38.87144123446667	40.0	38.0	41.0	35.0	41.0
37	38.82337092605209	40.0	38.0	41.0	35.0	41.0
38	38.746421475658394	40.0	38.0	41.0	35.0	41.0
39	38.681881110523925	40.0	38.0	41.0	35.0	41.0
40	38.60678490485182	40.0	38.0	41.0	35.0	41.0
41	38.63795080880311	40.0	38.0	41.0	35.0	41.0
42	38.590809683435516	40.0	38.0	41.0	35.0	41.0
43	38.57030619993339	40.0	38.0	41.0	34.0	41.0
44	38.505946190155726	40.0	38.0	41.0	34.0	41.0
45	38.3468680593293	40.0	38.0	41.0	34.0	41.0
46	38.21534401919214	40.0	38.0	41.0	34.0	41.0
47	38.139045398336194	40.0	38.0	41.0	33.0	41.0
48	38.14391250149143	40.0	38.0	41.0	33.0	41.0
49	38.04561495640051	40.0	37.0	41.0	33.0	41.0
50	37.96659647156822	40.0	37.0	41.0	33.0	41.0
51	36.99360825044784	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	11.0
11	16.0
12	8.0
13	13.0
14	8.0
15	20.0
16	38.0
17	77.0
18	202.0
19	467.0
20	1109.0
21	1778.0
22	3075.0
23	5063.0
24	7922.0
25	12067.0
26	17847.0
27	25304.0
28	34969.0
29	47863.0
30	63225.0
31	81986.0
32	105885.0
33	137315.0
34	184066.0
35	258490.0
36	391089.0
37	664971.0
38	1436927.0
39	3740941.0
40	1870.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.142520115970804	25.457463155340825	29.901228727700158	21.498788000988213
2	18.50938719872835	33.543300372918964	28.91504695191142	19.03226547644127
3	18.244543039321343	28.66594063139047	34.87103692288016	18.21847940640803
4	17.867443934121987	27.628973458280054	29.828436793821577	24.675145813776382
5	23.81115645294303	28.278751038462058	30.172620700365666	17.737471808229245
6	23.877637402960374	28.868110266192325	28.478401511718392	18.775850819128905
7	18.657505592677044	34.34818632826114	29.198895555285493	17.795412523776317
8	18.014025916358854	34.62896210821155	29.03303229814249	18.3239796772871
9	23.627050036915406	28.279443115809734	29.407667607984134	18.685839239290726
10	18.19222199183736	34.19073873166583	28.763509695865224	18.85352958063158
11	23.870633580201936	28.450275488309014	28.696807281096625	18.98228365039242
12	18.15500207207958	28.536286861077652	28.633911291740223	24.674799775102546
13	18.080714489580497	34.43476520445488	28.60750162015307	18.877018685811557
14	18.057031602743173	28.776160869780664	28.689665042868658	24.47714248460751
15	17.989083448748765	34.487902903209104	28.78041022469537	18.74260342334676
16	18.043023957226296	28.652071401343125	28.84073168631843	24.46417295511214
17	23.820928585092155	28.617010762910077	28.81100004346246	18.751060608535308
18	18.203004556914088	28.521490247384435	34.47713417967934	18.798371016022145
19	18.173522011377266	28.487807322278297	28.762992793216274	24.575677873128164
20	18.25466252896346	28.51059294468083	34.34984815810382	18.884896368251894
21	23.936328591047282	28.50040689891258	28.750277022095183	18.812987487944955
22	18.26201302521299	34.24284201565517	28.676646665144062	18.81849829398778
23	18.240671238164946	34.27083144453707	28.725708580618576	18.7627887366794
24	18.245401215232455	28.615543558933016	28.721749748706713	24.41730547712781
25	18.253318580089818	28.534681241631056	34.3893095642598	18.822690614019326
26	18.240247726562224	28.551730136948546	28.765662456768563	24.44235967972067
27	18.287773995653442	28.587374483086204	28.66839625784401	24.45645526341635
28	18.36143747068998	34.24723559332483	28.593264566343336	18.79806236964185
29	23.99984781088077	28.633365401754883	28.5964469972822	18.770339790082147
30	18.410064624794188	28.576390923958172	34.22202990384497	18.79151454740267
31	18.332981696616702	28.572089126340583	28.58692985705829	24.507999319984428
32	18.35138498339688	34.254960954161085	28.543046421833584	18.850607640608448
33	18.352746530061264	28.612176802464433	28.614295109203557	24.42078155827074
34	18.34929600937482	28.631245227149943	28.629888723816894	24.38957003965834
35	18.378273463920255	28.662505738304517	34.28478436988294	18.674436427892285
36	18.396401020733336	28.617508229018807	34.34250769606023	18.64358305418763
37	24.006352213369247	28.60971335990195	28.698301567882535	18.685632858846272
38	18.432235999946847	28.703098769970808	34.20869192574646	18.655973304335884
39	18.391913150225726	34.277360242617675	28.626432679630714	18.70429392752589
40	18.379299893818416	34.30109630513284	28.608495108295106	18.711108692753637
41	18.380787056263394	28.619786042957525	34.22158175384973	18.77784514692935
42	18.427669568677498	28.58271838495697	28.63798862834126	24.351623418024275
43	18.417479706309038	28.52611878315374	34.213654082547976	18.842747427989238
44	18.427999111112957	28.574104726677618	28.615890725045222	24.3820054371642
45	24.052958524432185	28.61718393355006	28.652549967153192	18.677307574864564
46	18.426495245259968	28.64005256386597	34.21799966303084	18.71545252784322
47	18.4687367925752	34.239763843457695	28.658663454639417	18.632835909327696
48	18.495177906451843	28.643037723058768	34.22979740825536	18.63198696223403
49	24.090685432276253	28.574990675436236	28.605823345827925	18.728500546459586
50	18.540391595095585	28.57334920822391	28.546141835288918	24.340117361391588
51	18.530489536379168	28.506494324622224	28.54948384196416	24.413532297034443
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.64130811695901
2	37.54165267516962
3	36.46302244572937
4	42.54258974789837
5	41.54862826117227
6	42.65348822208928
7	36.45291811645336
8	36.33800559364595
9	42.312889276206135
10	37.045751572468944
11	42.85291723059436
12	42.829801847182125
13	36.95773317539206
14	42.53417408735068
15	36.73168687209552
16	42.50719691233844
17	42.571989193627466
18	37.00137557293623
19	42.74919988450543
20	37.13955889721536
21	42.74931607899224
22	37.080511319200774
23	37.00345997484435
24	42.66270669236027
25	37.07600919410915
26	42.68260740628289
27	42.74422925906979
28	37.15949984033183
29	42.77018760096291
30	37.20157917219686
31	42.840981016601134
32	37.20199262400533
33	42.77352808833201
34	42.73886604903316
35	37.05270989181254
36	37.03998407492096
37	42.691985072215516
38	37.088209304282735
39	37.096207077751615
40	37.090408586572046
41	37.158632203192745
42	42.77929298670177
43	37.260227134298276
44	42.810004548277156
45	42.73026609929675
46	37.14194777310319
47	37.101572701902896
48	37.12716486868587
49	42.81918597873584
50	42.880508956487176
51	42.944021833413615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15.0
1	20.5
2	26.0
3	51.5
4	77.0
5	230.0
6	383.0
7	649.5
8	916.0
9	1940.0
10	2964.0
11	5322.5
12	7681.0
13	12642.0
14	17603.0
15	26111.0
16	34619.0
17	48127.5
18	61636.0
19	81759.0
20	101882.0
21	127678.0
22	153474.0
23	182895.5
24	212317.0
25	240906.0
26	297818.5
27	326142.0
28	349832.5
29	373523.0
30	391547.5
31	409572.0
32	418194.5
33	426817.0
34	436794.0
35	446771.0
36	450313.0
37	453855.0
38	451486.5
39	449118.0
40	443485.0
41	437852.0
42	428857.5
43	419863.0
44	403172.5
45	386482.0
46	364881.0
47	343280.0
48	329496.5
49	315713.0
50	481121.5
51	646530.0
52	435411.5
53	224293.0
54	199964.5
55	175636.0
56	157739.5
57	139843.0
58	125728.5
59	111614.0
60	97980.5
61	84347.0
62	72344.0
63	60341.0
64	51414.0
65	42487.0
66	36163.0
67	29839.0
68	25233.0
69	20627.0
70	17160.5
71	13694.0
72	11511.5
73	9329.0
74	7601.0
75	4726.0
76	3579.0
77	2843.5
78	2108.0
79	1682.5
80	1257.0
81	935.5
82	614.0
83	449.5
84	285.0
85	218.0
86	151.0
87	103.5
88	56.0
89	44.5
90	33.0
91	21.5
92	10.0
93	6.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0484177312431121
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	6.090280659510956E-4
20	8.304928172060394E-5
21	0.006547051708974278
22	0.0024361122638043822
23	9.689082867403793E-5
24	0.0
25	0.0
26	0.06994133675570195
27	0.07722199045320824
28	0.09039914315287739
29	0.6829834513232934
30	0.10280116922315426
31	0.017468032255233697
32	0.08032249697077745
33	0.025966742084642166
34	0.0023392214351303444
35	0.01713583512835128
36	0.011073237562747192
37	0.018229317337672565
38	0.0011350068501815873
39	0.010381160215075493
40	0.02897035777353734
41	0.010325794027261756
42	0.026755710260987907
43	0.009772132149124398
44	0.029219505618699154
45	0.0024914784516181184
46	0.019558105845202227
47	0.013052578777088254
48	0.00953682585091602
49	0.024471855013671297
50	0.03209854738501342
51	0.026783393354894772
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7224626.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.826161990718056
#Duplication Level	Relative count
1	100.0
2	15.085049520515643
3	2.9529948121364566
4	1.2268511240371012
5	0.8891683697531835
6	0.7156107530262538
7	0.6741078446785097
8	0.5829272127024053
9	0.4747681182204056
10++	3.1649111774878165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC	375812	5.2018194436639345	TruSeq Adapter, Index 12 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3046115	3.7252295	3.909003	3
TTTTT	3106060	3.6991026	4.0594277	2
GGAAG	740565	3.2836359	87.58208	5
GAGCA	734965	3.1083007	83.43193	9
CGGAA	731260	3.0926316	83.65235	4
TCCAG	743410	2.9829452	78.029594	25
CTCCA	752655	2.8805628	74.45761	24
TCACC	736515	2.8187919	73.981804	30
GAAGA	965180	2.7857265	57.619495	6
TCGGA	647855	2.7253993	82.784065	3
TCTCG	682720	2.7249308	76.914955	41
GCACA	673150	2.7153933	79.19421	11
AGAGC	641455	2.71283	83.068535	8
CACAC	704420	2.7102983	75.58095	12
CTGAA	972945	2.6642742	54.327663	19
ATCGG	626690	2.6363623	82.585556	2
AGCAC	651335	2.6273947	79.12508	10
CCAGT	653680	2.622902	77.570564	26
GTCTG	621760	2.601783	81.60211	17
GATCG	596410	2.5089803	82.27571	1
GTCAC	624860	2.507261	77.55444	29
CACGT	616240	2.4726732	78.33902	14
ATGCC	615730	2.4706268	76.94274	47
CGTCT	617920	2.4662955	77.807816	16
CTCGT	616025	2.458732	76.32963	42
ACACG	602390	2.4299574	78.72164	13
CCTTG	607785	2.4258437	76.50212	33
CACCT	633365	2.4240158	73.40961	31
CAGTC	596780	2.3945897	77.331566	27
ACGTC	595310	2.3886912	78.105774	15
ACTCC	610210	2.335397	74.28785	23
AAGAG	787765	2.2736666	57.14517	7
TCTGA	833220	2.2695842	53.64557	18
TGAAC	803465	2.2001767	53.84712	20
GAACT	737790	2.020335	53.612408	21
CTTGT	726555	1.9685711	52.11509	34
AACTC	753330	1.9676164	51.10258	22
ATCTC	732720	1.903659	50.07114	40
AGTCA	691960	1.8948361	52.970108	28
ACCTT	665095	1.7279646	49.917465	32
GTATG	601640	1.718141	54.681477	45
TATGC	612415	1.6681397	52.123642	46
TCGTA	608335	1.6570263	52.133205	43
CGTAT	589735	1.6063622	52.121616	44
AATCT	871320	1.5449079	34.26623	39
TTGTA	828555	1.5320667	35.735306	35
TGTAA	770855	1.4329567	35.750088	36
GTAAT	705600	1.311653	35.578503	37
TAATC	690170	1.223717	33.86762	38
>>END_MODULE