##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW216_GGCTAC_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7880897	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57664920630228	34.0	31.0	34.0	31.0	34.0
2	32.812108951557164	34.0	31.0	34.0	31.0	34.0
3	32.90741739170046	34.0	31.0	34.0	31.0	34.0
4	36.32365363993464	37.0	37.0	37.0	35.0	37.0
5	36.24337191565884	37.0	37.0	37.0	35.0	37.0
6	36.22551709532557	37.0	37.0	37.0	35.0	37.0
7	36.19821626903638	37.0	37.0	37.0	35.0	37.0
8	36.17901794681494	37.0	37.0	37.0	35.0	37.0
9	37.96419417738869	39.0	38.0	39.0	35.0	39.0
10	37.92821324780669	39.0	38.0	39.0	35.0	39.0
11	37.927389356820676	39.0	38.0	39.0	35.0	39.0
12	37.910918135334086	39.0	38.0	39.0	35.0	39.0
13	37.88215770362181	39.0	38.0	39.0	35.0	39.0
14	39.380457199224914	41.0	39.0	41.0	36.0	41.0
15	39.33238640220777	41.0	39.0	41.0	36.0	41.0
16	39.3167129325507	41.0	39.0	41.0	36.0	41.0
17	39.30586074148666	40.0	39.0	41.0	36.0	41.0
18	39.26633249996796	40.0	39.0	41.0	36.0	41.0
19	39.31301233857009	40.0	39.0	41.0	36.0	41.0
20	39.289400940019895	40.0	39.0	41.0	36.0	41.0
21	39.2434749496155	40.0	39.0	41.0	36.0	41.0
22	39.18284530301563	40.0	39.0	41.0	36.0	41.0
23	39.11813997822836	40.0	39.0	41.0	36.0	41.0
24	39.02637199800987	40.0	39.0	41.0	36.0	41.0
25	39.01668541030291	40.0	39.0	41.0	36.0	41.0
26	38.882649779587275	40.0	38.0	41.0	35.0	41.0
27	38.78779877468263	40.0	38.0	41.0	35.0	41.0
28	38.67296159307754	40.0	38.0	41.0	35.0	41.0
29	38.37080499846655	40.0	38.0	41.0	34.0	41.0
30	38.375608893251616	40.0	38.0	41.0	34.0	41.0
31	38.60894768704628	40.0	38.0	41.0	35.0	41.0
32	38.57713950582021	40.0	38.0	41.0	34.0	41.0
33	38.61250032832557	40.0	38.0	41.0	35.0	41.0
34	38.75305983570144	40.0	38.0	41.0	35.0	41.0
35	38.81369823764985	40.0	38.0	41.0	35.0	41.0
36	38.81213534449188	40.0	38.0	41.0	35.0	41.0
37	38.792150436682526	40.0	38.0	41.0	35.0	41.0
38	38.73842089295165	40.0	38.0	41.0	35.0	41.0
39	38.69487191622984	40.0	38.0	41.0	35.0	41.0
40	38.61643947383147	40.0	38.0	41.0	35.0	41.0
41	38.605169436930844	40.0	38.0	41.0	35.0	41.0
42	38.547992316103105	40.0	38.0	41.0	34.0	41.0
43	38.51317940077126	40.0	38.0	41.0	34.0	41.0
44	38.43424993373216	40.0	38.0	41.0	34.0	41.0
45	38.321588900349795	40.0	38.0	41.0	34.0	41.0
46	38.25181575143033	40.0	38.0	41.0	34.0	41.0
47	38.1818003712014	40.0	38.0	41.0	33.0	41.0
48	38.136535346166816	40.0	38.0	41.0	33.0	41.0
49	38.05030290333702	40.0	38.0	41.0	33.0	41.0
50	37.96020364179357	40.0	37.0	41.0	33.0	41.0
51	36.968308810532605	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	6.0
11	6.0
12	10.0
13	9.0
14	8.0
15	21.0
16	35.0
17	112.0
18	207.0
19	488.0
20	1154.0
21	1942.0
22	3403.0
23	5626.0
24	8821.0
25	13311.0
26	19455.0
27	27743.0
28	38719.0
29	52220.0
30	68672.0
31	89097.0
32	115422.0
33	150039.0
34	201796.0
35	282789.0
36	428691.0
37	736800.0
38	1637232.0
39	3995478.0
40	1580.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.12855043928333	27.243031345061517	31.659732173201654	21.9686860424535
2	19.15648688214045	30.98616819887381	29.774427961690147	20.082916957295595
3	19.095935399231838	29.866371810213987	31.734319075607765	19.30337371494641
4	19.015868371328796	28.837263067896966	31.030795098578245	21.116073462195992
5	20.598213629742908	29.653033150921782	31.29230086372148	18.456452355613834
6	20.66349807642455	30.264879746556765	29.525002039742432	19.546620137276253
7	19.6598052226796	31.486555400990525	30.27940854955978	18.574230826770098
8	19.05520399517974	31.928015300796343	29.984695904539798	19.03208479948412
9	20.19777697894034	29.9759151781834	30.41877085819038	19.40753698468588
10	18.90744162752032	31.50929392935855	30.050322951816273	19.53294149130486
11	20.346338747987698	29.946172370987718	30.061666838178446	19.645822042846138
12	18.85692707314916	30.028244246816065	30.058037809655424	21.05679087037935
13	18.807148475611342	31.627085089425734	30.12537785990605	19.440388575056875
14	18.782481232783528	30.301233476341586	30.158318272653478	20.757967018221404
15	18.706474656374773	31.711504413774215	30.26755710676082	19.31446382309019
16	18.7597427044155	30.176488793090435	30.32526627362342	20.738502228870647
17	20.264114605228315	30.096155298058076	30.329999237396454	19.309730859317156
18	18.879031155971205	29.947555462277965	31.750243658811932	19.423169722938898
19	18.915612582644904	29.912338191731735	30.244078846774876	20.92797037884849
20	18.98585540106648	29.884309991462892	31.61661477742102	19.513219830049607
21	20.464304431633458	29.841441857573038	30.134422281130757	19.559831429662744
22	19.097050917381832	31.26492255498505	30.113436796381233	19.52458973125189
23	19.06088803145634	31.351324190205883	30.1006155140814	19.48717226425638
24	19.058160511424017	29.90611347921436	30.08201985129358	20.953706158068048
25	19.024814053527155	29.935259907596812	31.554732919361843	19.48519311951419
26	19.057519198313816	29.944709414916332	30.078697944370635	20.919073442399217
27	19.08181687615218	29.92853504655184	30.034531806583786	20.955116270712196
28	19.18726863702689	31.3548321054302	29.92341232309349	19.534486934449415
29	20.655093929998852	29.932056402206474	29.891477804554768	19.521371863239906
30	19.22735532489294	29.86917705541805	31.287748746376437	19.61571887331257
31	19.227982613028196	29.84064226867722	29.86519906295842	21.066176055336168
32	19.22188499564981	31.320120751354747	29.838159607323156	19.61983464567229
33	19.232434825408678	29.885793905099106	29.859940088189816	21.021831181302396
34	20.66593162375159	29.914478615466432	29.851616330946452	19.567973429835526
35	20.627447685724608	29.98159686797755	29.905400464513782	19.48555498178406
36	19.21289196169904	29.92962827082348	29.898536943666702	20.958942823810776
37	19.1785599384729	29.95912788384476	31.347320341318024	19.514991836364317
38	19.23966281058264	31.377527616827393	29.890875194920103	19.49193437766986
39	19.2509451984755	29.945263322466708	29.862650063305107	20.941141415752686
40	19.281030985356313	31.272216393607415	29.84418602230759	19.602566598728682
41	19.280653890096076	29.880188083883695	31.24553860413718	19.593619421883044
42	19.33590274514821	29.83775796607777	29.801255158433097	21.025084130340915
43	19.28061764533819	29.812867086685596	31.249304420579328	19.657210847396886
44	19.293315739611565	29.868245092255346	29.860527953810895	20.977911214322198
45	20.654664862428536	29.875643089754416	29.906008397219335	19.563683650597714
46	19.25349321814939	29.917052686779943	31.292751841239475	19.53670225383119
47	19.311612742097104	31.331301834440456	29.865838330861223	19.49124709260122
48	19.334165556591497	29.891497528520272	31.244069711324617	19.53026720356361
49	20.7597810543502	29.876293194862875	29.82387559488582	19.540050155901113
50	19.413719629333134	29.849593492323173	29.75659295376539	20.980093924578302
51	19.403878846525345	29.782424435194795	29.754831169576228	21.058865548703636
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.09723648173683
2	39.239403839436044
3	38.39930911417825
4	40.131941833524785
5	39.05466598535675
6	40.2101182137008
7	38.2340360494497
8	38.087288794663856
9	39.60531396362622
10	38.440383118825174
11	39.992160790833836
12	39.91371794352851
13	38.24753705066822
14	39.540448251004925
15	38.02093847946497
16	39.498244933286145
17	39.57384546454547
18	38.30220087891011
19	39.84358296149339
20	38.49907523111609
21	40.02413586129621
22	38.62164064863373
23	38.548060295712716
24	40.011866669492065
25	38.510007173041345
26	39.97659264071303
27	40.03693314686437
28	38.72175557147631
29	40.176465793238755
30	38.843074198205514
31	40.29415866836437
32	38.8417196413221
33	40.25426600671108
34	40.233905053587115
35	40.11300266750867
36	40.171834785509816
37	38.69355177483722
38	38.7315971882525
39	40.19208661422818
40	38.883597584084995
41	38.874273311979124
42	40.360986875489125
43	38.93782849273507
44	40.271226953933756
45	40.21834851302625
46	38.79019547198058
47	38.802859834698324
48	38.86443276015511
49	40.299831210251305
50	40.393813553911436
51	40.46274439522898
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	28.0
2	27.0
3	57.0
4	87.0
5	220.0
6	353.0
7	727.0
8	1101.0
9	2174.5
10	3248.0
11	5648.0
12	8048.0
13	12838.0
14	17628.0
15	26102.5
16	34577.0
17	48354.5
18	62132.0
19	81677.5
20	101223.0
21	126601.0
22	151979.0
23	181795.5
24	211612.0
25	242653.0
26	304616.5
27	335539.0
28	365672.5
29	395806.0
30	422246.5
31	448687.0
32	468238.0
33	487789.0
34	506487.0
35	525185.0
36	540103.5
37	555022.0
38	561835.0
39	568648.0
40	567278.0
41	565908.0
42	557993.0
43	550078.0
44	531892.5
45	513707.0
46	480850.0
47	447993.0
48	413595.5
49	379198.0
50	344787.5
51	310377.0
52	278413.5
53	246450.0
54	263541.5
55	280633.0
56	206362.5
57	132092.0
58	112723.0
59	93354.0
60	79176.5
61	64999.0
62	53864.0
63	42729.0
64	34976.5
65	27224.0
66	22260.0
67	17296.0
68	13990.5
69	10685.0
70	8549.5
71	6414.0
72	5127.5
73	3841.0
74	3151.0
75	1908.5
76	1356.0
77	1069.0
78	782.0
79	626.0
80	470.0
81	345.0
82	220.0
83	162.5
84	105.0
85	81.0
86	57.0
87	40.5
88	24.0
89	19.0
90	14.0
91	11.0
92	8.0
93	5.5
94	3.0
95	3.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05189764566140123
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.329342586256361E-4
20	1.1420019827692203E-4
21	0.0064332778362666074
22	0.002651982382208523
23	6.344455459829002E-5
24	0.0
25	0.0
26	0.07100714550640619
27	0.07775764611566424
28	0.09105562475946583
29	0.6866096587736142
30	0.10302126775670332
31	0.015277448747268236
32	0.0829093439490454
33	0.02516211035368182
34	0.0022586261436991248
35	0.015988027758769084
36	0.01124237507481699
37	0.01829740954614684
38	0.0013196467356444324
39	0.01022726220124435
40	0.0271923361008271
41	0.01010037309204777
42	0.026024956296218565
43	0.00984659487365461
44	0.029552473531883488
45	0.0024489598074939945
46	0.019769323212827167
47	0.01270159983057766
48	0.010151128735726402
49	0.02365212995424252
50	0.031062453931322793
51	0.027446114319220262
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7880897.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	13.317278983485565
#Duplication Level	Relative count
1	100.0
2	7.605958282984562
3	1.110119820664265
4	0.4963353623417543
5	0.391308766897424
6	0.3416187647517194
7	0.3116918316412382
8	0.3009633084506883
9	0.3026572857965646
10++	2.071169634891416
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC	99460	1.262039080069185	TruSeq Adapter, Index 11 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3187580	3.4068162	3.6683125	3
TTTTT	3208630	3.3656788	3.6574876	1
GAAGA	830245	2.1481683	15.799152	6
CTCCA	573545	2.11968	21.216581	24
GAGCA	538325	2.1088903	22.805517	9
GGAAG	516170	2.0779831	23.434853	5
TCCAG	523515	1.9882536	21.60454	25
CTGAA	784060	1.9667292	15.09735	19
CGGAA	497660	1.949585	22.720907	4
CGGCT	289840	1.7127272	31.458538	33
TCTCG	450385	1.704118	20.838692	41
TCGGA	429780	1.6773692	22.404139	3
CACAC	436185	1.6180817	21.063238	12
ACGGC	272400	1.6157117	31.428701	32
AGAGC	411385	1.6116023	22.3236	8
TCTGA	629670	1.573553	14.6518955	18
AGCAC	412545	1.5726817	21.739334	10
GCACA	410720	1.5657245	21.64447	11
CACGG	261020	1.5482124	31.52548	31
AAGAG	569465	1.4734284	15.129159	7
CCAGT	381630	1.4493896	21.050577	26
ATCGG	370595	1.4463787	22.055964	2
CTCGT	371820	1.4068522	20.508902	42
TGAAC	559460	1.4033445	14.537751	20
CGTCT	363850	1.3766961	21.174856	16
CACGT	360030	1.3673551	21.255043	14
GTCAC	356445	1.3537397	20.82163	29
GTCTG	345650	1.3439779	21.75509	17
ACTCC	356045	1.315854	20.493282	23
ACACG	336600	1.2831684	21.310654	13
GATCG	326655	1.2748872	21.75537	1
ATGCC	335435	1.273946	20.372213	47
CATCT	523585	1.273256	13.411323	39
AACTC	521355	1.2725912	14.01196	22
TCACG	331275	1.2581469	20.563227	30
ACGTC	330665	1.25583	21.127705	15
CAGTC	329445	1.2511966	20.828812	27
GAACT	494945	1.2415156	14.345161	21
ATCTC	507475	1.2340796	13.511457	40
CTACA	474925	1.159259	13.301231	36
ACATC	464745	1.1344103	13.299412	38
AGTCA	434355	1.0895323	13.991615	28
GCTAC	272140	1.0335584	19.985489	35
GGCTA	242065	0.94474477	20.560629	34
TCGTA	375600	0.938629	13.494111	43
TATGC	340485	0.85087615	13.357231	46
GTATG	326150	0.8375787	13.737426	45
CGTAT	328595	0.8211629	13.374365	44
TACAT	479955	0.7708709	8.811427	37
>>END_MODULE