##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW214_GATCAG_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9608336	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.584113732075984	34.0	31.0	34.0	31.0	34.0
2	32.80987602848194	34.0	31.0	34.0	31.0	34.0
3	32.91132408358742	34.0	31.0	34.0	31.0	34.0
4	36.3286001863382	37.0	37.0	37.0	35.0	37.0
5	36.249929956654306	37.0	37.0	37.0	35.0	37.0
6	36.22772330193282	37.0	37.0	37.0	35.0	37.0
7	36.19290312079011	37.0	37.0	37.0	35.0	37.0
8	36.18594780615499	37.0	37.0	37.0	35.0	37.0
9	37.969342766531064	39.0	38.0	39.0	35.0	39.0
10	37.92738825952797	39.0	38.0	39.0	35.0	39.0
11	37.93103863145502	39.0	38.0	39.0	35.0	39.0
12	37.92322250179428	39.0	38.0	39.0	35.0	39.0
13	37.88781470589704	39.0	38.0	39.0	35.0	39.0
14	39.40643031217893	41.0	39.0	41.0	36.0	41.0
15	39.31693541941081	41.0	39.0	41.0	36.0	41.0
16	39.33881329712033	41.0	39.0	41.0	36.0	41.0
17	39.32438239045762	41.0	39.0	41.0	36.0	41.0
18	39.281650329463915	40.0	39.0	41.0	36.0	41.0
19	39.33372958647574	40.0	39.0	41.0	36.0	41.0
20	39.31430093618708	40.0	39.0	41.0	36.0	41.0
21	39.266640342302765	40.0	39.0	41.0	36.0	41.0
22	39.160012097828385	40.0	39.0	41.0	36.0	41.0
23	39.10228524481242	40.0	39.0	41.0	36.0	41.0
24	39.05453181487408	40.0	39.0	41.0	36.0	41.0
25	39.03455863741651	40.0	39.0	41.0	36.0	41.0
26	38.93339023531234	40.0	38.0	41.0	36.0	41.0
27	38.848074942424994	40.0	38.0	41.0	35.0	41.0
28	38.61553280401518	40.0	38.0	41.0	35.0	41.0
29	38.37847323407508	40.0	38.0	41.0	34.0	41.0
30	38.32875380294777	40.0	38.0	41.0	34.0	41.0
31	38.607364896481556	40.0	38.0	41.0	35.0	41.0
32	38.559818266138905	40.0	38.0	41.0	34.0	41.0
33	38.642260532937236	40.0	38.0	41.0	35.0	41.0
34	38.76682892854704	40.0	38.0	41.0	35.0	41.0
35	38.73750158195966	40.0	38.0	41.0	35.0	41.0
36	38.809199948877726	40.0	38.0	41.0	35.0	41.0
37	38.75678691919183	40.0	38.0	41.0	35.0	41.0
38	38.72412018064314	40.0	38.0	41.0	35.0	41.0
39	38.71131140709484	40.0	38.0	41.0	35.0	41.0
40	38.585900722039696	40.0	38.0	41.0	35.0	41.0
41	38.596221864014744	40.0	38.0	41.0	35.0	41.0
42	38.574862286248106	40.0	38.0	41.0	34.0	41.0
43	38.5505190492922	40.0	38.0	41.0	34.0	41.0
44	38.49513162320718	40.0	38.0	41.0	34.0	41.0
45	38.37809377190806	40.0	38.0	41.0	34.0	41.0
46	38.263693005740016	40.0	38.0	41.0	34.0	41.0
47	38.19586169759259	40.0	38.0	41.0	33.0	41.0
48	38.17782309028327	40.0	38.0	41.0	33.0	41.0
49	38.09540934038943	40.0	38.0	41.0	33.0	41.0
50	38.006255089330764	40.0	37.0	41.0	33.0	41.0
51	36.99970504778351	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	7.0
11	8.0
12	7.0
13	16.0
14	9.0
15	16.0
16	56.0
17	115.0
18	277.0
19	613.0
20	1400.0
21	2438.0
22	4107.0
23	6789.0
24	10825.0
25	16275.0
26	24224.0
27	34228.0
28	47400.0
29	63577.0
30	84620.0
31	109091.0
32	141133.0
33	183388.0
34	244961.0
35	344392.0
36	520054.0
37	884622.0
38	1950506.0
39	4931046.0
40	2129.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.39780992749705	27.010845610228408	31.116729137973024	20.47461532430152
2	18.379498801873705	32.59377065914431	30.090662941012887	18.936067597969096
3	18.371131067856076	28.98484191227284	33.7028388682494	18.941188151621674
4	18.55831228216832	28.184068500518716	29.9572995781996	23.300319639113372
5	22.80284536261013	28.844807259030077	29.94768292865695	18.40466444970284
6	22.907202662354855	29.302222570068327	28.72465117789386	19.06592358968296
7	19.027946150093005	33.34228736380576	29.210906029930676	18.418860456170556
8	18.504712990886247	33.53177907183928	29.188893893802216	18.77461404347225
9	22.38588450695313	29.026763843395987	29.539339590122577	19.04801205952831
10	18.43362888225391	33.184299549890845	29.231377836911616	19.15069373094363
11	22.514803812023228	29.005740432058165	29.26496325690525	19.214492499013357
12	18.40593418048661	29.01564849522331	29.235384774221053	23.343032550069022
13	18.358017454843377	33.212681155196904	29.302201754809577	19.127099635150145
14	18.36820652400166	29.23563455732606	29.334715188977572	23.061443729694712
15	18.339679211884345	33.291623024007485	29.333351789529427	19.03534597457874
16	18.39619455399921	29.18641991562534	29.35225509934864	23.065130431026812
17	22.515886205478246	29.097077787454563	29.3479432859134	19.039092721153796
18	18.52468523165718	28.96977166493761	33.35175830653716	19.153784796868052
19	18.53011265987608	28.93500109957005	29.288206104284203	23.24668013626967
20	18.602079508309927	28.9029132953295	33.29380165895652	19.20120553740406
21	22.68194260853274	28.889692642359776	29.176556303797994	19.25180844530949
22	18.669086990419835	32.96508428686228	29.16162967402127	19.204199048696612
23	18.618151556570737	32.99251805698241	29.17685096884132	19.21247941760553
24	18.659590865639053	28.909066971540852	29.168945504085777	23.262396658734318
25	18.634808358075738	28.952442962027973	33.1652952186518	19.247453461244486
26	18.675939179933945	28.94579302047332	29.152467596959163	23.22580020263357
27	18.70518119172409	28.965594820474788	29.07701169059503	23.25221229720609
28	18.811887376433585	32.955074827369536	29.01163745185264	19.22140034434424
29	22.842395484678214	28.951556281189024	29.036270961224485	19.169777272908277
30	18.884168596566695	28.844807283129644	32.95341329504129	19.317610825262378
31	18.80828335968639	28.86806436737579	28.989916443992907	23.333735828944917
32	18.823992378715985	32.91320164966717	28.944473829543142	19.3183321420737
33	18.882097888266006	28.88514811286872	28.951055866997716	23.28169813186756
34	22.84646467337794	28.96268443277282	28.959260241454405	19.231590652394832
35	18.865044676366853	32.925419056607765	29.003231787792693	19.20630447923269
36	18.820690982478904	28.99681999546383	32.963004174256874	19.219484847800388
37	18.809094710491543	29.016790390097047	29.018226916401556	23.155887983009848
38	18.868308829666923	32.96796223068118	28.94811339704682	19.21561554260507
39	22.916598576040244	28.986101601553987	28.89475433977559	19.202545482630175
40	18.95976646106076	32.87604419633436	28.91356108883647	19.250628253768404
41	18.91717334640115	28.894526026930723	32.891094059757364	19.297206566910766
42	18.92396880586805	28.84222532243864	28.946818587681733	23.286987284011577
43	18.91228719894656	28.82771791586663	32.908545703728656	19.351449181458147
44	18.894859442141072	28.87719763988767	28.960868671252594	23.267074246718668
45	22.892949260092895	28.879670044997457	28.95132834823292	19.27605234667673
46	18.91356975499383	28.98501965137597	32.90622802258316	19.19518257104704
47	18.935931692114885	32.9630200195897	28.957093146832186	19.14395514146323
48	18.969609101666915	28.922538010611216	32.91283380708357	19.195019080638296
49	22.952659717719087	28.891918488547162	28.909261882436983	19.246159911296765
50	19.047140639012508	28.859172757254413	28.863493307519693	23.230193296213386
51	19.060675760281846	28.78313886921888	28.811101392358758	23.34508397814051
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.87242525179857
2	37.3155663998428
3	37.31231921947775
4	41.858631921281685
5	41.20750981231298
6	41.973126252037815
7	37.446806606263564
8	37.2793270343585
9	41.43389656648144
10	37.58432261319754
11	41.729296311036585
12	41.74896673055564
13	37.48511708999352
14	41.42965025369637
15	37.37502518646309
16	41.461324985026025
17	41.55497892663204
18	37.67847002852523
19	41.77679279614575
20	37.803285045713984
21	41.933751053842236
22	37.87328603911645
23	37.83063097417627
24	41.92198752437337
25	37.88226181932022
26	41.90173938256751
27	41.95739348893019
28	38.03328772077783
29	42.01217275758649
30	38.20177942182907
31	42.1420191886313
32	38.142324520789685
33	42.16379602013357
34	42.07805532577277
35	38.07134915559954
36	38.040175830279296
37	41.9649826935014
38	38.083924372272
39	42.11914405867042
40	38.21039471482916
41	38.214379913311916
42	42.21095608987962
43	38.26373638040471
44	42.16193368885974
45	42.169001606769626
46	38.10875232604087
47	38.07988683357812
48	38.16462818230521
49	42.19881962901585
50	42.27733393522589
51	42.40575973842236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	36.0
2	38.0
3	96.5
4	155.0
5	319.0
6	483.0
7	935.5
8	1388.0
9	2681.0
10	3974.0
11	6806.5
12	9639.0
13	15499.5
14	21360.0
15	31565.0
16	41770.0
17	57219.0
18	72668.0
19	96217.0
20	119766.0
21	148961.0
22	178156.0
23	212428.5
24	246701.0
25	282058.5
26	354280.5
27	391145.0
28	425148.5
29	459152.0
30	491192.5
31	523233.0
32	545912.0
33	568591.0
34	588858.5
35	609126.0
36	625913.5
37	642701.0
38	651132.0
39	659563.0
40	660997.5
41	662432.0
42	654598.0
43	646764.0
44	627378.5
45	607993.0
46	572669.0
47	537345.0
48	499864.5
49	462384.0
50	431432.5
51	400481.0
52	530378.5
53	660276.0
54	448840.5
55	237405.0
56	201406.5
57	165408.0
58	143162.0
59	120916.0
60	103014.0
61	85112.0
62	71135.0
63	57158.0
64	46836.5
65	36515.0
66	30233.5
67	23952.0
68	19335.0
69	14718.0
70	11982.0
71	9246.0
72	7362.5
73	5479.0
74	4415.5
75	2683.5
76	2015.0
77	1579.5
78	1144.0
79	894.0
80	644.0
81	458.5
82	273.0
83	201.5
84	130.0
85	101.5
86	73.0
87	57.0
88	41.0
89	25.0
90	9.0
91	8.0
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049196864056377715
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0407629375159237E-5
17	0.0
18	0.0
19	3.6426702813057327E-4
20	7.285340562611466E-5
21	0.006619252282601274
22	0.0026539454906656054
23	3.122288812547771E-5
24	1.0407629375159237E-5
25	0.0
26	0.07043883561107772
27	0.07759928462118727
28	0.0899219178013758
29	0.6839477720179644
30	0.1022549586109395
31	0.016714652776505735
32	0.08174152111250065
33	0.025873366626645865
34	0.002372939497536306
35	0.016433646783376436
36	0.011625322012052868
37	0.019035554127166242
38	0.001186469748768153
39	0.010844749808915925
40	0.02854812737606179
41	0.010594966703912103
42	0.026591493053531853
43	0.009856025018275797
44	0.029776227642330576
45	0.0024770157912878984
46	0.019607973742800003
47	0.01296790620144841
48	0.009918470794526754
49	0.02469730450725287
50	0.03203468321674013
51	0.027288804221667518
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9608336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	17.38701372997711
#Duplication Level	Relative count
1	100.0
2	9.381437944705088
3	1.3595785075798232
4	0.579956027214653
5	0.3895227048456624
6	0.33412392015650155
7	0.2868041249011766
8	0.28507291287964037
9	0.2787251354673407
10++	2.518913491335284
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC	350543	3.648321624056444	TruSeq Adapter, Index 9 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3778640	3.4609442	3.961253	1
AAAAA	3717520	3.3750203	3.5858076	12
GAGCA	900310	2.8191345	59.341103	9
GGAAG	867685	2.817027	61.56899	5
CTCCA	934990	2.72827	54.179848	24
CGGAA	850190	2.662194	59.380062	4
TCCAG	872320	2.6391344	56.079666	25
GAAGA	1242260	2.6373806	41.01144	6
TCTCG	801005	2.4276612	55.748413	41
TCGGA	769215	2.4128962	59.23172	3
CTGAA	1171610	2.4032855	39.01232	19
AGAGC	751760	2.3539805	58.894142	8
CACAC	796170	2.3190978	54.603786	12
AGCAC	761680	2.3003345	56.68969	10
GCACA	757865	2.288813	56.618793	11
ATCGG	702755	2.2044227	58.89003	2
CTCGT	705245	2.137435	55.282764	42
CCAGT	703790	2.1292603	55.39671	26
CGTCT	699990	2.1215081	56.32978	16
CACGA	701695	2.1191752	54.87398	31
GTCTG	673220	2.1155105	58.349964	17
CACGT	690175	2.0880692	56.247997	14
TCAGA	997945	2.0470521	37.675556	36
GTCAC	676475	2.046621	55.446053	29
TCTGA	995495	2.045637	38.758034	18
GATCG	650170	2.039472	58.546276	1
ACACG	664105	2.00565	56.17495	13
ATGCC	660215	1.9974277	55.19769	47
AAGAG	940080	1.9958372	40.361004	7
ACTCC	683850	1.9954518	53.77838	23
ACGTC	655315	1.9826031	56.115982	15
TCACG	655195	1.9822401	55.208588	30
CAGTC	646935	1.9572501	55.248676	27
CGATC	641510	1.9408371	54.517937	33
TGAAC	911065	1.868838	38.46034	20
ATCAG	847685	1.7388285	37.312572	35
GATCA	840075	1.7232183	37.337425	34
AACTC	869830	1.7208831	36.907185	22
GAACT	836820	1.7165414	38.26045	21
ATCTC	862265	1.7089329	36.428658	40
CAGAT	826980	1.6963571	37.28585	37
ACGAT	809580	1.660665	37.489468	32
AGATC	787980	1.6163577	37.37183	38
GATCT	775565	1.593704	37.489544	39
AGTCA	762625	1.5643477	37.659935	28
TCGTA	723535	1.4867878	37.580887	43
GTATG	649870	1.3845901	38.843235	45
TATGC	667525	1.3716933	37.44075	46
CGTAT	663070	1.3625387	37.47037	44
>>END_MODULE