FastQCFastQC Report
Wed 23 Jul 2014
LW213_ACTTGA_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW213_ACTTGA_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5394063
Filtered Sequences 0
Sequence length 51
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC 143878 2.6673399995513587 TruSeq Adapter, Index 8 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 2047710 3.3384767 3.9346588 1
AAAAA 2040800 3.3379745 3.718208 3
CACAC 532360 2.7500305 39.98091 12
GAGCA 452520 2.5271187 43.968365 9
GGAAG 434330 2.5219452 45.639664 5
CTCCA 471135 2.4321868 39.82101 24
GAAGA 637670 2.427173 30.609568 6
TCCAG 440995 2.367081 41.241577 25
CGGAA 414820 2.3165812 43.84119 4
TTGAA 895505 2.2315192 19.966694 36
CTGAA 605530 2.215297 29.10959 19
TCGGA 372465 2.0787048 43.497112 3
TCTCG 386440 2.0729125 40.618015 41
AGAGC 362325 2.0234208 43.434757 8
AGCAC 373720 2.0072722 41.809635 10
GCACA 360935 1.9386035 41.764244 11
ATCGG 335510 1.8724611 43.18921 2
CCAGT 345510 1.8545566 40.63643 26
TCTGA 504155 1.8432314 28.706198 18
CTCGT 334520 1.7944069 40.21219 42
GTCTG 318970 1.7790029 42.69394 17
CGTCT 330380 1.7721999 41.041767 16
AAGAG 463560 1.7644554 29.945618 7
CACGT 328290 1.7621268 41.260937 14
GTCAC 322685 1.7320412 40.466427 29
GATCG 310145 1.7309006 42.821503 1
ATGCC 315870 1.6954612 40.153305 47
ACTCC 325955 1.6827097 39.336662 23
ACGTC 310795 1.6682205 41.096012 15
ACACG 310460 1.6674993 41.264904 13
TGAAC 455520 1.6664938 28.52624 20
CTTGA 455540 1.665491 27.794495 35
CAGTC 304570 1.6348073 40.382698 27
TCACA 463465 1.6307423 26.903728 30
CACTT 454145 1.5969172 26.819246 33
GAATC 431945 1.5802461 27.609095 38
ACTTG 422940 1.5463026 27.693056 34
TGAAT 615270 1.5331982 19.310072 37
AACTC 435525 1.532433 27.290232 22
GAACT 415130 1.5187294 28.369053 21
ATCTC 427530 1.5033305 26.737421 40
AGTCA 374350 1.3695381 27.700436 28
ACACT 373025 1.3125212 26.478117 32
TCGTA 343435 1.2556261 27.44391 43
AATCT 517920 1.2412733 18.337858 39
GTATG 303220 1.1526607 28.377289 45
TATGC 314695 1.1505504 27.292437 46
CGTAT 309895 1.133001 27.299677 44
Produced by FastQC (version 0.10.1)