##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW213_ACTTGA_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5394063	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60260512344776	34.0	31.0	34.0	31.0	34.0
2	32.83509554856886	34.0	31.0	34.0	31.0	34.0
3	32.93488229559054	34.0	31.0	34.0	31.0	34.0
4	36.339781533882714	37.0	37.0	37.0	35.0	37.0
5	36.262502495799545	37.0	37.0	37.0	35.0	37.0
6	36.242173293118746	37.0	37.0	37.0	35.0	37.0
7	36.20420043295749	37.0	37.0	37.0	35.0	37.0
8	36.18938247476902	37.0	37.0	37.0	35.0	37.0
9	37.98674672505679	39.0	38.0	39.0	35.0	39.0
10	37.93906671093756	39.0	38.0	39.0	35.0	39.0
11	37.943706441693394	39.0	38.0	39.0	35.0	39.0
12	37.92934342813571	39.0	38.0	39.0	35.0	39.0
13	37.902132770788924	39.0	38.0	39.0	35.0	39.0
14	39.41703183666932	41.0	39.0	41.0	36.0	41.0
15	39.35365030033947	41.0	39.0	41.0	36.0	41.0
16	39.350844252282556	41.0	39.0	41.0	36.0	41.0
17	39.339109684110106	41.0	39.0	41.0	36.0	41.0
18	39.29800745745832	40.0	39.0	41.0	36.0	41.0
19	39.349270855753815	40.0	39.0	41.0	36.0	41.0
20	39.324793759360986	40.0	39.0	41.0	36.0	41.0
21	39.27745856880055	40.0	39.0	41.0	36.0	41.0
22	39.19421853248655	40.0	39.0	41.0	36.0	41.0
23	39.15014359305778	40.0	39.0	41.0	36.0	41.0
24	39.0748040947983	40.0	39.0	41.0	36.0	41.0
25	39.060007456345986	40.0	39.0	41.0	36.0	41.0
26	38.94370792480547	40.0	38.0	41.0	36.0	41.0
27	38.84458950516522	40.0	38.0	41.0	35.0	41.0
28	38.6858036326235	40.0	38.0	41.0	35.0	41.0
29	38.41563048114195	40.0	38.0	41.0	34.0	41.0
30	38.38128364462929	40.0	38.0	41.0	34.0	41.0
31	38.641554612914234	40.0	38.0	41.0	35.0	41.0
32	38.597828390213465	40.0	38.0	41.0	35.0	41.0
33	38.665023378481116	40.0	38.0	41.0	35.0	41.0
34	38.7968601775693	40.0	38.0	41.0	35.0	41.0
35	38.87505652047445	40.0	38.0	41.0	35.0	41.0
36	38.875385585967386	40.0	38.0	41.0	35.0	41.0
37	38.84993445571548	40.0	38.0	41.0	35.0	41.0
38	38.781303073397545	40.0	38.0	41.0	35.0	41.0
39	38.694666710418474	40.0	38.0	41.0	35.0	41.0
40	38.64240221146842	40.0	38.0	41.0	35.0	41.0
41	38.65151927220724	40.0	38.0	41.0	35.0	41.0
42	38.60357897191783	40.0	38.0	41.0	35.0	41.0
43	38.57144568018579	40.0	38.0	41.0	34.0	41.0
44	38.50163837537678	40.0	38.0	41.0	34.0	41.0
45	38.375111117537934	40.0	38.0	41.0	34.0	41.0
46	38.27992183257778	40.0	38.0	41.0	34.0	41.0
47	38.208870567510985	40.0	38.0	41.0	34.0	41.0
48	38.18030082333113	40.0	38.0	41.0	33.0	41.0
49	38.093172623308256	40.0	38.0	41.0	33.0	41.0
50	38.00769846403351	40.0	37.0	41.0	33.0	41.0
51	37.00299366173513	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	10.0
11	4.0
12	5.0
13	6.0
14	10.0
15	12.0
16	29.0
17	64.0
18	130.0
19	327.0
20	793.0
21	1257.0
22	2287.0
23	3784.0
24	5873.0
25	8880.0
26	12987.0
27	18598.0
28	25989.0
29	34727.0
30	45975.0
31	59632.0
32	77680.0
33	100736.0
34	135511.0
35	189981.0
36	289075.0
37	493587.0
38	1093939.0
39	2791006.0
40	1163.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.474776589469936	26.688237978120792	31.666629570688244	22.17035586172103
2	18.7693024719956	31.9208173875611	29.372237587881344	19.937642552561954
3	18.970431009055698	29.488216952601405	32.26918187644453	19.27217016189837
4	18.66971149576859	28.36646513027378	30.30860040010656	22.655222973851068
5	21.911683271033354	29.055519003022397	30.827318850373086	18.20547887557116
6	21.951912686225576	29.674477291051293	28.925394456831523	19.448215565891612
7	19.519683029286085	32.31389770568123	29.802284474615888	18.3641347904168
8	18.834448170145585	32.773755145240244	29.51786065531678	18.873936029297397
9	21.53615929216993	29.143226543701843	29.99241944337691	19.328194720751316
10	18.81468570166867	32.32934061022276	29.38647546385721	19.469498224251367
11	21.782800831210167	29.249361751985468	29.417973056673606	19.54986436013076
12	18.753080933611642	29.34980551765895	29.35451439851555	22.542599150213853
13	18.717931177296222	32.49652441953311	29.37663130742077	19.408913095749902
14	18.685933034152548	29.658292830469353	29.411095124398805	22.244679010979294
15	18.59572274183672	32.54133294327486	29.58567595521224	19.277268359676185
16	18.664542602587808	29.48723985745807	29.620775586190888	22.22744195376323
17	21.721566841173342	29.410112562645264	29.574738003616197	19.293582592565194
18	18.785690860488653	29.2689759092543	32.52179294161006	19.423540288646983
19	18.826396847778973	29.222486011407412	29.485554235991664	22.465562904821955
20	18.940163023891486	29.168328990281157	32.39753165336066	19.4939763324667
21	21.94419554419421	29.13239769087312	29.35608346510678	19.56732329982589
22	18.93934028757532	32.143729817576286	29.36541954783955	19.551510347008836
23	18.97180545181484	32.180861270097814	29.37883303791562	19.46850024017173
24	18.987171636667945	29.178209449908167	29.387884420333986	22.446734493089902
25	18.970134386639533	29.20509085637302	32.283308519014334	19.541466237973122
26	19.005084387927333	29.2288672126178	29.315857999274986	22.45019040017988
27	19.08587916485619	29.205116511890157	29.23366980078747	22.475334522466188
28	19.10591364858418	32.101786328277	29.212513668512884	19.579786354625934
29	22.15942657398263	29.179437911544248	29.150842020206397	19.51029349426673
30	19.21964371246761	29.08002634493041	32.0108534580876	19.689476484514373
31	19.148903425820855	29.08897566547256	29.135534640173848	22.626586268532733
32	19.203629144479283	32.00426739892758	29.094402285841515	19.697701170751618
33	19.19407717840785	29.1787416322065	29.083186529938622	22.54399465944703
34	19.168071481580565	32.0785939948635	29.096085261466136	19.657249262089795
35	19.16044321911066	29.234334703150033	29.147206128333586	22.45801594940572
36	19.1610173875365	29.122932380525235	32.07063145538644	19.64541877655183
37	19.200585936594614	29.143466389768356	32.07956499770539	19.576382675931633
38	22.189556278952324	29.178047373774955	29.064606450467068	19.56778989680565
39	19.277126527887862	32.05480696582445	29.057592739374893	19.610473766912797
40	19.28744379492442	32.001515429958744	29.041549892453062	19.669490882663773
41	19.260848138483578	29.139654243455	31.922537955830588	19.67695966223084
42	19.288154903830428	29.108425486336802	29.028019927820438	22.575399682012335
43	19.273315855634703	29.019679097587115	31.96516573530557	19.741839311472614
44	19.28580462325827	29.07281518649881	29.09925944937333	22.54212074086959
45	22.152326782142147	29.12488667817343	29.095038311583576	19.627748228100845
46	19.238656210429152	29.133488731817458	31.9698417416448	19.65801331610859
47	19.345861158334632	32.06252256237785	29.064662778320315	19.526953500967203
48	19.335543762397972	29.106800879499357	31.93775200365327	19.619903354449402
49	22.223862277853073	29.054858810062584	29.039004252173882	19.682274659910462
50	19.40786734661897	29.032336431370503	29.00987864449917	22.549917577511355
51	19.432270884935615	28.9856835478647	28.965173987275545	22.616871579924144
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.64513245119096
2	38.706945024557555
3	38.24260117095407
4	41.324934469619656
5	40.11716214660452
6	41.40012825211719
7	37.883817819702884
8	37.70838419944298
9	40.86435401292125
10	38.28418392592003
11	41.33266519134092
12	41.295680083825495
13	38.126844273046125
14	40.930612045131845
15	37.8729911015129
16	40.89198455635104
17	41.01514943373854
18	38.20923114913563
19	41.291959752600924
20	38.43413935635818
21	41.5115188440201
22	38.49085063458416
23	38.44030569198657
24	41.433906129757844
25	38.511600624612655
26	41.45527478810722
27	41.56121368732238
28	38.685700003210115
29	41.66972006824936
30	38.90912019698199
31	41.77548969435359
32	38.9013303152309
33	41.73807183785488
34	38.82532074367036
35	41.61845916851638
36	38.806436164088325
37	38.77696861252625
38	41.757346175757974
39	38.88760029480066
40	38.956934677588194
41	38.93780780071442
42	41.86355458584276
43	39.01515516710732
44	41.82792536412786
45	41.780075010242996
46	38.89666952653774
47	38.87281465930184
48	38.955447116847374
49	41.90613693776354
50	41.95778492413032
51	42.04914246485976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	17.5
2	24.0
3	47.0
4	70.0
5	142.5
6	215.0
7	440.5
8	666.0
9	1313.5
10	1961.0
11	3486.0
12	5011.0
13	8085.5
14	11160.0
15	16335.0
16	21510.0
17	30203.0
18	38896.0
19	51287.0
20	63678.0
21	80066.5
22	96455.0
23	115524.0
24	134593.0
25	154631.0
26	194549.5
27	214430.0
28	234436.5
29	254443.0
30	273297.5
31	292152.0
32	305667.0
33	319182.0
34	332101.5
35	345021.0
36	355851.0
37	366681.0
38	372358.0
39	378035.0
40	379048.5
41	380062.0
42	376040.0
43	372018.0
44	361587.0
45	351156.0
46	330062.5
47	308969.0
48	290482.0
49	271995.0
50	319925.5
51	367856.0
52	272246.0
53	176636.0
54	153519.0
55	130402.0
56	113079.0
57	95756.0
58	83298.5
59	70841.0
60	60651.5
61	50462.0
62	42489.0
63	34516.0
64	28853.5
65	23191.0
66	19177.5
67	15164.0
68	12614.5
69	10065.0
70	8214.0
71	6363.0
72	5170.5
73	3978.0
74	3242.0
75	1984.0
76	1462.0
77	1187.5
78	913.0
79	695.5
80	478.0
81	330.5
82	183.0
83	153.0
84	123.0
85	90.0
86	57.0
87	43.5
88	30.0
89	20.0
90	10.0
91	7.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.048998315370065194
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.8538901010240333E-5
17	0.0
18	0.0
19	4.634725252560083E-4
20	1.2977230707168235E-4
21	0.006229070739440752
22	0.0025398294384029255
23	1.11233406061442E-4
24	0.0
25	0.0
26	0.07113376317629215
27	0.07676958908340521
28	0.0899507477016861
29	0.6794507220253082
30	0.10289090060683385
31	0.01674062761224702
32	0.08088522510767858
33	0.025268522076957574
34	0.002113434715167398
35	0.016388388493052453
36	0.011160418408164681
37	0.01831643419811745
38	0.0012235674666758619
39	0.010900873794021316
40	0.02843867414970867
41	0.010326167862703866
42	0.025973000315346707
43	0.009695845228355694
44	0.02945831370527189
45	0.0024656738343619643
46	0.019391690456711387
47	0.012643530488983905
48	0.01001100654552978
49	0.02395226010523051
50	0.031961065341654334
51	0.026992639870909923
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5394063.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	13.826953552109982
#Duplication Level	Relative count
1	100.0
2	6.829958133737277
3	1.0145162462339927
4	0.5589621191371861
5	0.4494055437862976
6	0.4460517710714745
7	0.40245272577877395
8	0.3733866955836403
9	0.3130187867168242
10++	1.4046718053917486
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC	143878	2.6673399995513587	TruSeq Adapter, Index 8 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2047710	3.3384767	3.9346588	1
AAAAA	2040800	3.3379745	3.718208	3
CACAC	532360	2.7500305	39.98091	12
GAGCA	452520	2.5271187	43.968365	9
GGAAG	434330	2.5219452	45.639664	5
CTCCA	471135	2.4321868	39.82101	24
GAAGA	637670	2.427173	30.609568	6
TCCAG	440995	2.367081	41.241577	25
CGGAA	414820	2.3165812	43.84119	4
TTGAA	895505	2.2315192	19.966694	36
CTGAA	605530	2.215297	29.10959	19
TCGGA	372465	2.0787048	43.497112	3
TCTCG	386440	2.0729125	40.618015	41
AGAGC	362325	2.0234208	43.434757	8
AGCAC	373720	2.0072722	41.809635	10
GCACA	360935	1.9386035	41.764244	11
ATCGG	335510	1.8724611	43.18921	2
CCAGT	345510	1.8545566	40.63643	26
TCTGA	504155	1.8432314	28.706198	18
CTCGT	334520	1.7944069	40.21219	42
GTCTG	318970	1.7790029	42.69394	17
CGTCT	330380	1.7721999	41.041767	16
AAGAG	463560	1.7644554	29.945618	7
CACGT	328290	1.7621268	41.260937	14
GTCAC	322685	1.7320412	40.466427	29
GATCG	310145	1.7309006	42.821503	1
ATGCC	315870	1.6954612	40.153305	47
ACTCC	325955	1.6827097	39.336662	23
ACGTC	310795	1.6682205	41.096012	15
ACACG	310460	1.6674993	41.264904	13
TGAAC	455520	1.6664938	28.52624	20
CTTGA	455540	1.665491	27.794495	35
CAGTC	304570	1.6348073	40.382698	27
TCACA	463465	1.6307423	26.903728	30
CACTT	454145	1.5969172	26.819246	33
GAATC	431945	1.5802461	27.609095	38
ACTTG	422940	1.5463026	27.693056	34
TGAAT	615270	1.5331982	19.310072	37
AACTC	435525	1.532433	27.290232	22
GAACT	415130	1.5187294	28.369053	21
ATCTC	427530	1.5033305	26.737421	40
AGTCA	374350	1.3695381	27.700436	28
ACACT	373025	1.3125212	26.478117	32
TCGTA	343435	1.2556261	27.44391	43
AATCT	517920	1.2412733	18.337858	39
GTATG	303220	1.1526607	28.377289	45
TATGC	314695	1.1505504	27.292437	46
CGTAT	309895	1.133001	27.299677	44
>>END_MODULE