FastQCFastQC Report
Wed 23 Jul 2014
LW212_CAGATC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW212_CAGATC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5533233
Filtered Sequences 0
Sequence length 51
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC 215849 3.9009562763758545 TruSeq Adapter, Index 7 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 2002605 3.5444372 4.199184 2
AAAAA 2021980 3.4212046 3.6516829 12
GGAAG 530360 2.8711295 65.28296 5
GAGCA 533940 2.7709332 62.175354 9
CACCA 575725 2.7456923 55.69168 31
TCCAG 537830 2.6998534 59.18149 25
CGGAA 510350 2.6485107 62.48847 4
CTCCA 549325 2.6434803 56.70562 24
GAAGA 709955 2.6082723 44.86345 6
CCAGA 507495 2.5247416 57.660294 33
CTGAA 692285 2.4601893 42.91797 19
TCACC 491125 2.363408 55.892822 30
TCGGA 451040 2.3618844 62.731503 3
TCTCG 462565 2.3430302 59.01729 41
AGAGC 449710 2.3338134 61.794018 8
AGCAC 461935 2.2980847 59.193295 10
ATCGG 434460 2.2750626 62.534805 2
CCAGT 448935 2.2536092 58.53834 26
GCACA 449870 2.2380621 59.101025 11
ACCAG 445395 2.2157998 57.51439 32
GTCTG 418745 2.2126014 62.327137 17
CACAC 456470 2.1769526 56.42769 12
GATCG 411870 2.1567693 62.18723 1
CGTCT 417305 2.1137745 59.54718 16
ATGCC 420125 2.1089857 57.913578 47
TCTGA 586865 2.1044168 42.94409 18
GTCAC 417950 2.0980675 58.46116 29
CTCGT 412740 2.0906515 58.421204 42
CACGT 412855 2.0724912 59.276073 14
TCATC 598255 2.0565128 40.10166 38
AAGAG 547685 2.0121157 44.28558 7
ACGTC 399775 2.0068307 59.112476 15
ACTCC 413340 1.9890884 56.31439 23
CAGTC 394560 1.980652 58.234566 27
TGAAC 552710 1.9641784 42.40553 20
ACACG 394780 1.9639949 58.700428 13
GATCA 513575 1.8251033 41.149548 36
CAGAT 506120 1.7986104 41.154278 34
GAACT 503790 1.7903303 42.19027 21
CATCT 517450 1.7787442 39.836033 39
AACTC 515220 1.7552052 40.402443 22
ATCTC 509680 1.7520347 40.145214 40
AGATC 480155 1.706338 41.09449 35
AGTCA 461095 1.6386039 41.4946 28
TCGTA 421405 1.5111002 41.325855 43
GTATG 395120 1.4779885 43.000343 45
TATGC 406510 1.4576888 41.227787 46
ATCAT 597635 1.4543524 28.432636 37
CGTAT 395455 1.4180471 41.27796 44
Produced by FastQC (version 0.10.1)