##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW212_CAGATC_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5533233	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57304852334973	34.0	31.0	34.0	31.0	34.0
2	32.79720337097679	34.0	31.0	34.0	31.0	34.0
3	32.90074880273431	34.0	31.0	34.0	31.0	34.0
4	36.32009459930568	37.0	37.0	37.0	35.0	37.0
5	36.240860632472916	37.0	37.0	37.0	35.0	37.0
6	36.2185051668708	37.0	37.0	37.0	35.0	37.0
7	36.18020658085427	37.0	37.0	37.0	35.0	37.0
8	36.17259638262116	37.0	37.0	37.0	35.0	37.0
9	37.95202750363124	39.0	38.0	39.0	35.0	39.0
10	37.89860014931596	39.0	38.0	39.0	35.0	39.0
11	37.908432556518044	39.0	38.0	39.0	35.0	39.0
12	37.90060892067983	39.0	38.0	39.0	35.0	39.0
13	37.876763367817695	39.0	38.0	39.0	35.0	39.0
14	39.38695045012563	41.0	39.0	41.0	36.0	41.0
15	39.30395792116472	41.0	39.0	41.0	36.0	41.0
16	39.31700110947795	41.0	39.0	41.0	36.0	41.0
17	39.29322152166735	40.0	39.0	41.0	36.0	41.0
18	39.24000597842166	40.0	39.0	41.0	36.0	41.0
19	39.300989132393305	40.0	39.0	41.0	36.0	41.0
20	39.282770308064016	40.0	39.0	41.0	36.0	41.0
21	39.23549396889666	40.0	39.0	41.0	36.0	41.0
22	39.13928873047638	40.0	39.0	41.0	36.0	41.0
23	39.07095255160952	40.0	39.0	41.0	36.0	41.0
24	39.02231534439269	40.0	39.0	41.0	36.0	41.0
25	39.00230624663736	40.0	38.0	41.0	36.0	41.0
26	38.89878268274624	40.0	38.0	41.0	35.0	41.0
27	38.80866737402889	40.0	38.0	41.0	35.0	41.0
28	38.57899405284397	40.0	38.0	41.0	34.0	41.0
29	38.34153197597137	40.0	38.0	41.0	34.0	41.0
30	38.26123768870749	40.0	38.0	41.0	34.0	41.0
31	38.54712895697687	40.0	38.0	41.0	34.0	41.0
32	38.5182906629813	40.0	38.0	41.0	34.0	41.0
33	38.59188163592605	40.0	38.0	41.0	35.0	41.0
34	38.709835461474334	40.0	38.0	41.0	35.0	41.0
35	38.766943846391435	40.0	38.0	41.0	35.0	41.0
36	38.775812621662595	40.0	38.0	41.0	35.0	41.0
37	38.72256454770656	40.0	38.0	41.0	35.0	41.0
38	38.68737011436171	40.0	38.0	41.0	35.0	41.0
39	38.627588789411185	40.0	38.0	41.0	35.0	41.0
40	38.57382076626811	40.0	38.0	41.0	34.0	41.0
41	38.56498253371944	40.0	38.0	41.0	34.0	41.0
42	38.50926592102664	40.0	38.0	41.0	34.0	41.0
43	38.46538325785305	40.0	38.0	41.0	34.0	41.0
44	38.39308122394267	40.0	38.0	41.0	34.0	41.0
45	38.22570439379654	40.0	38.0	41.0	33.0	41.0
46	38.09129346261037	40.0	38.0	41.0	33.0	41.0
47	38.01536190505623	40.0	37.0	41.0	33.0	41.0
48	38.00524250469843	40.0	37.0	41.0	33.0	41.0
49	37.900986457646006	40.0	37.0	41.0	33.0	41.0
50	37.81862683172749	40.0	37.0	41.0	33.0	41.0
51	36.838503999379746	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	5.0
11	3.0
12	4.0
13	6.0
14	6.0
15	13.0
16	30.0
17	55.0
18	148.0
19	368.0
20	872.0
21	1498.0
22	2517.0
23	4249.0
24	6532.0
25	9856.0
26	14533.0
27	20331.0
28	28242.0
29	38388.0
30	50027.0
31	65218.0
32	83661.0
33	108463.0
34	145401.0
35	205077.0
36	310635.0
37	529550.0
38	1143454.0
39	2762894.0
40	1192.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.93668635265757	26.029102623308063	29.853170546305236	22.18104047772913
2	18.753773788307846	32.64444132390593	29.076021197733766	19.525763690052454
3	18.753159319334646	28.282705608095664	33.72115361850838	19.242981454061304
4	18.628801642728583	27.32250747438252	29.712665271822097	24.336025611066802
5	23.468521929222934	28.13727526023213	29.759545639954073	18.634657170590867
6	23.63294659740517	28.901168629623946	27.927361815416052	19.538522957554832
7	19.537962706432207	33.143263621828325	28.72543050328804	18.59334316845143
8	18.926891385199212	33.41212994283812	28.6376879484381	19.023290723524568
9	23.218848727317283	28.341965718775985	29.03036976754819	19.408815786358534
10	18.95034964188206	32.98951987020969	28.54421637404389	19.515914113864355
11	23.452093920498196	28.448268851140014	28.523107557552702	19.57652967080909
12	18.900830671688684	28.524463003817118	28.47367895044362	24.101027374050577
13	18.929475769410036	33.17438466805934	28.392948570934927	19.50319099159569
14	18.858558820855727	28.821432244042498	28.489203328325413	23.830805606776366
15	18.809979626739015	33.28549511650783	28.54896947227778	19.35555578447537
16	18.9071560875893	28.717641205421856	28.585548448800186	23.78965425818866
17	23.45823861023022	28.577235045045096	28.629555270851597	19.334971073873085
18	19.038833174023214	28.404009012452576	33.056171681185305	19.500986132338905
19	19.0443745714516	28.376726437835227	28.536886518396	24.04201247231717
20	19.150235477416704	28.361008865170362	32.92223507186674	19.566520585546193
21	23.609199814780474	28.391199975998095	28.416846590590744	19.582753618630683
22	19.127766889784187	32.86259729613085	28.415060757895922	19.594575056189033
23	19.124052474946375	32.87955988101708	28.43185111917431	19.564536524862238
24	19.120051514187093	28.456907561998563	28.41364171723837	24.009399206575974
25	19.111033278374506	28.435003550365582	32.87166833567283	19.58229483558708
26	19.17636731063534	28.418434834568494	28.404997469869013	24.000200384927147
27	19.199743748998454	28.48350700693115	28.286290679936926	24.03045856413347
28	19.261569819405487	32.87007567144775	28.258210884800427	19.610143624346332
29	23.760973738720576	28.43701281403707	28.270723644467605	19.531289802774747
30	19.368049935984377	28.32276537356376	32.65453436301064	19.65465032744122
31	19.307274938104037	28.282619042067203	28.241858174957933	24.168247844870827
32	19.292705871032517	32.79464996765115	28.17741823752001	19.73522592379633
33	19.31465655547672	28.383074301880484	28.234877278294235	24.067391864348565
34	19.313652064154855	28.41267265148437	28.272968268128317	24.000707016232457
35	19.314986533629774	32.84270556549717	28.25222782567829	19.590080075194766
36	23.684990115860515	28.516316285956638	28.242105295502064	19.556588302680783
37	19.300602145686284	32.84483107673309	28.30693884692861	19.54762793065202
38	19.368232410547726	28.492705994787077	32.58263701810209	19.55642457656311
39	19.354410168299218	28.457342110809186	28.233742046753036	23.95450567413856
40	19.38160033393394	32.692660557922046	28.19981007416797	19.72592903397604
41	19.37395167587264	28.37641704502733	32.556844472078424	19.692786807021605
42	19.396175839368766	28.316746737395004	28.27451798482079	24.012559438415437
43	19.353993879664245	28.28449313111512	32.603467057602764	19.758045931617872
44	19.369640539925886	28.38660388525942	28.243877782345493	23.999877792469196
45	23.704641627788536	28.3949943449443	28.301683214948863	19.598680812318296
46	19.377289224999895	28.402739830522105	32.62385246143148	19.596118483046517
47	19.417586108045406	32.80476223699627	28.266780852001418	19.510870802956916
48	19.46429265356694	28.41734167045476	32.596108399448006	19.52225727653029
49	23.85045882223425	28.307984210072483	28.200498166898385	19.641058800794884
50	19.54163273924796	28.313925403549472	28.134314974682795	24.010126882519774
51	19.509020524907452	28.266896468692003	28.117358838610357	24.106724167790187
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.11772683038671
2	38.2795374783603
3	37.99614077339595
4	42.964827253795384
5	42.1031790998138
6	43.17146955496
7	38.13130587488364
8	37.95018210872378
9	42.627664513675825
10	38.46626375574642
11	43.02862359130729
12	43.00185804573926
13	38.43266676100573
14	42.68936442763209
15	38.165535411214385
16	42.696810345777955
17	42.7932096841033
18	38.53981930636212
19	43.086387043768774
20	38.7167560629629
21	43.19195343341116
22	38.72234194597322
23	38.68858899980861
24	43.12945072076307
25	38.693328113961584
26	43.17656769556248
27	43.23020231313192
28	38.87171344375182
29	43.29226354149532
30	39.0227002634256
31	43.47552278297486
32	39.02793179482885
33	43.38204841982528
34	43.31435908038731
35	38.90506660882454
36	43.2415784185413
37	38.84823007633831
38	38.924656987110836
39	43.30891584243778
40	39.10752936790998
41	39.066738482894245
42	43.4087352777842
43	39.112039811282116
44	43.36951833239509
45	43.30332244010683
46	38.973407708046416
47	38.92845691100232
48	38.98654993009723
49	43.49151762302913
50	43.55175962176773
51	43.61574469269764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	16.0
2	22.0
3	39.5
4	57.0
5	143.0
6	229.0
7	481.5
8	734.0
9	1494.0
10	2254.0
11	3703.5
12	5153.0
13	8306.5
14	11460.0
15	16658.0
16	21856.0
17	30156.5
18	38457.0
19	50752.5
20	63048.0
21	78866.0
22	94684.0
23	112942.5
24	131201.0
25	151070.0
26	189518.0
27	208097.0
28	227260.5
29	246424.0
30	263269.0
31	280114.0
32	292021.5
33	303929.0
34	316554.0
35	329179.0
36	338541.0
37	347903.0
38	353901.5
39	359900.0
40	361244.0
41	362588.0
42	360930.5
43	359273.0
44	350816.5
45	342360.0
46	325258.5
47	308157.0
48	290786.5
49	273416.0
50	262990.5
51	252565.0
52	334617.5
53	416670.0
54	288329.5
55	159989.0
56	141034.5
57	122080.0
58	107970.0
59	93860.0
60	82435.0
61	71010.0
62	61060.0
63	51110.0
64	43345.5
65	35581.0
66	30201.0
67	24821.0
68	20554.5
69	16288.0
70	13532.0
71	10776.0
72	8828.0
73	6880.0
74	5694.5
75	3552.5
76	2596.0
77	2064.5
78	1533.0
79	1175.0
80	817.0
81	603.0
82	389.0
83	277.0
84	165.0
85	126.5
86	88.0
87	61.5
88	35.0
89	24.0
90	13.0
91	10.0
92	7.0
93	6.5
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04942860710908071
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	3.4337972031902507E-4
20	1.0843570115337633E-4
21	0.006126617115165763
22	0.002259077107362007
23	3.6145233717792116E-5
24	0.0
25	0.0
26	0.07003139032822221
27	0.07733272753921623
28	0.09021850335960911
29	0.6756447812698291
30	0.102580173291094
31	0.017458147885693588
32	0.08031470932093407
33	0.025446244537325646
34	0.0021867866399264226
35	0.01686175152935002
36	0.011421893854822307
37	0.01837985134549729
38	0.0010662843946748672
39	0.010608626096171985
40	0.028916186974233687
41	0.010717061797325362
42	0.026331802763411554
43	0.00957848693521491
44	0.029982471368908557
45	0.0023675128085153836
46	0.019265409571583195
47	0.01277734011923951
48	0.009903794038675038
49	0.024434177993227467
50	0.03283794483261414
51	0.027380014541227526
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5533233.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	17.849006020244307
#Duplication Level	Relative count
1	100.0
2	9.682999112074631
3	1.5152388749870271
4	0.7362861656615044
5	0.5702325903204604
6	0.5223768724270345
7	0.4208996874963964
8	0.3413323493121461
9	0.2859811575317981
10++	1.2396360659140442
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC	215849	3.9009562763758545	TruSeq Adapter, Index 7 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2002605	3.5444372	4.199184	2
AAAAA	2021980	3.4212046	3.6516829	12
GGAAG	530360	2.8711295	65.28296	5
GAGCA	533940	2.7709332	62.175354	9
CACCA	575725	2.7456923	55.69168	31
TCCAG	537830	2.6998534	59.18149	25
CGGAA	510350	2.6485107	62.48847	4
CTCCA	549325	2.6434803	56.70562	24
GAAGA	709955	2.6082723	44.86345	6
CCAGA	507495	2.5247416	57.660294	33
CTGAA	692285	2.4601893	42.91797	19
TCACC	491125	2.363408	55.892822	30
TCGGA	451040	2.3618844	62.731503	3
TCTCG	462565	2.3430302	59.01729	41
AGAGC	449710	2.3338134	61.794018	8
AGCAC	461935	2.2980847	59.193295	10
ATCGG	434460	2.2750626	62.534805	2
CCAGT	448935	2.2536092	58.53834	26
GCACA	449870	2.2380621	59.101025	11
ACCAG	445395	2.2157998	57.51439	32
GTCTG	418745	2.2126014	62.327137	17
CACAC	456470	2.1769526	56.42769	12
GATCG	411870	2.1567693	62.18723	1
CGTCT	417305	2.1137745	59.54718	16
ATGCC	420125	2.1089857	57.913578	47
TCTGA	586865	2.1044168	42.94409	18
GTCAC	417950	2.0980675	58.46116	29
CTCGT	412740	2.0906515	58.421204	42
CACGT	412855	2.0724912	59.276073	14
TCATC	598255	2.0565128	40.10166	38
AAGAG	547685	2.0121157	44.28558	7
ACGTC	399775	2.0068307	59.112476	15
ACTCC	413340	1.9890884	56.31439	23
CAGTC	394560	1.980652	58.234566	27
TGAAC	552710	1.9641784	42.40553	20
ACACG	394780	1.9639949	58.700428	13
GATCA	513575	1.8251033	41.149548	36
CAGAT	506120	1.7986104	41.154278	34
GAACT	503790	1.7903303	42.19027	21
CATCT	517450	1.7787442	39.836033	39
AACTC	515220	1.7552052	40.402443	22
ATCTC	509680	1.7520347	40.145214	40
AGATC	480155	1.706338	41.09449	35
AGTCA	461095	1.6386039	41.4946	28
TCGTA	421405	1.5111002	41.325855	43
GTATG	395120	1.4779885	43.000343	45
TATGC	406510	1.4576888	41.227787	46
ATCAT	597635	1.4543524	28.432636	37
CGTAT	395455	1.4180471	41.27796	44
>>END_MODULE