FastQCFastQC Report
Wed 23 Jul 2014
LW211_GCCAAT_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW211_GCCAAT_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7109579
Filtered Sequences 0
Sequence length 51
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC 196281 2.760796384708574 TruSeq Adapter, Index 6 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 2544415 3.4619336 4.106589 1
AAAAA 2585570 3.4168656 3.7421825 3
CGCCA 488545 2.7044356 57.781544 33
GGAAG 593565 2.4886096 46.303898 5
GAGCA 589340 2.384346 44.41319 9
GAAGA 827665 2.3615878 32.08157 6
TCCAG 600825 2.3593812 42.019943 25
CTCCA 611030 2.315408 40.53638 24
CGGAA 568330 2.2993438 44.49297 4
CTGAA 801455 2.2196033 30.621231 19
ACGCC 396305 2.1938236 57.508896 32
GCCAA 554950 2.1665668 40.958107 34
CACGC 384355 2.127672 57.756897 31
TCTCG 501840 1.982199 41.86822 41
TCGGA 486970 1.9816978 44.460773 3
AGAGC 484555 1.9604077 44.032307 8
AGCAC 499855 1.9514719 42.467052 10
CCAGT 488925 1.9199609 41.540936 26
ATCGG 467280 1.9015704 44.258236 2
GCACA 486645 1.899899 42.302074 11
CACAC 492180 1.8542024 40.679714 12
TCTGA 663835 1.8492185 30.490904 18
GTCTG 445410 1.8231695 43.89014 17
GATCG 439870 1.7900268 43.99035 1
ATGCC 455110 1.7871724 41.3844 47
CGTCT 444225 1.7546276 42.223507 16
AAGAG 612260 1.7469697 31.443863 7
GTCAC 444035 1.7436821 41.562286 29
CTCGT 438745 1.7329823 41.468243 42
CACGT 435875 1.7116387 42.04697 14
TGAAC 615925 1.7057841 30.072319 20
TCACG 430880 1.6920238 41.30307 30
ACGTC 424150 1.6655958 42.001053 15
ACTCC 436960 1.6557955 40.02457 23
CCAAT 617350 1.6498431 28.299595 35
CAGTC 417990 1.6414059 41.40169 27
ACACG 415835 1.6234516 41.874054 13
GAACT 554340 1.5352265 29.85965 21
AACTC 571875 1.5283129 28.734411 22
ATCTC 565430 1.5199239 28.567636 40
AGTCA 503685 1.3949391 29.47961 28
AATAT 961650 1.2857375 14.683524 37
TCGTA 449060 1.2509284 29.29176 43
TATGC 436425 1.2157316 29.272171 46
CAATA 643480 1.212809 20.034342 36
GTATG 415930 1.2006971 30.308231 45
CGTAT 418420 1.1655759 29.238111 44
ATATC 601385 1.1400968 20.122032 38
TATCT 515560 0.9831056 20.139376 39
Produced by FastQC (version 0.10.1)