##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW211_GCCAAT_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7109579	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55881789906266	34.0	31.0	34.0	31.0	34.0
2	32.786352609627095	34.0	31.0	34.0	31.0	34.0
3	32.888401690170404	34.0	31.0	34.0	31.0	34.0
4	36.311877820050945	37.0	37.0	37.0	35.0	37.0
5	36.23333350680821	37.0	37.0	37.0	35.0	37.0
6	36.2101620925796	37.0	37.0	37.0	35.0	37.0
7	36.17653323776274	37.0	37.0	37.0	35.0	37.0
8	36.1642665479911	37.0	37.0	37.0	35.0	37.0
9	37.94456324347757	39.0	38.0	39.0	35.0	39.0
10	37.89714383931876	39.0	38.0	39.0	35.0	39.0
11	37.899292208441594	39.0	38.0	39.0	35.0	39.0
12	37.88524847955132	39.0	38.0	39.0	35.0	39.0
13	37.860348130318265	39.0	38.0	39.0	35.0	39.0
14	39.363364412998294	41.0	39.0	41.0	36.0	41.0
15	39.304716495871276	41.0	39.0	41.0	36.0	41.0
16	39.297412406557406	41.0	39.0	41.0	36.0	41.0
17	39.2825983085637	40.0	39.0	41.0	36.0	41.0
18	39.22042796064296	40.0	39.0	41.0	36.0	41.0
19	39.27903874476956	40.0	39.0	41.0	36.0	41.0
20	39.263466092718005	40.0	39.0	41.0	36.0	41.0
21	39.208027929642526	40.0	39.0	41.0	36.0	41.0
22	39.14887548193782	40.0	39.0	41.0	36.0	41.0
23	39.08850186487835	40.0	39.0	41.0	36.0	41.0
24	38.998024636901846	40.0	38.0	41.0	36.0	41.0
25	38.97942803645617	40.0	38.0	41.0	36.0	41.0
26	38.84493498138216	40.0	38.0	41.0	35.0	41.0
27	38.753127013568594	40.0	38.0	41.0	35.0	41.0
28	38.611275857543745	40.0	38.0	41.0	35.0	41.0
29	38.3177795759777	40.0	38.0	41.0	34.0	41.0
30	38.29303507282217	40.0	38.0	41.0	34.0	41.0
31	38.5398903366852	40.0	38.0	41.0	34.0	41.0
32	38.502989698827456	40.0	38.0	41.0	34.0	41.0
33	38.551449670929884	40.0	38.0	41.0	34.0	41.0
34	38.67470028816052	40.0	38.0	41.0	35.0	41.0
35	38.754720216204085	40.0	38.0	41.0	35.0	41.0
36	38.748819022898545	40.0	38.0	41.0	35.0	41.0
37	38.679573853810474	40.0	38.0	41.0	35.0	41.0
38	38.61887658889507	40.0	38.0	41.0	35.0	41.0
39	38.603658669521785	40.0	38.0	41.0	35.0	41.0
40	38.526913056314584	40.0	38.0	41.0	34.0	41.0
41	38.52035317421749	40.0	38.0	41.0	34.0	41.0
42	38.4518129695162	40.0	38.0	41.0	34.0	41.0
43	38.40986308753303	40.0	38.0	41.0	34.0	41.0
44	38.32973190114351	40.0	38.0	41.0	34.0	41.0
45	38.195796966318255	40.0	38.0	41.0	33.0	41.0
46	38.092843472166216	40.0	38.0	41.0	33.0	41.0
47	38.00645073358071	40.0	37.0	41.0	33.0	41.0
48	37.971073392672054	40.0	37.0	41.0	33.0	41.0
49	37.87491256514626	40.0	37.0	41.0	33.0	41.0
50	37.78069995424483	40.0	37.0	41.0	33.0	41.0
51	36.774387625483875	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	5.0
11	8.0
12	2.0
13	8.0
14	6.0
15	14.0
16	36.0
17	109.0
18	238.0
19	523.0
20	1181.0
21	1943.0
22	3367.0
23	5505.0
24	8767.0
25	13131.0
26	18843.0
27	26684.0
28	36736.0
29	49563.0
30	65540.0
31	84373.0
32	108464.0
33	140675.0
34	188618.0
35	266104.0
36	404080.0
37	691466.0
38	1488672.0
39	3503527.0
40	1384.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.863453213375895	26.886021997450953	31.07468182303674	21.17584296613641
2	18.870217209767272	31.736604938210828	29.41909781155818	19.97408004046372
3	19.159798350929076	28.575067525095367	32.57981942390681	19.685314700068737
4	19.18598836865024	27.77573749444236	29.67056136516663	23.367712771740774
5	22.479263540077408	28.585602607411776	29.911785212598385	19.02334863991243
6	22.618371636351466	29.149095888800165	28.32903326624544	19.90349920860293
7	19.83477502676319	32.092617579746985	29.069484986382456	19.00312240710737
8	19.41318888221089	32.41393055763218	28.798189034821892	19.374691525335045
9	22.283752666648756	28.812732793320112	29.175046792503466	19.728467747527667
10	19.29866733318527	32.09683723888573	28.808569396303213	19.795926031625783
11	22.463566408081267	28.877195119429715	28.79840001777883	19.860838454710187
12	19.225189002049206	28.867869672733082	28.8317493905054	23.075191934712308
13	19.16024844790388	32.19934682489639	28.868390097360198	19.772014629839543
14	19.17030530218456	29.08553375664016	28.925664937403468	22.818496003771813
15	19.094773403600975	32.268155399918896	28.970168275786794	19.666902920693335
16	19.20563791470634	29.058781117700498	28.978115300498104	22.757465667095055
17	22.3699884339143	28.97658215767769	28.99406561204257	19.65936379636544
18	19.293449021383687	28.827037437800467	32.07917093262485	19.800342608191006
19	19.332103719125847	28.80238361178286	28.908381926443415	22.95713074264788
20	19.424193186312763	28.702627950036934	31.999563405504578	19.87361545814572
21	22.569435066776496	28.689151125995345	28.799137104506233	19.942276702721927
22	19.41779705801226	31.87676166140908	28.829842584709198	19.875598695869463
23	19.467923337773822	31.881418542903468	28.79044702659525	19.860211092727457
24	19.45375660833878	28.741888759079654	28.75487124552259	23.049483387058977
25	19.459703591450353	28.75991672643345	31.848819740240597	19.9315599418756
26	19.510111299953902	28.811592366997786	28.708068982747275	22.970227350301034
27	19.490292922579016	28.83379658055702	28.65912521019262	23.016785286671347
28	19.55489364014511	31.92691950815474	28.61538405226535	19.902802799434795
29	22.738817475331615	28.77578471906917	28.598389307444123	19.887008498155094
30	19.6572559780975	28.611360831607975	31.694423774548973	20.036959415745557
31	19.63462874630316	28.683926616258056	28.59541213045167	23.086032506987117
32	19.653807026140502	31.77362982349133	28.53754604652027	20.035017103847895
33	19.636187697501956	28.723363395438923	28.56235810564699	23.07809080141213
34	22.68947148253996	28.7188122148683	28.606327873639447	19.98538842895229
35	19.632279426670348	28.773116355611588	28.59908382161433	22.99552039610373
36	19.583847306583234	28.77525748600368	28.60556718662543	23.03532802078766
37	19.563011775748425	31.90892160727304	28.632459514446275	19.895607102532264
38	19.620324650344365	31.90619024276689	28.61128673696233	19.862198369926414
39	19.691098454929598	28.755074472486264	31.618554686703238	19.9352723858809
40	19.687967892695013	31.792504037190266	28.580563577410643	19.938964492704077
41	19.720192052864615	28.675981969582814	31.580759039825708	20.023066937726863
42	19.68788065607979	28.649395297612735	28.56991916197909	23.092804884328384
43	19.67508933861572	28.629713593142576	31.609200738003647	20.08599633023806
44	19.710478642070008	28.665974254888198	28.570244715318584	23.053302387723207
45	22.765454458246317	28.68146365224397	28.593692791578952	19.95938909793076
46	19.662744706338326	28.747760529468163	31.65941337302422	19.930081391169292
47	19.72788292663713	31.786760035758977	28.622667560406573	19.862689477197325
48	19.776494065040215	28.688808695135982	31.588486197712328	19.94621104211147
49	22.854015558711662	28.655716901510498	28.514578466399783	19.97568907337806
50	19.835409127491094	28.638797746548626	28.467129663501854	23.058663462458423
51	19.798459661612917	28.648265202368318	28.434087791775042	23.119187344243723
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.039296179512306
2	38.844297250230994
3	38.84511305099782
4	42.55370114039101
5	41.50261217998984
6	42.52187084495439
7	38.83789743387055
8	38.787880407545934
9	42.01222041417642
10	39.094593364811054
11	42.324404862791454
12	42.30038093676151
13	38.93226307774342
14	41.98880130595637
15	38.76167632429431
16	41.9631035818014
17	42.029352230279734
18	39.093791629574696
19	42.289234461773724
20	39.297808644458485
21	42.511711769498426
22	39.29339575388172
23	39.32813443050128
24	42.503239995397756
25	39.391263533325954
26	42.48033865025494
27	42.50707820925036
28	39.45769643957991
29	42.625825973486705
30	39.69421539384305
31	42.720661253290274
32	39.6888241299884
33	42.71427849891409
34	42.674859911492256
35	42.62779982277408
36	42.61917532737089
37	39.45861887828069
38	39.482523020270776
39	39.6263708408105
40	39.62693238539909
41	39.74325899059148
42	42.78068554040818
43	39.76108566885378
44	42.76378102979322
45	42.72484355617708
46	39.59282609750762
47	39.59057240383446
48	39.72270510715169
49	42.82970463208972
50	42.894072589949516
51	42.917647005856644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	20.5
2	19.0
3	50.0
4	81.0
5	183.0
6	285.0
7	643.0
8	1001.0
9	1858.5
10	2716.0
11	4764.0
12	6812.0
13	10729.0
14	14646.0
15	21317.5
16	27989.0
17	39140.0
18	50291.0
19	66068.0
20	81845.0
21	102000.5
22	122156.0
23	146221.5
24	170287.0
25	195230.5
26	245027.0
27	269880.0
28	294342.5
29	318805.0
30	340477.0
31	362149.0
32	378542.0
33	394935.0
34	411062.0
35	427189.0
36	439249.5
37	451310.0
38	458756.0
39	466202.0
40	468542.5
41	470883.0
42	468088.5
43	465294.0
44	454413.5
45	443533.0
46	421279.5
47	399026.0
48	378783.5
49	358541.0
50	341621.0
51	324701.0
52	391331.0
53	457961.0
54	334130.5
55	210300.0
56	186082.5
57	161865.0
58	144206.0
59	126547.0
60	110690.0
61	94833.0
62	81372.0
63	67911.0
64	57438.5
65	46966.0
66	39817.5
67	32669.0
68	27269.0
69	21869.0
70	18177.0
71	14485.0
72	11912.5
73	9340.0
74	7651.0
75	4829.0
76	3696.0
77	2927.5
78	2159.0
79	1682.5
80	1206.0
81	876.0
82	546.0
83	412.5
84	279.0
85	203.0
86	127.0
87	88.5
88	50.0
89	34.0
90	18.0
91	14.5
92	11.0
93	7.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04771027932877601
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	4.5009697479977365E-4
20	9.845871323745048E-5
21	0.0069061754570840265
22	0.0023630091176988118
23	2.8131060924985853E-5
24	1.4065530462492927E-5
25	0.0
26	0.0689773613880653
27	0.07568661941867444
28	0.08844405554815551
29	0.6824173414487693
30	0.10118742614717413
31	0.016006573666316953
32	0.07907641226013523
33	0.024445891943812706
34	0.0020395019170614745
35	0.016006573666316953
36	0.010492885725019723
37	0.01745532330395372
38	0.0011815045588494059
39	0.010014657689294965
40	0.02690735977474897
41	0.009888067915132527
42	0.025106971875549874
43	0.009437970940332755
44	0.028904665100422965
45	0.0022223538130738824
46	0.018482107027715706
47	0.012293273624218816
48	0.009634888366807655
49	0.02340504268958823
50	0.03177403331477152
51	0.02700581848798642
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7109579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	22.65741437396067
#Duplication Level	Relative count
1	100.0
2	16.03135262407832
3	2.720118966478716
4	0.8104591362537952
5	0.4839209368610199
6	0.4194807608897701
7	0.3884999070574385
8	0.36433484106821984
9	0.3494640312287007
10++	2.3557841254104965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	196281	2.760796384708574	TruSeq Adapter, Index 6 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2544415	3.4619336	4.106589	1
AAAAA	2585570	3.4168656	3.7421825	3
CGCCA	488545	2.7044356	57.781544	33
GGAAG	593565	2.4886096	46.303898	5
GAGCA	589340	2.384346	44.41319	9
GAAGA	827665	2.3615878	32.08157	6
TCCAG	600825	2.3593812	42.019943	25
CTCCA	611030	2.315408	40.53638	24
CGGAA	568330	2.2993438	44.49297	4
CTGAA	801455	2.2196033	30.621231	19
ACGCC	396305	2.1938236	57.508896	32
GCCAA	554950	2.1665668	40.958107	34
CACGC	384355	2.127672	57.756897	31
TCTCG	501840	1.982199	41.86822	41
TCGGA	486970	1.9816978	44.460773	3
AGAGC	484555	1.9604077	44.032307	8
AGCAC	499855	1.9514719	42.467052	10
CCAGT	488925	1.9199609	41.540936	26
ATCGG	467280	1.9015704	44.258236	2
GCACA	486645	1.899899	42.302074	11
CACAC	492180	1.8542024	40.679714	12
TCTGA	663835	1.8492185	30.490904	18
GTCTG	445410	1.8231695	43.89014	17
GATCG	439870	1.7900268	43.99035	1
ATGCC	455110	1.7871724	41.3844	47
CGTCT	444225	1.7546276	42.223507	16
AAGAG	612260	1.7469697	31.443863	7
GTCAC	444035	1.7436821	41.562286	29
CTCGT	438745	1.7329823	41.468243	42
CACGT	435875	1.7116387	42.04697	14
TGAAC	615925	1.7057841	30.072319	20
TCACG	430880	1.6920238	41.30307	30
ACGTC	424150	1.6655958	42.001053	15
ACTCC	436960	1.6557955	40.02457	23
CCAAT	617350	1.6498431	28.299595	35
CAGTC	417990	1.6414059	41.40169	27
ACACG	415835	1.6234516	41.874054	13
GAACT	554340	1.5352265	29.85965	21
AACTC	571875	1.5283129	28.734411	22
ATCTC	565430	1.5199239	28.567636	40
AGTCA	503685	1.3949391	29.47961	28
AATAT	961650	1.2857375	14.683524	37
TCGTA	449060	1.2509284	29.29176	43
TATGC	436425	1.2157316	29.272171	46
CAATA	643480	1.212809	20.034342	36
GTATG	415930	1.2006971	30.308231	45
CGTAT	418420	1.1655759	29.238111	44
ATATC	601385	1.1400968	20.122032	38
TATCT	515560	0.9831056	20.139376	39
>>END_MODULE