##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW210_ACAGTG_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4222456	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.579687508881086	34.0	31.0	34.0	31.0	34.0
2	32.791361946696426	34.0	31.0	34.0	31.0	34.0
3	32.90168589086541	34.0	31.0	34.0	31.0	34.0
4	36.32328981047997	37.0	37.0	37.0	35.0	37.0
5	36.25279008235965	37.0	37.0	37.0	35.0	37.0
6	36.226767312673005	37.0	37.0	37.0	35.0	37.0
7	36.138988778095026	37.0	37.0	37.0	35.0	37.0
8	36.14829757847092	37.0	37.0	37.0	35.0	37.0
9	37.9511554412882	39.0	38.0	39.0	35.0	39.0
10	37.85025089663456	39.0	38.0	39.0	35.0	39.0
11	37.89075931164233	39.0	38.0	39.0	35.0	39.0
12	37.867135856477844	39.0	38.0	39.0	35.0	39.0
13	37.8569415524993	39.0	38.0	39.0	35.0	39.0
14	39.36910603686575	41.0	39.0	41.0	36.0	41.0
15	39.29539774955618	41.0	39.0	41.0	36.0	41.0
16	39.32109677401019	41.0	39.0	41.0	36.0	41.0
17	39.29990531576883	41.0	39.0	41.0	36.0	41.0
18	39.19437952698619	40.0	39.0	41.0	36.0	41.0
19	39.29795834462218	40.0	39.0	41.0	36.0	41.0
20	39.29752471073707	40.0	39.0	41.0	36.0	41.0
21	39.24703253272503	40.0	39.0	41.0	36.0	41.0
22	39.05484912098551	40.0	39.0	41.0	36.0	41.0
23	39.03522286555502	40.0	39.0	41.0	36.0	41.0
24	39.03887192667017	40.0	39.0	41.0	36.0	41.0
25	39.0162907559013	40.0	38.0	41.0	36.0	41.0
26	38.94286382143473	40.0	38.0	41.0	36.0	41.0
27	38.85892641628474	40.0	38.0	41.0	35.0	41.0
28	38.54216716527064	40.0	38.0	41.0	34.0	41.0
29	38.34674156462495	40.0	38.0	41.0	34.0	41.0
30	38.25533552037014	40.0	38.0	41.0	34.0	41.0
31	38.55866254142139	40.0	38.0	41.0	35.0	41.0
32	38.522668560667064	40.0	38.0	41.0	34.0	41.0
33	38.64044930249125	40.0	38.0	41.0	35.0	41.0
34	38.73987816569314	40.0	38.0	41.0	35.0	41.0
35	38.83784555718283	40.0	38.0	41.0	35.0	41.0
36	38.79418281682509	40.0	38.0	41.0	35.0	41.0
37	38.745359572722606	40.0	38.0	41.0	35.0	41.0
38	38.45393273488226	40.0	38.0	41.0	34.0	41.0
39	38.54054867593647	40.0	38.0	41.0	34.0	41.0
40	38.40303107954233	40.0	38.0	41.0	34.0	41.0
41	38.49939750704329	40.0	38.0	41.0	34.0	41.0
42	38.52191852324808	40.0	38.0	41.0	34.0	41.0
43	38.50999086787405	40.0	38.0	41.0	34.0	41.0
44	38.472695274977404	40.0	38.0	41.0	34.0	41.0
45	38.32833095241253	40.0	38.0	41.0	34.0	41.0
46	38.13386782479202	40.0	38.0	41.0	33.0	41.0
47	38.06349835261753	40.0	38.0	41.0	33.0	41.0
48	38.10199798411162	40.0	38.0	41.0	33.0	41.0
49	38.017828486549064	40.0	37.0	41.0	33.0	41.0
50	37.93773718423591	40.0	37.0	41.0	33.0	41.0
51	36.93120828257299	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	1.0
10	5.0
11	4.0
12	5.0
13	4.0
14	9.0
15	10.0
16	23.0
17	43.0
18	138.0
19	274.0
20	650.0
21	1139.0
22	1845.0
23	3030.0
24	4781.0
25	7367.0
26	10907.0
27	15618.0
28	21559.0
29	29090.0
30	38686.0
31	50374.0
32	64827.0
33	83421.0
34	113179.0
35	157252.0
36	237153.0
37	397448.0
38	854696.0
39	2127938.0
40	976.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.195994223303213	25.509021053666654	29.41573142861882	19.879253294411317
2	17.733186562512433	35.661591263473206	27.724575460348195	18.88064671366617
3	18.097903210832747	27.0211933528733	36.38846680699574	18.492436629298208
4	17.82974174272035	26.47269740643834	28.26961370349389	27.42794714734742
5	26.615600020462026	27.155546440270783	28.484843891801358	17.744009647465838
6	26.7565606367479	27.661578948365594	27.063206816127867	18.518653598758636
7	18.45343089424733	36.08463889262553	27.76137868576961	17.70055152735754
8	18.031709507452533	36.42413325325356	27.46003747581976	18.08411976347415
9	26.385236459539186	27.38825460821853	27.815280964443446	18.411227967798833
10	17.934846449554477	36.1133899323048	27.40322220053921	18.54854141760151
11	26.55613226046642	27.40570890495958	27.416295160920562	18.621863673653436
12	17.88494658085247	27.443483129249895	27.46998429350122	27.20158599639641
13	17.81096120362178	36.21977351569797	27.463021521124197	18.50624375955605
14	17.754051196744264	27.742408683477105	27.541838209800172	26.96170190997846
15	17.763950648627244	36.272681112603664	27.596616755745945	18.36675148302315
16	17.854987713311875	27.60961866742957	27.626504574588818	26.90888904466974
17	26.44977235997249	27.518321090853288	27.645948234866154	18.38595831430807
18	17.94116978365198	27.36180080976569	36.193603912036025	18.503425494546303
19	17.968195670564253	27.337058821857674	27.6346589794915	27.06008652808658
20	18.08861296706274	27.30683498592761	36.04112018324898	18.563431863760677
21	26.62710583843929	27.370724671319863	27.440854153886963	18.561315336353886
22	18.085249995381727	35.91545292938211	27.432112577853417	18.56718449738274
23	18.09554777755963	35.912039755090234	27.415888347365858	18.576524119984285
24	18.097121675157776	27.357774716894625	27.459587500734166	27.08551610721343
25	18.058518549394	27.39753830472123	35.91165899656503	18.632284149319734
26	18.126351927994186	27.44731109861339	27.37398378030055	27.05235319309188
27	18.16456143228306	27.483369936143774	27.311274313454366	27.040794318118795
28	18.312004225050302	35.823917994580256	27.261222078732334	18.60285570163711
29	26.750726528619072	27.428589413808	27.280064741401596	18.54061931617133
30	18.30482187718773	27.255733525456428	35.6718820121433	18.767562585212545
31	18.231407664067508	27.35096060806968	27.312256306799572	27.105375421063236
32	18.25339432367021	35.733459049463754	27.21398691499869	18.799159711867354
33	18.22222295921256	27.461776061958986	27.204774370803054	27.111226608025397
34	18.264450106919412	35.7944021074575	27.185713889860573	18.755433895762515
35	18.266819272702122	27.56082528535465	27.252210213550544	26.920145228392684
36	18.396846597294775	35.80200185742279	27.223110299695946	18.578041245586487
37	26.65362442220678	27.4288870791768	27.394872071831355	18.522616426785067
38	18.482637624141308	27.392510336523774	35.615334006251416	18.50951803308351
39	26.78828882067976	27.407263364158503	27.23106653911207	18.573381276049666
40	18.41474557435908	35.731594386785154	27.2576649634599	18.59599507539586
41	18.35705483139542	27.311552236522257	35.60763802946438	18.72375490261794
42	18.32484000386606	27.23231798722305	27.360855005694855	27.081987003216028
43	18.31093167042791	27.193884995007117	35.640103003120785	18.855080331444185
44	18.440646101094075	27.28780485724784	27.23073573680641	27.040813304851678
45	26.729937939743483	27.299976221760975	27.395918100545003	18.574167737950543
46	18.356892074797365	27.449364536960232	35.65090488401917	18.542838504223234
47	18.39784455958549	35.73537823834197	27.346025166543303	18.520752035529238
48	18.51785006687543	27.293393768730628	35.581091461001336	18.607664703392608
49	26.812538316418046	27.279820951583204	27.179025675671838	18.728615056326912
50	18.51633021811134	27.22481646376147	27.129035765787172	27.12981755234002
51	18.556894453579336	27.17504730179028	27.13963166314445	27.128426581485936
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	45.07524751771454
2	36.6138332761786
3	36.59033984013096
4	45.25768889006777
5	44.359609667927856
6	45.27521423550654
7	36.153982421604866
8	36.11582927092669
9	44.79646442733802
10	36.48338786715598
11	45.177995934119856
12	45.08653257724888
13	36.31720496317783
14	44.71575310672272
15	36.130702131650395
16	44.76387675798161
17	44.83573067428056
18	36.444595278198285
19	45.02828219865083
20	36.652044830823414
21	45.18842117479318
22	36.65243449276447
23	36.672071897543915
24	45.18263778237121
25	36.69080269871374
26	45.17870512108606
27	45.20535575040186
28	36.91485992668741
29	45.291345844790406
30	37.07238446240027
31	45.33678308513075
32	37.05255403553756
33	45.33344956723796
34	37.01988400268193
35	45.1869645010948
36	36.974887842881266
37	45.176240848991846
38	36.992155657224814
39	45.361670096729426
40	37.01074064975494
41	37.080809734013364
42	45.40682700708209
43	37.1660120018721
44	45.481459405945756
45	45.304105677694025
46	36.8997305790206
47	36.91859659511473
48	37.12551477026804
49	45.54115337274496
50	45.64614777045136
51	45.68532103506527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	16.0
2	16.0
3	35.5
4	55.0
5	113.0
6	171.0
7	373.0
8	575.0
9	1114.5
10	1654.0
11	2800.0
12	3946.0
13	6412.0
14	8878.0
15	12896.0
16	16914.0
17	23452.0
18	29990.0
19	39499.5
20	49009.0
21	61144.5
22	73280.0
23	87561.0
24	101842.0
25	116530.0
26	145258.5
27	159299.0
28	172727.5
29	186156.0
30	198386.0
31	210616.0
32	219845.5
33	229075.0
34	237262.5
35	245450.0
36	251821.0
37	258192.0
38	261885.5
39	265579.0
40	265370.0
41	265161.0
42	262596.5
43	260032.0
44	252825.5
45	245619.0
46	233045.5
47	220472.0
48	207297.0
49	194122.0
50	190051.0
51	185980.0
52	325719.5
53	465459.0
54	289617.5
55	113776.0
56	97535.0
57	81294.0
58	72033.5
59	62773.0
60	54785.5
61	46798.0
62	40680.0
63	34562.0
64	29311.0
65	24060.0
66	20477.0
67	16894.0
68	14329.5
69	11765.0
70	9889.0
71	8013.0
72	6719.5
73	5426.0
74	4413.0
75	2793.0
76	2186.0
77	1751.0
78	1316.0
79	1027.0
80	738.0
81	544.5
82	351.0
83	274.0
84	197.0
85	139.5
86	82.0
87	55.0
88	28.0
89	21.0
90	14.0
91	7.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04857362634447819
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.8419479089894604E-4
20	9.473159696631534E-5
21	0.00629965119825997
22	0.0023209241256747257
23	7.104869772473651E-5
24	0.0
25	0.0
26	0.0714986728103265
27	0.07791673850479437
28	0.0901844803119322
29	0.67590994435466
30	0.10247590501831162
31	0.016649078166829923
32	0.08116129570089066
33	0.025648579878629882
34	0.002770899211264724
35	0.01638856627517255
36	0.011296742938233104
37	0.01870949040084728
38	0.0011841449620789417
39	0.011202011341266789
40	0.028490527787619336
41	0.011296742938233104
42	0.026145920762703032
43	0.010609938860227318
44	0.029816770145147754
45	0.002557753118090514
46	0.019183148385678857
47	0.013759764459357303
48	0.010112597976154162
49	0.02434602042034304
50	0.03209032847233932
51	0.02740111442250671
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4222456.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	20.692230737543525
#Duplication Level	Relative count
1	100.0
2	9.288918823274239
3	1.4874599025103026
4	0.836196760236989
5	0.6130206965832943
6	0.49601022842726517
7	0.3504606216965947
8	0.23116702245459422
9	0.17237639699083324
10++	0.6809438463909405
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC	321247	7.608060332659476	TruSeq Adapter, Index 5 (100% over 51bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	880270	5.3822885	105.16645	12
GGAAG	566590	3.9750345	122.42379	5
GAGCA	573310	3.8419755	116.19314	9
TTTTT	1542815	3.723958	4.367069	1
CGGAA	555440	3.7222216	116.69973	4
TCCAG	565395	3.6903594	110.64424	25
CTCCA	580870	3.6215014	105.59225	24
TCGGA	513760	3.5106208	118.796005	3
CAGTG	510285	3.486876	114.53764	35
TCTCG	521590	3.4713998	112.65553	41
AGAGC	513485	3.4410646	115.86747	8
ATCGG	499485	3.4130774	118.5618	2
GTCTG	482975	3.36517	119.31884	17
AAAAA	1535575	3.3625498	3.6263413	13
AGCAC	520265	3.330295	110.611664	10
GAAGA	699710	3.3299065	83.47181	6
GCACA	513840	3.2891676	110.43133	11
CCAGT	503595	3.2869883	110.06875	26
GATCG	479640	3.2774727	118.07816	1
CACAG	507650	3.2495441	107.82862	33
CTCGT	486260	3.2362635	112.20351	42
CGTCT	484540	3.2248166	113.691765	16
CTGAA	686245	3.1808617	80.09047	19
ATGCC	487135	3.1795528	110.21083	47
CACGT	485170	3.1667273	112.068474	14
GTCAC	482505	3.1493328	110.241554	29
ACGTC	472355	3.0830832	111.67033	15
CAGTC	468335	3.0568445	109.69074	27
ACACG	474925	3.0400665	109.966965	13
ACTCC	484890	3.0231032	105.6613	23
TCTGA	611035	2.8879542	81.51807	18
AAGAG	584220	2.7802913	82.87184	7
TGAAC	580265	2.6896265	79.54179	20
AGTGA	551625	2.6768038	81.62916	36
GTGAT	533200	2.638283	83.14979	37
TGATC	555305	2.624556	79.28057	38
ACAGT	553950	2.5676517	77.78335	34
TCACA	578965	2.5633678	74.824524	30
GAACT	548605	2.5428774	79.228264	21
ATCTC	552140	2.4926798	76.27419	40
AACTC	560840	2.4831192	75.58869	22
GATCT	525105	2.4818208	79.57039	39
ACACA	567490	2.4640992	73.39687	32
AGTCA	515850	2.391052	78.18044	28
GTATG	471730	2.3341284	83.49014	45
TCGTA	493210	2.3310742	79.874954	43
TATGC	483440	2.284898	79.72691	46
CGTAT	473485	2.2378476	79.75286	44
>>END_MODULE