FastQCFastQC Report
Wed 23 Jul 2014
LW209_TGACCA_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW209_TGACCA_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6596382
Filtered Sequences 0
Sequence length 51
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC 359269 5.446455344763235 TruSeq Adapter, Index 4 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 2913350 3.817884 4.4828944 1
AAAAA 2862065 3.6011858 3.7689846 3
GGAAG 718565 3.5128407 96.87403 5
GAGCA 726320 3.374974 91.71834 9
CGGAA 721895 3.3544126 91.986694 4
TCCAG 718665 3.2000139 85.77631 25
CTCCA 735245 3.1117704 81.305405 24
TCGGA 649155 3.041051 92.46857 3
TCTCG 658105 2.9542913 85.68689 41
AGAGC 632935 2.941044 91.38452 8
GAAGA 941705 2.9284093 62.201115 6
ATCGG 620640 2.9074686 92.14413 2
CACTG 642740 2.8619409 85.40792 31
GCACA 646505 2.8553834 86.52255 11
CTGAA 946660 2.82094 58.800365 19
GTCTG 595490 2.8124354 91.61408 17
AGCAC 636370 2.810621 86.544334 10
CCAGT 621575 2.7676992 85.07643 26
CACAC 651410 2.7346208 81.75737 12
GATCG 582860 2.7304833 91.6179 1
GTCAC 603900 2.6889975 85.48583 29
CTGAC 601415 2.6779325 84.72142 33
CTCGT 594390 2.6682694 84.87485 42
CGTCT 593895 2.6660469 86.78175 16
CACGT 596755 2.657183 86.42223 14
ATGCC 590800 2.630667 84.26386 47
GACCA 590940 2.6099725 82.88874 35
TGACC 576980 2.5691304 83.783905 34
ACGTC 574530 2.558221 86.04721 15
ACACG 577480 2.5505247 85.77392 13
CAGTC 571190 2.543349 84.826996 27
ACTCC 592005 2.505537 81.17766 23
TCTGA 817630 2.4563456 58.91027 18
AAGAG 752425 2.3398077 61.660877 7
TGAAC 767220 2.2862294 58.20269 20
ACCAA 788120 2.2141626 53.072483 36
ACTGA 729475 2.1737533 57.43794 32
CCAAT 764120 2.1642702 53.468758 37
GAACT 711660 2.1206663 57.968903 21
TCACT 736150 2.102079 54.987225 30
AACTC 728185 2.0624893 54.976334 22
ATCTC 709575 2.0261939 54.505707 40
CAATC 707790 2.004723 53.368027 38
AGTCA 661960 1.9725661 57.18139 28
GTATG 579765 1.83246 59.696526 45
TATGC 591585 1.7772552 56.664455 46
TCGTA 589065 1.7696846 56.837173 43
CGTAT 566735 1.7026001 56.75026 44
AATCT 839900 1.6050359 36.556973 39
Produced by FastQC (version 0.10.1)