##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW209_TGACCA_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6596382	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.582737021597595	34.0	31.0	34.0	31.0	34.0
2	32.80238303360842	34.0	31.0	34.0	31.0	34.0
3	32.90844556910137	34.0	31.0	34.0	31.0	34.0
4	36.3254602295622	37.0	37.0	37.0	35.0	37.0
5	36.254804982488885	37.0	37.0	37.0	35.0	37.0
6	36.23385455845341	37.0	37.0	37.0	35.0	37.0
7	36.170124774459694	37.0	37.0	37.0	35.0	37.0
8	36.16912498396849	37.0	37.0	37.0	35.0	37.0
9	37.96836417296633	39.0	38.0	39.0	35.0	39.0
10	37.8885211620552	39.0	38.0	39.0	35.0	39.0
11	37.91491396344239	39.0	38.0	39.0	35.0	39.0
12	37.89441211864322	39.0	38.0	39.0	35.0	39.0
13	37.88117668139898	39.0	38.0	39.0	35.0	39.0
14	39.39535642417313	41.0	39.0	41.0	36.0	41.0
15	39.33545161574936	41.0	39.0	41.0	36.0	41.0
16	39.30128561384104	41.0	39.0	41.0	36.0	41.0
17	39.31005708887084	41.0	39.0	41.0	36.0	41.0
18	39.22554879326273	40.0	39.0	41.0	36.0	41.0
19	39.318037372608195	40.0	39.0	41.0	36.0	41.0
20	39.3113647754178	40.0	39.0	41.0	36.0	41.0
21	39.24916719498658	40.0	39.0	41.0	36.0	41.0
22	39.14203043425927	40.0	39.0	41.0	36.0	41.0
23	39.1320787668149	40.0	39.0	41.0	36.0	41.0
24	39.08481588846735	40.0	39.0	41.0	36.0	41.0
25	39.03884735602031	40.0	39.0	41.0	36.0	41.0
26	38.955514856477386	40.0	38.0	41.0	36.0	41.0
27	38.86243625672376	40.0	38.0	41.0	35.0	41.0
28	38.63028475306615	40.0	38.0	41.0	35.0	41.0
29	38.39840764224995	40.0	38.0	41.0	34.0	41.0
30	38.28326012653603	40.0	38.0	41.0	34.0	41.0
31	38.59122136953257	40.0	38.0	41.0	35.0	41.0
32	38.596222292765944	40.0	38.0	41.0	35.0	41.0
33	38.67934558671708	40.0	38.0	41.0	35.0	41.0
34	38.78023649934161	40.0	38.0	41.0	35.0	41.0
35	38.837427244207504	40.0	38.0	41.0	35.0	41.0
36	38.816912361958416	40.0	38.0	41.0	35.0	41.0
37	38.76103051642552	40.0	38.0	41.0	35.0	41.0
38	38.70812515102976	40.0	38.0	41.0	35.0	41.0
39	38.65649700093172	40.0	38.0	41.0	35.0	41.0
40	38.541545956556185	40.0	38.0	41.0	34.0	41.0
41	38.599032469617434	40.0	38.0	41.0	35.0	41.0
42	38.573657650512054	40.0	38.0	41.0	34.0	41.0
43	38.554043261897206	40.0	38.0	41.0	34.0	41.0
44	38.50292205636362	40.0	38.0	41.0	34.0	41.0
45	38.30447493792809	40.0	38.0	41.0	34.0	41.0
46	38.11965210626067	40.0	38.0	41.0	33.0	41.0
47	38.067468954951366	40.0	38.0	41.0	33.0	41.0
48	38.09687613603942	40.0	38.0	41.0	33.0	41.0
49	37.990444610394	40.0	37.0	41.0	33.0	41.0
50	37.935665035772644	40.0	37.0	41.0	33.0	41.0
51	37.00275590467623	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	4.0
11	8.0
12	7.0
13	6.0
14	7.0
15	18.0
16	29.0
17	86.0
18	157.0
19	384.0
20	894.0
21	1617.0
22	2884.0
23	4619.0
24	7510.0
25	11492.0
26	17001.0
27	24071.0
28	33490.0
29	44925.0
30	59589.0
31	76691.0
32	98617.0
33	128047.0
34	171117.0
35	239203.0
36	358875.0
37	607727.0
38	1317037.0
39	3388644.0
40	1621.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.262578963342083	25.81499330132103	31.27705957645663	19.64536815888026
2	17.622751380984305	34.32628674324804	29.54622700747167	18.504734868295984
3	17.885380197811468	28.44148504437736	35.840253035679254	17.832881722131923
4	17.632271751393418	27.691907472914696	29.862764163749155	24.81305661194273
5	24.133608393207066	28.282898109903275	30.022002970719402	17.561490526170257
6	24.29565176789337	28.96774322651417	28.377647019229634	18.358957986362828
7	18.368372237993494	35.05021388997787	29.009265988537354	17.572147883491283
8	17.864959912873452	35.37458867603483	28.754262563932777	18.006188847158946
9	24.023775457515953	28.525152121268903	29.082957900255018	18.368114520960127
10	17.878740194245875	34.92370514624532	28.684299969286194	18.51325469022261
11	24.17961846357594	28.554410584468883	28.66375840574424	18.60221254621094
12	17.86632429716775	28.60719709683278	28.63728935043483	24.88918925556464
13	17.766769723160365	35.06347873728356	28.697489017464424	18.472262522091658
14	17.722048237958322	28.860199424472384	28.76243370987308	24.655318627696214
15	17.702100591218123	35.12774656285014	28.81652833576472	18.353624510167013
16	17.741740851272713	28.754671879221068	28.92409808892208	24.579489180584144
17	24.085112111457462	28.69194052133427	28.907467760357118	18.31547960685115
18	17.86323169276734	28.610350340535163	35.1481312028321	18.378286763865404
19	17.87374294155315	28.57476374820507	28.859769763651183	24.691723546590598
20	17.94673589660988	28.61118417312539	35.031831877356886	18.410248052907846
21	24.1764149480234	28.617762205256593	28.7726906317802	18.433132214939807
22	17.916222219661833	34.892298792094614	28.78336839385999	18.408110594383565
23	17.88842029846981	34.95234279291714	28.74719599950215	18.412040909110893
24	17.91623044268813	28.693820339695307	28.814674468519257	24.57527474909731
25	17.895764678273636	28.671383797966826	35.00211176369107	18.430739760068473
26	17.924908986703972	28.71183911634501	28.76954635496263	24.593705541988385
27	17.931941402422254	28.809595800145853	28.736486126684696	24.521976670747193
28	18.05485127200437	34.926233062676026	28.64345732466526	18.37545834065434
29	24.253768249021913	28.73991999492031	28.686574117037956	18.319737639019813
30	18.06477674306241	28.68336617148434	34.829866814286596	18.421990271166646
31	18.007225742906925	28.708591016522654	28.6909572684096	24.593225972160816
32	18.00665434717304	34.87627405108039	28.642716478688186	18.474355123058388
33	17.998906408458566	28.73367800977257	28.728977325110268	24.538438256658594
34	18.080103379935057	28.711505975136483	34.875598275444645	18.332792369483816
35	24.148672154174218	28.84725952126227	28.746916006200742	18.25715231836277
36	18.013129865896463	34.96193703425743	28.702811701651846	18.322121398194263
37	17.977666255965303	28.807127341581527	28.7516477838047	24.46355861864847
38	18.02076648715697	28.8816262326416	28.6925657273675	24.40504155283393
39	18.064252908364626	34.96191543694188	28.641935365025518	18.331896289667977
40	18.02122841932042	34.899574740134135	28.753004751191742	18.326192089353704
41	18.04203331857629	28.734179134347727	34.82575063829271	18.398036908783272
42	18.095309436701452	28.66974115158076	28.776205288209077	24.458744123508712
43	18.075719798992232	28.612920563422133	34.831456245029955	18.479903392555677
44	18.162771911664734	28.675024077002735	28.696314652874904	24.46588935845763
45	24.212216914141287	28.735587226161847	28.785130662475183	18.267065197221687
46	18.073664390551	28.8905611605127	34.79224291047639	18.243531538459905
47	18.058545562551654	34.91269381726948	28.81307954522884	18.21568107495003
48	18.196418459420553	28.792187107939714	34.85961067442518	18.151783758214552
49	24.24809855330988	28.708399148608	28.67255297962182	18.370949318460305
50	18.197104054941796	28.71016043494375	28.58497701963082	24.50775849048363
51	18.272235978345442	28.662054132151887	28.53896930521898	24.526740584283687
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.90794712222234
2	36.12748624928029
3	35.71826191994339
4	42.445328363336145
5	41.69509891937732
6	42.65460975425619
7	35.940520121484774
8	35.8711487600324
9	42.39188997847608
10	36.39199488446849
11	42.78183100978688
12	42.755513552732396
13	36.23903224525202
14	42.37736686565454
15	36.055725101385136
16	42.32123003185686
17	42.40059171830861
18	36.24151845663274
19	42.56546648814375
20	36.356983949517726
21	42.60954716296321
22	36.3243328140454
23	36.3004612075807
24	42.491505191785436
25	36.32650443834211
26	42.51861452869236
27	42.45391807316945
28	36.43030961265871
29	42.57350588804173
30	36.48676701422905
31	42.60045171506774
32	36.48100947023143
33	42.53734466511716
34	36.41289574941887
35	42.40582447253699
36	36.33525126409073
37	42.44122487461377
38	42.4258080399909
39	36.3961491980326
40	36.34742050867412
41	36.44007022735956
42	42.554053560210164
43	36.55562319154791
44	42.628661270122365
45	42.47928211136297
46	36.31719592901091
47	36.274226637501684
48	36.348202217635105
49	42.619047871770185
50	42.704862545425435
51	42.79897656262913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	26.0
2	23.0
3	54.0
4	85.0
5	227.5
6	370.0
7	771.0
8	1172.0
9	2252.5
10	3333.0
11	5738.0
12	8143.0
13	12952.5
14	17762.0
15	26116.5
16	34471.0
17	47141.5
18	59812.0
19	78313.5
20	96815.0
21	120054.0
22	143293.0
23	170084.0
24	196875.0
25	223154.0
26	274784.5
27	300136.0
28	321817.0
29	343498.0
30	360505.5
31	377513.0
32	388855.0
33	400197.0
34	409346.0
35	418495.0
36	423749.0
37	429003.0
38	428248.0
39	427493.0
40	421349.5
41	415206.0
42	404822.0
43	394438.0
44	378142.5
45	361847.0
46	338510.5
47	315174.0
48	291233.5
49	267293.0
50	258400.0
51	249507.0
52	399631.5
53	549756.0
54	349284.0
55	148812.0
56	128234.0
57	107656.0
58	94551.0
59	81446.0
60	71050.5
61	60655.0
62	52204.0
63	43753.0
64	37191.5
65	30630.0
66	26063.5
67	21497.0
68	18087.5
69	14678.0
70	12261.0
71	9844.0
72	8176.5
73	6509.0
74	5370.0
75	3378.5
76	2526.0
77	2018.5
78	1511.0
79	1142.0
80	773.0
81	559.5
82	346.0
83	271.0
84	196.0
85	133.5
86	71.0
87	54.5
88	38.0
89	29.0
90	20.0
91	14.5
92	9.0
93	7.5
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049890985694885474
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.5159825492216795E-5
16	0.0
17	0.0
18	0.0
19	4.547947647665038E-4
20	1.0611877844551756E-4
21	0.0065642044381298715
22	0.002577170333676855
23	4.547947647665038E-5
24	0.0
25	0.0
26	0.06803729680906898
27	0.07451054229424554
28	0.08732059483516874
29	0.6791298624003279
30	0.09832662814251814
31	0.016281652578640837
32	0.07886141221051177
33	0.024225401136562436
34	0.0019859371394804
35	0.015735898860921034
36	0.011096992260302694
37	0.01758539757097148
38	0.0012279458648695603
39	0.010081283952324167
40	0.027681841348787865
41	0.010035804475847518
42	0.0253623880484787
43	0.009353612328697762
44	0.029031065817595163
45	0.002273973823832519
46	0.018616265704442223
47	0.012143020219265652
48	0.009353612328697762
49	0.023270332130552778
50	0.031198920862982163
51	0.02642357583293387
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6596382.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	17.427579731200627
#Duplication Level	Relative count
1	100.0
2	9.263565233625826
3	1.3317718675922312
4	0.5991276158794276
5	0.40620739206933815
6	0.29701793988356895
7	0.2195104012853805
8	0.15105483799792935
9	0.11484693675498
10++	0.7665891591280685
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	359269	5.446455344763235	TruSeq Adapter, Index 4 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2913350	3.817884	4.4828944	1
AAAAA	2862065	3.6011858	3.7689846	3
GGAAG	718565	3.5128407	96.87403	5
GAGCA	726320	3.374974	91.71834	9
CGGAA	721895	3.3544126	91.986694	4
TCCAG	718665	3.2000139	85.77631	25
CTCCA	735245	3.1117704	81.305405	24
TCGGA	649155	3.041051	92.46857	3
TCTCG	658105	2.9542913	85.68689	41
AGAGC	632935	2.941044	91.38452	8
GAAGA	941705	2.9284093	62.201115	6
ATCGG	620640	2.9074686	92.14413	2
CACTG	642740	2.8619409	85.40792	31
GCACA	646505	2.8553834	86.52255	11
CTGAA	946660	2.82094	58.800365	19
GTCTG	595490	2.8124354	91.61408	17
AGCAC	636370	2.810621	86.544334	10
CCAGT	621575	2.7676992	85.07643	26
CACAC	651410	2.7346208	81.75737	12
GATCG	582860	2.7304833	91.6179	1
GTCAC	603900	2.6889975	85.48583	29
CTGAC	601415	2.6779325	84.72142	33
CTCGT	594390	2.6682694	84.87485	42
CGTCT	593895	2.6660469	86.78175	16
CACGT	596755	2.657183	86.42223	14
ATGCC	590800	2.630667	84.26386	47
GACCA	590940	2.6099725	82.88874	35
TGACC	576980	2.5691304	83.783905	34
ACGTC	574530	2.558221	86.04721	15
ACACG	577480	2.5505247	85.77392	13
CAGTC	571190	2.543349	84.826996	27
ACTCC	592005	2.505537	81.17766	23
TCTGA	817630	2.4563456	58.91027	18
AAGAG	752425	2.3398077	61.660877	7
TGAAC	767220	2.2862294	58.20269	20
ACCAA	788120	2.2141626	53.072483	36
ACTGA	729475	2.1737533	57.43794	32
CCAAT	764120	2.1642702	53.468758	37
GAACT	711660	2.1206663	57.968903	21
TCACT	736150	2.102079	54.987225	30
AACTC	728185	2.0624893	54.976334	22
ATCTC	709575	2.0261939	54.505707	40
CAATC	707790	2.004723	53.368027	38
AGTCA	661960	1.9725661	57.18139	28
GTATG	579765	1.83246	59.696526	45
TATGC	591585	1.7772552	56.664455	46
TCGTA	589065	1.7696846	56.837173	43
CGTAT	566735	1.7026001	56.75026	44
AATCT	839900	1.6050359	36.556973	39
>>END_MODULE