##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW207_CGATGT_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4768014	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.555379870948364	34.0	31.0	34.0	31.0	34.0
2	32.763304386270676	34.0	31.0	34.0	31.0	34.0
3	32.87492276658584	34.0	31.0	34.0	31.0	34.0
4	36.30647602964253	37.0	37.0	37.0	35.0	37.0
5	36.22936866376651	37.0	37.0	37.0	35.0	37.0
6	36.20759586695844	37.0	37.0	37.0	35.0	37.0
7	36.12909400014345	37.0	36.0	37.0	35.0	37.0
8	36.14255348243525	37.0	37.0	37.0	35.0	37.0
9	37.931231535813446	39.0	38.0	39.0	35.0	39.0
10	37.84188154648875	39.0	38.0	39.0	35.0	39.0
11	37.87545464421875	39.0	38.0	39.0	35.0	39.0
12	37.83536835252581	39.0	38.0	39.0	35.0	39.0
13	37.83490337905887	39.0	38.0	39.0	35.0	39.0
14	39.373565178290164	41.0	39.0	41.0	36.0	41.0
15	39.27120809628495	41.0	39.0	41.0	36.0	41.0
16	39.32722890494868	41.0	39.0	41.0	36.0	41.0
17	39.29386197272072	41.0	39.0	41.0	36.0	41.0
18	39.19524166665618	40.0	39.0	41.0	36.0	41.0
19	39.2940119303341	40.0	39.0	41.0	36.0	41.0
20	39.27475212950298	40.0	39.0	41.0	36.0	41.0
21	39.22545927927225	40.0	39.0	41.0	36.0	41.0
22	39.111703531071846	40.0	39.0	41.0	36.0	41.0
23	39.1043627388678	40.0	39.0	41.0	36.0	41.0
24	39.05712630038418	40.0	39.0	41.0	36.0	41.0
25	39.02095862134633	40.0	38.0	41.0	36.0	41.0
26	38.93657380200646	40.0	38.0	41.0	36.0	41.0
27	38.83646230904523	40.0	38.0	41.0	35.0	41.0
28	38.54342143290687	40.0	38.0	41.0	34.0	41.0
29	38.33533836100313	40.0	38.0	41.0	34.0	41.0
30	38.21690120876323	40.0	38.0	41.0	34.0	41.0
31	38.534346795122666	40.0	38.0	41.0	34.0	41.0
32	38.53558672436784	40.0	38.0	41.0	34.0	41.0
33	38.63160951289153	40.0	38.0	41.0	35.0	41.0
34	38.75398876765043	40.0	38.0	41.0	35.0	41.0
35	38.780894728916486	40.0	38.0	41.0	35.0	41.0
36	38.583183690316346	40.0	38.0	41.0	35.0	41.0
37	38.6745504522428	40.0	38.0	41.0	35.0	41.0
38	38.62948011478154	40.0	38.0	41.0	35.0	41.0
39	38.53954707347755	40.0	38.0	41.0	34.0	41.0
40	38.5070001891773	40.0	38.0	41.0	34.0	41.0
41	38.55915565684161	40.0	38.0	41.0	34.0	41.0
42	38.515704442143	40.0	38.0	41.0	34.0	41.0
43	38.505473767484744	40.0	38.0	41.0	34.0	41.0
44	38.45470944506455	40.0	38.0	41.0	34.0	41.0
45	38.22479401276926	40.0	38.0	41.0	33.0	41.0
46	37.968422911509904	40.0	38.0	41.0	33.0	41.0
47	37.91388259346554	40.0	37.0	41.0	33.0	41.0
48	37.990213535446834	40.0	37.0	41.0	33.0	41.0
49	37.86961741303612	40.0	37.0	41.0	33.0	41.0
50	37.831638497705754	40.0	37.0	41.0	33.0	41.0
51	36.85217094580678	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	6.0
12	5.0
13	3.0
14	7.0
15	7.0
16	23.0
17	58.0
18	123.0
19	335.0
20	753.0
21	1230.0
22	2119.0
23	3595.0
24	5765.0
25	8883.0
26	13019.0
27	18247.0
28	25387.0
29	34433.0
30	45011.0
31	58271.0
32	74495.0
33	96768.0
34	128159.0
35	178090.0
36	268531.0
37	449463.0
38	960985.0
39	2393190.0
40	1044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.340513528570575	24.75097337092173	29.05346694210974	19.855046158397954
2	17.43688252593218	36.07862728590982	28.694735376196462	17.78975481196154
3	17.315637076568986	26.928570260070543	37.923147876663116	17.832644786697355
4	17.474906743142952	26.210682267292	28.250693055850927	28.063717933714123
5	27.511685158642575	26.756255329787205	27.968059657542955	17.763999854027272
6	27.681651102534516	27.288384639810204	26.66453999505874	18.365424262596544
7	18.277526030754103	36.9820642305161	27.127017663958203	17.613392074771593
8	17.68237257692616	37.12459736905135	27.222864697964393	17.9701653560581
9	27.164454634571122	27.090377670870936	27.556441738635833	18.18872595592211
10	17.770711243716985	36.76337779209541	27.18714752095946	18.278763443228144
11	27.341530456915603	27.134316300245764	27.219362191470076	18.304791051368557
12	17.666621784248115	27.18052002364087	27.194613941989264	27.95824425012175
13	17.652674677549186	36.79949345786317	27.20772212497698	18.340109739610664
14	17.62108919982198	27.372046306911013	27.223116375077762	27.78374811818925
15	17.625116033635805	36.961741303611944	27.21116171219296	18.20198095055929
16	17.734406820114202	27.312692454342624	27.23712640105503	27.71577432448814
17	27.367537091963236	27.2027934481736	27.21617428136746	18.2134951784957
18	17.89015300710107	27.020222675520667	36.73881830044962	18.35080601692864
19	17.834273072834915	27.003780210856238	27.24228752253571	27.91965919377314
20	17.969869603853518	27.017671317315045	36.600476215544056	18.411982863287378
21	27.488436753045285	27.01869164367161	27.111210204014856	18.381661399268243
22	17.971865228943177	36.587471905917646	27.06803486980034	18.372627995338835
23	17.936320143825295	36.55380539500047	27.09045938900677	18.41941507216747
24	17.961125953069768	27.000780618513286	27.072424703450952	27.96566872496599
25	17.943550501319834	27.024878702117906	36.50847082244306	18.5230999741192
26	17.975709266744737	27.05262362585471	27.029726451168784	27.94194065623177
27	18.033813965261682	27.106792807186263	26.94666845212284	27.912724775429215
28	18.215805192940504	36.508103106477705	26.830346534695032	18.445745165886766
29	27.6250806645066	27.060724747363835	26.93624378756601	18.377950800563553
30	18.212903090359422	26.908191971783673	36.28541736647632	18.593487571380585
31	18.107147037489042	27.018488397606482	26.872889487049644	28.001475077854828
32	18.104510437652202	36.465643506540665	26.793115477665268	18.636730578141865
33	18.096788702516324	27.008351102081985	26.868529721011765	28.026330474389926
34	18.248430233314163	26.986312230148833	26.831737035084164	27.93352050145284
35	27.60139896665424	27.091879083736835	26.839636325726058	18.46708562388287
36	18.18859140095972	36.43864137537995	26.931755183443357	18.441012040216982
37	18.225204510828057	27.06070998616142	36.30875077903642	18.4053347239741
38	27.6028959202374	27.016543171917316	26.99915624097489	18.381404666870388
39	18.254862037668822	27.121443390525467	36.20119739980959	18.42249717199612
40	18.196818262587847	36.38908405310994	26.922963151619765	18.491134532682448
41	18.20201165512362	26.992814079110783	36.23691326113782	18.568261004627775
42	18.22769827722025	26.880952795528547	26.94913309276527	27.94221583448593
43	18.18288410133184	26.87622809564002	36.28646112128173	18.654426681746408
44	18.249582776948518	26.90237452626433	26.935458662137968	27.91258403464918
45	27.58839176969785	26.94173296254761	26.95167446081529	18.51820080693925
46	18.284365993586444	27.071741266001116	36.23290859474199	18.41098414567045
47	18.273038076721466	36.37038294030647	26.95093196481689	18.405647018155175
48	18.373838107453626	27.000290505910275	36.167797052780834	18.458074333855265
49	27.70496793362299	26.918030028754952	26.815089521738866	18.561912515883193
50	18.393942394677527	26.848398742558487	26.74249303053139	28.015165832232597
51	18.427324672120676	26.845920161014725	26.681280338946568	28.04547482791803
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	46.19555968696853
2	35.22663733789372
3	35.14828186326634
4	45.538624676857076
5	45.27568501266985
6	46.04707536513106
7	35.8909181055257
8	35.65253793298426
9	45.35318059049323
10	36.04947468694513
11	45.64632150828416
12	45.624866034369866
13	35.992784417159854
14	45.40483731801123
15	35.827096984195094
16	45.45018114460234
17	45.58103227045894
18	36.24095902402971
19	45.753932266608054
20	36.381852467140895
21	45.87009815231353
22	36.34449322428201
23	36.355735215992766
24	45.92679467803576
25	36.46665047543904
26	45.91764992297651
27	45.9465387406909
28	36.66155035882726
29	46.00303146507015
30	36.80639066174001
31	46.10862211534387
32	36.74124101579407
33	46.12311917690624
34	46.181950734767
35	46.06848459053711
36	36.6296034411767
37	36.63053923480216
38	45.98430058710779
39	36.67735920966494
40	36.6879527952703
41	36.7702726597514
42	46.16991411170618
43	36.837310783078244
44	46.1621668115977
45	46.1065925766371
46	36.695350139256895
47	36.67868509487665
48	36.83191244130889
49	46.26688044950618
50	46.40910822691012
51	46.472799500038704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	7.5
2	6.0
3	28.0
4	50.0
5	114.0
6	178.0
7	407.0
8	636.0
9	1191.0
10	1746.0
11	3017.5
12	4289.0
13	6979.0
14	9669.0
15	14056.5
16	18444.0
17	25548.0
18	32652.0
19	43004.5
20	53357.0
21	66516.0
22	79675.0
23	95429.5
24	111184.0
25	127300.0
26	159359.5
27	175303.0
28	190770.0
29	206237.0
30	220627.5
31	235018.0
32	244976.0
33	254934.0
34	264738.0
35	274542.0
36	281486.0
37	288430.0
38	292610.5
39	296791.0
40	297232.5
41	297674.0
42	295286.0
43	292898.0
44	284927.0
45	276956.0
46	262696.0
47	248436.0
48	234061.5
49	219687.0
50	213606.0
51	207525.0
52	391397.5
53	575270.0
54	352216.0
55	129162.0
56	110439.5
57	91717.0
58	80855.0
59	69993.0
60	61453.5
61	52914.0
62	45460.5
63	38007.0
64	32406.0
65	26805.0
66	22716.5
67	18628.0
68	15680.5
69	12733.0
70	10744.0
71	8755.0
72	7259.0
73	5763.0
74	4724.0
75	2986.0
76	2287.0
77	1796.0
78	1305.0
79	987.5
80	670.0
81	498.5
82	327.0
83	236.0
84	145.0
85	99.0
86	53.0
87	43.5
88	34.0
89	20.5
90	7.0
91	7.0
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049475525868841824
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.453004122890579E-4
20	1.048654639017419E-4
21	0.006753335875272179
22	0.0022231478347169284
23	1.4681164946243866E-4
24	0.0
25	0.0
26	0.06782698205164667
27	0.07533534926701138
28	0.08729001215180995
29	0.6784795514442701
30	0.10096446864459711
31	0.01694625896652149
32	0.08011721442093081
33	0.025545227006464326
34	0.002265094020277625
35	0.016379985461452085
36	0.012059528348700319
37	0.01902259515177598
38	0.0013632510307226447
39	0.010843088967440113
40	0.028796056387418327
41	0.010423627111833145
42	0.02602760814041234
43	0.00985735360676374
44	0.028921894944100416
45	0.0025377442264221542
46	0.01940011082182225
47	0.012856505874353556
48	0.009836380513983391
49	0.024811168759152135
50	0.03166937009832605
51	0.02611150051153373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4768014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.187489646341415
#Duplication Level	Relative count
1	100.0
2	12.328144193517906
3	1.959510535764906
4	0.8321128224511009
5	0.6189171312482283
6	0.47422753472550316
7	0.3797363696494378
8	0.2923320419540773
9	0.23740905225361428
10++	0.9915666635169612
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	411945	8.639760705400613	TruSeq Adapter, Index 2 (100% over 51bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACCG	585935	4.6220145	167.11206	31
GGAAG	684000	4.2699184	136.28952	5
GAGCA	690630	4.1133647	129.42252	9
CGGAA	667880	3.9778662	129.91469	4
TCCAG	686995	3.9376812	122.46284	25
CTCCA	701770	3.8376915	116.73252	24
TCGGA	624395	3.7510953	130.88385	3
CGATG	621890	3.7360463	127.06402	34
AGAGC	622155	3.70553	129.04918	8
TCTCG	633070	3.660038	122.8513	41
ATCGG	607485	3.6495073	130.60178	2
GTCTG	593395	3.5957499	130.205	17
TCACC	655660	3.5855348	116.35373	30
AGCAC	630380	3.5821395	123.0295	10
GAAGA	832690	3.5726733	94.06063	6
GCACA	623470	3.542873	122.89342	11
GATCG	586460	3.5231981	130.14026	1
CCAGT	614450	3.521871	121.8464	26
TTTTT	1651655	3.4950778	4.4049225	1
CCGAT	600890	3.4441488	121.259964	33
CGTCT	593940	3.4338114	124.06934	16
CTCGT	589970	3.4108596	122.05903	42
CACAC	628480	3.4073741	116.95964	12
ATGCC	593260	3.400416	120.82965	47
CACGT	592725	3.3973494	123.2801	14
GTCAC	591465	3.3901274	121.98324	29
CTGAA	819215	3.382541	89.617455	19
AAAAA	1659850	3.3641224	3.685223	13
ACCGA	590760	3.3569984	120.18774	32
ACGTC	579995	3.3243842	123.085365	15
CAGTC	573675	3.2881596	121.46029	27
ACACG	578585	3.287814	122.279755	13
ACTCC	591870	3.2366936	116.68693	23
TCTGA	735570	3.0634873	90.056625	18
AAGAG	700970	3.007526	93.42101	7
TGAAC	701540	2.8966606	88.964745	20
GATGT	647285	2.8255262	92.32848	35
GAACT	664135	2.7422152	88.813324	21
AACTC	676605	2.6654384	84.67586	22
ATCTC	670380	2.663799	84.496544	40
AGTCA	629480	2.5991247	87.799416	28
GTATG	576650	2.5171902	91.96637	45
TCGTA	601480	2.505032	87.87027	43
GTATC	590715	2.4601982	87.74413	38
TATGC	587410	2.4464338	87.600975	46
CGTAT	578960	2.411241	87.75867	44
TGTAT	659495	1.9957619	64.22498	37
ATGTA	654155	1.9625962	63.462585	36
TATCT	639350	1.8459682	61.02652	39
>>END_MODULE