##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW206_ATCACG_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6994302	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61269902271878	34.0	31.0	34.0	31.0	34.0
2	32.86266921273917	34.0	31.0	34.0	31.0	34.0
3	32.95922237844462	34.0	31.0	34.0	31.0	34.0
4	36.35236911417322	37.0	37.0	37.0	35.0	37.0
5	36.26871759326377	37.0	37.0	37.0	35.0	37.0
6	36.252799636046596	37.0	37.0	37.0	35.0	37.0
7	36.23137719818218	37.0	37.0	37.0	35.0	37.0
8	36.2068066263081	37.0	37.0	37.0	35.0	37.0
9	38.0123880839003	39.0	38.0	39.0	35.0	39.0
10	37.98669888717988	39.0	38.0	39.0	35.0	39.0
11	37.977363288002145	39.0	38.0	39.0	35.0	39.0
12	37.959976849727106	39.0	38.0	39.0	35.0	39.0
13	37.93262401308951	39.0	38.0	39.0	35.0	39.0
14	39.42747610840939	41.0	39.0	41.0	36.0	41.0
15	39.37662971944877	41.0	39.0	41.0	36.0	41.0
16	39.356734810707344	41.0	39.0	41.0	36.0	41.0
17	39.350660437596204	41.0	39.0	41.0	36.0	41.0
18	39.33680072722053	41.0	39.0	41.0	36.0	41.0
19	39.37699573166844	41.0	39.0	41.0	36.0	41.0
20	39.34957340989851	41.0	39.0	41.0	36.0	41.0
21	39.304975392826904	40.0	39.0	41.0	36.0	41.0
22	39.24440680428154	40.0	39.0	41.0	36.0	41.0
23	39.18080417459812	40.0	39.0	41.0	36.0	41.0
24	39.09539851153124	40.0	39.0	41.0	36.0	41.0
25	39.08671215512284	40.0	39.0	41.0	36.0	41.0
26	38.95774660573707	40.0	39.0	41.0	36.0	41.0
27	38.86003335286352	40.0	38.0	41.0	35.0	41.0
28	38.76221944662956	40.0	38.0	41.0	35.0	41.0
29	38.452771841993666	40.0	38.0	41.0	34.0	41.0
30	38.474512967841534	40.0	38.0	41.0	34.0	41.0
31	38.717927821818385	40.0	38.0	41.0	35.0	41.0
32	38.706674089851994	40.0	38.0	41.0	35.0	41.0
33	38.7280203228285	40.0	38.0	41.0	35.0	41.0
34	38.862262881985934	40.0	38.0	41.0	35.0	41.0
35	38.92273539232364	40.0	39.0	41.0	35.0	41.0
36	38.92501982327901	40.0	39.0	41.0	35.0	41.0
37	38.89732685262947	40.0	39.0	41.0	35.0	41.0
38	38.84773305470653	40.0	38.0	41.0	35.0	41.0
39	38.81556801522153	40.0	38.0	41.0	35.0	41.0
40	38.75863381363859	40.0	38.0	41.0	35.0	41.0
41	38.73856633585453	40.0	38.0	41.0	35.0	41.0
42	38.68590060880986	40.0	38.0	41.0	35.0	41.0
43	38.647400698454256	40.0	38.0	41.0	35.0	41.0
44	38.57935759708403	40.0	38.0	41.0	35.0	41.0
45	38.503508141341335	40.0	38.0	41.0	34.0	41.0
46	38.47922894950776	40.0	38.0	41.0	34.0	41.0
47	38.41807245383456	40.0	38.0	41.0	34.0	41.0
48	38.363884487687265	40.0	38.0	41.0	34.0	41.0
49	38.311344291396054	40.0	38.0	41.0	34.0	41.0
50	38.228382760710076	40.0	38.0	41.0	34.0	41.0
51	37.2308963782233	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	11.0
12	8.0
13	11.0
14	31.0
15	55.0
16	126.0
17	254.0
18	487.0
19	890.0
20	1686.0
21	2456.0
22	3769.0
23	5716.0
24	8375.0
25	11923.0
26	16853.0
27	23247.0
28	31585.0
29	42472.0
30	56182.0
31	73718.0
32	95689.0
33	125498.0
34	170143.0
35	237513.0
36	354508.0
37	594635.0
38	1314047.0
39	3820503.0
40	1899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.143186024362898	26.744172407311673	35.699635835357185	23.41300573296824
2	18.810954974492095	30.736905555407816	30.544291624811166	19.907847845288924
3	19.381090493375893	31.575030646374717	29.14907020028589	19.894808659963495
4	19.325202143115924	29.103890566921475	31.249408447047326	20.321498842915275
5	19.817888904425345	29.83631533210891	31.81572657285888	18.530069190606866
6	19.82725367020183	30.767659160270743	29.798856269002965	19.606230900524455
7	20.149430207617574	30.434845392721105	31.021136919738378	18.394587479922944
8	19.18673228579492	31.224230809593294	30.65615410944509	18.932882795166694
9	18.80783815168404	30.53059476127854	31.46734298862131	19.19422409841611
10	19.142925198254236	30.70871117661205	30.708339445451454	19.44002417968226
11	19.100647927412915	30.53592767369782	30.791993253937278	19.57143114495199
12	19.050135381629218	30.810165188749355	30.57480217468448	19.564897254936948
13	19.113429760396393	30.91728095240955	30.64497357992263	19.324315707271435
14	19.15540678683877	31.015360789396855	30.47307651285289	19.356155910911482
15	19.08426316164215	31.039694883063383	30.586926329460752	19.289115625833713
16	19.16265554447034	30.773435290612273	30.70063317254531	19.363275992372074
17	19.24010430204472	30.78501614599999	30.694928528965438	19.27995102298986
18	19.109326420277533	30.838602622534744	30.713286329357814	19.33878462782991
19	19.05920538771759	30.798499745291828	30.74715778424052	19.395137082750058
20	19.077110438467475	30.898777045800053	30.755331525287833	19.26878099044464
21	19.18155181495765	30.86626215438536	30.69699727128651	19.25518875937048
22	18.941234990408358	30.95851638614257	30.840445626327632	19.259802997121437
23	19.004340250349678	30.859984927720397	30.89505635805857	19.24061846387135
24	19.04287232664532	30.864881156118223	30.772677530938758	19.319568986297703
25	18.96992723505505	30.847938793606566	30.99245643096338	19.18967754037501
26	19.036310095593244	30.852607503413022	30.817797678372095	19.293284722621635
27	19.024306417046343	30.900631205058797	30.813005966357288	19.26205641153757
28	19.096312421418254	30.972549015118318	30.77628247270371	19.15485609075972
29	19.14705533854907	30.914154551264488	30.777337526003222	19.161452584183213
30	19.094299256882802	30.921100652721446	30.807590724216528	19.177009366179224
31	19.059363183089296	30.89823822034	30.773988167860118	19.268410428710585
32	19.070691865656265	30.990335744865956	30.751233430252523	19.187738959225257
33	19.073989512464156	30.979331971968215	30.772910540750072	19.173767974817558
34	19.025235690096086	31.01855810190871	30.796944071195583	19.159262136799622
35	19.050365967097637	30.96791630053274	30.869147726013374	19.112570006356254
36	19.033442620512957	31.035857433923297	30.774843672718426	19.155856272845316
37	19.020133719529326	31.014922790592585	30.84189249384563	19.123050996032458
38	19.078564438080438	30.98755467927331	30.775721866490713	19.15815901615554
39	19.12491342019816	30.991293689555	30.779755854968037	19.104037035278804
40	19.043900653078794	31.024053007144325	30.835144262982023	19.09690207679486
41	19.05849707795583	30.966533081921188	30.88341379065292	19.091556049470064
42	19.06880514245565	30.97121655521085	30.774962220003644	19.18501608232986
43	18.997159974021955	30.96267472050867	30.91576038542679	19.124404920042586
44	19.034095825279724	30.993899635379684	30.819505434980808	19.152499104359784
45	19.06788300232681	31.037576982500447	30.826671310566216	19.067868704606525
46	18.967370592420195	31.016434467694133	30.926100308526117	19.090094631359552
47	19.031496379375604	31.120768460923266	30.80555729131738	19.04217786838375
48	19.03976421783769	31.023222175099598	30.879761907145042	19.057251699917668
49	19.066394815395647	31.025424348116832	30.82811432569153	19.080066510795994
50	19.032390150332528	30.999038477078127	30.80887919150267	19.159692181086672
51	19.050134710908917	30.99482136342399	30.77392378933585	19.181120136331245
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.55619175733114
2	38.71880281978102
3	39.27589915333939
4	39.646700986031206
5	38.34795809503221
6	39.433484570726286
7	38.54401768754052
8	38.119615080961616
9	38.00206225010015
10	38.5829493779365
11	38.67207907236491
12	38.61503263656616
13	38.437745467667824
14	38.51156269775026
15	38.373378787475865
16	38.525931536842414
17	38.52005532503458
18	38.44811104810745
19	38.45434247046765
20	38.34589142891212
21	38.436740574328134
22	38.201037987529794
23	38.24495871422103
24	38.36244131294302
25	38.159604775430054
26	38.32959481821488
27	38.28636282858391
28	38.25116851217797
29	38.30850792273229
30	38.27130862306202
31	38.327773611799884
32	38.25843082488152
33	38.24775748728172
34	38.18449782689571
35	38.16293597345389
36	38.18929889335828
37	38.14318471556179
38	38.23672345423598
39	38.22895045547696
40	38.14080272987365
41	38.15005312742589
42	38.253821224785504
43	38.12156489406454
44	38.186594929639504
45	38.135751706933334
46	38.057465223779744
47	38.07367424775936
48	38.09701591775536
49	38.14646132619164
50	38.1920823314192
51	38.23125484724016
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	127.5
2	132.0
3	182.0
4	232.0
5	334.5
6	437.0
7	652.0
8	867.0
9	1451.5
10	2036.0
11	3253.5
12	4471.0
13	7282.5
14	10094.0
15	15449.0
16	20804.0
17	30717.5
18	40631.0
19	56999.0
20	73367.0
21	96724.5
22	120082.0
23	151323.5
24	182565.0
25	220751.5
26	299136.5
27	339335.0
28	380501.5
29	421668.0
30	456360.0
31	491052.0
32	518140.0
33	545228.0
34	560309.5
35	575391.0
36	574735.0
37	574079.0
38	561805.5
39	549532.0
40	528303.5
41	507075.0
42	481052.0
43	455029.0
44	424069.0
45	393109.0
46	362313.5
47	331518.0
48	300928.0
49	270338.0
50	242694.5
51	215051.0
52	191640.0
53	168229.0
54	145303.0
55	122377.0
56	106217.5
57	90058.0
58	77616.0
59	65174.0
60	56067.5
61	46961.0
62	40650.5
63	34340.0
64	29779.0
65	25218.0
66	22019.5
67	18821.0
68	16176.0
69	13531.0
70	11577.5
71	9624.0
72	7979.0
73	6334.0
74	5236.0
75	3343.5
76	2549.0
77	2003.5
78	1458.0
79	1156.0
80	854.0
81	700.5
82	547.0
83	441.5
84	336.0
85	265.0
86	194.0
87	162.0
88	130.0
89	117.0
90	104.0
91	85.0
92	66.0
93	47.5
94	29.0
95	25.5
96	22.0
97	19.0
98	16.0
99	12.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050155111975433715
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	3.288391036017604E-4
20	1.286761709746019E-4
21	0.007577596735171
22	0.0027307942951276627
23	7.148676165255662E-5
24	0.0
25	0.0
26	0.06999983701018343
27	0.07789197549662569
28	0.09087397141272996
29	0.6938362112473839
30	0.10362720969154607
31	0.016384765770765974
32	0.0809516088953551
33	0.025892505070556005
34	0.0022875763728818117
35	0.01697095721631694
36	0.011452179216739569
37	0.018686639495978298
38	0.0015298166993647116
39	0.010780203657205537
40	0.02912370669725156
41	0.010565743372247867
42	0.02637861504979339
43	0.010065336040679971
44	0.029981547837082245
45	0.002573523419492038
46	0.01968745415911409
47	0.012796130335807633
48	0.010408472496612243
49	0.025063258635386346
50	0.03244069243793019
51	0.02746521382691225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6994302.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	4.749353515926676
#Duplication Level	Relative count
1	100.0
2	2.7169677091997135
3	0.6727032102485235
4	0.34942903922707136
5	0.20819275091672812
6	0.13966699621800605
7	0.09886540181724024
8	0.05649451532413728
9	0.050740444318901075
10++	0.3264127552061265
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	409440	5.0040283	6.0480475	47
CCCCC	383860	4.347511	5.588203	45
>>END_MODULE