##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW204_GAGTGG_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7171554	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59527223806723	34.0	31.0	34.0	31.0	34.0
2	32.82710860156669	34.0	31.0	34.0	31.0	34.0
3	32.92640144660418	34.0	31.0	34.0	31.0	34.0
4	36.33799438726948	37.0	37.0	37.0	35.0	37.0
5	36.260245408456804	37.0	37.0	37.0	35.0	37.0
6	36.23979237972691	37.0	37.0	37.0	35.0	37.0
7	36.21480644222996	37.0	37.0	37.0	35.0	37.0
8	36.19160156919965	37.0	37.0	37.0	35.0	37.0
9	37.98700379304123	39.0	38.0	39.0	35.0	39.0
10	37.957152940631836	39.0	38.0	39.0	35.0	39.0
11	37.94490371264025	39.0	38.0	39.0	35.0	39.0
12	37.927936957596636	39.0	38.0	39.0	35.0	39.0
13	37.89723412805648	39.0	38.0	39.0	35.0	39.0
14	39.397399921969495	41.0	39.0	41.0	36.0	41.0
15	39.34197009462663	41.0	39.0	41.0	36.0	41.0
16	39.32844555029496	41.0	39.0	41.0	36.0	41.0
17	39.3208972560201	41.0	39.0	41.0	36.0	41.0
18	39.297377109619475	40.0	39.0	41.0	36.0	41.0
19	39.329960703077745	40.0	39.0	41.0	36.0	41.0
20	39.29914729778232	40.0	39.0	41.0	36.0	41.0
21	39.25058920284223	40.0	39.0	41.0	36.0	41.0
22	39.182584834472415	40.0	39.0	41.0	36.0	41.0
23	39.11493157549953	40.0	39.0	41.0	36.0	41.0
24	39.02255117928416	40.0	39.0	41.0	36.0	41.0
25	39.01112701654342	40.0	39.0	41.0	36.0	41.0
26	38.87847571112202	40.0	38.0	41.0	35.0	41.0
27	38.776495164088566	40.0	38.0	41.0	35.0	41.0
28	38.65763222308582	40.0	38.0	41.0	35.0	41.0
29	38.346855646628335	40.0	38.0	41.0	34.0	41.0
30	38.35633811026174	40.0	38.0	41.0	34.0	41.0
31	38.588103917226306	40.0	38.0	41.0	35.0	41.0
32	38.56238578138016	40.0	38.0	41.0	34.0	41.0
33	38.581245571043596	40.0	38.0	41.0	34.0	41.0
34	38.726552710890836	40.0	38.0	41.0	35.0	41.0
35	38.77693439943421	40.0	38.0	41.0	35.0	41.0
36	38.78127390520939	40.0	38.0	41.0	35.0	41.0
37	38.71487658044546	40.0	38.0	41.0	35.0	41.0
38	38.668622170313434	40.0	38.0	41.0	35.0	41.0
39	38.63071825158118	40.0	38.0	41.0	35.0	41.0
40	38.54019700611611	40.0	38.0	41.0	34.0	41.0
41	38.516607837018306	40.0	38.0	41.0	34.0	41.0
42	38.45992249378587	40.0	38.0	41.0	34.0	41.0
43	38.41607983430091	40.0	38.0	41.0	34.0	41.0
44	38.33301011189486	40.0	38.0	41.0	34.0	41.0
45	38.22833530919519	40.0	38.0	41.0	34.0	41.0
46	38.16683176338071	40.0	38.0	41.0	33.0	41.0
47	38.08376022825736	40.0	38.0	41.0	33.0	41.0
48	38.019988415342056	40.0	37.0	41.0	33.0	41.0
49	37.93895158566749	40.0	37.0	41.0	33.0	41.0
50	37.834777650701646	40.0	37.0	41.0	33.0	41.0
51	36.82021539543591	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	1.0
12	0.0
13	4.0
14	3.0
15	19.0
16	55.0
17	111.0
18	237.0
19	536.0
20	1313.0
21	2066.0
22	3355.0
23	5627.0
24	8829.0
25	12941.0
26	18781.0
27	25883.0
28	36050.0
29	47596.0
30	62980.0
31	81451.0
32	105382.0
33	137857.0
34	186194.0
35	263825.0
36	403301.0
37	689916.0
38	1473042.0
39	3602656.0
40	1538.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.141796963667048	27.54900359120404	31.213186880030403	22.09601256509851
2	19.260846393961476	30.194920096815835	30.41184658164744	20.13238692757525
3	19.491912073729072	29.340293610004192	31.01835390209709	20.149440414169646
4	19.786632018667085	28.56505019693082	30.425386185476675	21.22293159892542
5	20.738336488855833	29.458817433432138	30.438744517575966	19.36410156013606
6	20.828065995180403	30.09247368143641	29.05251776672113	20.026942556662057
7	20.19894154042485	30.77738799707846	29.809717670675003	19.21395279182169
8	19.514626815889553	30.948285406482334	29.869621005433412	19.667466772194704
9	20.115458936793896	29.631416008301688	30.293601637804024	19.959523417100396
10	19.45716367749584	30.53131859566281	29.96729857991727	20.044219146924082
11	20.316489285306922	29.634455795773135	29.884987270541362	20.16406764837858
12	19.50928627184568	29.632824350203595	29.84958350728447	21.00830587066625
13	19.491507698331493	30.59611626712983	29.865995570834436	20.046380463704242
14	19.457093957599707	29.911188007508553	29.858633149802678	20.77308488508906
15	19.398964213190645	30.717224009918077	29.93440890696896	19.949402869922316
16	19.477521887166994	29.88587968521188	29.918355212831138	20.71824321478999
17	20.307425698809492	29.80989894240495	29.969557504552014	19.913117854233544
18	19.529086722347763	29.719402517222903	30.739223325934656	20.01228743449467
19	19.571882031277017	29.626224704135577	29.894424172923816	20.90746909166359
20	19.58501846463274	29.654796983858994	30.674646533774858	20.08553801773341
21	20.499909288179254	29.58479923682144	29.789496979596187	20.12579449540312
22	19.660638465649907	30.440869632756684	29.78295994240414	20.115531959189266
23	19.652833815167735	30.455657360590475	29.828889176134982	20.062619648106804
24	19.669209769598055	29.606846716904034	29.773560932539866	20.950382580958046
25	19.65324391338335	29.647019321056494	30.63188257384662	20.06785419171354
26	19.647602541883305	29.657844869207995	29.8144659617043	20.880086627204403
27	19.719523149722598	29.640888653950302	29.706476708774666	20.933111487552434
28	19.77493962948934	30.474651363964604	29.63792208763752	20.112486918908537
29	20.588896516928514	29.674458079584774	29.680004751883303	20.056640651603402
30	19.830399715467053	29.57616958409251	30.410645083257016	20.18278561718342
31	19.80798072945678	29.593650048073155	29.627121348246284	20.97124787422378
32	19.78902425392115	30.441167009486904	29.615984117368026	20.15382461922392
33	19.83017554998236	29.604224145734538	29.620487168182386	20.945113136100716
34	20.594223148124907	29.656147073506073	29.655408024675868	20.094221753693155
35	19.816365008435476	30.473629386507785	29.652102956203713	20.057902648853027
36	20.62405213427113	29.645356146493064	29.623154734221064	20.107436985014743
37	19.81037697969114	29.705830626847163	30.439528147432938	20.04426424602876
38	20.637267458536705	29.720602337347344	29.607738337351808	20.034391866764146
39	20.614051418717885	29.68639895352429	29.597370422976166	20.10217920478166
40	19.865983505490544	30.464085219772436	29.58560362517672	20.0843276495603
41	19.837149826315553	29.642619620813015	30.41048769844994	20.10974285442149
42	19.84256056183778	29.62276630234208	29.591537284991908	20.94313585082823
43	19.823386462706104	29.618056456199156	30.394162546483667	20.16439453461107
44	19.84884733645144	29.626192653461114	29.627740920044165	20.89721909004328
45	20.606697229936945	29.666717005842553	29.652800524531425	20.07378523968908
46	19.8273048658232	29.67671823457774	30.445676289788192	20.050300609810865
47	19.84506997469665	30.510800224026134	29.66676949406688	19.97736030721034
48	19.878372606056853	29.652813309843474	30.42022225559854	20.048591828501134
49	20.68307431981492	29.644142860589902	29.59309472093893	20.07968809865625
50	19.936692705056856	29.615193304429894	29.57567680890838	20.87243718160487
51	19.942910902371786	29.563755885177407	29.583422542312952	20.909910670137855
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.23780952876556
2	39.39323332153673
3	39.641352487898715
4	41.009563617592505
5	40.10243804899189
6	40.85500855184246
7	39.41289433224654
8	39.18209358808426
9	40.07498235389429
10	39.50138282441992
11	40.4805569336855
12	40.51759214251193
13	39.537888162035735
14	40.230178842688765
15	39.34836708311296
16	40.19576510195698
17	40.220543553043036
18	39.54137415684244
19	40.479351122940606
20	39.67055648236615
21	40.625703783582374
22	39.77617042483917
23	39.71545346327454
24	40.6195923505561
25	39.721098105096885
26	40.52768916908771
27	40.65263463727503
28	39.887426548397876
29	40.64553716853192
30	40.01318533265047
31	40.779228603680565
32	39.94284887314507
33	40.77528868608308
34	40.68844490181806
35	39.874267657288506
36	40.731489119285875
37	39.8546412257199
38	40.671659325300844
39	40.71623062349954
40	39.95031115505084
41	39.946892680737044
42	40.78569641266601
43	39.987780997317174
44	40.74606642649472
45	40.68048246962602
46	39.87760547563406
47	39.82243028190699
48	39.92696443455799
49	40.76276241847117
50	40.80912988666173
51	40.85282157250964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	26.0
2	24.0
3	68.0
4	112.0
5	229.5
6	347.0
7	721.0
8	1095.0
9	2123.0
10	3151.0
11	5369.5
12	7588.0
13	11956.5
14	16325.0
15	24009.5
16	31694.0
17	43624.0
18	55554.0
19	73145.5
20	90737.0
21	112556.0
22	134375.0
23	160246.0
24	186117.0
25	213014.0
26	266796.5
27	293682.0
28	319396.5
29	345111.0
30	368552.0
31	391993.0
32	409848.5
33	427704.0
34	444303.0
35	460902.0
36	473967.0
37	487032.0
38	494857.5
39	502683.0
40	501952.0
41	501221.0
42	494594.5
43	487968.0
44	472030.5
45	456093.0
46	427886.5
47	399680.0
48	370693.5
49	341707.0
50	312298.5
51	282890.0
52	253629.5
53	224369.0
54	225460.5
55	226552.0
56	179970.5
57	133389.0
58	118327.0
59	103265.0
60	92618.0
61	81971.0
62	73469.5
63	64968.0
64	58426.0
65	51884.0
66	46831.5
67	41779.0
68	36987.5
69	32196.0
70	28210.5
71	24225.0
72	20552.5
73	16880.0
74	13910.0
75	8746.5
76	6553.0
77	5040.5
78	3528.0
79	2709.0
80	1890.0
81	1361.5
82	833.0
83	594.0
84	355.0
85	251.5
86	148.0
87	103.0
88	58.0
89	41.5
90	25.0
91	18.5
92	12.0
93	8.5
94	5.0
95	4.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052471193830514275
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.3943979226817507E-5
16	0.0
17	0.0
18	0.0
19	3.6254345989725515E-4
20	8.366387536090503E-5
21	0.006874381758821031
22	0.002858515741497589
23	4.183193768045252E-5
24	0.0
25	0.0
26	0.07270390768862647
27	0.0782954433585803
28	0.09247647023225371
29	0.6995276058717539
30	0.10503999551561627
31	0.017178982407439168
32	0.08353837954786368
33	0.026702720219355526
34	0.002426252385466246
35	0.01733236617893416
36	0.011698998571299888
37	0.019981722232029486
38	0.001268902109640393
39	0.011420118986763539
40	0.02981222758693583
41	0.011029687568412648
42	0.027051319700025965
43	0.010541648295474035
44	0.03131817734343212
45	0.002802739824590319
46	0.020386097629607195
47	0.013414108016198443
48	0.011169127360680823
49	0.02581030554883921
50	0.033409774227454746
51	0.02871065322801725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7171554.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.734883812641105
#Duplication Level	Relative count
1	100.0
2	6.02810834690891
3	0.9625314079023662
4	0.5240632851975592
5	0.3893199326283237
6	0.34514178424496783
7	0.36005190932435044
8	0.28826241820139714
9	0.2877101913466052
10++	1.2601816826352266
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC	52828	0.7366325345943152	TruSeq Adapter, Index 7 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2838875	3.5727818	3.7938952	14
TTTTT	2882360	3.540719	3.8331857	14
GAAGA	711605	2.0107007	9.735145	6
GAGCA	450090	1.8683181	13.129313	9
CTCCA	467075	1.8546945	12.591306	24
CTGAA	663315	1.8287055	9.278696	19
GGAAG	427665	1.8106575	13.483749	5
TCCAG	429440	1.7392795	12.673235	25
CGGAA	416105	1.7272468	13.31934	4
GTGGA	367535	1.5485595	12.360244	36
TCTCG	382955	1.5435164	12.11295	41
TCGGA	350475	1.4477884	12.918471	3
TCTGA	518965	1.4238315	8.84415	18
CACAC	354050	1.4127123	12.301166	12
AGAGC	334815	1.389813	12.742441	8
AGCAC	332570	1.3534849	12.469252	10
GCACA	332070	1.3514498	12.425301	11
AAGAG	474780	1.3415315	9.07844	7
AGTGG	311545	1.3126532	12.182248	35
ATCGG	313255	1.2940352	12.637236	2
TGAAC	456385	1.2582164	8.716836	20
GATCG	301480	1.2453934	12.493417	1
GGATC	298055	1.231245	11.875479	38
CACGA	301720	1.2279322	11.908948	31
CCAGT	303030	1.227305	12.091086	26
ACGAG	293585	1.2186676	12.04449	32
CTCGT	301135	1.2137375	11.75605	42
CGTCT	293110	1.1813923	12.133355	16
GAGTG	277750	1.1702623	12.098107	34
TGGAT	414145	1.1589218	8.351778	37
CACGT	284105	1.1506567	12.135171	14
GTCAC	282020	1.1422123	11.832567	29
ATCTC	423755	1.1398672	8.108361	40
AACTC	421290	1.1387383	8.482833	22
GTCTG	274795	1.1296749	12.362573	17
ATGCC	277320	1.1231768	11.595638	47
GAACT	402545	1.1097839	8.566016	21
ACTCC	275185	1.092724	11.914914	23
ACGTC	268595	1.0878395	12.08101	15
TCACG	268520	1.0875357	11.721568	30
ACACG	263865	1.073871	12.170203	13
CAGTC	254915	1.032434	11.856503	27
CGAGT	249650	1.0312873	11.751466	33
GATCT	368445	1.0108651	8.11469	39
AGTCA	340460	0.93862057	8.234989	28
TCGTA	290815	0.7978795	7.92621	43
TATGC	263805	0.72377497	7.799909	46
GTATG	247025	0.6912619	7.941355	45
CGTAT	249510	0.68455523	7.824402	44
>>END_MODULE