##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW203_CGTACG_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7572129	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5878375817422	34.0	31.0	34.0	31.0	34.0
2	32.824423619830036	34.0	31.0	34.0	31.0	34.0
3	32.91745399477478	34.0	31.0	34.0	31.0	34.0
4	36.331649262710656	37.0	37.0	37.0	35.0	37.0
5	36.25524512326718	37.0	37.0	37.0	35.0	37.0
6	36.23365568653149	37.0	37.0	37.0	35.0	37.0
7	36.20414258658298	37.0	37.0	37.0	35.0	37.0
8	36.18549182138867	37.0	37.0	37.0	35.0	37.0
9	37.97565070536965	39.0	38.0	39.0	35.0	39.0
10	37.94904986431161	39.0	38.0	39.0	35.0	39.0
11	37.93831510266135	39.0	38.0	39.0	35.0	39.0
12	37.92166681259656	39.0	38.0	39.0	35.0	39.0
13	37.89543482420862	39.0	38.0	39.0	35.0	39.0
14	39.38995743469241	41.0	39.0	41.0	36.0	41.0
15	39.33666278004508	41.0	39.0	41.0	36.0	41.0
16	39.3238106482338	41.0	39.0	41.0	36.0	41.0
17	39.31322511806125	41.0	39.0	41.0	36.0	41.0
18	39.27603755826136	40.0	39.0	41.0	36.0	41.0
19	39.315892003424665	40.0	39.0	41.0	36.0	41.0
20	39.29247441505553	40.0	39.0	41.0	36.0	41.0
21	39.240256472122965	40.0	39.0	41.0	36.0	41.0
22	39.16739981582459	40.0	39.0	41.0	36.0	41.0
23	39.10360032165326	40.0	39.0	41.0	36.0	41.0
24	39.015791727795445	40.0	39.0	41.0	36.0	41.0
25	39.00119081436674	40.0	38.0	41.0	36.0	41.0
26	38.87153163925232	40.0	38.0	41.0	35.0	41.0
27	38.76842061195735	40.0	38.0	41.0	35.0	41.0
28	38.62728672477714	40.0	38.0	41.0	35.0	41.0
29	38.333617929647	40.0	38.0	41.0	34.0	41.0
30	38.317875857635286	40.0	38.0	41.0	34.0	41.0
31	38.557329121043765	40.0	38.0	41.0	34.0	41.0
32	38.53119882136186	40.0	38.0	41.0	34.0	41.0
33	38.556216356060496	40.0	38.0	41.0	34.0	41.0
34	38.68658788037024	40.0	38.0	41.0	35.0	41.0
35	38.751977416126955	40.0	38.0	41.0	35.0	41.0
36	38.735249623982895	40.0	38.0	41.0	35.0	41.0
37	38.706824196999285	40.0	38.0	41.0	35.0	41.0
38	38.6409979280596	40.0	38.0	41.0	35.0	41.0
39	38.59787795479976	40.0	38.0	41.0	35.0	41.0
40	38.487382214434014	40.0	38.0	41.0	34.0	41.0
41	38.47626394109239	40.0	38.0	41.0	34.0	41.0
42	38.42470459761053	40.0	38.0	41.0	34.0	41.0
43	38.381207319632296	40.0	38.0	41.0	34.0	41.0
44	38.30110818238833	40.0	38.0	41.0	34.0	41.0
45	38.18880938240751	40.0	38.0	41.0	33.0	41.0
46	38.1070083460015	40.0	38.0	41.0	33.0	41.0
47	38.026316376807635	40.0	37.0	41.0	33.0	41.0
48	37.975714227795116	40.0	37.0	41.0	33.0	41.0
49	37.89172965753753	40.0	37.0	41.0	33.0	41.0
50	37.79005772352795	40.0	37.0	41.0	33.0	41.0
51	36.7756893734906	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	4.0
13	2.0
14	2.0
15	8.0
16	42.0
17	101.0
18	283.0
19	622.0
20	1436.0
21	2246.0
22	3741.0
23	6199.0
24	9539.0
25	14195.0
26	20282.0
27	28519.0
28	38678.0
29	51528.0
30	68002.0
31	87913.0
32	112985.0
33	147108.0
34	198842.0
35	282579.0
36	432014.0
37	733915.0
38	1551865.0
39	3777877.0
40	1599.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.770208131863225	27.70593818158227	30.981866930432577	21.54198675612193
2	19.30458395518618	30.599452280857868	30.41209414155517	19.683869622400778
3	19.05952737994823	29.124820773655603	31.825025696207764	19.990626150188408
4	19.388721454692597	28.321163044105564	30.254304964957672	22.035810536244167
5	21.396465908068922	29.081398375542733	30.06050213883044	19.46163357755791
6	21.530642174743722	29.530030457748406	28.81643986783638	20.122887499671492
7	20.09475802644144	31.169622176273016	29.44665892511868	19.288960872166864
8	19.431311854301477	31.329088556203942	29.578101482423243	19.66149810707134
9	20.888946820636576	29.284855025581315	29.901920582705337	19.92427757107677
10	19.43026855459013	31.029674745372137	29.55378863725116	19.986268062786568
11	21.030980850960145	29.304162673404004	29.583582107489185	20.08127436814666
12	19.40837246697725	29.306619049939584	29.528709825202398	21.756298657880762
13	19.397345185218054	31.033557405057415	29.586276197883056	19.98282121184148
14	19.39478315807879	29.5313246776435	29.572343524522626	21.50154863975508
15	19.35672252810273	31.135087635194804	29.57025692509993	19.937932911602534
16	19.439090379997488	29.466996666327265	29.60364251586311	21.49027043781214
17	21.072092142117494	29.433346949054883	29.589181589484276	19.905379319343343
18	19.50197890183857	29.297506685372106	31.162002126482523	20.038512286306798
19	19.528103990284322	29.315650153220535	29.499694404135607	21.656551452359533
20	19.593255311160508	29.249070372752893	31.1217028887943	20.035971427292303
21	21.234211273913473	29.213638664692805	29.42529743630855	20.12685262508517
22	19.644640044120866	30.850649926940672	29.41641811702383	20.08829191191464
23	19.64252297539277	30.844625741024018	29.441716675759228	20.071134607823986
24	19.64570334182104	29.218202700984097	29.388405823514102	21.747688133680764
25	19.656374052792813	29.251443022167212	31.018198448547295	20.073984476492672
26	19.668067285194258	29.27721344091599	29.373157682246152	21.6815615916436
27	19.72108939468572	29.248184762911976	29.30592713781925	21.72479870458306
28	19.786782463734	30.875778327677555	29.240799485130964	20.096639723457486
29	21.394153541087334	29.28621617366402	29.257200895298197	20.06242938995045
30	19.81263162709498	29.168808812053527	30.795468861809912	20.223090699041585
31	19.79896603556267	29.235542593575897	29.20337980097373	21.7621115698877
32	19.80086435559461	30.850997323719927	29.178550267449584	20.169588053235888
33	19.76419798080618	29.199250909712482	29.22531346062725	21.81123764885409
34	19.82439727109614	29.282361887475112	29.18591416932273	21.70732667210602
35	21.398324001980214	29.266009870435344	29.272653737498832	20.063012390085607
36	19.815431009444794	29.21424845874419	30.81363857354805	20.15668195826296
37	19.821095238875184	30.819635406457397	29.25521600817464	20.104053346492783
38	19.85789292894369	29.28519762911882	29.2195349811418	21.637374460795687
39	21.425526097861532	29.26143560361417	29.188475970472744	20.12456232805155
40	19.87323070679786	30.811131137605262	29.18327835294503	20.132359802651845
41	19.896662737367635	29.27224759206612	30.69392741693165	20.137162253634603
42	19.916728631213832	29.171807995810937	29.192005642131257	21.71945773084397
43	19.856879950698723	29.17974547924124	30.76415397615714	20.199220593902904
44	19.89939120848781	29.2038285766749	29.21591589865936	21.68086431617792
45	21.43192402698802	29.205454551217443	29.253288980173142	20.109332441621394
46	19.837668757668553	29.30167687423067	30.75435762617768	20.106296741923096
47	19.906886610694027	30.831391434830863	29.20971962300819	20.052002331466916
48	19.973624359099663	29.216232707907935	30.672585446774566	20.137557486217837
49	21.505581305400913	29.22362631091723	29.120855895270342	20.149936488411512
50	19.98087379465473	29.218165165708264	29.118187874609347	21.68277316502766
51	19.99445184565299	29.170015924523966	29.09416472866553	21.741367501157516
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.31219488798516
2	38.98845357758696
3	39.05015353013664
4	41.424531990936764
5	40.85809948562683
6	41.65352967441521
7	39.38371889860831
8	39.09280996137282
9	40.81322439171335
10	39.41653661737669
11	41.11225521910681
12	41.16467112485802
13	39.38016639705953
14	40.896331797833874
15	39.29465543970527
16	40.92936081780963
17	40.97747146146084
18	39.54049118814537
19	41.18465544264386
20	39.62922673845281
21	41.36106389899864
22	39.73293195603551
23	39.71365758321676
24	41.39339147550181
25	39.73035852928549
26	41.349628876837855
27	41.44588809926878
28	39.88342218719148
29	41.45658293103779
30	40.035722326136565
31	41.56107760545037
32	39.97045240883049
33	41.57543562966027
34	41.53172394320216
35	41.461336392065824
36	39.97211296770776
37	39.925148585367964
38	41.49526738973938
39	41.55008842591308
40	40.0055905094497
41	40.033824991002234
42	41.636186362057806
43	40.05610054460163
44	41.58025552466573
45	41.54125646860942
46	39.94396549959165
47	39.95888894216094
48	40.1111818453175
49	41.65551779381243
50	41.66364695968239
51	41.7358193468105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	25.5
2	31.0
3	57.5
4	84.0
5	201.0
6	318.0
7	630.5
8	943.0
9	1880.5
10	2818.0
11	5023.0
12	7228.0
13	11425.5
14	15623.0
15	23005.0
16	30387.0
17	42483.5
18	54580.0
19	72414.5
20	90249.0
21	113072.0
22	135895.0
23	163082.0
24	190269.0
25	218884.0
26	275729.0
27	303959.0
28	331904.5
29	359850.0
30	384695.5
31	409541.0
32	428317.0
33	447093.0
34	464436.5
35	481780.0
36	495181.5
37	508583.0
38	515530.0
39	522477.0
40	522111.0
41	521745.0
42	514133.5
43	506522.0
44	490009.5
45	473497.0
46	444015.5
47	414534.0
48	385070.5
49	355607.0
50	325866.5
51	296126.0
52	266576.0
53	237026.0
54	265863.0
55	294700.0
56	219510.0
57	144320.0
58	128871.0
59	113422.0
60	102832.5
61	92243.0
62	83945.5
63	75648.0
64	68638.5
65	61629.0
66	56456.5
67	51284.0
68	45966.0
69	40648.0
70	35664.0
71	30680.0
72	26174.0
73	21668.0
74	17877.5
75	11246.0
76	8405.0
77	6567.0
78	4729.0
79	3568.5
80	2408.0
81	1763.0
82	1118.0
83	788.5
84	459.0
85	346.5
86	234.0
87	169.0
88	104.0
89	69.5
90	35.0
91	24.5
92	14.0
93	10.5
94	7.0
95	4.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050210449399369715
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	4.886340420243764E-4
20	1.0565060368094626E-4
21	0.006708813333740089
22	0.002654471417483775
23	7.92379527607097E-5
24	0.0
25	0.0
26	0.0691219074582591
27	0.07609484730120156
28	0.08861444383739368
29	0.6861082266295252
30	0.10131892893002747
31	0.0167588270088901
32	0.07963414252451326
33	0.024827891865022374
34	0.0019941551444778607
35	0.016336224594166317
36	0.011463090499382671
37	0.018449236667785245
38	0.0012149819423308821
39	0.010815980551836874
40	0.028486044017475138
41	0.0106178856699351
42	0.025620271392629467
43	0.00954817330766552
44	0.02923880456870188
45	0.0023375196064409362
46	0.018964283360729854
47	0.012281882677910003
48	0.009891537769628594
49	0.024524146379439653
50	0.031827244358885065
51	0.026993729240481774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7572129.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	12.818673031585414
#Duplication Level	Relative count
1	100.0
2	6.862959168248316
3	1.0172945675238367
4	0.4630173953451915
5	0.390233524251026
6	0.31744965315686047
7	0.30513238266400167
8	0.3090515141844567
9	0.30289287893802735
10++	2.007155214404488
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC	109232	1.4425533426596404	TruSeq Adapter, Index 12 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2848615	3.5035067	3.7469194	6
TTTTT	2874275	3.4716158	3.7576442	7
GAAGA	802765	2.160066	17.486029	6
GAGCA	535420	2.074947	23.90576	9
GGAAG	511445	2.0355518	24.749317	5
CTCCA	551585	2.019343	22.242867	24
CTGAA	743910	1.9420257	16.575417	19
TCCAG	512785	1.9279854	22.756977	25
CGGAA	491190	1.9035398	24.04587	4
CACCG	345440	1.8773624	31.387686	31
TCTCG	460285	1.7243367	21.907295	41
TCACC	452840	1.6578393	21.565971	30
TCGGA	427010	1.6488353	23.76493	3
AGCAC	422275	1.5934447	22.900759	10
AGAGC	408750	1.5840548	23.547302	8
CACAC	426270	1.56623	22.309021	12
TCTGA	598765	1.5574632	16.17041	18
GCACA	406810	1.5350878	22.797997	11
ATCGG	395060	1.5254653	23.533772	2
GATCG	393685	1.5201558	23.419312	1
AAGAG	552970	1.4879218	16.73494	7
CGATC	385100	1.4479114	21.463522	38
CCAGT	379625	1.4273262	22.149923	26
TGAAC	542970	1.4174587	16.007782	20
CTCGT	375210	1.4056258	21.510443	42
CGTCT	367465	1.3766111	22.354752	16
GTCAC	360535	1.3555511	22.002382	29
ATGCC	359060	1.3500054	21.524124	47
CACGT	357340	1.3435383	22.42163	14
GTCTG	348055	1.339103	22.923763	17
ACTCC	353030	1.2924366	21.69525	23
ACGTC	343495	1.2914835	22.342281	15
ATCTC	507430	1.2851882	14.847871	40
AACTC	504375	1.2820864	15.46483	22
ACACG	336495	1.2697558	22.523375	13
GAACT	482960	1.2607987	15.833691	21
CAGTC	328275	1.2342589	21.91439	27
ACCGT	314315	1.1817715	21.540665	32
ACGAT	451480	1.1786182	15.168547	37
GATCT	450905	1.1728607	15.069438	39
AGTCA	423235	1.1048826	15.415156	28
CCGTA	279980	1.0526778	21.332863	33
TCGTA	369355	0.96073896	14.941746	43
TACGA	360760	0.94178754	14.944161	36
GTACG	243400	0.9398528	21.851473	35
CGTAC	247600	0.9309344	21.192932	34
TATGC	341965	0.8894941	14.825828	46
GTATG	324815	0.8676974	15.2254305	45
CGTAT	328475	0.8544049	14.784767	44
>>END_MODULE