FastQCFastQC Report
Wed 23 Jul 2014
LW202_GTTTCG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW202_GTTTCG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8528916
Filtered Sequences 0
Sequence length 51
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC 222887 2.613309827415348 TruSeq Adapter, Index 9 (97% over 36bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3079150 3.6362925 3.8350902 14
TTTTT 3136875 3.54688 3.870868 1
CACGT 767240 2.4767337 38.54087 14
GAAGA 1000635 2.428508 30.445347 6
GAGCA 709855 2.38931 41.16677 9
GGAAG 677120 2.3558493 42.64405 5
CTCCA 729470 2.2781196 37.53834 24
CGGAA 662180 2.2288399 41.244995 4
TCCAG 682990 2.2047656 38.68574 25
CTGAA 921460 2.1447942 28.619593 19
TTTCG 908090 2.0772395 27.187466 35
TCTCG 641395 2.0525696 37.746735 41
TCGGA 589300 1.9663625 40.667847 3
AGAGC 575645 1.9375707 40.75824 8
CACAC 611390 1.9260302 37.98773 12
AGCAC 589525 1.9196676 39.381947 10
GATCG 572465 1.9101876 40.260456 1
TTCGA 819490 1.890937 26.98858 36
GTTTC 823355 1.8834097 26.993305 34
GCACA 576780 1.878166 39.271095 11
ATCGG 560905 1.8716145 40.44851 2
CGATC 560020 1.8078051 36.91405 38
AAGAG 730775 1.7735668 29.719467 7
TCTGA 765800 1.7670497 28.05709 18
CTCGT 539365 1.7260568 37.25508 42
CGTCT 536730 1.7176243 38.215736 16
CCAGT 531955 1.7172083 38.04011 26
TCGAT 733025 1.6914228 26.827105 37
GTCTG 508605 1.6824107 39.34186 17
ATGCC 518335 1.6732414 37.40002 47
GTCAC 513380 1.6572461 37.97219 29
TGAAC 703640 1.6377953 28.162592 20
ACGTC 506565 1.6352466 38.46754 15
CGTTT 712085 1.6288815 26.990149 33
ACACG 497780 1.6209189 38.81778 13
TCACG 501685 1.6194936 37.7857 30
ACTCC 504240 1.574731 37.00371 23
CAGTC 480215 1.5501859 37.798195 27
ATCTC 680075 1.5181377 26.15598 40
AACTC 664345 1.4959732 27.080439 22
GAACT 641105 1.4922388 27.960363 21
GATCT 614940 1.4189469 26.817175 39
AGTCA 564560 1.3140721 27.428549 28
ACGTT 550395 1.2700121 26.891773 32
TCGTA 531685 1.2268398 26.770666 43
TATGC 488000 1.1260384 26.596786 46
GTATG 470720 1.1227293 27.508774 45
CGTAT 483430 1.1154934 26.649801 44
Produced by FastQC (version 0.10.1)