##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename LW202_GTTTCG_L003_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8528916 Filtered Sequences 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.581784015694375 34.0 31.0 34.0 31.0 34.0 2 32.809602885055966 34.0 31.0 34.0 31.0 34.0 3 32.90790904729276 34.0 31.0 34.0 31.0 34.0 4 36.32654724234592 37.0 37.0 37.0 35.0 37.0 5 36.24836966385881 37.0 37.0 37.0 35.0 37.0 6 36.22549289968385 37.0 37.0 37.0 35.0 37.0 7 36.1890494641992 37.0 37.0 37.0 35.0 37.0 8 36.17891804773315 37.0 37.0 37.0 35.0 37.0 9 37.96518408669988 39.0 38.0 39.0 35.0 39.0 10 37.94268791016349 39.0 38.0 39.0 35.0 39.0 11 37.92822710412437 39.0 38.0 39.0 35.0 39.0 12 37.90660759233647 39.0 38.0 39.0 35.0 39.0 13 37.89613287315762 39.0 38.0 39.0 35.0 39.0 14 39.37562370176937 41.0 39.0 41.0 36.0 41.0 15 39.324751117258046 41.0 39.0 41.0 36.0 41.0 16 39.31633574536318 41.0 39.0 41.0 36.0 41.0 17 39.291683609030734 40.0 39.0 41.0 36.0 41.0 18 39.259057891999404 40.0 39.0 41.0 36.0 41.0 19 39.30041766151759 40.0 39.0 41.0 36.0 41.0 20 39.27594643914889 40.0 39.0 41.0 36.0 41.0 21 39.224997291566716 40.0 39.0 41.0 36.0 41.0 22 39.13956275334404 40.0 39.0 41.0 36.0 41.0 23 39.053625103119785 40.0 39.0 41.0 36.0 41.0 24 38.989334166264506 40.0 38.0 41.0 36.0 41.0 25 38.96989289142958 40.0 38.0 41.0 36.0 41.0 26 38.85103769341848 40.0 38.0 41.0 35.0 41.0 27 38.75159469269014 40.0 38.0 41.0 35.0 41.0 28 38.56877966672435 40.0 38.0 41.0 34.0 41.0 29 38.28658378157318 40.0 38.0 41.0 34.0 41.0 30 38.23762070115358 40.0 38.0 41.0 34.0 41.0 31 38.49980255404086 40.0 38.0 41.0 34.0 41.0 32 38.4898509963048 40.0 38.0 41.0 34.0 41.0 33 38.52078505638934 40.0 38.0 41.0 34.0 41.0 34 38.646570091674015 40.0 38.0 41.0 35.0 41.0 35 38.64670152689978 40.0 38.0 41.0 35.0 41.0 36 38.67399010612838 40.0 38.0 41.0 35.0 41.0 37 38.65783635341232 40.0 38.0 41.0 35.0 41.0 38 38.52096831531698 40.0 38.0 41.0 34.0 41.0 39 38.48883011627738 40.0 38.0 41.0 34.0 41.0 40 38.32963051811039 40.0 38.0 41.0 34.0 41.0 41 38.36964556808861 40.0 38.0 41.0 34.0 41.0 42 38.32373035447881 40.0 38.0 41.0 34.0 41.0 43 38.287923342192606 40.0 38.0 41.0 34.0 41.0 44 38.20833890262256 40.0 38.0 41.0 33.0 41.0 45 38.07501246348305 40.0 38.0 41.0 33.0 41.0 46 37.979954896964635 40.0 37.0 41.0 33.0 41.0 47 37.88972185914365 40.0 37.0 41.0 33.0 41.0 48 37.85405601368333 40.0 37.0 41.0 33.0 41.0 49 37.75137836977173 40.0 37.0 41.0 33.0 41.0 50 37.65622067329541 40.0 37.0 41.0 33.0 41.0 51 36.64531788095932 39.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 0.0 11 2.0 12 1.0 13 0.0 14 10.0 15 19.0 16 62.0 17 128.0 18 364.0 19 836.0 20 1772.0 21 2946.0 22 4886.0 23 7887.0 24 11763.0 25 17375.0 26 24909.0 27 34208.0 28 46345.0 29 61272.0 30 79462.0 31 102744.0 32 131185.0 33 171191.0 34 229802.0 35 325301.0 36 501146.0 37 843851.0 38 1747807.0 39 4179826.0 40 1814.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.304454423454114 26.8327984083636 30.061868295841666 21.800878872340625 2 19.460702860715244 30.999648724409994 29.56056783769473 19.97908057718003 3 19.351345469928418 28.363698270682935 32.27853340330706 20.006422856081592 4 19.49198467894396 27.63258543055178 29.569713196847054 23.3057166936572 5 22.7472400947553 28.377041115189783 29.428499471679636 19.44721931837528 6 22.776458344765032 28.818163996456292 28.230398798628105 20.174978860150574 7 20.106365216869296 31.721416883458573 28.786635957019623 19.385581942652504 8 19.426501562449435 31.89320893768915 28.865719864048373 19.81456963581304 9 22.186934424022937 28.523812404765152 29.191599495176174 20.097653676035733 10 19.45126438107727 31.596207536807725 28.81444722869823 20.138080853416778 11 22.33289670105791 28.567147337363856 28.849398915407303 20.25055704617093 12 19.408797085116092 28.593141262031423 28.815127268224945 23.182934384627544 13 19.359072125930187 31.639765240975525 28.849422365046156 20.151740268048133 14 19.36314063827103 28.819406827315454 28.88023519049783 22.937217343915687 15 19.34524856382687 31.70829681052082 28.883107771257215 20.063346854395096 16 19.46385683714085 28.751297351269496 28.864054939689876 22.920790871899783 17 22.366113114491924 28.69796114770036 28.902547521865618 20.033378215942097 18 19.50393226993911 28.57203658706452 31.751596568661245 20.17243457433512 19 19.50298480197972 28.573150453389275 28.83969208596586 23.084172658665143 20 19.532392438700214 28.521413771335684 31.73466368011646 20.211530109847644 21 22.499747899356734 28.51035488735205 28.780454343987223 20.20944286930399 22 19.653094200731104 31.449786151791017 28.720992252875664 20.17612739460221 23 19.604901354311714 31.426250582137833 28.754876943214768 20.213971120335685 24 19.63140450673919 28.5060141288764 28.750464889090242 23.112116475294165 25 19.59407268168663 28.546241984327196 31.61352509510001 20.24616023888616 26 19.64708689997874 28.525857484992283 28.73468194338391 23.09237367164507 27 19.692925073483252 28.53674162642933 28.66561453120325 23.10471876888417 28 19.782413251700945 31.434614778441432 28.59626551892196 20.186706450935663 29 22.670613447698834 28.54587188404955 28.6131943246545 20.170320343597112 30 19.82157484128034 28.442570260290502 31.412637550877555 20.323217347551605 31 19.766679620831518 28.517131980737148 28.571344808831473 23.144843589599862 32 19.78570218254648 31.389507029593624 28.56539542725557 20.25939536060433 33 19.829404227142344 28.51142047116436 28.534665086376847 23.124510215316445 34 22.688977001598012 28.5366285198851 28.550956573451852 20.223437905065037 35 19.8539645929925 28.559343330846094 31.409503418402608 20.1771886577588 36 19.834564602556707 28.504415085458696 31.45236687816409 20.208653433820505 37 19.81267765666006 28.529998769838123 31.423271496492518 20.234052077009306 38 19.882534567847532 28.563455592323756 28.547122710128157 23.006887129700555 39 22.72831101898055 28.54957449547924 28.458650416498887 20.263464069041323 40 19.92344588065678 31.309806806225804 28.507349483968657 20.25939782914876 41 19.91100622278674 28.536228607558243 31.324686222298936 20.228078947356078 42 19.90248210314966 28.4353743092238 28.527919555651465 23.13422403197508 43 19.859349250487274 28.473216813081127 31.333440586936746 20.333993349494854 44 19.878565681728087 28.514030507528364 28.528984028466812 23.078419782276736 45 22.72650846376533 28.487297610072332 28.57631458919344 20.209879336968896 46 19.889000374799057 28.57847490089403 31.310239331947653 20.22228539235926 47 19.913025939776006 31.359865288121362 28.561896754508474 20.165212017594158 48 19.992145917548083 28.486093360861318 31.304862343075186 20.216898378515413 49 22.798612285509286 28.500757491709106 28.475073843420752 20.225556379360853 50 19.97577354930404 28.458972078827916 28.50715287833363 23.058101493534412 51 20.023528669452688 28.42160532797349 28.417007960599623 23.137858041974198 >>END_MODULE >>Per base GC content pass #Base %GC 1 43.10533329579474 2 39.43978343789527 3 39.35776832601001 4 42.79770137260116 5 42.19445941313058 6 42.95143720491561 7 39.49194715952179 8 39.241071198262475 9 42.28458810005867 10 39.58934523449405 11 42.58345374722884 12 42.591731469743635 13 39.51081239397832 14 42.30035798218672 15 39.408595418221964 16 42.384647709040635 17 42.39949133043402 18 39.67636684427423 19 42.58715746064487 20 39.74392254854786 21 42.709190768660726 22 39.82922159533332 23 39.8188724746474 24 42.74352098203336 25 39.84023292057279 26 42.73946057162381 27 42.79764384236742 28 39.969119702636604 29 42.840933791295946 30 40.14479218883194 31 42.911523210431376 32 40.04509754315081 33 42.95391444245879 34 42.91241490666305 35 40.0311532507513 36 40.04321803637721 37 40.046729733669366 38 42.88942169754809 39 42.991775088021875 40 40.18284370980554 41 40.13908517014282 42 43.03670613512474 43 40.19334259998213 44 42.95698546400482 45 42.936387800734224 46 40.111285767158314 47 40.07823795737016 48 40.209044296063496 49 43.02416866487014 50 43.03387504283845 51 43.161386711426886 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 11.5 2 16.0 3 37.5 4 59.0 5 148.0 6 237.0 7 574.0 8 911.0 9 1841.5 10 2772.0 11 4931.5 12 7091.0 13 11576.5 14 16062.0 15 24094.0 16 32126.0 17 45090.5 18 58055.0 19 76845.0 20 95635.0 21 120073.5 22 144512.0 23 172996.5 24 201481.0 25 231928.0 26 293027.0 27 323679.0 28 353467.5 29 383256.0 30 410106.5 31 436957.0 32 457961.5 33 478966.0 34 496269.0 35 513572.0 36 529059.0 37 544546.0 38 553110.0 39 561674.0 40 563402.5 41 565131.0 42 558521.0 43 551911.0 44 537067.5 45 522224.0 46 494995.0 47 467766.0 48 438877.5 49 409989.0 50 381572.0 51 353155.0 52 429348.5 53 505542.0 54 365367.5 55 225193.0 56 199280.5 57 173368.0 58 157570.0 59 141772.0 60 129943.5 61 118115.0 62 108340.0 63 98565.0 64 90822.0 65 83079.0 66 76657.0 67 70235.0 68 63404.0 69 56573.0 70 50061.0 71 43549.0 72 37421.0 73 31293.0 74 26145.5 75 16809.0 76 12620.0 77 9853.5 78 7087.0 79 5372.5 80 3658.0 81 2688.0 82 1718.0 83 1260.5 84 803.0 85 567.5 86 332.0 87 242.0 88 152.0 89 106.5 90 61.0 91 41.0 92 21.0 93 14.0 94 7.0 95 8.0 96 9.0 97 5.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05107331341990002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.924424158943529E-4 20 1.4069783311267223E-4 21 0.006753495989408267 22 0.002380138343489372 23 9.379855540844815E-5 24 0.0 25 0.0 26 0.06997372233470232 27 0.07698516435148382 28 0.08940174812367714 29 0.694449329785872 30 0.10206455310381765 31 0.01657889466844321 32 0.081417146094533 33 0.0259118509315838 34 0.0022628901492288116 35 0.016356123099348148 36 0.011666195328925738 37 0.018560389151446678 38 0.0012780053174401061 39 0.01081028351082365 40 0.02812784180310839 41 0.01059923676115464 42 0.0266856890137035 43 0.00987229795673917 44 0.029265149287435826 45 0.00241531280176754 46 0.019697696635774115 47 0.012733153896696837 48 0.010165418442390568 49 0.02450487260045708 50 0.031633562811499136 51 0.026931910221650677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 8528916.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 15.056330420356126 #Duplication Level Relative count 1 100.0 2 8.510807419561798 3 1.261575322632843 4 0.4962271971112083 5 0.40254586127213576 6 0.3434982314099325 7 0.2838828358759773 8 0.28615389856298507 9 0.29807697766977614 10++ 1.6703666062942502 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC 222887 2.613309827415348 TruSeq Adapter, Index 9 (97% over 36bp) >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position AAAAA 3079150 3.6362925 3.8350902 14 TTTTT 3136875 3.54688 3.870868 1 CACGT 767240 2.4767337 38.54087 14 GAAGA 1000635 2.428508 30.445347 6 GAGCA 709855 2.38931 41.16677 9 GGAAG 677120 2.3558493 42.64405 5 CTCCA 729470 2.2781196 37.53834 24 CGGAA 662180 2.2288399 41.244995 4 TCCAG 682990 2.2047656 38.68574 25 CTGAA 921460 2.1447942 28.619593 19 TTTCG 908090 2.0772395 27.187466 35 TCTCG 641395 2.0525696 37.746735 41 TCGGA 589300 1.9663625 40.667847 3 AGAGC 575645 1.9375707 40.75824 8 CACAC 611390 1.9260302 37.98773 12 AGCAC 589525 1.9196676 39.381947 10 GATCG 572465 1.9101876 40.260456 1 TTCGA 819490 1.890937 26.98858 36 GTTTC 823355 1.8834097 26.993305 34 GCACA 576780 1.878166 39.271095 11 ATCGG 560905 1.8716145 40.44851 2 CGATC 560020 1.8078051 36.91405 38 AAGAG 730775 1.7735668 29.719467 7 TCTGA 765800 1.7670497 28.05709 18 CTCGT 539365 1.7260568 37.25508 42 CGTCT 536730 1.7176243 38.215736 16 CCAGT 531955 1.7172083 38.04011 26 TCGAT 733025 1.6914228 26.827105 37 GTCTG 508605 1.6824107 39.34186 17 ATGCC 518335 1.6732414 37.40002 47 GTCAC 513380 1.6572461 37.97219 29 TGAAC 703640 1.6377953 28.162592 20 ACGTC 506565 1.6352466 38.46754 15 CGTTT 712085 1.6288815 26.990149 33 ACACG 497780 1.6209189 38.81778 13 TCACG 501685 1.6194936 37.7857 30 ACTCC 504240 1.574731 37.00371 23 CAGTC 480215 1.5501859 37.798195 27 ATCTC 680075 1.5181377 26.15598 40 AACTC 664345 1.4959732 27.080439 22 GAACT 641105 1.4922388 27.960363 21 GATCT 614940 1.4189469 26.817175 39 AGTCA 564560 1.3140721 27.428549 28 ACGTT 550395 1.2700121 26.891773 32 TCGTA 531685 1.2268398 26.770666 43 TATGC 488000 1.1260384 26.596786 46 GTATG 470720 1.1227293 27.508774 45 CGTAT 483430 1.1154934 26.649801 44 >>END_MODULE