##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW201_GTGGCC_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10825090	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56361877822725	34.0	31.0	34.0	31.0	34.0
2	32.79634571167538	34.0	31.0	34.0	31.0	34.0
3	32.89363081507867	34.0	31.0	34.0	31.0	34.0
4	36.31826931692947	37.0	37.0	37.0	35.0	37.0
5	36.240843263196886	37.0	37.0	37.0	35.0	37.0
6	36.219190325438404	37.0	37.0	37.0	35.0	37.0
7	36.19593971043197	37.0	37.0	37.0	35.0	37.0
8	36.17316807527697	37.0	37.0	37.0	35.0	37.0
9	37.95643602039336	39.0	38.0	39.0	35.0	39.0
10	37.92798415532804	39.0	38.0	39.0	35.0	39.0
11	37.9234264103116	39.0	38.0	39.0	35.0	39.0
12	37.89885691481549	39.0	38.0	39.0	35.0	39.0
13	37.862571673769	39.0	38.0	39.0	35.0	39.0
14	39.3585434393617	41.0	39.0	41.0	36.0	41.0
15	39.29501519155961	40.0	39.0	41.0	36.0	41.0
16	39.2958572168915	40.0	39.0	41.0	36.0	41.0
17	39.283841704780286	40.0	39.0	41.0	36.0	41.0
18	39.24603786204087	40.0	39.0	41.0	36.0	41.0
19	39.29111305310164	40.0	39.0	41.0	36.0	41.0
20	39.26725108059148	40.0	39.0	41.0	36.0	41.0
21	39.21695293064538	40.0	39.0	41.0	36.0	41.0
22	39.13219991704457	40.0	39.0	41.0	36.0	41.0
23	39.0726076180429	40.0	39.0	41.0	36.0	41.0
24	38.99077374876329	40.0	38.0	41.0	36.0	41.0
25	38.97680859928185	40.0	38.0	41.0	36.0	41.0
26	38.84728016118111	40.0	38.0	41.0	35.0	41.0
27	38.746851527331415	40.0	38.0	41.0	35.0	41.0
28	38.6011687662643	40.0	38.0	41.0	35.0	41.0
29	38.309312255140604	40.0	38.0	41.0	34.0	41.0
30	38.29252033932281	40.0	38.0	41.0	34.0	41.0
31	38.540431811652375	40.0	38.0	41.0	34.0	41.0
32	38.51709048146482	40.0	38.0	41.0	34.0	41.0
33	38.54927118388854	40.0	38.0	41.0	34.0	41.0
34	38.68302924040354	40.0	38.0	41.0	35.0	41.0
35	38.70027537877283	40.0	38.0	41.0	35.0	41.0
36	38.71641676882132	40.0	38.0	41.0	35.0	41.0
37	38.67585793743978	40.0	38.0	41.0	35.0	41.0
38	38.61698045928487	40.0	38.0	41.0	35.0	41.0
39	38.58484779341327	40.0	38.0	41.0	34.0	41.0
40	38.50436291984639	40.0	38.0	41.0	34.0	41.0
41	38.488671872474036	40.0	38.0	41.0	34.0	41.0
42	38.433610251739246	40.0	38.0	41.0	34.0	41.0
43	38.386267643040384	40.0	38.0	41.0	34.0	41.0
44	38.30752446399984	40.0	38.0	41.0	34.0	41.0
45	38.18545684146737	40.0	38.0	41.0	33.0	41.0
46	38.10007889079906	40.0	38.0	41.0	33.0	41.0
47	38.015308602515084	40.0	37.0	41.0	33.0	41.0
48	37.97328798190131	40.0	37.0	41.0	33.0	41.0
49	37.889038982585824	40.0	37.0	41.0	33.0	41.0
50	37.793228324198694	40.0	37.0	41.0	33.0	41.0
51	36.78208901727376	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	3.0
12	5.0
13	13.0
14	9.0
15	37.0
16	64.0
17	145.0
18	363.0
19	895.0
20	1996.0
21	3209.0
22	5353.0
23	8605.0
24	13682.0
25	20338.0
26	29287.0
27	40796.0
28	55486.0
29	75202.0
30	98261.0
31	127312.0
32	163514.0
33	214576.0
34	287658.0
35	406966.0
36	621737.0
37	1054599.0
38	2247145.0
39	5345750.0
40	2077.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.791987171340704	26.438434219616852	32.64120190119252	21.128376707849927
2	19.294980457437305	30.3508053974609	31.5078027064902	18.846411438611597
3	19.087693497236515	29.2309809895345	32.50782210586702	19.173503407361974
4	19.292652532219133	28.620519552262387	30.36436648563661	21.722461429881875
5	21.648060200885165	28.983038478202026	30.138890300219213	19.230011020693595
6	21.652993185276056	29.52653511425771	28.970207176106623	19.850264524359613
7	19.935788062732044	31.504744995191725	29.56247938816213	18.996987553914103
8	19.13823349274694	31.64933501707607	29.761082817787198	19.45134867238979
9	20.83178985116983	29.25016789698746	30.181264081869063	19.736778169973647
10	19.12775782926516	31.26198488880924	29.765544674455363	19.84471260747024
11	21.060360699079638	29.29418600676761	29.75478263922055	19.8906706549322
12	19.12465392897426	29.379912776706707	29.687069576326845	21.808363717992183
13	19.123610057745477	31.360284302486168	29.74701365069482	19.76909198907353
14	19.09814144732284	29.57191118041513	29.791733833159817	21.538213539102216
15	19.101393152389495	31.455507529267656	29.766967295422024	19.676132022920825
16	19.173946821689242	29.539735928292515	29.75806205768266	21.528255192335582
17	21.10890533011735	29.474202985841224	29.75564175447964	19.661249929561787
18	19.246241832631412	29.39097042149303	31.59597749302777	19.766810252847783
19	19.218976067168676	29.333371885902377	29.722109264016062	21.725542782912886
20	19.259087025431782	29.329528094811703	31.59741958417478	19.81396529558173
21	21.22717818029636	29.261678189303836	29.676548557903505	19.834595072496302
22	19.290682572363224	31.22032451220119	29.689478818132546	19.799514097303035
23	19.343733377724067	31.209454117760956	29.674611240820166	19.772201263694818
24	19.351441881776502	29.307599290167563	29.633250162354308	21.707708665701624
25	19.320227360696308	29.340716797735634	31.534472230715867	19.804583610852198
26	19.330307414272045	29.42013283846307	29.626194228875956	21.62336551838893
27	19.40598976529174	29.34786045341196	29.56606493642644	21.68008484486986
28	19.45436749360729	31.24975498009884	29.496586734086282	19.799290792207593
29	21.371185879428957	29.332498669871377	29.54229622987599	19.754019220823675
30	19.496182922339898	29.28891491648498	31.34405316435493	19.87084899682019
31	19.486655075694568	29.300598353599057	29.46394864592032	21.74879792478606
32	19.474034506633576	31.211964631897835	29.467883669013005	19.846117192455576
33	19.49446384913676	29.35085352053253	29.46408152490235	21.690601105428357
34	21.338958787766362	29.40751848248344	29.471085218410536	19.78243751133966
35	19.54723605554683	29.389012593201702	31.319329594485968	19.744421756765497
36	21.376029040881146	29.36062684903077	29.44665857470782	19.81668553538027
37	21.33758323711209	29.36666202537949	29.508580083038694	19.787174654469723
38	19.527948458387197	29.40413093366979	29.440001729336323	21.627918878606685
39	19.52607147361025	29.404180249528938	29.383845798154933	21.685902478705877
40	19.54267018193471	31.184275840860813	29.42824347604962	19.844810501154857
41	19.568001856610127	29.358359013853253	31.24486558977859	19.828773539758025
42	19.562943505211642	29.31104899250696	29.456489318175016	21.669518184106384
43	19.49221589813572	29.313226501469092	31.326659737035556	19.867897863359634
44	19.536066889251362	29.33710271015902	29.489385829612193	21.637444570977422
45	21.355997339442762	29.364071391619245	29.483824181509128	19.796107087428865
46	19.51096671115823	29.435233732229786	31.279126558505908	19.774672998106073
47	19.607307096216875	31.242063152934875	29.42879646332478	19.72183328752347
48	19.60078636516202	29.383916949580186	31.235426454541347	19.779870230716448
49	21.433015877122546	29.354039597172182	29.408407861776727	19.80453666392854
50	19.623456569374667	29.31046384074743	29.411658166657475	21.654421423220434
51	19.647087218415837	29.271151315023502	29.37864367976682	21.703117786793843
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	40.92036387919063
2	38.1413918960489
3	38.261196904598485
4	41.015113962101005
5	40.87807122157876
6	41.50325770963567
7	38.93277561664614
8	38.58958216513673
9	40.56856802114348
10	38.9724704367354
11	40.95103135401183
12	40.93301764696645
13	38.89270204681901
14	40.636354986425054
15	38.77752517531032
16	40.70220201402483
17	40.770155259679136
18	39.0130520854792
19	40.944518850081565
20	39.07305232101351
21	41.06177325279266
22	39.09019666966626
23	39.11593464141888
24	41.059150547478126
25	39.124810971548506
26	40.95367293266097
27	41.086074610161596
28	39.253658285814886
29	41.12520510025263
30	39.36703191916009
31	41.235453000480625
32	39.32015169908915
33	41.185064954565114
34	41.12139629910602
35	39.29165781231232
36	41.192714576261416
37	41.12475789158181
38	41.155867336993886
39	41.21197395231613
40	39.387480683089564
41	39.396775396368156
42	41.23246168931803
43	39.360113761495356
44	41.17351146022878
45	41.15210442687163
46	39.2856397092643
47	39.329140383740345
48	39.38065659587846
49	41.23755254105109
50	41.2778779925951
51	41.35020500520968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	43.5
2	44.0
3	110.5
4	177.0
5	375.0
6	573.0
7	1193.5
8	1814.0
9	3449.5
10	5085.0
11	8671.0
12	12257.0
13	19448.0
14	26639.0
15	38641.5
16	50644.0
17	69408.0
18	88172.0
19	115444.0
20	142716.0
21	175971.5
22	209227.0
23	248933.0
24	288639.0
25	329681.5
26	411053.5
27	451383.0
28	489592.5
29	527802.0
30	562111.5
31	596421.0
32	621882.0
33	647343.0
34	669621.0
35	691899.0
36	708366.5
37	724834.0
38	733485.5
39	742137.0
40	740180.5
41	738224.0
42	727495.0
43	716766.0
44	693426.0
45	670086.0
46	628456.0
47	586826.0
48	544459.0
49	502092.0
50	457979.5
51	413867.0
52	369450.0
53	325033.0
54	291007.0
55	256981.0
56	320001.0
57	383021.0
58	269845.0
59	156669.0
60	138603.5
61	120538.0
62	107849.5
63	95161.0
64	85491.0
65	75821.0
66	68604.0
67	61387.0
68	54719.5
69	48052.0
70	41660.0
71	35268.0
72	30041.0
73	24814.0
74	20497.0
75	12831.5
76	9483.0
77	7388.0
78	5293.0
79	4034.0
80	2775.0
81	1989.5
82	1204.0
83	857.0
84	510.0
85	382.5
86	255.0
87	194.0
88	133.0
89	94.5
90	56.0
91	33.0
92	10.0
93	9.0
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05140834856800267
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	4.064631333319169E-4
20	1.200913803026118E-4
21	0.006882159871188138
22	0.0026697237621119085
23	7.390238787853033E-5
24	0.0
25	0.0
26	0.06872922072703322
27	0.07549128921791874
28	0.08907085299059869
29	0.6859065374976098
30	0.10094142404358762
31	0.01592596458782329
32	0.08015637745275096
33	0.024748062140822848
34	0.002272498427264808
35	0.016535659287821164
36	0.01086365101814396
37	0.017949042455998057
38	0.0011824382060564853
39	0.01011538934087384
40	0.027399310305965124
41	0.009810541990874904
42	0.025699555384758925
43	0.00961654822269376
44	0.02902516283929279
45	0.002678961560596725
46	0.01906681607266083
47	0.01260959493177424
48	0.010263194116630901
49	0.02401827606052236
50	0.03082653354383197
51	0.027029798366572474
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.082509E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	15.367940004687123
#Duplication Level	Relative count
1	100.0
2	9.998269297334717
3	1.4410984192915657
4	0.5451713395638629
5	0.3969078112380293
6	0.31787238952347985
7	0.29133494865582094
8	0.2803738317757009
9	0.2671051113418715
10++	1.8420445367485867
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC	183604	1.6960967530062105	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	4303580	3.6414428	3.8551555	13
TTTTT	4444315	3.5536819	3.9218087	1
GTGGC	545840	2.2452128	39.594772	34
GAAGA	1164805	2.2123554	20.412155	6
GAGCA	787165	2.1857116	28.751776	9
GGAAG	746885	2.1253693	29.465801	5
CTCCA	811470	2.121185	26.58706	24
TCCAG	763835	2.0462523	27.169739	25
CGGAA	730095	2.0272462	28.771986	4
CGTGG	491840	2.023094	39.42574	33
CTGAA	1101665	2.0187554	19.3331	19
CACGT	748840	2.0060818	26.848217	14
GGCCA	473600	1.922491	38.619556	36
TGGCC	475020	1.90656	38.092567	35
TCTCG	692955	1.8354844	26.178923	41
TCGGA	637555	1.750374	28.23686	3
CCATC	665000	1.7383118	25.403042	38
AGAGC	620865	1.7239484	28.266777	8
CACAC	639520	1.6907296	26.890474	12
AGCAC	617415	1.6728258	27.559547	10
GCACA	610710	1.6546593	27.510569	11
TCTGA	891605	1.6154467	18.753553	18
ATCGG	581615	1.5967935	27.95068	2
AAGAG	822440	1.5620894	19.701384	7
GATCG	566900	1.5563942	27.749554	1
CCAGT	561365	1.5038514	26.526367	26
CTCGT	566710	1.5010893	25.69923	42
CGTCT	558215	1.478588	26.610931	16
ACGTG	531750	1.4598918	26.473843	32
TGAAC	795640	1.4579773	18.815023	20
GTCTG	530310	1.4395572	27.127737	17
GTCAC	534590	1.4321233	26.402872	29
GCCAT	534310	1.4313734	25.62264	37
ATGCC	532390	1.4262297	25.839851	47
ACACG	516435	1.3992304	27.161997	13
ACGTC	517325	1.3858718	26.836355	15
ACTCC	530040	1.3855262	26.006794	23
TCACG	516015	1.3823625	26.103456	30
CATCT	759675	1.343057	17.404318	39
AACTC	746585	1.3349341	18.233004	22
CAGTC	496695	1.3306057	26.335762	27
GAACT	719200	1.3179041	18.634735	21
ATCTC	744640	1.3164761	17.500216	40
AGTCA	619630	1.1354462	18.178583	28
TCGTA	571455	1.0353857	17.502941	43
TATGC	511755	0.92721885	17.34376	46
CGTAT	504965	0.91491646	17.374865	44
GTATG	487970	0.9060806	17.784706	45
>>END_MODULE