##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW200_GTGAAA_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9633780	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60726059760551	34.0	31.0	34.0	31.0	34.0
2	32.837749460751645	34.0	31.0	34.0	31.0	34.0
3	32.93928136204065	34.0	31.0	34.0	31.0	34.0
4	36.34627705843397	37.0	37.0	37.0	35.0	37.0
5	36.27159318564468	37.0	37.0	37.0	35.0	37.0
6	36.25270568769476	37.0	37.0	37.0	35.0	37.0
7	36.22504250667962	37.0	37.0	37.0	35.0	37.0
8	36.20880806910683	37.0	37.0	37.0	35.0	37.0
9	38.01038771904694	39.0	38.0	39.0	35.0	39.0
10	37.98922302564518	39.0	38.0	39.0	35.0	39.0
11	37.974207112888195	39.0	38.0	39.0	35.0	39.0
12	37.95358094122972	39.0	38.0	39.0	35.0	39.0
13	37.926578248621	39.0	38.0	39.0	35.0	39.0
14	39.42751983126042	41.0	39.0	41.0	36.0	41.0
15	39.374610485188576	41.0	39.0	41.0	36.0	41.0
16	39.363751922921224	41.0	39.0	41.0	36.0	41.0
17	39.35426727618858	41.0	39.0	41.0	36.0	41.0
18	39.32526121626194	40.0	39.0	41.0	36.0	41.0
19	39.361940692023275	41.0	39.0	41.0	36.0	41.0
20	39.3374815492984	40.0	39.0	41.0	36.0	41.0
21	39.28908974462776	40.0	39.0	41.0	36.0	41.0
22	39.21561764956227	40.0	39.0	41.0	36.0	41.0
23	39.151280598062236	40.0	39.0	41.0	36.0	41.0
24	39.070930725011365	40.0	39.0	41.0	36.0	41.0
25	39.055624894901065	40.0	39.0	41.0	36.0	41.0
26	38.93206986250465	40.0	38.0	41.0	36.0	41.0
27	38.834982011214706	40.0	38.0	41.0	35.0	41.0
28	38.710644316145896	40.0	38.0	41.0	35.0	41.0
29	38.402418469178244	40.0	38.0	41.0	34.0	41.0
30	38.39143908206332	40.0	38.0	41.0	34.0	41.0
31	38.63341979991239	40.0	38.0	41.0	35.0	41.0
32	38.620180655983425	40.0	38.0	41.0	35.0	41.0
33	38.64563265924694	40.0	38.0	41.0	35.0	41.0
34	38.79027276935948	40.0	38.0	41.0	35.0	41.0
35	38.819763581896204	40.0	38.0	41.0	35.0	41.0
36	38.83942813724208	40.0	38.0	41.0	35.0	41.0
37	38.81185588626686	40.0	38.0	41.0	35.0	41.0
38	38.76560799603063	40.0	38.0	41.0	35.0	41.0
39	38.7241204387063	40.0	38.0	41.0	35.0	41.0
40	38.639937802191874	40.0	38.0	41.0	35.0	41.0
41	38.60913078770742	40.0	38.0	41.0	35.0	41.0
42	38.51928869042058	40.0	38.0	41.0	34.0	41.0
43	38.48477783383054	40.0	38.0	41.0	34.0	41.0
44	38.41840046170869	40.0	38.0	41.0	34.0	41.0
45	38.330950571841996	40.0	38.0	41.0	34.0	41.0
46	38.29857594838163	40.0	38.0	41.0	34.0	41.0
47	38.21110986549412	40.0	38.0	41.0	34.0	41.0
48	38.10935364934636	40.0	38.0	41.0	33.0	41.0
49	38.03433480938946	40.0	37.0	41.0	33.0	41.0
50	37.94404117594547	40.0	37.0	41.0	33.0	41.0
51	36.936422359655296	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	12.0
11	16.0
12	10.0
13	12.0
14	16.0
15	27.0
16	58.0
17	135.0
18	320.0
19	755.0
20	1623.0
21	2704.0
22	4398.0
23	7170.0
24	11087.0
25	16908.0
26	24008.0
27	33402.0
28	46483.0
29	62476.0
30	82085.0
31	106438.0
32	137318.0
33	180155.0
34	242005.0
35	342310.0
36	524172.0
37	893575.0
38	1921008.0
39	4990556.0
40	2530.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.231711515603443	27.39070943902177	31.289240980578587	22.088338064796204
2	19.1700661630222	30.56542707016353	29.94910616601168	20.315400600802594
3	19.44532675647565	29.584379132593853	31.031017938960616	19.93927617196988
4	19.418203446622197	28.662778265644427	30.454971983997975	21.464046303735397
5	20.827297281025725	29.399726794674574	30.732225564627804	19.040750359671904
6	20.92645877319183	29.821004839221988	29.29841661320894	19.954119774377244
7	19.888340817415386	30.981535804222226	30.022950492952923	19.107172885409465
8	19.232928300210304	31.30827151959044	29.902270967366913	19.556529212832345
9	20.40122361108516	29.419376402616628	30.269520375179837	19.909879611118377
10	19.252017380509002	30.872762300986732	29.87234501929668	20.002875299207577
11	20.568177807672587	29.453329845605776	29.900184558916642	20.078307787804995
12	19.294908125367197	29.455603096603827	29.814849415286627	21.43463936274235
13	19.21967285945911	30.95977902754682	29.887344323827197	19.93320378916687
14	19.1978434217929	29.74394266840223	29.930909777885734	21.127304131919143
15	19.17460427522782	31.062120067317302	29.96176266862671	19.80151298882816
16	19.20144533090853	29.697335832871417	29.98924617336082	21.11197266285923
17	20.546234188449393	29.648289664077858	29.972544525617153	19.832931621855597
18	19.307800261164363	29.50500219020987	31.2443298476818	19.94286770094397
19	19.31795486941094	29.486599793309594	29.91320497601552	21.282240361263945
20	19.355799710476912	29.484716996682714	31.219831397331927	19.939651895508447
21	20.71234775416107	29.41204621065027	29.829803560721444	20.045802474467212
22	19.359314676824525	30.794138969648504	29.862559178734937	19.98398717479204
23	19.367456616690408	30.82658987017964	29.879121100411947	19.926832412718007
24	19.39041782046277	29.49636897421043	29.86057703835639	21.252636166970408
25	19.36317831629952	29.512475892121266	31.195449761152943	19.928896030426273
26	19.381607465335396	29.575745866138565	29.849676349435615	21.192970319090424
27	19.456951197329186	29.5436287863465	29.772048651312005	21.227371365012306
28	19.506581045848197	30.86393201203313	29.72383109317405	19.90565584894463
29	20.82399343374222	29.558247314269277	29.73901383204111	19.878745419947393
30	19.57379921521049	29.47172129882737	30.955356485070702	19.99912300089144
31	19.503533618979603	29.452856685907353	29.734111432360212	21.309498262752832
32	19.52157291415496	30.789566445257105	29.70707428438571	19.98178635620223
33	19.565905818897892	29.478758469544115	29.698947408203736	21.25638830335426
34	20.78181250280919	29.566890256491806	29.74649151098093	19.90480572971807
35	19.541724394076322	29.60327318954818	31.01519435601127	19.839808060364224
36	20.79616113866094	29.567093077625177	29.74897452912221	19.887771254591676
37	19.49047375266132	30.903698071224706	29.76115885779094	19.84466931832304
38	19.52603480649776	30.921712939660218	29.733846769156607	19.818405484685417
39	19.568212398207397	30.886172193276256	29.679791717772975	19.865823690743373
40	19.58091273942166	29.5820932932395	29.69893385711021	21.138060110228633
41	20.85881978848989	29.545691663143092	29.655836958386985	19.939651589980038
42	19.613563557592563	30.761330107543966	29.681737274677296	19.943369060186182
43	19.543968429525876	29.52674809541319	30.99407877129781	19.935204703763123
44	19.57462308351488	29.519397411255728	29.76501443746329	21.140965067766107
45	19.543324676079614	29.58041384579293	31.01902312130328	19.857238356824176
46	19.521291235816424	29.639060169996824	29.752828824687157	21.0868197694996
47	20.823139504728903	29.641175848915886	29.75354587435694	19.78213877199827
48	19.634904753293288	29.575706852460154	30.949298717067382	19.84008967717918
49	19.601547561089948	30.83018036917324	29.721028472146298	19.84724359759051
50	19.663306180092327	29.55599357841823	30.939077937530485	19.841622303958957
51	20.904939846116452	29.489487593719023	29.658970689485642	19.946601870678883
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.32004958039964
2	39.48546676382479
3	39.38460292844553
4	40.882249750357595
5	39.86804764069763
6	40.88057854756907
7	38.99551370282485
8	38.78945751304265
9	40.311103222203535
10	39.254892679716576
11	40.64648559547758
12	40.729547488109546
13	39.15287664862598
14	40.32514755371204
15	38.976117264055986
16	40.313417993767764
17	40.37916581030499
18	39.25066796210833
19	40.600195230674885
20	39.29545160598536
21	40.75815022862828
22	39.343301851616566
23	39.29428902940841
24	40.64305398743318
25	39.29207434672579
26	40.57457778442582
27	40.68432256234149
28	39.41223689479283
29	40.70273885368961
30	39.57292221610193
31	40.813031881732435
32	39.503359270357194
33	40.82229412225216
34	40.68661823252726
35	39.381532454440546
36	40.68393239325261
37	39.33514307098436
38	39.344440291183176
39	39.43403608895077
40	40.71897284965029
41	40.79847137846993
42	39.55693261777874
43	39.479173133288995
44	40.71558815128098
45	39.40056303290379
46	40.60811100531602
47	40.60527827672718
48	39.47499443047247
49	39.44879115868046
50	39.504928484051284
51	40.85154171679534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	33.0
2	30.0
3	78.5
4	127.0
5	271.5
6	416.0
7	880.5
8	1345.0
9	2605.5
10	3866.0
11	6867.5
12	9869.0
13	15674.0
14	21479.0
15	31416.5
16	41354.0
17	57983.0
18	74612.0
19	98017.5
20	121423.0
21	152136.0
22	182849.0
23	218190.5
24	253532.0
25	290780.0
26	365391.0
27	402754.0
28	437410.0
29	472066.0
30	502867.5
31	533669.0
32	556974.0
33	580279.0
34	601252.0
35	622225.0
36	637856.5
37	653488.0
38	660812.5
39	668137.0
40	668074.0
41	668011.0
42	658520.0
43	649029.0
44	628923.5
45	608818.0
46	572472.0
47	536126.0
48	503961.0
49	471796.0
50	482259.0
51	492722.0
52	398663.5
53	304605.0
54	267264.5
55	229924.0
56	202084.0
57	174244.0
58	153995.5
59	133747.0
60	118504.0
61	103261.0
62	91028.0
63	78795.0
64	69138.0
65	59481.0
66	53050.5
67	46620.0
68	40853.5
69	35087.0
70	30486.0
71	25885.0
72	21933.0
73	17981.0
74	14866.0
75	9307.5
76	6864.0
77	5330.5
78	3797.0
79	2911.0
80	2025.0
81	1493.5
82	962.0
83	675.0
84	388.0
85	263.0
86	138.0
87	102.5
88	67.0
89	54.0
90	41.0
91	30.5
92	20.0
93	12.5
94	5.0
95	4.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05164120417946019
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0380141543610089E-5
16	0.0
17	0.0
18	0.0
19	4.982467940932843E-4
20	6.228084926166054E-5
21	0.007016975683480421
22	0.0026157956689897423
23	6.228084926166054E-5
24	1.0380141543610089E-5
25	0.0
26	0.07146727452775546
27	0.07937694238398635
28	0.09360811644027578
29	0.6985420053187845
30	0.10435156293791223
31	0.016473284629709213
32	0.0833421564536454
33	0.02601263470828688
34	0.0022524907149633895
35	0.01645252434662199
36	0.01175032022736662
37	0.01881919661856509
38	0.0012352368436896006
39	0.011220933008642506
40	0.02809904315855251
41	0.010888768479246982
42	0.027496994949023124
43	0.010193298995825108
44	0.030444955147408387
45	0.0024912339704664214
46	0.020272416434670502
47	0.013681026554478098
48	0.010442422392871749
49	0.02456979503372508
50	0.03250022317304319
51	0.028348166555599155
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9633780.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	13.866008227220512
#Duplication Level	Relative count
1	100.0
2	8.720728114118325
3	1.4019730205679701
4	0.5436919762690421
5	0.3584719633891273
6	0.30547054431887477
7	0.25645847937218963
8	0.2655770030832008
9	0.25246912524862225
10++	1.87670616127249
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG	106515	1.1056407765176286	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3835890	3.5584726	3.7110236	14
TTTTT	3985770	3.5583336	3.8029275	13
TGAAA	1674790	2.3335145	10.128292	35
GAAGA	993785	2.1118379	14.749843	6
GAGCA	647270	2.0289617	20.582945	9
CTCCA	668130	1.9741794	18.952	24
GGAAG	610585	1.9638107	21.189184	5
CTGAA	926830	1.9048928	13.89027	19
CGGAA	606320	1.9005979	20.594719	4
TCCAG	616835	1.8700777	19.352114	25
CACGT	562430	1.7051364	19.082994	14
TCTCG	564485	1.6982846	18.450594	43
GAAAC	792330	1.6410022	13.06056	36
CACAC	548115	1.6320367	19.129173	12
TCGGA	516410	1.6063879	20.188265	3
AGAGC	495885	1.5544236	20.117125	8
GCACA	502775	1.5360205	19.640959	11
TCTGA	740890	1.5110943	13.419174	18
AGCAC	492560	1.5048126	19.585014	10
GTGAA	712790	1.503132	13.337605	34
AAGAG	688390	1.4628599	14.066878	7
ATCGG	463960	1.4432325	19.890316	2
AAACG	667975	1.3834493	12.808134	37
GATCG	439375	1.3667564	19.660181	1
CTCGT	454210	1.3665161	18.081757	44
CCAGT	447345	1.3562297	18.753609	26
TGAAC	657200	1.3507284	13.328516	20
CGTCT	447000	1.3448244	18.841164	16
ACGTG	423620	1.3177476	18.86655	32
GTCAC	427285	1.2954131	18.671888	29
GTCTG	415945	1.2839824	19.307196	17
CGATC	422810	1.2818463	17.706495	40
ACACG	407360	1.2445194	19.180506	13
ACGTC	409240	1.2407056	18.921518	15
CGTGA	398560	1.2397939	18.778065	33
ACTCC	419205	1.23866	18.318695	23
TCACG	406765	1.2332021	18.566828	30
AACGA	594140	1.2305292	12.549183	38
AACTC	611925	1.2257549	12.867359	22
ATCTC	614355	1.2212151	12.236258	42
GAACT	581830	1.1958221	13.136937	21
CAGTC	386975	1.1732041	18.490555	27
ACGAT	532175	1.0937673	12.308937	39
GATCT	526145	1.0731076	12.330287	41
AGTCA	503285	1.0343903	12.846619	28
TCGTA	440560	0.89855134	12.329308	45
CGTAT	389190	0.79377884	12.224575	46
GTATG	378655	0.7924038	12.508594	47
>>END_MODULE