FastQCFastQC Report
Wed 23 Jul 2014
LW199_GTCCGC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW199_GTCCGC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10364530
Filtered Sequences 0
Sequence length 51
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG 334097 3.2234650292873868 TruSeq Adapter, Index 1 (97% over 36bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3636305 3.5385218 3.8437088 13
AAAAA 3624585 3.4453661 3.718022 7
GCACA 1165435 3.068936 49.355145 11
CACGT 1106305 2.926925 49.434994 14
ACGTC 1084705 2.8697786 49.28051 15
GAGCA 961355 2.6108298 51.646446 9
GGAAG 928170 2.5996644 53.9132 5
GAAGA 1300995 2.5417206 38.23714 6
CTCCA 974255 2.499277 47.62727 24
TCCGC 673260 2.4760654 66.27257 35
TCCAG 926430 2.4510338 49.302223 25
CGGAA 893085 2.4254236 51.958218 4
CCGCA 652400 2.3881211 66.172386 36
CTGAA 1230320 2.3415973 36.53896 19
TCTCG 848950 2.256606 49.340023 43
TCGGA 807610 2.2036033 52.012238 3
GTCCG 580525 2.2018878 68.25729 34
CGTCC 594005 2.1845872 66.3313 33
AGAGC 794320 2.1571994 51.20716 8
CGCAC 585880 2.1446235 65.82079 37
AGCAC 809255 2.1310089 49.496098 10
GATCG 771980 2.106385 51.650932 1
ATCGG 766100 2.0903409 51.836075 2
CACAC 786925 2.0092692 47.70746 12
TCTGA 1032905 1.9751107 36.30529 18
CCAGT 740645 1.9595069 48.691864 26
CTCGT 733010 1.9484241 48.914856 44
GTCTG 704115 1.9302437 51.125347 17
CGTCT 725235 1.9277573 49.47733 16
AAGAG 973720 1.9023318 37.45284 7
GTCAC 709920 1.8782185 49.12649 29
TCACG 695815 1.8409013 48.82636 30
TGAAC 950315 1.8086798 35.89536 20
ACTCC 702635 1.8024845 47.161396 23
ACACG 682995 1.7985287 49.159794 13
CAGTC 667785 1.7667431 48.793804 27
CATCT 915685 1.6977829 34.16056 41
ATCTC 910300 1.6877985 34.389168 42
GAACT 874875 1.6650994 35.71342 21
AACTC 896685 1.6547755 34.45033 22
CACAT 877370 1.6191308 33.852093 39
ACATC 853850 1.5757262 33.855118 40
AGTCA 785935 1.4958249 35.43502 28
TCGTA 734435 1.4043792 35.19042 45
GTATG 654030 1.2898037 36.004116 47
CGTAT 673105 1.2871046 35.041855 46
Produced by FastQC (version 0.10.1)