##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW199_GTCCGC_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10364530	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54330297659421	34.0	31.0	34.0	31.0	34.0
2	32.77020106073309	34.0	31.0	34.0	31.0	34.0
3	32.87405082526656	34.0	31.0	34.0	31.0	34.0
4	36.30287895350778	37.0	37.0	37.0	35.0	37.0
5	36.22539767842825	37.0	37.0	37.0	35.0	37.0
6	36.20097939800454	37.0	37.0	37.0	35.0	37.0
7	36.16779207547279	37.0	37.0	37.0	35.0	37.0
8	36.15680315460518	37.0	37.0	37.0	35.0	37.0
9	37.92428223952268	39.0	38.0	39.0	35.0	39.0
10	37.89957084402283	39.0	38.0	39.0	35.0	39.0
11	37.891043202151955	39.0	38.0	39.0	35.0	39.0
12	37.86215978920414	39.0	38.0	39.0	35.0	39.0
13	37.840611489377714	39.0	38.0	39.0	35.0	39.0
14	39.3316810313637	41.0	39.0	41.0	36.0	41.0
15	39.25830172714055	40.0	39.0	41.0	36.0	41.0
16	39.27273933309084	40.0	39.0	41.0	36.0	41.0
17	39.25403862982692	40.0	39.0	41.0	36.0	41.0
18	39.20477957032301	40.0	39.0	41.0	36.0	41.0
19	39.26036723324647	40.0	39.0	41.0	36.0	41.0
20	39.238062507417126	40.0	39.0	41.0	36.0	41.0
21	39.18383448164075	40.0	39.0	41.0	36.0	41.0
22	39.105980782534274	40.0	39.0	41.0	36.0	41.0
23	39.04348359259899	40.0	39.0	41.0	36.0	41.0
24	38.96092143107309	40.0	38.0	41.0	36.0	41.0
25	38.93986258904167	40.0	38.0	41.0	35.0	41.0
26	38.81559366416036	40.0	38.0	41.0	35.0	41.0
27	38.71932301802397	40.0	38.0	41.0	35.0	41.0
28	38.539796208800595	40.0	38.0	41.0	34.0	41.0
29	38.25852238355237	40.0	38.0	41.0	34.0	41.0
30	38.21376762863343	40.0	38.0	41.0	34.0	41.0
31	38.470449600705486	40.0	38.0	41.0	34.0	41.0
32	38.43761386189244	40.0	38.0	41.0	34.0	41.0
33	38.487358519874995	40.0	38.0	41.0	34.0	41.0
34	38.608619686565625	40.0	38.0	41.0	35.0	41.0
35	38.59498819531614	40.0	38.0	41.0	35.0	41.0
36	38.63629185307968	40.0	38.0	41.0	35.0	41.0
37	38.617002893522425	40.0	38.0	41.0	35.0	41.0
38	38.5520533975009	40.0	38.0	41.0	34.0	41.0
39	38.49597762754317	40.0	38.0	41.0	34.0	41.0
40	38.41844830397519	40.0	38.0	41.0	34.0	41.0
41	38.39424855733931	40.0	38.0	41.0	34.0	41.0
42	38.27770173852553	40.0	38.0	41.0	34.0	41.0
43	38.27674115468815	40.0	38.0	41.0	34.0	41.0
44	38.206762680025044	40.0	38.0	41.0	33.0	41.0
45	38.100582660284644	40.0	38.0	41.0	33.0	41.0
46	38.068032993295404	40.0	38.0	41.0	33.0	41.0
47	37.944902470251904	40.0	37.0	41.0	33.0	41.0
48	37.781480491638305	40.0	37.0	41.0	33.0	41.0
49	37.69581215935503	40.0	37.0	41.0	33.0	41.0
50	37.645411321111524	40.0	37.0	41.0	33.0	41.0
51	36.60335432479813	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	6.0
13	6.0
14	12.0
15	26.0
16	62.0
17	164.0
18	417.0
19	941.0
20	2094.0
21	3414.0
22	5568.0
23	9047.0
24	13969.0
25	20796.0
26	29674.0
27	41507.0
28	56430.0
29	75672.0
30	99435.0
31	127432.0
32	163934.0
33	212808.0
34	285301.0
35	403130.0
36	616054.0
37	1037645.0
38	2155899.0
39	5001300.0
40	1778.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.57334904970552	26.932354570546202	29.764216770486296	20.730079609261978
2	19.136622692973056	31.797766034735776	29.373131246665306	19.692480025625862
3	19.409486006601362	27.800797527721954	32.74623161879989	20.0434848468768
4	19.78923308630493	27.417191131676976	28.86964483676539	23.923930945252703
5	23.700611605157203	28.12434331320378	28.678637622738318	19.496407458900695
6	23.861834545319468	28.501417816340926	27.793271860856212	19.843475777483395
7	19.954865295387247	32.484994495650064	28.205388956373323	19.35475125258936
8	19.42410316724444	32.64697000249891	28.32183417868442	19.607092651572238
9	23.03618205552977	28.563099339767454	28.61577900782766	19.784939596875112
10	19.25222851397989	32.477758277509935	28.522547573310124	19.747465635200054
11	23.023079676550697	28.498185638904992	28.616435091605695	19.86229959293861
12	19.18484485065893	28.450658158160575	28.657430679442292	23.707066311738206
13	19.19308449104783	32.34915620872341	28.682902167295577	19.77485713293319
14	19.19006457601068	28.667455253639098	28.668902497267123	23.473577673083103
15	19.21675174851151	32.422319198265626	28.63589569425724	19.725033358965625
16	19.286171201202563	28.654478302441117	28.63158290824572	23.427767588110605
17	23.1155488960908	28.553615069858452	28.623314322984257	19.70752171106649
18	19.323577624841647	28.427048790442015	32.43519966655507	19.814173918161266
19	19.32503564093084	28.447377236579367	28.6235943348094	23.603992787680394
20	19.390844920941827	28.379162759105103	32.36660260195359	19.863389717999482
21	23.228879047827096	28.337645766546583	28.513700337867302	19.919774847759015
22	19.47849466619247	32.18882070375268	28.486551773179116	19.84613285687573
23	19.452131187874024	32.183809677722806	28.494523386262276	19.869535748140894
24	19.48830289458374	28.33602681452994	28.46256414907381	23.71310614181251
25	19.465465390133467	28.36702677304229	32.25215229248215	19.9153555443421
26	19.532556578137118	28.37085513166084	28.452632048479902	23.64395624172214
27	19.57573337826401	28.343655963606153	28.32004804621552	23.76056261191432
28	19.65419465386391	32.134042278488515	28.282351786913964	19.92941128073361
29	23.391740434471117	28.38026544266754	28.327079766371593	19.900914356489746
30	19.687441478456876	28.272635758631875	32.0208250012821	20.019097761629144
31	19.655634277156217	28.325677759528777	28.275044823706285	23.743643139608718
32	19.6481253581776	32.097376993302106	28.245701023874965	20.008796624645324
33	19.652082525087	28.349121882581834	28.243417738377175	23.75537785395399
34	23.422900143791768	28.347963532100067	28.270862276857667	19.958274047250498
35	19.724659491324985	28.33917071680882	31.98007682282934	19.95609296903686
36	19.676227246910692	28.277545859347363	28.27580896388589	23.770417929856055
37	19.6683397678554	28.381998598227298	28.251230169900527	23.698431464016775
38	23.43102289800583	28.344710101775345	28.25761385419855	19.96665314602028
39	19.75560680866536	28.368847158196044	28.17451870441522	23.701027328723384
40	19.720643772603268	32.05773350451653	28.1776411366227	20.0439815862575
41	19.737514765904095	28.389007779491713	28.19056908146572	23.682908373138474
42	19.704303236308327	31.991742054231565	28.228165512904535	20.075789196555576
43	19.719450785635452	28.323972558194377	31.968701952572204	19.987874703597967
44	19.70761835660114	28.31436218950761	28.317218911577307	23.660800542313943
45	19.691625140181134	28.292714131599634	31.98000746603547	20.035653262183757
46	19.751291378739325	28.352663162258622	28.236282743814083	23.659762715187973
47	23.487881290617917	28.356594025913445	28.213838768387074	19.94168591508156
48	19.804038551963355	28.306569606484004	31.903744181363248	19.985647660189393
49	19.789569763932843	32.0267720284744	28.26100614272773	19.922652064865026
50	19.819552277008093	28.307899411150878	31.903602545953046	19.968945765887984
51	23.526433626336317	28.244463332227664	28.194018346070365	20.035084695365654
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.3034286589675
2	38.82910271859891
3	39.452970853478156
4	43.713164031557625
5	43.1970190640579
6	43.705310322802866
7	39.30961654797661
8	39.03119581881668
9	42.821121652404884
10	38.999694149179945
11	42.885379269489306
12	42.891911162397136
13	38.96794162398102
14	42.66364224909378
15	38.94178510747713
16	42.71393878931317
17	42.82307060715729
18	39.13775154300291
19	42.92902842861123
20	39.25423463894131
21	43.14865389558611
22	39.32462752306821
23	39.321666936014914
24	43.20140903639625
25	39.380820934475565
26	43.176512819859255
27	43.336295990178336
28	39.58360593459752
29	43.29265479096087
30	39.706539240086016
31	43.39927741676494
32	39.65692198282292
33	43.40746037904099
34	43.38117419104226
35	39.68075246036184
36	43.446645176766744
37	43.36677123187218
38	43.397676044026106
39	43.45663413738874
40	39.76462535886077
41	43.42042313904256
42	39.7800924328639
43	39.70732548923342
44	43.36841889891508
45	39.727278402364895
46	43.4110540939273
47	43.429567205699485
48	39.789686212152745
49	39.71222182879787
50	39.78849804289607
51	43.56151832170197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	32.5
2	35.0
3	67.0
4	99.0
5	210.0
6	321.0
7	687.0
8	1053.0
9	2093.0
10	3133.0
11	5456.5
12	7780.0
13	12903.5
14	18027.0
15	26729.5
16	35432.0
17	50160.5
18	64889.0
19	86736.0
20	108583.0
21	137784.5
22	166986.0
23	201191.5
24	235397.0
25	271166.5
26	344050.5
27	381165.0
28	418184.0
29	455203.0
30	489653.5
31	524104.0
32	550915.0
33	577726.0
34	603421.0
35	629116.0
36	650647.5
37	672179.0
38	684387.5
39	696596.0
40	698343.5
41	700091.0
42	692908.0
43	685725.0
44	665439.0
45	645153.0
46	608396.5
47	571640.0
48	533503.5
49	495367.0
50	455948.5
51	416530.0
52	391981.5
53	367433.0
54	484647.5
55	601862.0
56	408011.5
57	214161.0
58	191041.0
59	167921.0
60	152959.0
61	137997.0
62	125734.5
63	113472.0
64	103631.0
65	93790.0
66	85700.0
67	77610.0
68	69548.0
69	61486.0
70	53985.0
71	46484.0
72	39806.0
73	33128.0
74	27439.5
75	17563.5
76	13376.0
77	10433.5
78	7491.0
79	5718.0
80	3945.0
81	2917.5
82	1890.0
83	1339.0
84	788.0
85	585.0
86	382.0
87	276.0
88	170.0
89	116.5
90	63.0
91	41.0
92	19.0
93	14.5
94	10.0
95	6.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.048791406846234224
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	4.0522821584770363E-4
20	9.648290853516754E-5
21	0.006734507015754694
22	0.002547148785328423
23	4.824145426758377E-5
24	0.0
25	0.0
26	0.06834849240631269
27	0.07559435883730377
28	0.08732668051518014
29	0.679287917541847
30	0.10069921163815437
31	0.01649857735951365
32	0.0799360897213863
33	0.024989073310608394
34	0.002286644932283471
35	0.0166433017223164
36	0.011693728514462305
37	0.018688739383261953
38	0.001292870974371245
39	0.011076237899837233
40	0.02818265758312244
41	0.010883272082766899
42	0.02538465323560258
43	0.01034296779496996
44	0.02898346572396433
45	0.0025182039127678727
46	0.01892994665459987
47	0.012919061452858933
48	0.01025613317728831
49	0.024323341241715736
50	0.03185865639831232
51	0.026503854974610525
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.036453E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	16.403827687192155
#Duplication Level	Relative count
1	100.0
2	8.447202839165396
3	1.2029877529560353
4	0.5046262165606552
5	0.3989004520490796
6	0.31603431229676365
7	0.29374617815648557
8	0.28517381887176324
9	0.29546065001343
10++	2.553991576228276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG	334097	3.2234650292873868	TruSeq Adapter, Index 1 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3636305	3.5385218	3.8437088	13
AAAAA	3624585	3.4453661	3.718022	7
GCACA	1165435	3.068936	49.355145	11
CACGT	1106305	2.926925	49.434994	14
ACGTC	1084705	2.8697786	49.28051	15
GAGCA	961355	2.6108298	51.646446	9
GGAAG	928170	2.5996644	53.9132	5
GAAGA	1300995	2.5417206	38.23714	6
CTCCA	974255	2.499277	47.62727	24
TCCGC	673260	2.4760654	66.27257	35
TCCAG	926430	2.4510338	49.302223	25
CGGAA	893085	2.4254236	51.958218	4
CCGCA	652400	2.3881211	66.172386	36
CTGAA	1230320	2.3415973	36.53896	19
TCTCG	848950	2.256606	49.340023	43
TCGGA	807610	2.2036033	52.012238	3
GTCCG	580525	2.2018878	68.25729	34
CGTCC	594005	2.1845872	66.3313	33
AGAGC	794320	2.1571994	51.20716	8
CGCAC	585880	2.1446235	65.82079	37
AGCAC	809255	2.1310089	49.496098	10
GATCG	771980	2.106385	51.650932	1
ATCGG	766100	2.0903409	51.836075	2
CACAC	786925	2.0092692	47.70746	12
TCTGA	1032905	1.9751107	36.30529	18
CCAGT	740645	1.9595069	48.691864	26
CTCGT	733010	1.9484241	48.914856	44
GTCTG	704115	1.9302437	51.125347	17
CGTCT	725235	1.9277573	49.47733	16
AAGAG	973720	1.9023318	37.45284	7
GTCAC	709920	1.8782185	49.12649	29
TCACG	695815	1.8409013	48.82636	30
TGAAC	950315	1.8086798	35.89536	20
ACTCC	702635	1.8024845	47.161396	23
ACACG	682995	1.7985287	49.159794	13
CAGTC	667785	1.7667431	48.793804	27
CATCT	915685	1.6977829	34.16056	41
ATCTC	910300	1.6877985	34.389168	42
GAACT	874875	1.6650994	35.71342	21
AACTC	896685	1.6547755	34.45033	22
CACAT	877370	1.6191308	33.852093	39
ACATC	853850	1.5757262	33.855118	40
AGTCA	785935	1.4958249	35.43502	28
TCGTA	734435	1.4043792	35.19042	45
GTATG	654030	1.2898037	36.004116	47
CGTAT	673105	1.2871046	35.041855	46
>>END_MODULE