##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW197_CCGTCC_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9839031	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42074915710704	34.0	31.0	34.0	31.0	34.0
2	32.60051503039273	34.0	31.0	34.0	31.0	34.0
3	32.72964878350317	34.0	31.0	34.0	31.0	34.0
4	36.21644367214617	37.0	37.0	37.0	35.0	37.0
5	36.12818955443885	37.0	35.0	37.0	35.0	37.0
6	36.06819584164335	37.0	36.0	37.0	35.0	37.0
7	35.946489547598745	37.0	35.0	37.0	35.0	37.0
8	36.049892514821835	37.0	35.0	37.0	35.0	37.0
9	37.700524675651494	39.0	38.0	39.0	35.0	39.0
10	37.79984746465379	39.0	38.0	39.0	35.0	39.0
11	37.630727456799356	39.0	38.0	39.0	35.0	39.0
12	37.64121517657583	39.0	38.0	39.0	35.0	39.0
13	37.758759780307635	39.0	38.0	39.0	35.0	39.0
14	39.08868586754122	40.0	39.0	41.0	36.0	41.0
15	39.10353306133501	40.0	38.0	41.0	36.0	41.0
16	39.15594604793907	40.0	39.0	41.0	36.0	41.0
17	39.07870683606953	40.0	38.0	41.0	36.0	41.0
18	38.78521868667758	40.0	38.0	41.0	35.0	41.0
19	39.01851930337449	40.0	39.0	41.0	36.0	41.0
20	39.05694493695568	40.0	39.0	41.0	36.0	41.0
21	38.92363211377218	40.0	38.0	41.0	35.0	41.0
22	38.94463946703695	40.0	38.0	41.0	36.0	41.0
23	39.02568708239663	40.0	39.0	41.0	36.0	41.0
24	38.63845626667911	40.0	38.0	41.0	34.0	41.0
25	38.59292271769446	40.0	38.0	41.0	34.0	41.0
26	38.550875690908995	40.0	38.0	41.0	34.0	41.0
27	38.5414976332527	40.0	38.0	41.0	34.0	41.0
28	38.11260763382085	40.0	38.0	41.0	33.0	41.0
29	37.980220409916384	40.0	38.0	41.0	33.0	41.0
30	37.46823747175916	39.0	37.0	41.0	32.0	41.0
31	37.97418790529271	40.0	38.0	41.0	33.0	41.0
32	38.098892360436714	40.0	38.0	41.0	33.0	41.0
33	38.258986987641364	40.0	38.0	41.0	34.0	41.0
34	38.3377223834339	40.0	38.0	41.0	34.0	41.0
35	38.38081138274694	40.0	38.0	41.0	34.0	41.0
36	38.2074354679846	40.0	38.0	41.0	33.0	41.0
37	37.81211828685162	40.0	37.0	41.0	32.0	41.0
38	37.928110501938654	40.0	38.0	41.0	33.0	41.0
39	37.713559800756805	40.0	37.0	41.0	32.0	41.0
40	37.671354729952576	40.0	37.0	41.0	32.0	41.0
41	37.33140905847334	40.0	37.0	41.0	31.0	41.0
42	36.018672367228035	39.0	35.0	41.0	27.0	41.0
43	36.4356888396835	39.0	35.0	41.0	28.0	41.0
44	36.82472948809695	39.0	36.0	41.0	30.0	41.0
45	36.78573062733515	39.0	35.0	41.0	30.0	41.0
46	36.85868567748186	39.0	35.0	41.0	30.0	41.0
47	36.31818468708961	39.0	35.0	41.0	28.0	41.0
48	35.80453532466764	39.0	35.0	41.0	26.0	41.0
49	35.742523222053066	39.0	35.0	41.0	26.0	41.0
50	36.100574741557374	39.0	35.0	41.0	28.0	41.0
51	34.85828878880451	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	10.0
11	3.0
12	6.0
13	9.0
14	12.0
15	40.0
16	75.0
17	223.0
18	618.0
19	1447.0
20	3220.0
21	6090.0
22	10367.0
23	16704.0
24	25759.0
25	37416.0
26	52057.0
27	69828.0
28	90256.0
29	116350.0
30	145931.0
31	179818.0
32	220786.0
33	275121.0
34	354066.0
35	483020.0
36	715914.0
37	1135418.0
38	2030154.0
39	3866969.0
40	1338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.044160974530925	21.728328151731535	23.49112687764217	18.73638399609537
2	16.522938082012345	42.71038479297402	23.579405329650857	17.187271795362776
3	16.92404465439737	22.4461738152873	43.60365365247858	17.02612787783675
4	16.749555926798077	21.943166964307768	23.445072995501285	37.86220411339287
5	37.28688323067587	22.600274356285695	23.30528280681299	16.80755960622545
6	37.456391793053605	23.069558374193555	22.25836060481972	17.21568922793312
7	17.314631898202173	43.33899344356167	22.760656003624746	16.585718654611416
8	16.786764875524835	43.4605196385701	22.845410284813617	16.90730520109145
9	36.99025849191857	22.833905086791574	23.116229636841272	17.05960678444859
10	16.906024587177335	43.1776259267808	22.837421693254143	17.078927792787724
11	37.06486949781945	22.87927540832019	22.85665122917084	17.199203864689522
12	16.883634170885323	22.884296227951715	22.78031241084615	37.45175719031681
13	16.831261127239056	43.141738246378125	22.808770497826465	17.218230128556357
14	16.8106696685883	23.101837975711227	22.83583617126524	37.251656184435234
15	16.825406892203105	43.16123203595964	22.791634664023317	17.22172640781394
16	16.907152747054056	23.084112652963487	22.820438313488392	37.188296286494065
17	36.99496423987281	22.93932197184865	22.902641530451525	17.163072257827018
18	17.01242734167623	22.824595226908016	42.93449222794399	17.228485203471763
19	16.906049518745895	22.81761698696237	22.98820375809243	37.28812973619931
20	17.008191264616045	22.775758719010373	42.93439650176471	17.281653514608877
21	37.085514896291755	22.825480079375332	22.86102476225364	17.22798026207927
22	17.089562288877854	42.95739468095432	22.75257285063872	17.2004701795291
23	16.961673758660535	42.98631501136951	22.75691026014864	17.295100969821313
24	17.02466431907776	22.88694892820238	22.81570207472667	37.27268467799319
25	16.992039155075332	22.78203005966746	42.74355879151108	17.48237199374613
26	16.956248291393834	22.874229499980473	22.889596785216813	37.27992542340888
27	17.05237610616679	22.921205875634133	22.669790232351218	37.356627785847856
28	17.194758757122262	42.762113513063746	22.66172084911113	17.38140688070287
29	37.001040562925006	23.015868712489834	22.788041598372587	17.19504912621258
30	17.334031228254368	22.751130264223423	42.49433341878985	17.420505088732355
31	17.118518812179097	22.89133124134054	22.80406322331566	37.1860867231647
32	17.09067548134675	42.68745757250334	22.62004756558475	17.60181938056516
33	17.085674185145685	22.909173678929015	22.64214298763091	37.36300914829439
34	17.14579535457054	22.81894842802479	22.647617115527535	37.387639101877134
35	17.20691539241523	22.841237207718443	22.707188582436043	37.244658817430285
36	36.9638690693643	22.830770907167373	22.762524926453114	17.44283509701521
37	17.305210785939188	22.86830791212978	42.405863138011284	17.420618163919748
38	17.09756093098855	22.88075782316714	22.868957745150432	37.15272350069388
39	17.118562233940906	22.91748153087404	22.683268211960918	37.28068802322414
40	17.306073517893932	22.935293045998094	22.612817605776417	37.14581583033156
41	36.76044705991174	22.95381556618661	22.740887299369643	17.544850074532008
42	17.68633706124366	41.54606109218521	22.820006079330177	17.94759576724095
43	17.15811707353765	23.174864226694407	42.15298904563792	17.51402965413002
44	17.13590593058719	22.89551438542356	23.097236352124288	36.87134333186496
45	17.216912526245544	22.80038020842756	42.23966425221576	17.743043013111134
46	17.26790727849256	22.890529389179466	23.006822135022993	36.83474119730498
47	36.54978146093005	22.974611573346113	22.78269931302933	17.69290765269451
48	17.563585525968055	23.027177638581804	42.06090655013453	17.3483302853156
49	17.358143083029827	41.94778005562391	23.206460489400087	17.48761637194618
50	17.323263485506494	23.110589494999896	42.197682678403844	17.368464341089766
51	36.56567535777498	22.753075538123863	22.989237014422827	17.69201208967833
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	54.78054497062629
2	33.71020987737512
3	33.95017253223412
4	54.61176004019095
5	54.094442836901315
6	54.67208102098672
7	33.900350552813585
8	33.694070076616285
9	54.04986527636716
10	33.98495237996506
11	54.26407336250897
12	54.335391361202134
13	34.04949125579541
14	54.06232585302353
15	34.047133300017045
16	54.095449033548114
17	54.158036497699825
18	34.24091254514799
19	54.194179254945205
20	34.28984477922492
21	54.313495158371026
22	34.290032468406956
23	34.256774728481844
24	54.29734899707095
25	34.47441114882147
26	54.23617371480272
27	54.40900389201465
28	34.57616563782513
29	54.19608968913758
30	34.75453631698672
31	54.304605535343796
32	34.69249486191191
33	54.44868333344007
34	54.53343445644767
35	54.45157420984551
36	54.40670416637951
37	34.725828949858936
38	54.25028443168243
39	54.39925025716504
40	54.45188934822549
41	54.305297134443755
42	35.63393282848461
43	34.672146727667666
44	54.00724926245215
45	34.95995553935668
46	54.102648475797544
47	54.242689113624564
48	34.911915811283656
49	34.84575945497601
50	34.69172782659626
51	54.25768744745331
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	109.5
2	78.0
3	152.5
4	227.0
5	411.5
6	596.0
7	1158.5
8	1721.0
9	3194.0
10	4667.0
11	7652.0
12	10637.0
13	16105.5
14	21574.0
15	30535.5
16	39497.0
17	52751.0
18	66005.0
19	84605.0
20	103205.0
21	125767.5
22	148330.0
23	173855.0
24	199380.0
25	224867.5
26	276338.0
27	302321.0
28	325262.0
29	348203.0
30	369097.0
31	389991.0
32	402286.5
33	414582.0
34	424696.0
35	434810.0
36	438077.0
37	441344.0
38	441548.0
39	441752.0
40	439764.5
41	437777.0
42	431842.5
43	425908.0
44	414771.0
45	403634.0
46	384645.5
47	365657.0
48	347158.0
49	328659.0
50	314128.0
51	299597.0
52	285875.5
53	272154.0
54	312199.0
55	352244.0
56	1136442.5
57	1920641.0
58	1120530.0
59	320419.0
60	266648.0
61	212877.0
62	199248.0
63	185619.0
64	175387.0
65	165155.0
66	155955.5
67	146756.0
68	134324.0
69	121892.0
70	108221.5
71	94551.0
72	81064.0
73	67577.0
74	56082.0
75	35581.5
76	26576.0
77	20484.5
78	14393.0
79	10850.5
80	7308.0
81	5285.0
82	3262.0
83	2314.0
84	1366.0
85	986.0
86	606.0
87	419.5
88	233.0
89	169.0
90	105.0
91	68.5
92	32.0
93	25.0
94	18.0
95	12.5
96	7.0
97	6.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0463053729579671
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.081801246484537E-4
20	1.3212683240859796E-4
21	0.0072161577700080425
22	0.0023986101883407013
23	2.032720498593815E-5
24	0.0
25	0.0
26	0.06560605409211538
27	0.07221239571254527
28	0.08367693932461438
29	0.6556946512314068
30	0.09625947921091009
31	0.015458839391805961
32	0.07676568962939541
33	0.023721848218589817
34	0.002022556896100846
35	0.01581456547905988
36	0.011068163114843321
37	0.01732894225051227
38	0.0011484870817055053
39	0.010072130070532351
40	0.026659129339057883
41	0.009919676033137816
42	0.02460608163547813
43	0.0095639499458839
44	0.02764499878087588
45	0.0022055017409742888
46	0.018172521257428704
47	0.012216650196548827
48	0.009584277150869836
49	0.022898596416659322
50	0.029820009714371265
51	0.025825713934634415
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9839031.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.331133474968045
#Duplication Level	Relative count
1	100.0
2	12.59327967445618
3	2.0651686065647348
4	0.8719068848927322
5	0.6319380030519733
6	0.4978905478591305
7	0.41171718380658867
8	0.3189611322222555
9	0.26450435355016305
10++	2.029861464348763
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG	1611511	16.378757217047085	TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTATG	29876	0.303647788079944	TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTCTG	13543	0.13764566856228017	TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGGTCTCGTATG	10653	0.10827285735759956	TruSeq Adapter, Index 7 (97% over 36bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGAAG	2393805	6.6485233	260.89066	5
GATCG	2430760	6.303123	242.97726	1
ATCGG	2350565	6.095172	243.46045	2
CGGAA	2421720	6.0948005	236.54973	4
GAGCA	2403865	6.049864	235.4513	9
GTCTG	2261170	6.041226	247.89514	17
TCGGA	2323215	6.0242515	243.66014	3
CCGTC	2221030	5.989791	243.96378	34
TCCCG	2170870	5.8545175	240.33327	37
GAAGA	2643145	5.7957826	206.35019	6
TCCAG	2464320	5.790418	216.35825	25
CGTCC	2143740	5.7813516	242.81973	35
CCCGT	2134585	5.756661	244.37967	33
AGAGC	2279870	5.737803	235.2563	8
TCTCG	2324730	5.628122	212.65625	43
ACCCG	2143745	5.611154	238.47253	32
GTCCC	2076320	5.5995297	241.67328	36
CGTCT	2277155	5.5129437	224.44977	16
CCCGA	2073610	5.427579	223.63826	38
CTGAA	2611260	5.3458714	190.79582	19
CGATC	2267930	5.32896	199.65182	40
ACGTC	2246225	5.27796	218.22797	15
GTCAC	2241605	5.267104	215.63582	29
AAGAG	2399375	5.2612534	205.55893	7
CCAGT	2234555	5.250539	215.09274	26
CTCGT	2163805	5.2385263	211.33676	44
CACGT	2229400	5.2384257	218.26448	14
AGCAC	2292025	5.2270184	212.89005	10
GCACA	2280890	5.2016244	212.73262	11
TCTGA	2444760	5.1568294	196.27937	18
CCGAT	2185020	5.134146	199.61938	39
CACCC	2157460	5.117065	216.29433	31
CAGTC	2173265	5.1065254	214.9345	27
ACACG	2212515	5.0456934	212.13402	13
CTCCA	2368130	5.0421705	195.88911	24
TCACC	2362490	5.030161	194.82397	30
TGAAC	2408355	4.9304767	190.24455	20
TTTTT	3084325	4.894986	5.1862173	2
GAACT	2386835	4.8864193	189.99324	21
CACAC	2294715	4.7420106	192.39705	12
GTATG	2022625	4.708286	198.65768	47
ACTCC	2199975	4.6841373	196.53253	23
GATCT	2204055	4.649101	179.55598	41
AGTCA	2243085	4.592129	187.5918	28
TCGTA	2080645	4.3887877	181.2485	45
AACTC	2352415	4.363967	171.92998	22
CGTAT	2052020	4.3284073	180.9538	46
ATCTC	2244835	4.290719	162.88158	42
AAAAA	3130130	4.2782173	4.5434365	13
ATTTT	2252465	3.4695342	3.6419764	12
AAAAT	2266705	3.1920793	3.372016	15
TTTTC	1719485	3.0398767	3.4088728	2
CTCTC	480555	1.0542247	6.4040594	42
CCCGC	291980	0.87715554	8.242802	38
CGCTC	314255	0.8474995	7.191309	40
CCGCT	300590	0.8106469	7.0391903	39
GCTCT	334465	0.8097326	6.530021	41
>>END_MODULE