FastQCFastQC Report
Wed 23 Jul 2014
LW196_ATGTCA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW196_ATGTCA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13045884
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG 449918 3.448735248604081 TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACAC 1309485 2.6400266 50.245804 12
GGAAG 1359570 2.6285522 49.61455 5
CTCCA 1253335 2.598445 50.986355 24
TCCAG 1238905 2.532912 50.439507 25
CCCAG 855780 2.312535 5.759784 1
CAGAA 1554300 2.273504 35.923508 38
GAGCA 1130280 2.2159772 49.113754 9
GCACA 1114470 2.215705 49.57941 11
CCAGT 1067290 2.1820495 49.926247 26
GAAGA 1511395 2.1800907 37.096004 6
AGAGC 1109345 2.1749332 49.143208 8
GTCTG 1046030 2.1687057 51.38361 17
ACTCC 1022630 2.1201417 50.57619 23
AGCAC 1058045 2.103525 49.47067 10
CATGT 1358920 2.101996 38.20812 33
GTCAG 1029775 2.076157 48.764736 36
AAGAG 1433540 2.06779 36.912148 7
ACATG 1366995 2.0562048 37.289566 32
CTGAA 1365305 2.0536628 37.678005 19
TCACA 1343465 2.049227 37.797047 30
CAGTC 981155 2.0059485 49.891933 27
TCTGA 1285160 1.9879031 38.702023 18
CACAT 1301875 1.9857885 37.618828 31
GTCAC 955930 1.9543762 49.974567 29
TCAGA 1291170 1.9421505 36.745304 37
TGTCA 1218525 1.8848312 37.732063 35
ATCTC 1198230 1.8795003 38.18554 42
TGAAC 1177100 1.7705686 37.38006 20
GATCG 873325 1.760734 50.234062 1
GAATC 1145815 1.7235105 36.36834 40
ATGTC 1090855 1.6873494 37.599483 34
AACTC 1106025 1.6870526 37.519363 22
AGAAT 1513850 1.6753271 27.359688 39
TCTCG 793965 1.6692526 50.467606 43
AGTCA 1097455 1.6507683 37.016956 28
GAACT 1082465 1.6282208 37.21267 21
CGGAA 814755 1.5973731 48.91448 4
CGTCT 750330 1.5775133 51.313244 16
ATCGG 781185 1.5749679 50.156097 2
CACGT 759765 1.5533218 50.055565 14
ACACG 754980 1.5009941 48.77621 13
ACGTC 730350 1.4931835 49.95744 15
CTCGT 710075 1.4928801 50.11063 44
TCGGA 737860 1.4876194 50.115402 3
AATCT 1271720 1.4676112 28.084898 41
GTATG 948805 1.447274 36.616795 47
CGTAT 700660 1.0837904 36.834682 46
TCGTA 688520 1.0650122 36.88063 45
Produced by FastQC (version 0.10.1)