##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW196_ATGTCA_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	13045884	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76262666447134	34.0	31.0	34.0	31.0	34.0
2	32.94027855835603	34.0	31.0	34.0	31.0	34.0
3	32.99044579884353	34.0	31.0	34.0	31.0	34.0
4	36.36380654618729	37.0	37.0	37.0	35.0	37.0
5	36.304162063682305	37.0	37.0	37.0	35.0	37.0
6	36.29753629573895	37.0	37.0	37.0	35.0	37.0
7	36.26490056174039	37.0	37.0	37.0	35.0	37.0
8	36.26196538310474	37.0	37.0	37.0	35.0	37.0
9	38.05022434662151	39.0	38.0	39.0	35.0	39.0
10	38.00674802872692	39.0	38.0	39.0	35.0	39.0
11	37.998416895321164	39.0	38.0	39.0	35.0	39.0
12	37.96670145158427	39.0	38.0	39.0	35.0	39.0
13	37.96739661336863	39.0	38.0	39.0	35.0	39.0
14	39.50051870766289	41.0	39.0	41.0	37.0	41.0
15	39.448415530906146	41.0	39.0	41.0	37.0	41.0
16	39.43623797360148	41.0	39.0	41.0	37.0	41.0
17	39.40684318517626	41.0	39.0	41.0	36.0	41.0
18	39.31504227693578	41.0	39.0	41.0	36.0	41.0
19	39.38812456097264	41.0	39.0	41.0	36.0	41.0
20	39.3721957055574	41.0	39.0	41.0	36.0	41.0
21	39.334555711211294	41.0	39.0	41.0	36.0	41.0
22	39.23427120768512	40.0	39.0	41.0	36.0	41.0
23	39.17387514713453	40.0	39.0	41.0	36.0	41.0
24	39.145818558558396	40.0	39.0	41.0	36.0	41.0
25	39.12778137533647	40.0	39.0	41.0	36.0	41.0
26	39.01966643272315	40.0	39.0	41.0	36.0	41.0
27	38.92938761374852	40.0	39.0	41.0	36.0	41.0
28	38.77213740364394	40.0	38.0	41.0	35.0	41.0
29	38.50680176214966	40.0	38.0	41.0	35.0	41.0
30	38.465470028707905	40.0	38.0	41.0	34.0	41.0
31	38.712174429881486	40.0	38.0	41.0	35.0	41.0
32	38.679659346963376	40.0	38.0	41.0	35.0	41.0
33	38.72892385061832	40.0	38.0	41.0	35.0	41.0
34	38.87101901258665	40.0	39.0	41.0	35.0	41.0
35	38.931465587153774	40.0	39.0	41.0	35.0	41.0
36	38.91623097369254	40.0	39.0	41.0	35.0	41.0
37	38.81816579083487	40.0	38.0	41.0	35.0	41.0
38	38.782816787271756	40.0	38.0	41.0	35.0	41.0
39	38.75279421463505	40.0	38.0	41.0	35.0	41.0
40	38.689297252681385	40.0	38.0	41.0	35.0	41.0
41	38.60995851258527	40.0	38.0	41.0	35.0	41.0
42	38.56072466994188	40.0	38.0	41.0	35.0	41.0
43	38.54796141066409	40.0	38.0	41.0	34.0	41.0
44	38.50291747190148	40.0	38.0	41.0	34.0	41.0
45	38.43992097430883	40.0	38.0	41.0	34.0	41.0
46	38.433676859306736	40.0	38.0	41.0	34.0	41.0
47	38.29942348099983	40.0	38.0	41.0	34.0	41.0
48	38.11041490174219	40.0	38.0	41.0	33.0	41.0
49	38.08829666123047	40.0	38.0	41.0	33.0	41.0
50	38.02880456395289	40.0	37.0	41.0	33.0	41.0
51	37.04958008211632	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	5.0
12	9.0
13	6.0
14	22.0
15	51.0
16	152.0
17	323.0
18	788.0
19	1514.0
20	2688.0
21	4524.0
22	7183.0
23	11251.0
24	16172.0
25	23211.0
26	32306.0
27	44051.0
28	60125.0
29	80745.0
30	107566.0
31	138589.0
32	179106.0
33	232379.0
34	315423.0
35	445501.0
36	680615.0
37	1143333.0
38	2428351.0
39	7085383.0
40	4507.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	26.65722877296365	20.067407756807548	27.017503149266286	26.257860320962518
2	21.72334967871859	32.32135898188272	19.590868660184316	26.36442267921438
3	15.709422220832256	28.71667416328399	33.88920980747645	21.68469380840731
4	20.299958209041257	28.200488368591962	28.433036810690638	23.066516611676143
5	25.16583774621942	30.402109968170805	27.157784018315663	17.27426826729411
6	25.362282847218324	29.684305026780862	26.57662754014983	18.376784585850984
7	19.678390517652925	32.76021004019352	28.96162498455452	18.599774457599043
8	19.64087677002187	32.13829741242525	28.97422666030144	19.24659915725144
9	24.65376819232794	27.12487708766995	28.213879565386296	20.00747515461582
10	21.513130118280984	31.232287516890384	26.771439942283713	20.48314242254492
11	25.211622301716	27.249100175963548	27.052816045275275	20.48646147704517
12	20.700912257076638	27.39897120041846	27.65569585012407	24.24442069238083
13	20.506000206655216	31.636874894794403	27.599869813344963	20.257255085205415
14	20.315564663920053	27.947757315640704	27.6966972878189	24.039980732620343
15	20.37832775456228	32.019432335899964	27.70982020076217	19.89241970877558
16	20.73495364514969	27.975850467473112	27.38769561342106	23.901500273956138
17	24.814692511446523	28.079722309350597	27.252718175326407	19.85286700387647
18	21.07821133470143	28.13783259149016	31.006331192274892	19.77762488153352
19	21.093784134857803	27.77868569845563	27.18356157999252	23.94396858669405
20	21.56107627509182	27.82953612035796	30.65101605993124	19.958371544618977
21	25.218281189532437	27.521115924154397	26.81855760390991	20.442045282403253
22	20.579658998777926	31.490845728501043	27.46676976948349	20.462725503237536
23	20.158442310233635	31.150882531225943	27.950670111737924	20.7400050468025
24	20.305514126557874	27.355880809278304	27.81186849629998	24.526736567863843
25	20.309271491299477	27.56466330683302	31.89617506947019	20.229890132397312
26	20.53445617834049	27.78890249941039	27.62253036933922	24.054110952909895
27	20.7294815799359	28.097691395963558	27.1450886288004	24.027738395300144
28	21.250336918328543	32.136183462851186	26.665880779722645	19.94759883909763
29	25.36156885242637	27.688421753367905	26.805497640487374	20.14451175371835
30	21.196402935845672	27.592723065866476	30.679243408981023	20.531630589306825
31	20.724564002527345	27.726241741658846	26.912476919585078	24.636717336228735
32	20.184211374551616	31.597181611249	27.46940554744615	20.749201466753238
33	20.17175117507304	27.812919018665472	27.54698567219698	24.468344134064505
34	20.257435206142276	31.508555619576743	27.825320071031832	20.408689103249145
35	20.56463768080387	27.814141885594967	31.456891193076313	20.164329240524843
36	24.645275383708018	28.08144151471851	27.38719706595978	19.886086035613697
37	20.48074475853757	28.423058822915948	31.279680673167192	19.816515745379288
38	20.59727606861941	28.26055426436635	27.383232979338466	23.75893668767577
39	20.944604567776253	31.938500885288875	27.09218542345711	20.02470912347777
40	25.11455923122065	27.74621283275004	26.95768895123502	20.181538984794287
41	21.105089698938762	31.393256307980792	26.914871678502077	20.586782314578368
42	20.4213406351481	31.542887607486776	27.419455740235634	20.6163160171295
43	20.142062493727543	27.50197364390902	31.800411756899205	20.55555210546423
44	20.490212606494335	27.40766108323445	27.751250424628036	24.350875885643177
45	20.336995847201806	27.924432014912163	31.54124752008239	20.197324617803638
46	20.671560684140218	27.9970234772998	27.560189790889005	23.771226047670975
47	24.383735294149215	28.31387118599995	27.37949493117402	19.92289858867682
48	20.57867372173202	28.580935374812107	31.312907401960526	19.527483501495347
49	20.871274701961205	31.972188131283414	27.368596647792355	19.787940518963023
50	21.316852413440557	27.97599174425846	30.728971214610105	19.978184627690883
51	25.012319387204396	27.85667194092827	26.698174246884506	20.43283442498283
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	52.91508909392616
2	48.08777235793297
3	37.39411602923957
4	43.3664748207174
5	42.440106013513535
6	43.73906743306931
7	38.27816497525197
8	38.88747592727331
9	44.661243346943756
10	41.9962725408259
11	45.69808377876118
12	44.945332949457466
13	40.76325529186063
14	44.3555453965404
15	40.27074746333786
16	44.636453919105826
17	44.667559515323
18	40.85583621623494
19	45.03775272155185
20	41.5194478197108
21	45.66032647193569
22	41.042384502015466
23	40.89844735703613
24	44.83225069442172
25	40.53916162369679
26	44.588567131250386
27	44.75721997523604
28	41.197935757426166
29	45.50608060614472
30	41.7280335251525
31	45.36128133875608
32	40.93341284130485
33	44.64009530913755
34	40.666124309391414
35	40.72896692132871
36	44.53136141932171
37	40.29726050391686
38	44.356212756295186
39	40.96931369125402
40	45.29609821601494
41	41.69187201351713
42	41.0376566522776
43	40.69761459919177
44	44.84108849213751
45	40.53432046500545
46	44.442786731811196
47	44.30663388282603
48	40.10615722322737
49	40.65921522092423
50	41.29503704113144
51	45.44515381218722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	224.0
1	257.0
2	290.0
3	406.5
4	523.0
5	728.5
6	934.0
7	1363.0
8	1792.0
9	2676.0
10	3560.0
11	5555.5
12	7551.0
13	11327.5
14	15104.0
15	22626.0
16	30148.0
17	42171.5
18	54195.0
19	73476.5
20	92758.0
21	120268.5
22	147779.0
23	184706.0
24	221633.0
25	265943.5
26	360358.0
27	410462.0
28	462598.0
29	514734.0
30	567998.5
31	621263.0
32	671392.0
33	721521.0
34	766307.0
35	811093.0
36	841231.0
37	871369.0
38	877209.0
39	883049.0
40	868305.5
41	853562.0
42	822040.0
43	790518.0
44	753394.0
45	716270.0
46	698905.0
47	681540.0
48	858833.5
49	1036127.0
50	780697.0
51	525267.0
52	494401.0
53	463535.0
54	440124.0
55	416713.0
56	395310.0
57	373907.0
58	350858.0
59	327809.0
60	302194.5
61	276580.0
62	251983.0
63	227386.0
64	204116.0
65	180846.0
66	160661.5
67	140477.0
68	123123.5
69	105770.0
70	91227.5
71	76685.0
72	64714.5
73	52744.0
74	43358.5
75	27333.5
76	20694.0
77	16172.0
78	11650.0
79	8972.0
80	6294.0
81	4925.5
82	3557.0
83	2740.5
84	1924.0
85	1445.0
86	966.0
87	701.5
88	437.0
89	322.0
90	207.0
91	161.5
92	116.0
93	85.0
94	54.0
95	39.5
96	25.0
97	18.0
98	11.0
99	7.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04250382726076669
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0015483810832596704
20	0.0
21	0.013030929908620988
22	5.365677021196877E-5
23	0.0
24	0.0010501396455771031
25	0.0
26	0.0908332467159757
27	0.11382134012536062
28	0.12279735125653424
29	0.6982278855154622
30	0.13655648018946054
31	0.034677604062706674
32	0.1206817414596052
33	0.01543781931527216
34	0.012203082596779184
35	0.01680223432923365
36	0.0301704353649013
37	0.039866980267492795
38	0.008140498566444405
39	0.01624267086845169
40	0.025188020988075627
41	0.012984938391296443
42	0.016756242811909104
43	0.021087110693303726
44	0.016212009856901993
45	0.02385426698566383
46	0.018979166149262094
47	0.009550905097730442
48	0.03139687582688915
49	0.011689510653321768
50	0.01434168815237051
51	0.01076201505394345
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.3045884E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	11.331224095319604
#Duplication Level	Relative count
1	100.0
2	5.5952019435165505
3	0.7694893930848987
4	0.41972148713721746
5	0.2705956357641751
6	0.20823391609908465
7	0.14587219643399418
8	0.11984295692160861
9	0.09544054487874713
10++	0.8307665611036398
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG	449918	3.448735248604081	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	1309485	2.6400266	50.245804	12
GGAAG	1359570	2.6285522	49.61455	5
CTCCA	1253335	2.598445	50.986355	24
TCCAG	1238905	2.532912	50.439507	25
CCCAG	855780	2.312535	5.759784	1
CAGAA	1554300	2.273504	35.923508	38
GAGCA	1130280	2.2159772	49.113754	9
GCACA	1114470	2.215705	49.57941	11
CCAGT	1067290	2.1820495	49.926247	26
GAAGA	1511395	2.1800907	37.096004	6
AGAGC	1109345	2.1749332	49.143208	8
GTCTG	1046030	2.1687057	51.38361	17
ACTCC	1022630	2.1201417	50.57619	23
AGCAC	1058045	2.103525	49.47067	10
CATGT	1358920	2.101996	38.20812	33
GTCAG	1029775	2.076157	48.764736	36
AAGAG	1433540	2.06779	36.912148	7
ACATG	1366995	2.0562048	37.289566	32
CTGAA	1365305	2.0536628	37.678005	19
TCACA	1343465	2.049227	37.797047	30
CAGTC	981155	2.0059485	49.891933	27
TCTGA	1285160	1.9879031	38.702023	18
CACAT	1301875	1.9857885	37.618828	31
GTCAC	955930	1.9543762	49.974567	29
TCAGA	1291170	1.9421505	36.745304	37
TGTCA	1218525	1.8848312	37.732063	35
ATCTC	1198230	1.8795003	38.18554	42
TGAAC	1177100	1.7705686	37.38006	20
GATCG	873325	1.760734	50.234062	1
GAATC	1145815	1.7235105	36.36834	40
ATGTC	1090855	1.6873494	37.599483	34
AACTC	1106025	1.6870526	37.519363	22
AGAAT	1513850	1.6753271	27.359688	39
TCTCG	793965	1.6692526	50.467606	43
AGTCA	1097455	1.6507683	37.016956	28
GAACT	1082465	1.6282208	37.21267	21
CGGAA	814755	1.5973731	48.91448	4
CGTCT	750330	1.5775133	51.313244	16
ATCGG	781185	1.5749679	50.156097	2
CACGT	759765	1.5533218	50.055565	14
ACACG	754980	1.5009941	48.77621	13
ACGTC	730350	1.4931835	49.95744	15
CTCGT	710075	1.4928801	50.11063	44
TCGGA	737860	1.4876194	50.115402	3
AATCT	1271720	1.4676112	28.084898	41
GTATG	948805	1.447274	36.616795	47
CGTAT	700660	1.0837904	36.834682	46
TCGTA	688520	1.0650122	36.88063	45
>>END_MODULE