##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW195_AGTTCC_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7034302	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.762460582443005	34.0	31.0	34.0	31.0	34.0
2	32.941499526179	34.0	31.0	34.0	31.0	34.0
3	33.00472214585044	34.0	31.0	34.0	31.0	34.0
4	36.36031762639705	37.0	37.0	37.0	35.0	37.0
5	36.30286046860086	37.0	37.0	37.0	35.0	37.0
6	36.294467596074206	37.0	37.0	37.0	35.0	37.0
7	36.27338135326007	37.0	37.0	37.0	35.0	37.0
8	36.263868255869596	37.0	37.0	37.0	35.0	37.0
9	38.03707759490565	39.0	38.0	39.0	35.0	39.0
10	37.992197946576646	39.0	38.0	39.0	35.0	39.0
11	37.99805353821886	39.0	38.0	39.0	35.0	39.0
12	37.981245047483036	39.0	38.0	39.0	35.0	39.0
13	37.97890153138151	39.0	38.0	39.0	35.0	39.0
14	39.4824073518595	41.0	39.0	41.0	37.0	41.0
15	39.436212150118095	41.0	39.0	41.0	37.0	41.0
16	39.41429341532394	41.0	39.0	41.0	37.0	41.0
17	39.376604103719174	41.0	39.0	41.0	36.0	41.0
18	39.31622114034911	41.0	39.0	41.0	36.0	41.0
19	39.35730851476095	41.0	39.0	41.0	36.0	41.0
20	39.32245573192621	40.0	39.0	41.0	36.0	41.0
21	39.28493274812483	40.0	39.0	41.0	36.0	41.0
22	39.194752087698255	40.0	39.0	41.0	36.0	41.0
23	39.13513380574221	40.0	39.0	41.0	36.0	41.0
24	39.04623870285922	40.0	39.0	41.0	36.0	41.0
25	39.01433390263881	40.0	39.0	41.0	36.0	41.0
26	38.87440928751708	40.0	38.0	41.0	35.0	41.0
27	38.75943697043431	40.0	38.0	41.0	35.0	41.0
28	38.612230751537254	40.0	38.0	41.0	35.0	41.0
29	38.33283387605479	40.0	38.0	41.0	34.0	41.0
30	38.28435543427052	40.0	38.0	41.0	34.0	41.0
31	38.50699927299112	40.0	38.0	41.0	34.0	41.0
32	38.45572055336834	40.0	38.0	41.0	34.0	41.0
33	38.456856984530944	40.0	38.0	41.0	34.0	41.0
34	38.59776236505058	40.0	38.0	41.0	35.0	41.0
35	38.64222775763679	40.0	38.0	41.0	35.0	41.0
36	38.553568641209885	40.0	38.0	41.0	35.0	41.0
37	38.47599335371157	40.0	38.0	41.0	34.0	41.0
38	38.39986895643661	40.0	38.0	41.0	34.0	41.0
39	38.3362067764506	40.0	38.0	41.0	34.0	41.0
40	38.231006999699474	40.0	38.0	41.0	34.0	41.0
41	38.152817863094306	40.0	37.0	41.0	33.0	41.0
42	38.04225891922184	40.0	37.0	41.0	33.0	41.0
43	37.976131249411814	40.0	37.0	41.0	33.0	41.0
44	37.8647459264615	40.0	37.0	41.0	33.0	41.0
45	37.752292835877675	40.0	37.0	41.0	33.0	41.0
46	37.68492140371568	40.0	36.0	41.0	33.0	41.0
47	37.524676364477955	40.0	36.0	41.0	33.0	41.0
48	37.35613839155612	39.0	35.0	41.0	32.0	41.0
49	37.262795939099576	39.0	35.0	41.0	32.0	41.0
50	37.14487592372349	39.0	35.0	41.0	32.0	41.0
51	36.1252756847801	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	8.0
12	11.0
13	2.0
14	10.0
15	28.0
16	72.0
17	190.0
18	454.0
19	981.0
20	1853.0
21	3070.0
22	4905.0
23	7514.0
24	11048.0
25	15510.0
26	21218.0
27	28839.0
28	38584.0
29	50333.0
30	65173.0
31	82696.0
32	106063.0
33	138437.0
34	188393.0
35	276387.0
36	453520.0
37	781012.0
38	1458958.0
39	3297181.0
40	1847.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.463128276778562	25.94834579571106	27.93529101889609	22.653234908614287
2	20.41874232866317	29.988803437782458	28.332647645779215	21.259806587775163
3	21.085418283150197	26.977573610004235	31.04532617450886	20.891681932336713
4	20.82685673717165	26.75557574866703	27.951031957399614	24.46653555676171
5	23.802304763144942	27.344248228182412	28.006744663507483	20.846702345165163
6	24.226668118599402	27.7060183085685	26.97410489342084	21.093208679411262
7	21.290655419684853	30.69126119407441	27.46480034550692	20.55328304073382
8	20.731097413787467	30.95310664796592	27.545604382638107	20.770191555608502
9	23.69151907324991	27.59227567994664	27.77678581329036	20.93941943351309
10	20.821738958605984	30.744713547982442	27.53379084378237	20.899756649629204
11	23.707824884402175	27.68893061457981	27.645514793081105	20.957729707936906
12	20.812711765858218	27.573823245007112	27.564497515176345	24.048967473958328
13	20.691619438574005	30.69998984973918	27.650390898770056	20.95799981291676
14	20.641777961765076	27.771099392661846	27.704596703411372	23.88252594216171
15	20.69709261842895	30.657270614767462	27.683912348375152	20.96172441842844
16	20.73321560547159	27.738701011130885	27.642927471695128	23.885155911702398
17	23.702479649011373	27.692157658286494	27.68556141035742	20.919801282344714
18	20.890359839540583	27.559720921848395	30.580660312849805	20.96925892576122
19	20.852976675352814	27.53437140594724	27.61955534325914	23.993096575440813
20	20.844331227793635	27.496577129696327	30.59891807302531	21.060173569484732
21	23.876294305900004	27.445206822358966	27.573380908217544	21.10511796352349
22	20.942194540722667	30.438617852719123	27.566619625745332	21.052567980812878
23	20.870613743908066	30.56348732255169	27.566729435273036	20.999169498267207
24	20.943508829393174	27.483739518400874	27.48436503089463	24.088386621311326
25	20.913944269097346	27.506908290261066	30.47075601815219	21.108391422489394
26	20.89938925265088	27.536121146047343	27.508588212176328	24.055901389125445
27	20.98264116550446	27.50728639536517	27.403763293873396	24.106309145256972
28	20.996802286935605	30.520908682276332	27.40636234732009	21.07592668346797
29	23.93615315592341	27.571977225655093	27.44763223429883	21.044237384122667
30	21.085225168976557	27.42431767720919	30.320233014761577	21.170224139052678
31	21.022712970988575	27.492798872564045	27.400163682995384	24.084324473452
32	20.96340057624582	30.41062389719294	27.40892487867254	21.217050647888694
33	21.013825422072987	27.486452474071914	27.39306685730527	24.10665524654983
34	21.04121987020794	30.440907418370962	27.42454436002823	21.093328351392866
35	23.919562036468427	27.60196539305515	27.463733260041774	21.01473931043465
36	21.049735871680337	27.51093969615147	30.31221577125071	21.127108660917486
37	20.996288284224217	27.561171348609026	30.349615780032735	21.092924587134018
38	20.95915267705082	27.54516132196734	27.492273301971114	24.003412699010724
39	21.060355149390322	27.474974595320194	27.393816116327912	24.070854138961572
40	23.964215382852146	27.565343229076237	27.383345853740316	21.087095534331297
41	21.021885190615336	27.550205930760402	30.335830359915327	21.09207851870893
42	21.069893489811793	30.322871893452845	27.43813920686807	21.1690954098673
43	21.01433613883179	27.512947798163363	30.31917616399369	21.153539899011157
44	20.982270884527786	27.475230807794937	27.51872431924266	24.02377398843462
45	21.034794457787775	27.50199320511506	30.313269136510986	21.14994320058618
46	21.045093429572994	27.590125860977068	27.435639822448405	23.92914088700153
47	23.876107925941028	27.60105083599696	27.458919552015615	21.063921686046395
48	21.098790491735485	27.529274980468177	30.288601692179622	21.083332835616712
49	21.134819594488537	30.40571050949401	27.408989679089522	21.05048021692793
50	21.054724633691848	27.519554870937995	30.269876697748394	21.155843797621763
51	23.9766303033474	27.4302617752365	27.35016001159016	21.242947909825943
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.11636318539285
2	41.67854891643834
3	41.977100215486914
4	45.29339229393335
5	44.64900710831011
6	45.31987679801066
7	41.84393846041867
8	41.501288969395965
9	44.630938506763
10	41.72149560823519
11	44.665554592339085
12	44.861679239816546
13	41.64961925149077
14	44.524303903926786
15	41.658817036857386
16	44.61837151717399
17	44.62228093135609
18	41.8596187653018
19	44.84607325079362
20	41.90450479727836
21	44.981412269423494
22	41.99476252153554
23	41.869783242175274
24	45.031895450704496
25	42.02233569158674
26	44.95529064177632
27	45.08895031076143
28	42.07272897040358
29	44.98039054004607
30	42.255449308029235
31	45.10703744444057
32	42.180451224134515
33	45.12048066862281
34	42.13454822160081
35	44.93430134690308
36	42.17684453259782
37	42.08921287135824
38	44.96256537606155
39	45.1312092883519
40	45.05131091718344
41	42.11396370932427
42	42.23898889967909
43	42.16787603784295
44	45.006044872962406
45	42.184737658373955
46	44.974234316574524
47	44.94002961198743
48	42.1821233273522
49	42.18529981141647
50	42.21056843131361
51	45.21957821317334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	88.5
2	67.0
3	138.5
4	210.0
5	445.0
6	680.0
7	1250.5
8	1821.0
9	3262.5
10	4704.0
11	7805.0
12	10906.0
13	16347.0
14	21788.0
15	30231.0
16	38674.0
17	51110.0
18	63546.0
19	80316.5
20	97087.0
21	117446.5
22	137806.0
23	161036.0
24	184266.0
25	205868.5
26	248470.0
27	269469.0
28	284600.5
29	299732.0
30	313681.0
31	327630.0
32	333177.0
33	338724.0
34	346741.0
35	354758.0
36	357089.5
37	359421.0
38	357449.5
39	355478.0
40	352813.5
41	350149.0
42	344526.0
43	338903.0
44	331413.5
45	323924.0
46	311900.5
47	299877.0
48	293146.0
49	286415.0
50	368208.5
51	450002.0
52	344619.0
53	239236.0
54	227851.0
55	216466.0
56	210227.0
57	203988.0
58	199467.5
59	194947.0
60	191917.0
61	188887.0
62	183329.0
63	177771.0
64	170412.5
65	163054.0
66	153038.0
67	143022.0
68	130426.0
69	117830.0
70	104051.5
71	90273.0
72	77077.5
73	63882.0
74	52758.0
75	33097.0
76	24560.0
77	18876.5
78	13193.0
79	9928.5
80	6664.0
81	4820.0
82	2976.0
83	2163.0
84	1350.0
85	951.5
86	553.0
87	392.0
88	231.0
89	167.5
90	104.0
91	70.5
92	37.0
93	28.0
94	19.0
95	12.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04273345102328561
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0015921977759840277
20	1.4216051571285962E-5
21	0.012780230362586083
22	1.4216051571285962E-5
23	0.0
24	9.951236099900174E-4
25	0.0
26	0.0903430077355223
27	0.11161022088616611
28	0.12305414240105131
29	0.6951364897327411
30	0.1352088664945008
31	0.03436019664779818
32	0.12056633337607625
33	0.015623440676843273
34	0.012766014311014795
35	0.017130342143399587
36	0.03005273302169853
37	0.039563271522888833
38	0.008145797550346858
39	0.016334243255407572
40	0.025347219951602874
41	0.012367964867018789
42	0.01666121244154715
43	0.020556410572079502
44	0.015196959129704694
45	0.023584429556763414
46	0.0185377312489569
47	0.00943945824333388
48	0.031104720837973693
49	0.011571865979026773
50	0.014443508396426538
51	0.010647822626893188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7034302.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	20.421395402608916
#Duplication Level	Relative count
1	100.0
2	14.958656674858657
3	2.3686153920654713
4	0.7947845667727994
5	0.5024152249972761
6	0.34079490563068243
7	0.2657352816551858
8	0.22215227418554254
9	0.18159586445684678
10++	1.4291594532753842
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG	186089	2.645450820849034	TruSeq Adapter, Index 8 (97% over 37bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2799800	4.726517	5.041993	13
AAAAA	2802985	4.7068663	4.983001	13
CGGCG	549515	3.4386787	3.659612	33
ATTTT	2015070	3.398159	3.58064	13
AAAAT	2015155	3.3875067	3.5519023	14
TTTTC	1452760	3.1628163	3.4568536	1
GAAAA	1438015	3.1576004	3.28346	4
CGCCG	500620	3.0928876	3.4287088	45
GATCG	612245	2.2718976	38.378757	1
TCCAG	592400	2.1703131	37.09132	25
CTGAA	759050	2.1540277	29.455929	19
CGGAA	576360	2.1364696	38.38818	4
GAAGA	741635	2.1294458	30.152433	6
GGAAG	558915	2.09848	38.76171	5
TTCCG	569005	2.0868156	36.1013	36
GAGCA	555310	2.058441	38.07405	9
TCTCG	540060	1.9806603	36.27988	43
ATCGG	524420	1.9459996	38.178047	2
CTCCA	527360	1.9074728	36.413197	24
TCGGA	478135	1.774247	38.06394	3
AGCAC	473095	1.7313908	37.243835	10
TCTGA	606490	1.7229201	29.06233	18
GTTCC	462100	1.6947435	35.80441	35
CAGTT	587940	1.6702231	28.276527	33
CCAGT	455155	1.6675031	36.47902	26
GCACA	455290	1.6662297	37.14246	11
AGAGC	448935	1.6641265	37.615475	8
CACAG	449010	1.6432467	36.078094	31
CGTAT	572135	1.6253241	27.501114	46
TGAAC	572440	1.6244669	28.97119	20
CACAC	445845	1.6109219	36.547318	12
GTCTG	430285	1.5983813	37.3258	17
GTCAC	434915	1.5933522	36.277233	29
CGTCT	427650	1.5683987	36.80368	16
ACGTC	426275	1.5616986	36.88507	15
CTCGT	422945	1.5511433	35.742393	44
AAGAG	535160	1.5365971	29.421118	7
ATCTC	539070	1.5119258	27.589485	42
GAACT	528035	1.4984547	28.773312	21
TCACA	531755	1.4898285	27.9567	30
AGTTC	520370	1.4782699	27.98177	34
CACGT	403330	1.4776374	36.85163	14
TCCGT	402340	1.4755749	35.551605	37
ACACG	393980	1.4418529	36.8581	13
AACTC	511085	1.431917	28.376501	22
CAGTC	385590	1.4126453	36.214874	27
ACTCC	387875	1.4029527	36.078526	23
ACAGT	471640	1.3384173	27.956388	32
CCGTA	348330	1.2761396	35.152203	38
AGTCA	445050	1.2629603	28.161802	28
TCGTA	385425	1.0949174	27.511765	45
GTATC	379975	1.079435	27.452328	40
GTATG	364180	1.0478854	27.728018	47
TATCT	461735	1.0041816	21.204065	41
>>END_MODULE