##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW194_AGTCAA_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6608247	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73878337174745	34.0	31.0	34.0	31.0	34.0
2	32.9030894274987	34.0	31.0	34.0	31.0	34.0
3	32.972270558288756	34.0	31.0	34.0	31.0	34.0
4	36.34095350854773	37.0	37.0	37.0	35.0	37.0
5	36.2787327713386	37.0	37.0	37.0	35.0	37.0
6	36.263525863969676	37.0	37.0	37.0	35.0	37.0
7	36.20906149543139	37.0	37.0	37.0	35.0	37.0
8	36.23034550615314	37.0	37.0	37.0	35.0	37.0
9	37.988344261344956	39.0	38.0	39.0	35.0	39.0
10	37.90917455113285	39.0	38.0	39.0	35.0	39.0
11	37.93471445604258	39.0	38.0	39.0	35.0	39.0
12	37.93322510493328	39.0	38.0	39.0	35.0	39.0
13	37.924893243245904	39.0	38.0	39.0	35.0	39.0
14	39.47883334263989	41.0	39.0	41.0	37.0	41.0
15	39.384553876391124	41.0	39.0	41.0	36.0	41.0
16	39.400555548241464	41.0	39.0	41.0	36.0	41.0
17	39.35266554049811	41.0	39.0	41.0	36.0	41.0
18	39.254514850912805	40.0	39.0	41.0	36.0	41.0
19	39.32299957916222	41.0	39.0	41.0	36.0	41.0
20	39.311376829588845	40.0	39.0	41.0	36.0	41.0
21	39.24988472737172	40.0	39.0	41.0	36.0	41.0
22	39.13475283233208	40.0	39.0	41.0	36.0	41.0
23	39.039476278656046	40.0	39.0	41.0	36.0	41.0
24	39.02421822307792	40.0	39.0	41.0	36.0	41.0
25	38.96996540837532	40.0	38.0	41.0	35.0	41.0
26	38.87722916531419	40.0	38.0	41.0	35.0	41.0
27	38.78117373639333	40.0	38.0	41.0	35.0	41.0
28	38.41142711523949	40.0	38.0	41.0	34.0	41.0
29	38.24708685979807	40.0	38.0	41.0	34.0	41.0
30	38.08515299140604	40.0	38.0	41.0	34.0	41.0
31	38.37264330464645	40.0	38.0	41.0	34.0	41.0
32	38.263083235236216	40.0	38.0	41.0	34.0	41.0
33	38.37481740618957	40.0	38.0	41.0	34.0	41.0
34	38.45425708209757	40.0	38.0	41.0	34.0	41.0
35	38.52767761253476	40.0	38.0	41.0	34.0	41.0
36	38.19744162105321	40.0	38.0	41.0	33.0	41.0
37	38.26832880187438	40.0	38.0	41.0	34.0	41.0
38	38.26470514797646	40.0	38.0	41.0	34.0	41.0
39	38.13384396799938	40.0	38.0	41.0	33.0	41.0
40	38.07247262398031	40.0	37.0	41.0	33.0	41.0
41	38.084517421942614	40.0	37.0	41.0	33.0	41.0
42	38.01572746902469	40.0	37.0	41.0	33.0	41.0
43	37.940557457976375	40.0	37.0	41.0	33.0	41.0
44	37.85213355372461	40.0	37.0	41.0	33.0	41.0
45	37.65959731832058	40.0	36.0	41.0	33.0	41.0
46	37.38626961129025	39.0	36.0	41.0	33.0	41.0
47	37.29634742769149	39.0	35.0	41.0	32.0	41.0
48	37.27930917231151	39.0	35.0	41.0	32.0	41.0
49	37.16802126191712	39.0	35.0	41.0	32.0	41.0
50	37.07825766803208	39.0	35.0	41.0	32.0	41.0
51	36.14255596075631	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	5.0
12	12.0
13	16.0
14	6.0
15	29.0
16	77.0
17	175.0
18	459.0
19	940.0
20	1804.0
21	2998.0
22	4806.0
23	7452.0
24	10764.0
25	15574.0
26	21387.0
27	29427.0
28	38860.0
29	50997.0
30	65466.0
31	84011.0
32	106411.0
33	137995.0
34	186296.0
35	273082.0
36	446696.0
37	757321.0
38	1363658.0
39	2999866.0
40	1647.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.26713714927266	23.557229400843188	26.021540505548778	22.15409294433538
2	19.403330414253585	34.44104011245342	25.745829415879882	20.409800057413108
3	20.069225620652496	24.237819803043077	35.693338944503736	19.999615631800687
4	19.62847333037037	23.89160090414296	25.52468150781894	30.95524425766773
5	30.223900529141844	24.576729653113752	25.537211305812267	19.662158511932134
6	30.392008652218962	25.01864715407883	24.367687830070516	20.221656363631688
7	20.495692730613733	35.18143313953004	24.949309552139926	19.373564577716298
8	19.787986133084917	35.38257574209923	25.0501078425186	19.779330282297256
9	29.806346524274897	24.851462119984316	25.387519564568333	19.954671791172455
10	19.965446206838212	35.07016308561106	24.99115120848237	19.97323949906836
11	29.86934356418578	24.95680019224463	25.030813769521632	20.14304247404796
12	19.966036378482826	24.9138084578255	24.92889566627882	30.191259497412855
13	19.836001892786392	35.0176832070593	24.984417198691272	20.161897701463037
14	19.790256024025737	25.15956198368493	24.943498631331426	30.10668336095791
15	19.802543700318708	35.12430755085275	24.964396760593242	20.10875198823531
16	19.899135126153727	25.168535619204306	24.97928724516502	29.953042009476942
17	29.83066462255421	25.068766346052136	25.06953810897202	20.031030922421635
18	20.00743918924338	24.939004247268603	34.95781861664675	20.095737946841272
19	19.881183048563685	24.892935206656993	25.167913914760987	30.057967830018335
20	20.001661560420118	24.853085069774945	35.00007414978196	20.145179220022982
21	29.91743689794112	24.88654062373107	25.0573029063792	20.138719571948613
22	20.00445806580777	34.90545979894517	24.960371487324853	20.12971064792221
23	19.94412436460078	34.904188660018306	24.92387164099647	20.227815334384445
24	20.068581666964278	24.87728845158576	24.965361113044743	30.088768768405217
25	19.9495418376462	24.89011079640334	34.80146852864307	20.35887883730738
26	19.971443271940053	24.918615445503285	24.958662114109682	30.151279168446983
27	20.039059114975174	25.032556837360037	24.867091142782865	30.06129290488192
28	20.142386357042223	34.7937058375861	24.88888612803439	20.175021677337284
29	29.873458070691544	24.975076044158715	25.053485750262496	20.097980134887244
30	20.189677175732403	24.767453242895705	34.6172740880194	20.425595493352493
31	20.06125038771577	24.98709127869986	24.9055139124302	30.046144421154164
32	20.017235405243376	34.676632375139135	24.830210198917726	20.47592202069976
33	20.133311861852818	25.049559466365096	24.828210498403713	29.98891817337837
34	20.239559310634363	34.720254330480095	24.806287311958926	20.233899046926613
35	29.848953325353104	25.093932146066322	24.984384371930886	20.072730156649683
36	20.20332366884166	24.98854883373694	34.51637327562692	20.291754221794484
37	20.03005311068316	25.17972901637525	24.873445248824527	29.916772624117062
38	20.03539195156209	34.95313131600521	24.84464755376176	20.166829178670937
39	20.030388148894364	34.95097228303569	24.84773763576327	20.170901932306684
40	20.0458847103136	34.75106099121509	25.048292923486027	20.15476137498528
41	20.014256828748547	24.984154034289034	34.62405559860519	20.377533538357227
42	20.146619993982277	24.794389349232073	25.129783682762973	29.929206974022684
43	20.098948473809017	24.791228770403183	34.600014257879636	20.509808497908164
44	20.266830124712435	24.890573919360698	24.958938733502844	29.88365722242402
45	29.852597629247256	24.920663607337907	25.039709820881235	20.1870289425336
46	20.176231616620406	25.22832268324427	34.53855743926846	20.056888260866867
47	20.10069514701634	34.70565329639039	25.155913577363993	20.03773797922928
48	20.3830263126425	24.97494012422917	34.52351722887273	20.118516334255602
49	29.861851010608543	24.870525995897996	24.885085200099766	20.38253779339369
50	20.21717129141184	24.81517844075638	24.829389953777017	30.13826031405476
51	20.344029695902133	24.765921691238955	24.8162734802863	30.07377513257261
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	50.42123009360804
2	39.81313047166669
3	40.06884125245318
4	50.5837175880381
5	49.88605904107398
6	50.61366501585065
7	39.86925730833003
8	39.56731641538217
9	49.761018315447345
10	39.93868570590657
11	50.012386038233736
12	50.15729587589568
13	39.997899594249425
14	49.89693938498364
15	39.91129568855401
16	49.852177135630676
17	49.861695544975845
18	40.10317713608465
19	49.93915087858202
20	40.1468407804431
21	50.056156469889736
22	40.13416871372998
23	40.17193969898522
24	50.157350435369494
25	40.30842067495358
26	50.12272244038704
27	50.1003520198571
28	40.31740803437951
29	49.97143820557879
30	40.615272669084895
31	50.10739480886994
32	40.49315742594313
33	50.12223003523118
34	40.47345835756098
35	49.92168348200279
36	40.49507789063614
37	49.94682573480022
38	40.20222113023303
39	40.201290081201044
40	40.20064608529888
41	40.39179036710577
42	50.075826968004954
43	40.60875697171718
44	50.150487347136455
45	50.03962657178086
46	40.23311987748727
47	40.138433126245616
48	40.5015426468981
49	50.24438880400224
50	50.355431605466606
51	50.41780482847474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	77.5
2	70.0
3	94.0
4	118.0
5	300.5
6	483.0
7	932.0
8	1381.0
9	2460.0
10	3539.0
11	5837.5
12	8136.0
13	12047.0
14	15958.0
15	22466.5
16	28975.0
17	38309.0
18	47643.0
19	60977.0
20	74311.0
21	89939.5
22	105568.0
23	123071.0
24	140574.0
25	158123.5
26	193903.0
27	212133.0
28	228811.0
29	245489.0
30	260668.0
31	275847.0
32	282375.5
33	288904.0
34	292644.0
35	296384.0
36	295402.5
37	294421.0
38	292324.0
39	290227.0
40	288625.5
41	287024.0
42	283501.0
43	279978.0
44	275555.0
45	271132.0
46	264060.0
47	256988.0
48	260001.5
49	263015.0
50	558599.0
51	854183.0
52	544583.0
53	234983.0
54	218139.5
55	201296.0
56	197346.0
57	193396.0
58	191694.0
59	189992.0
60	187781.0
61	185570.0
62	181937.5
63	178305.0
64	172291.5
65	166278.0
66	157396.5
67	148515.0
68	136494.0
69	124473.0
70	110671.0
71	96869.0
72	83049.0
73	69229.0
74	57475.5
75	36541.0
76	27360.0
77	21111.0
78	14862.0
79	11110.0
80	7358.0
81	5386.5
82	3415.0
83	2418.5
84	1422.0
85	1002.5
86	583.0
87	402.0
88	221.0
89	158.0
90	95.0
91	70.0
92	45.0
93	26.5
94	8.0
95	7.5
96	7.0
97	5.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042840408356406776
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0014981280209411059
20	1.5132606272132383E-5
21	0.012348206718060023
22	0.0
23	0.0
24	0.0010138846202328697
25	0.0
26	0.09003900731918768
27	0.11323729273436663
28	0.12160562400285584
29	0.6852649424272428
30	0.13511904140387004
31	0.034018098899753595
32	0.11924493742440317
33	0.01563198227911275
34	0.011818565498535391
35	0.0164037451989915
36	0.029251327924031897
37	0.03946583715772125
38	0.008020281324230162
39	0.01613135828609312
40	0.025558971993631593
41	0.013422621763381421
42	0.01663073429307349
43	0.020792201017909896
44	0.015843838766922604
45	0.02374305924097571
46	0.019067083902886802
47	0.008776911637836782
48	0.031309362377041905
49	0.01124352646019436
50	0.014043058620538852
51	0.011137598216289433
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6608247.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.711394436519253
#Duplication Level	Relative count
1	100.0
2	14.990176458780665
3	2.504242674922902
4	0.8686078551833626
5	0.5450088503111294
6	0.4105814441517996
7	0.2712878875432631
8	0.22931733991885694
9	0.20498658767282438
10++	1.6295521316780313
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC	601977	9.109480925879435	TruSeq Adapter, Index 8 (97% over 36bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAGTC	1482680	5.3945565	111.49053	27
TTTTT	2222030	5.045308	5.326626	15
AGTCA	1519075	4.592379	92.53275	28
AAAAA	2200040	4.259908	4.4269285	15
GATCG	1066465	4.0514507	120.6892	1
TCCAG	1085330	3.9488451	112.765076	25
GGAAG	973255	3.739492	121.77352	5
TCTCG	993755	3.7326841	114.89874	41
ATCGG	968885	3.680749	120.636665	2
CGGAA	995195	3.6621697	116.9945	4
ATTTT	1663860	3.659493	3.884238	6
GAGCA	974600	3.5863836	116.03908	9
TCGGA	903815	3.433551	120.44403	3
GAAGA	1114630	3.408091	97.23402	6
CTGAA	1126940	3.4068992	94.63455	19
GTCTG	868590	3.406531	121.85279	17
ATGCC	917250	3.3373063	110.68144	47
TTTTC	1249265	3.3068044	3.6111765	1
AAAAT	1644555	3.287393	3.4963174	37
CTCCA	933785	3.2538655	107.58068	24
AGAGC	877290	3.228297	115.720314	8
CGTCT	857990	3.2227318	116.67905	16
CTCGT	857650	3.2214549	114.07886	42
TCAAA	1336535	3.2153885	73.585625	36
CCAGT	881140	3.205924	111.80622	26
AGCAC	905110	3.1898925	110.861404	10
CGGCG	567895	3.1604838	3.2894704	42
ACGTC	863855	3.1430347	113.26608	15
GTCAC	863465	3.1416159	111.63157	29
TCTGA	999670	3.1199584	97.4051	18
GCACA	880415	3.1028595	110.61982	11
AAATC	1271295	3.0584362	73.81023	38
CACGT	837350	3.0465994	113.30588	14
CACAG	862950	3.0413074	107.65087	31
AATTT	1416545	3.017873	3.1823146	5
TGAAC	980310	2.9636161	94.04492	20
GAACT	972955	2.9413805	93.967476	21
CAAAT	1219885	2.934756	73.60553	37
ACACG	826075	2.9113486	110.00678	13
CACAC	861235	2.9069715	105.60986	12
ATCTC	959405	2.8677316	91.20779	40
AAGAG	934995	2.8588398	96.51768	7
ACTCC	819485	2.8555763	107.62209	23
GTCAA	921445	2.7856588	91.602905	35
TCACA	927270	2.6847825	88.579	30
AACTC	920070	2.663936	89.860695	22
ACAGT	878460	2.655709	91.892525	32
GTATG	804195	2.620651	98.7228	45
TATGC	826710	2.5801523	94.51978	46
TCGTA	821050	2.5624874	94.632324	43
CGTAT	806835	2.5181227	94.53518	44
AATCT	997120	2.4764762	75.543884	39
TGCCG	458600	2.0967553	5.4471335	47
>>END_MODULE