FastQCFastQC Report
Wed 23 Jul 2014
LW193_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW193_CTTGTA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6284938
Filtered Sequences 0
Sequence length 51
%GC 38

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC 111405 1.7725711852686534 TruSeq Adapter, Index 12 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2681655 3.500712 3.6969447 14
TTTTT 2788620 3.4362564 3.705472 7
GAGCA 448445 2.2789516 30.901115 9
GGAAG 428600 2.2449975 31.992813 5
GAAGA 673760 2.220916 20.875399 6
CTCCA 467010 2.208337 28.521734 24
CGGAA 429990 2.1851654 31.074097 4
TCCAG 431530 2.1032352 29.167562 25
CTGAA 663665 2.0981023 19.742638 19
TCTCG 388135 1.8700285 28.391907 41
TCGGA 370410 1.8607893 30.393286 3
AGAGC 351570 1.7866429 30.4299 8
TCACC 373985 1.7684526 27.666286 30
GCACA 355555 1.7530535 29.563677 11
CACAC 363155 1.7371715 28.690605 12
AGCAC 346270 1.7072737 29.4977 10
TCTGA 532175 1.6631094 19.136799 18
ATCGG 330955 1.6625836 30.045404 2
CCAGT 325615 1.5870159 28.62371 26
AAGAG 473235 1.5599251 20.123272 7
CTCGT 322180 1.5522583 27.997477 42
CGTCT 316210 1.5234951 28.508528 16
CACGT 311655 1.5189762 28.77828 14
GATCG 300465 1.5094142 29.727627 1
GTCTG 303905 1.5091796 29.298162 17
GTCAC 307170 1.4971168 28.407068 29
TGAAC 473430 1.496696 19.234814 20
CACCT 312375 1.4771191 27.319038 31
CCTTG 306410 1.4762787 27.837545 33
ACACG 297615 1.467382 29.1771 13
ACTCC 306995 1.4516789 27.931623 23
ATGCC 295880 1.4420905 28.251486 47
ACGTC 290340 1.4150891 28.74215 15
CAGTC 283605 1.3822634 28.382874 27
AACTC 441180 1.3531812 18.560974 22
GAACT 419040 1.3247479 19.012886 21
CTTGT 421070 1.300796 18.150158 34
ATCTC 427275 1.2954963 17.980946 40
AGTCA 367820 1.1628218 18.630625 28
AATCT 556525 1.0944964 11.864416 39
ACCTT 346830 1.0515875 17.5958 32
TTGTA 512615 1.0271815 11.931511 35
TCGTA 328240 1.0257885 18.224747 43
TATGC 301800 0.9431605 18.111706 46
GTATG 287440 0.9258725 18.620962 45
CGTAT 294115 0.91914403 18.109377 44
TGTAA 449335 0.91083044 11.950182 36
GTAAT 387200 0.78487885 11.830854 37
TAATC 381690 0.75065506 11.432849 38
Produced by FastQC (version 0.10.1)