##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW193_CTTGTA_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6284938	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80136892360752	34.0	31.0	34.0	31.0	34.0
2	32.98695070659408	34.0	33.0	34.0	31.0	34.0
3	33.04704453727308	34.0	33.0	34.0	31.0	34.0
4	36.382336786774985	37.0	37.0	37.0	35.0	37.0
5	36.32421449503559	37.0	37.0	37.0	35.0	37.0
6	36.32404456495832	37.0	37.0	37.0	35.0	37.0
7	36.30042682998623	37.0	37.0	37.0	35.0	37.0
8	36.288333472820256	37.0	37.0	37.0	35.0	37.0
9	38.08348881086814	39.0	38.0	39.0	37.0	39.0
10	38.04473250173669	39.0	38.0	39.0	37.0	39.0
11	38.045777380779256	39.0	38.0	39.0	37.0	39.0
12	38.0291107088089	39.0	38.0	39.0	35.0	39.0
13	38.01081331271685	39.0	38.0	39.0	35.0	39.0
14	39.544584528916594	41.0	39.0	41.0	37.0	41.0
15	39.49931518815301	41.0	39.0	41.0	37.0	41.0
16	39.47389902652978	41.0	39.0	41.0	37.0	41.0
17	39.45201814242241	41.0	39.0	41.0	37.0	41.0
18	39.3917865219991	41.0	39.0	41.0	37.0	41.0
19	39.451181857323014	41.0	39.0	41.0	37.0	41.0
20	39.4331372879096	41.0	39.0	41.0	37.0	41.0
21	39.40273937467641	41.0	39.0	41.0	37.0	41.0
22	39.32653225855211	40.0	39.0	41.0	36.0	41.0
23	39.27169369053442	40.0	39.0	41.0	36.0	41.0
24	39.207440073394515	40.0	39.0	41.0	36.0	41.0
25	39.196099627394894	40.0	39.0	41.0	36.0	41.0
26	39.06879861026473	40.0	39.0	41.0	36.0	41.0
27	38.97920615923339	40.0	39.0	41.0	36.0	41.0
28	38.85397007894111	40.0	38.0	41.0	35.0	41.0
29	38.58986421186653	40.0	38.0	41.0	35.0	41.0
30	38.590992623952694	40.0	38.0	41.0	35.0	41.0
31	38.82622087918767	40.0	38.0	41.0	35.0	41.0
32	38.806550518079895	40.0	38.0	41.0	35.0	41.0
33	38.830605488868784	40.0	38.0	41.0	35.0	41.0
34	38.97702093481272	40.0	39.0	41.0	36.0	41.0
35	39.03361433318833	40.0	39.0	41.0	36.0	41.0
36	39.029860437764064	40.0	39.0	41.0	36.0	41.0
37	38.96237464872366	40.0	39.0	41.0	35.0	41.0
38	38.91150254783739	40.0	39.0	41.0	35.0	41.0
39	38.872845523694906	40.0	38.0	41.0	35.0	41.0
40	38.81647169789105	40.0	38.0	41.0	35.0	41.0
41	38.809801305915826	40.0	38.0	41.0	35.0	41.0
42	38.75910231731801	40.0	38.0	41.0	35.0	41.0
43	38.7103104278833	40.0	38.0	41.0	35.0	41.0
44	38.64490214541496	40.0	38.0	41.0	35.0	41.0
45	38.55820821144138	40.0	38.0	41.0	34.0	41.0
46	38.50466750825545	40.0	38.0	41.0	34.0	41.0
47	38.42244569477058	40.0	38.0	41.0	34.0	41.0
48	38.35852445958894	40.0	38.0	41.0	34.0	41.0
49	38.3035826288183	40.0	38.0	41.0	34.0	41.0
50	38.19368289711052	40.0	38.0	41.0	34.0	41.0
51	37.21553625509114	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	10.0
12	9.0
13	10.0
14	8.0
15	18.0
16	38.0
17	77.0
18	210.0
19	418.0
20	852.0
21	1457.0
22	2430.0
23	4066.0
24	6152.0
25	9057.0
26	13331.0
27	18988.0
28	26206.0
29	35322.0
30	47081.0
31	61088.0
32	78658.0
33	103247.0
34	139355.0
35	198726.0
36	307120.0
37	539444.0
38	1236932.0
39	3452726.0
40	1895.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.54842265566389	27.67249485170179	32.05016799123488	21.728914501399434
2	18.4266893324962	31.31017362462446	30.62404115999235	19.63909588288699
3	18.551479744112033	29.84043756676677	32.47102835381988	19.13705433530132
4	18.627518680375207	28.89107259291977	31.1917953685462	21.289613358158824
5	20.846347251158246	29.70979188657072	31.394168088849884	18.049692773421153
6	20.83861447797894	30.27719605189423	29.778177604934207	19.106011865192624
7	19.322800002163902	31.999536033609242	30.576085237435912	18.10157872679094
8	18.54113755776111	32.372857138765724	30.461382435276214	18.62462286819695
9	20.262093277610692	29.821598876552162	30.912747269742358	19.003560576094785
10	18.560676334436394	31.880171292063658	30.39306036113642	19.166092012363528
11	20.49577895597379	29.824701532457443	30.390435036908876	19.289084474659894
12	18.580978841796053	29.905816095560528	30.265946935355608	21.24725812728781
13	18.499196014344136	32.05850240686543	30.31721235754434	19.125089221246096
14	18.516173111015576	30.23361566971703	30.312852728221024	20.93735849104637
15	18.44718277252695	32.14394477717998	30.421318396458325	18.98755405383474
16	18.50314195621341	30.148523342632817	30.430260409887893	20.91807429126588
17	20.485579969126185	30.056684727836615	30.45745240446286	19.00028289857434
18	18.60966647562792	29.949380566681803	32.30547381692548	19.135479140764794
19	18.611542617454628	29.940184549115163	30.345461954346902	21.102810879083304
20	18.69745074612522	29.845708875045208	32.25155637996053	19.205283998869042
21	20.655180639261093	29.79499527298981	30.322230760527486	19.227593327221612
22	18.70601684822516	31.810289207446058	30.275205710162478	19.208488234166303
23	18.73754363209311	31.871945276150697	30.2583414506237	19.1321696411325
24	18.708491570546908	29.91313421379733	30.26154248585651	21.11683172979926
25	18.686723719470262	29.949571499352896	32.20101773478115	19.162687046395686
26	18.692588391123646	29.945251786519396	30.295158118771308	21.067001703585653
27	18.79677198345945	29.92578607676137	30.1779977676406	21.099444172138583
28	18.805751395751372	31.8775833049895	30.121449685642297	19.19521561361683
29	20.79362876713997	29.943134365571932	30.12607929012818	19.13715757715991
30	18.879609143896523	29.871927197569768	31.993743421654315	19.254720236879397
31	18.84532379310844	29.87890138858404	30.12605505472063	21.149719763586887
32	18.860052137463974	31.8397763862276	30.07046714071699	19.229704335591432
33	18.862164721234258	29.94901296159263	30.06276307099834	21.126059246174776
34	18.826881081682338	29.98702924522492	30.10527937248981	21.08081030060293
35	18.833498544765508	30.025333126878717	32.02938572697354	19.111782601382238
36	18.828755816463016	29.977074748035506	32.04730266998651	19.14686676551497
37	20.74576264711533	30.022645800546638	30.125695574032925	19.10589597830511
38	18.862769210009663	30.056014840543405	31.99331806379155	19.087897885655384
39	18.881384852058687	31.924829209737876	30.078771433622904	19.115014504580532
40	18.865638747239313	31.89786843138494	30.091797201696497	19.144695619679254
41	18.877281470834983	29.954405592269	32.00589932948277	19.162413607413246
42	18.927994497655664	29.929500417497696	30.079058006797403	21.063447078049233
43	18.86300875423962	29.960608468033527	31.96683612409733	19.209546653629523
44	18.873876834386472	29.974062339819152	30.14236511549412	21.009695710300257
45	20.77350195904366	29.9874288266125	30.159277833117393	19.079791381226443
46	18.82423955776458	30.05565818420704	32.03428164753572	19.08582061049265
47	18.872500150771863	31.966992816575964	30.1413245550957	19.019182477556473
48	18.90539883678771	30.04023908529534	31.959879967136434	19.09448211078052
49	20.78609104795325	30.037588020590743	30.072612462113213	19.10370846934279
50	18.937148344077254	29.983868603834583	30.081449467291556	20.99753358479661
51	18.939639624443565	29.94851232181167	30.074685458279482	21.03716259546528
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	40.27733715706333
2	38.065785215383194
3	37.68853407941336
4	39.91713203853403
5	38.8960400245794
6	39.944626343171564
7	37.42437872895484
8	37.16576042595806
9	39.26565385370548
10	37.72676834679992
11	39.784863430633685
12	39.82823696908386
13	37.624285235590236
14	39.45353160206195
15	37.43473682636169
16	39.42121624747929
17	39.48586286770052
18	37.74514561639272
19	39.71435349653794
20	37.90273474499426
21	39.8827739664827
22	37.914505082391464
23	37.86971327322561
24	39.82532330034616
25	37.84941076586595
26	39.759590094709296
27	39.89621615559803
28	38.0009670093682
29	39.93078634429988
30	38.13432938077592
31	39.99504355669533
32	38.08975647305541
33	39.988223967409034
34	39.90769138228527
35	37.94528114614775
36	37.975622581977994
37	39.85165862542044
38	37.95066709566505
39	37.99639935663922
40	38.01033436691857
41	38.03969507824823
42	39.9914415757049
43	38.07255540786914
44	39.88357254468672
45	39.8532933402701
46	37.91006016825723
47	37.89168262832834
48	37.99988094756823
49	39.88979951729604
50	39.93468192887386
51	39.97680221990885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	36.0
2	36.0
3	72.0
4	108.0
5	235.5
6	363.0
7	750.5
8	1138.0
9	2222.0
10	3306.0
11	5685.5
12	8065.0
13	12734.5
14	17404.0
15	25409.5
16	33415.0
17	46015.5
18	58616.0
19	76203.0
20	93790.0
21	115907.5
22	138025.0
23	163588.5
24	189152.0
25	215693.5
26	266807.5
27	291380.0
28	313401.0
29	335422.0
30	354577.5
31	373733.0
32	386656.0
33	399579.0
34	411229.5
35	422880.0
36	430666.0
37	438452.0
38	440340.5
39	442229.0
40	438431.0
41	434633.0
42	424787.0
43	414941.0
44	398502.0
45	382063.0
46	355085.0
47	328107.0
48	303317.0
49	278527.0
50	306551.5
51	334576.0
52	253030.5
53	171485.0
54	148355.5
55	125226.0
56	108038.5
57	90851.0
58	79418.0
59	67985.0
60	58543.0
61	49101.0
62	42394.5
63	35688.0
64	30597.5
65	25507.0
66	22067.0
67	18627.0
68	16040.5
69	13454.0
70	11540.5
71	9627.0
72	7907.0
73	6187.0
74	5119.0
75	3219.0
76	2387.0
77	1863.0
78	1339.0
79	1008.5
80	678.0
81	489.5
82	301.0
83	221.5
84	142.0
85	99.5
86	57.0
87	37.5
88	18.0
89	12.5
90	7.0
91	5.5
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04399406963123582
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0017024829839212417
20	1.5911055924497584E-5
21	0.013349375920653474
22	4.773316777349275E-5
23	0.0
24	0.0011137739147148308
25	0.0
26	0.09274554498389642
27	0.11575293185071994
28	0.12494952217507953
29	0.7042551573301121
30	0.13785338852984708
31	0.03511570042536617
32	0.12229237583568843
33	0.015720123253403613
34	0.012744755795522566
35	0.017040740895136915
36	0.030390116815790385
37	0.040366348880450376
38	0.008464681751832716
39	0.016881630335891937
40	0.02595093221285556
41	0.013269820641030985
42	0.017040740895136915
43	0.02154356972176973
44	0.016308832322610024
45	0.02410524972561384
46	0.01950695456343404
47	0.009689833058019029
48	0.03169482340159919
49	0.012012847222995676
50	0.01462226039461328
51	0.01139231604194027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6284938.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.91438095795075
#Duplication Level	Relative count
1	100.0
2	5.644636282570995
3	0.9431433369986816
4	0.5530846367202246
5	0.4595361966814922
6	0.36434655594032594
7	0.2899454574299891
8	0.2111678237131619
9	0.14661392941742846
10++	0.8009059427877435
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC	111405	1.7725711852686534	TruSeq Adapter, Index 12 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2681655	3.500712	3.6969447	14
TTTTT	2788620	3.4362564	3.705472	7
GAGCA	448445	2.2789516	30.901115	9
GGAAG	428600	2.2449975	31.992813	5
GAAGA	673760	2.220916	20.875399	6
CTCCA	467010	2.208337	28.521734	24
CGGAA	429990	2.1851654	31.074097	4
TCCAG	431530	2.1032352	29.167562	25
CTGAA	663665	2.0981023	19.742638	19
TCTCG	388135	1.8700285	28.391907	41
TCGGA	370410	1.8607893	30.393286	3
AGAGC	351570	1.7866429	30.4299	8
TCACC	373985	1.7684526	27.666286	30
GCACA	355555	1.7530535	29.563677	11
CACAC	363155	1.7371715	28.690605	12
AGCAC	346270	1.7072737	29.4977	10
TCTGA	532175	1.6631094	19.136799	18
ATCGG	330955	1.6625836	30.045404	2
CCAGT	325615	1.5870159	28.62371	26
AAGAG	473235	1.5599251	20.123272	7
CTCGT	322180	1.5522583	27.997477	42
CGTCT	316210	1.5234951	28.508528	16
CACGT	311655	1.5189762	28.77828	14
GATCG	300465	1.5094142	29.727627	1
GTCTG	303905	1.5091796	29.298162	17
GTCAC	307170	1.4971168	28.407068	29
TGAAC	473430	1.496696	19.234814	20
CACCT	312375	1.4771191	27.319038	31
CCTTG	306410	1.4762787	27.837545	33
ACACG	297615	1.467382	29.1771	13
ACTCC	306995	1.4516789	27.931623	23
ATGCC	295880	1.4420905	28.251486	47
ACGTC	290340	1.4150891	28.74215	15
CAGTC	283605	1.3822634	28.382874	27
AACTC	441180	1.3531812	18.560974	22
GAACT	419040	1.3247479	19.012886	21
CTTGT	421070	1.300796	18.150158	34
ATCTC	427275	1.2954963	17.980946	40
AGTCA	367820	1.1628218	18.630625	28
AATCT	556525	1.0944964	11.864416	39
ACCTT	346830	1.0515875	17.5958	32
TTGTA	512615	1.0271815	11.931511	35
TCGTA	328240	1.0257885	18.224747	43
TATGC	301800	0.9431605	18.111706	46
GTATG	287440	0.9258725	18.620962	45
CGTAT	294115	0.91914403	18.109377	44
TGTAA	449335	0.91083044	11.950182	36
GTAAT	387200	0.78487885	11.830854	37
TAATC	381690	0.75065506	11.432849	38
>>END_MODULE