##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW192_GGCTAC_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6259067	
Filtered Sequences	0	
Sequence length	51	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.815117173214475	34.0	31.0	34.0	31.0	34.0
2	33.006021184946576	34.0	33.0	34.0	31.0	34.0
3	33.06845173569799	34.0	33.0	34.0	31.0	34.0
4	36.39368663093077	37.0	37.0	37.0	35.0	37.0
5	36.34015277356833	37.0	37.0	37.0	35.0	37.0
6	36.34210146656043	37.0	37.0	37.0	35.0	37.0
7	36.332519047966734	37.0	37.0	37.0	35.0	37.0
8	36.31740896846127	37.0	37.0	37.0	35.0	37.0
9	38.11808660939402	39.0	39.0	39.0	37.0	39.0
10	38.08960952167472	39.0	38.0	39.0	37.0	39.0
11	38.08094976455756	39.0	38.0	39.0	37.0	39.0
12	38.06743177537483	39.0	38.0	39.0	37.0	39.0
13	38.04958039273266	39.0	38.0	39.0	37.0	39.0
14	39.586722749572736	41.0	40.0	41.0	37.0	41.0
15	39.55730414772681	41.0	40.0	41.0	37.0	41.0
16	39.51675129855616	41.0	39.0	41.0	37.0	41.0
17	39.501328872178554	41.0	39.0	41.0	37.0	41.0
18	39.45569283728709	41.0	39.0	41.0	37.0	41.0
19	39.50566849659862	41.0	39.0	41.0	37.0	41.0
20	39.48630954102265	41.0	39.0	41.0	37.0	41.0
21	39.458854171076936	41.0	39.0	41.0	37.0	41.0
22	39.404046162151644	41.0	39.0	41.0	37.0	41.0
23	39.34407092942127	40.0	39.0	41.0	36.0	41.0
24	39.27007779274451	40.0	39.0	41.0	36.0	41.0
25	39.26142969870749	40.0	39.0	41.0	36.0	41.0
26	39.12860686744526	40.0	39.0	41.0	36.0	41.0
27	39.04381435763509	40.0	39.0	41.0	36.0	41.0
28	38.97329921536229	40.0	39.0	41.0	36.0	41.0
29	38.683836744358224	40.0	38.0	41.0	35.0	41.0
30	38.71436573534043	40.0	38.0	41.0	35.0	41.0
31	38.92731392713962	40.0	39.0	41.0	36.0	41.0
32	38.91913459306315	40.0	39.0	41.0	35.0	41.0
33	38.92808209274641	40.0	38.0	41.0	35.0	41.0
34	39.06940091869922	40.0	39.0	41.0	36.0	41.0
35	39.12656678703072	40.0	39.0	41.0	36.0	41.0
36	39.119036910772806	40.0	39.0	41.0	36.0	41.0
37	39.06215127590102	40.0	39.0	41.0	36.0	41.0
38	39.022092749606294	40.0	39.0	41.0	35.0	41.0
39	38.9954004326843	40.0	39.0	41.0	35.0	41.0
40	38.94174547101029	40.0	39.0	41.0	35.0	41.0
41	38.92606262243238	40.0	39.0	41.0	35.0	41.0
42	38.876330609657956	40.0	39.0	41.0	35.0	41.0
43	38.82906398030249	40.0	38.0	41.0	35.0	41.0
44	38.76333773068734	40.0	38.0	41.0	35.0	41.0
45	38.68867181003175	40.0	38.0	41.0	35.0	41.0
46	38.6541532468018	40.0	38.0	41.0	35.0	41.0
47	38.57847024165103	40.0	38.0	41.0	35.0	41.0
48	38.50304765870057	40.0	38.0	41.0	34.0	41.0
49	38.45112106964185	40.0	38.0	41.0	34.0	41.0
50	38.33936463693391	40.0	38.0	41.0	34.0	41.0
51	37.38445602195982	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	2.0
13	8.0
14	9.0
15	18.0
16	28.0
17	81.0
18	174.0
19	382.0
20	724.0
21	1215.0
22	2074.0
23	3466.0
24	5299.0
25	8267.0
26	11998.0
27	17512.0
28	24172.0
29	32624.0
30	43667.0
31	56781.0
32	74064.0
33	97733.0
34	132136.0
35	188327.0
36	290341.0
37	508073.0
38	1189356.0
39	3568341.0
40	2187.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.480728587737072	27.97963222795844	32.941863041182785	21.597776143121706
2	18.51357398794421	31.05509175728587	30.14083408917016	20.290500165599763
3	19.480778844514685	31.28656395593784	30.243916545389272	18.9887406541582
4	18.801779881889743	30.27125288801031	31.175125621758003	19.751841608341948
5	19.114765826919573	30.784013016636507	32.0276648260835	18.073556330360418
6	19.138171871302863	31.303595248301384	30.56926855072809	18.988964329667667
7	19.10895026367348	31.368445169224103	31.29014276408928	18.232461803013134
8	18.63701091552463	31.883314238368115	30.849278334933945	18.630396511173313
9	18.887096111928503	30.914032394924035	31.144753682937086	19.05411781021037
10	18.556455778473055	31.438215951355048	30.845140338008843	19.160187932163055
11	19.03761375297628	30.82841260526529	30.88455515814098	19.249418483617447
12	18.57160180582825	30.898279248328862	30.839372705229067	19.690746240613816
13	18.442061732203857	31.537432016624845	30.945874201378576	19.074632049792726
14	18.47363193268262	31.119126860281256	30.992031240438873	19.415209966597256
15	18.393252540674194	31.570967366222476	31.102111544739813	18.93366854836352
16	18.408558336250437	31.026621060295405	31.151623716442085	19.413196887012074
17	18.925456461801733	30.958703589528596	31.181883817508265	18.933956131161402
18	18.497884748637457	30.892767244702764	31.65078756945724	18.958560437202543
19	18.49681632869567	30.860545882237155	31.10742360283299	19.53521418623419
20	18.5428275492178	30.891952426775426	31.565343524841644	18.99987649916513
21	19.05600411614035	30.83903110913684	31.081557422235147	19.02340735248766
22	18.548728923569254	31.405377640270554	31.082054588025525	18.963838848134664
23	18.584191541646703	31.466079529105535	31.014334884096943	18.93539404515082
24	18.574352129658905	30.971110912641574	31.051507052156996	19.403029905542525
25	18.52159435264074	30.99597751549872	31.563809749919596	18.91861838194095
26	18.53921776671038	31.04955813658579	31.041754385572144	19.369469711131686
27	18.58094117997064	31.04030820202742	31.01020584922944	19.3685447687725
28	18.572487314338073	31.553493493964265	30.956422365796566	18.917596825901096
29	19.084194126940144	31.0552674711575	30.963950456134526	18.89658794576783
30	18.659227261819055	30.99684825213983	31.447148228141746	18.89677625789937
31	18.628213588598967	30.974169825442505	30.981441789050084	19.41617479690844
32	18.59316827714135	31.484798682399973	31.00040855070716	18.921624489751522
33	18.6456205075891	31.015248510159864	31.01625521288247	19.32287576936856
34	19.06728670933335	31.069356287886812	31.02260222019504	18.8407547825848
35	19.08059106001828	31.13237712442855	30.99097360057412	18.79605821497906
36	18.590790594083458	31.121027915504268	31.043772518174357	19.24440897223792
37	18.56795930292774	31.10754969817318	31.54748737646582	18.77700362243326
38	18.609729620559946	31.60845232544725	31.005148486755214	18.77666956723759
39	18.61147735068145	31.09943086077813	31.023640442457527	19.26545134608289
40	18.602407897989785	31.568659067987475	31.01380409496394	18.815128939058802
41	18.62284482448685	31.076360176784274	31.466469295165467	18.834325703563408
42	18.636966935516604	31.065718293375504	31.006482248202065	19.29083252290583
43	18.630191275277717	31.072744371565754	31.468894096665966	18.828170256490566
44	18.61949517601343	31.096025600094084	31.01429155357327	19.27018767031921
45	19.128324228907058	31.12251559269168	30.99446287453982	18.754697303861445
46	18.568700118762568	31.17715565566781	31.51492266370762	18.739221561862003
47	18.635753119844	31.65227181142532	31.02218332037493	18.689791748355752
48	18.66350969944351	31.131473887097133	31.507401239559798	18.697615173899564
49	19.154266616472057	31.083385477352664	31.02228270005247	18.740065206122804
50	18.698997580598384	31.080242608089698	30.971904037593163	19.248855773718756
51	18.714555523953482	31.053387617881945	30.945899737631784	19.286157120532792
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.07850473085878
2	38.80407415354397
3	38.46951949867288
4	38.55362149023169
5	37.18832215727999
6	38.12713620097053
7	37.341412066686615
8	37.26740742669794
9	37.94121392213887
10	37.716643710636106
11	38.287032236593724
12	38.262348046442064
13	37.51669378199658
14	37.88884189927988
15	37.32692108903771
16	37.82175522326251
17	37.85941259296314
18	37.456445185839996
19	38.03203051492986
20	37.54270404838293
21	38.07941146862801
22	37.51256777170392
23	37.51958558679752
24	37.97738203520143
25	37.44021273458169
26	37.908687477842065
27	37.949485948743146
28	37.49008414023917
29	37.980782072707974
30	37.55600351971842
31	38.04438838550741
32	37.51479276689287
33	37.968496276957666
34	37.90804149191814
35	37.876649274997334
36	37.83519956632138
37	37.344962925360996
38	37.38639918779753
39	37.87692869676434
40	37.41753683704859
41	37.45717052805026
42	37.927799458422434
43	37.45836153176828
44	37.88968284633264
45	37.8830215327685
46	37.307921680624574
47	37.32554486819975
48	37.36112487334307
49	37.894331822594864
50	37.94785335431714
51	38.00071264448627
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	57.0
2	55.0
3	104.0
4	153.0
5	342.0
6	531.0
7	1083.5
8	1636.0
9	3113.0
10	4590.0
11	7718.5
12	10847.0
13	16501.5
14	22156.0
15	31704.5
16	41253.0
17	55456.0
18	69659.0
19	90277.5
20	110896.0
21	135453.0
22	160010.0
23	187578.0
24	215146.0
25	243230.0
26	295806.0
27	320298.0
28	342049.5
29	363801.0
30	379690.5
31	395580.0
32	406375.5
33	417171.0
34	425518.5
35	433866.0
36	438117.0
37	442368.0
38	439838.5
39	437309.0
40	431168.5
41	425028.0
42	412629.0
43	400230.0
44	381330.0
45	362430.0
46	336616.5
47	310803.0
48	283891.5
49	256980.0
50	233062.5
51	209145.0
52	185295.5
53	161446.0
54	153686.5
55	145927.0
56	116775.5
57	87624.0
58	75272.5
59	62921.0
60	53526.5
61	44132.0
62	36491.0
63	28850.0
64	23439.0
65	18028.0
66	14690.0
67	11352.0
68	9059.0
69	6766.0
70	5403.0
71	4040.0
72	3118.0
73	2196.0
74	1710.0
75	915.0
76	606.0
77	457.5
78	309.0
79	236.5
80	164.0
81	124.0
82	84.0
83	61.5
84	39.0
85	29.5
86	20.0
87	16.5
88	13.0
89	8.0
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04647657550238718
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0015657285662543634
20	0.0
21	0.012413990775302452
22	3.195364420927273E-5
23	0.0
24	9.586093262781817E-4
25	0.0
26	0.09049272040066035
27	0.11286027134715126
28	0.12330911300358345
29	0.7102176730174002
30	0.1368734349704197
31	0.03450993574601455
32	0.12303750702780464
33	0.016184520791996636
34	0.012589735818453452
35	0.017414736094053633
36	0.030339985176704453
37	0.03992607843948627
38	0.008451738893352635
39	0.01629635854672909
40	0.025179471636906904
41	0.013069040481592544
42	0.01682359367618209
43	0.021281127043375633
44	0.01541763333097409
45	0.023773511291698905
46	0.019220116991877544
47	0.009282533642793725
48	0.032065481964005174
49	0.012126407977418999
50	0.014506954471009815
51	0.010976076785885181
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6259067.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	9.87467406846665
#Duplication Level	Relative count
1	100.0
2	5.570530592078176
3	1.04800856413494
4	0.6818368971480333
5	0.4990255551590678
6	0.3755043781394966
7	0.322253026268837
8	0.26186489528149104
9	0.19763388323131398
10++	0.8399440037330844
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC	26567	0.42445623285387424	TruSeq Adapter, Index 11 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3102545	3.638159	3.903042	3
TTTTT	3095065	3.6122901	3.785008	15
TTTTC	1613325	3.0661404	3.2621236	4
GAAAA	1570575	3.0622163	3.3603585	3
GAAGA	583510	1.8916428	6.547647	6
CTGAA	585210	1.8544964	6.3411713	19
CGGAA	346555	1.8277217	9.535324	4
CTCCA	360520	1.8185464	8.92329	24
GAGCA	339730	1.7917273	9.331051	9
GGAAG	330265	1.7801924	9.645492	5
TCCAG	335900	1.7316948	8.931194	25
TCTCG	287070	1.4785604	8.453765	41
TCGGA	275280	1.450449	9.030341	3
TCTGA	449195	1.422129	5.900352	18
CGGCT	158735	1.3606539	12.789788	33
CACAC	269085	1.3586094	8.601146	12
GCACA	256590	1.3240709	8.691355	11
ACGGC	151920	1.303467	12.735855	32
AGAGC	246080	1.2978196	8.863075	8
AAGAG	380465	1.2334044	5.884054	7
TGAAC	386810	1.2257783	5.69603	20
ATCGG	232310	1.2240403	8.734732	2
CCAGT	237200	1.2228582	8.368121	26
AGCAC	236735	1.221614	8.622309	10
CACGG	141720	1.2159516	12.806116	31
CTCGT	219600	1.1310548	8.103606	42
CGTCT	217470	1.120084	8.433319	16
GTCAC	215930	1.113203	8.110952	29
CACGT	213865	1.1025571	8.4350605	14
GTCTG	208805	1.0991541	8.518995	17
AACTC	350640	1.0871997	5.480659	22
CATCT	347305	1.0758425	5.1863747	39
ACTCC	209760	1.058078	8.142467	23
GAACT	333785	1.0577452	5.5442553	21
ATCTC	330955	1.0251954	5.206821	40
ATGCC	198485	1.0232673	7.9096622	47
ACACG	197740	1.0203898	8.370351	13
TCACG	197200	1.0166427	7.9783688	30
GATCG	190880	1.0057459	8.354105	1
ACGTC	191485	0.9871795	8.299691	15
CAGTC	188660	0.97261554	8.097546	27
ACATC	301285	0.9341688	5.0251822	38
AGTCA	283700	0.8990287	5.2044387	28
GCTAC	151045	0.7786956	7.533114	35
GGCTA	127270	0.670585	7.6619473	34
>>END_MODULE