FastQCFastQC Report
Wed 23 Jul 2014
LW190_GATCAG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW190_GATCAG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6042676
Filtered Sequences 0
Sequence length 51
%GC 37

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC 138092 2.285278906232934 TruSeq Adapter, Index 9 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3013165 3.70001 3.943509 5
AAAAA 3006825 3.5952463 3.9979515 3
TTTTC 1541620 3.0727687 3.2868805 4
GAAAA 1504890 3.001638 3.4497545 2
CGGAA 445240 2.4174929 40.705227 4
GGAAG 434680 2.4126217 41.486324 5
GAGCA 440430 2.391376 40.478355 9
CTCCA 456695 2.385679 38.340652 24
TCCAG 435710 2.326654 39.02992 25
GAAGA 671510 2.2342875 25.566467 6
CTGAA 681925 2.2314465 24.911064 19
TCTCG 390035 2.0938704 38.677826 41
TCGGA 378550 2.066361 40.50349 3
GCACA 365160 1.9395708 39.073177 11
CACAC 370845 1.9269316 38.128338 12
AGAGC 352700 1.9150339 39.96599 8
ATCGG 338125 1.8456963 40.07424 2
AGCAC 345445 1.8348533 39.019566 10
TCAGA 559515 1.8308873 23.845114 36
TCTGA 553840 1.8219903 24.683342 18
CCAGT 340460 1.8180274 38.418896 26
CACGA 333800 1.7730001 37.82265 31
GTCTG 318925 1.7501825 39.8547 17
GTCAC 327535 1.7490088 38.33936 29
CTCGT 325525 1.7475538 38.23951 42
CGTCT 322980 1.7338911 38.95899 16
CACGT 319525 1.7062362 38.785355 14
ACTCC 317270 1.6573522 37.70424 23
GATCG 301815 1.6474934 39.840305 1
ATGCC 307530 1.6421841 38.05601 47
ACACG 308755 1.6399722 38.596893 13
TGAAC 498810 1.6322438 24.335428 20
TCACG 305190 1.6296884 38.08363 30
ACGTC 299745 1.6006128 38.681957 15
CGATC 298985 1.5965544 37.63794 33
CAGTC 298070 1.5916684 38.18534 27
AAGAG 477980 1.590363 24.92211 7
AACTC 453815 1.4527142 23.580727 22
GAACT 440450 1.4412738 24.071922 21
ATCAG 435385 1.4246999 23.379322 35
CAGAT 428635 1.4026119 23.364056 37
GATCA 428205 1.4012047 23.389214 34
ATCTC 435285 1.4008349 23.292152 40
AGTCA 397595 1.3010404 23.731688 28
AGATC 388020 1.2697084 23.362928 38
GATCT 383465 1.2615006 23.498968 39
ACGAT 379260 1.2410432 23.451145 32
TCGTA 328425 1.0804332 23.507912 43
TATGC 318950 1.0492631 23.471275 46
GTATG 311505 1.0475513 23.982693 45
CGTAT 300075 0.9871691 23.417772 44
Produced by FastQC (version 0.10.1)