##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW190_GATCAG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6042676	
Filtered Sequences	0	
Sequence length	51	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81792255616551	34.0	31.0	34.0	31.0	34.0
2	33.000736097715645	34.0	33.0	34.0	31.0	34.0
3	33.06259743199867	34.0	33.0	34.0	31.0	34.0
4	36.39654798635571	37.0	37.0	37.0	35.0	37.0
5	36.34379040014722	37.0	37.0	37.0	35.0	37.0
6	36.344259232168	37.0	37.0	37.0	35.0	37.0
7	36.32939628072066	37.0	37.0	37.0	35.0	37.0
8	36.32208296456735	37.0	37.0	37.0	35.0	37.0
9	38.11657914473654	39.0	39.0	39.0	37.0	39.0
10	38.08717180931097	39.0	38.0	39.0	37.0	39.0
11	38.08231022149789	39.0	38.0	39.0	37.0	39.0
12	38.07233235738603	39.0	38.0	39.0	37.0	39.0
13	38.05305927373899	39.0	38.0	39.0	37.0	39.0
14	39.60770559268774	41.0	40.0	41.0	37.0	41.0
15	39.55413760393574	41.0	40.0	41.0	37.0	41.0
16	39.53581956073766	41.0	39.0	41.0	37.0	41.0
17	39.517368794884916	41.0	39.0	41.0	37.0	41.0
18	39.46303789910298	41.0	39.0	41.0	37.0	41.0
19	39.5238662473381	41.0	39.0	41.0	37.0	41.0
20	39.504275589159505	41.0	39.0	41.0	37.0	41.0
21	39.47480338181296	41.0	39.0	41.0	37.0	41.0
22	39.395801462795625	41.0	39.0	41.0	37.0	41.0
23	39.3433930596312	40.0	39.0	41.0	36.0	41.0
24	39.291678057867074	40.0	39.0	41.0	36.0	41.0
25	39.27817807871877	40.0	39.0	41.0	36.0	41.0
26	39.16912457328508	40.0	39.0	41.0	36.0	41.0
27	39.09085411827475	40.0	39.0	41.0	36.0	41.0
28	38.94620727637888	40.0	39.0	41.0	36.0	41.0
29	38.69795236415125	40.0	38.0	41.0	35.0	41.0
30	38.68827751148663	40.0	38.0	41.0	35.0	41.0
31	38.93035105638628	40.0	39.0	41.0	36.0	41.0
32	38.91314576522058	40.0	39.0	41.0	35.0	41.0
33	38.95344546025635	40.0	39.0	41.0	36.0	41.0
34	39.08561918593683	40.0	39.0	41.0	36.0	41.0
35	39.09044055977848	40.0	39.0	41.0	36.0	41.0
36	39.12705082979792	40.0	39.0	41.0	36.0	41.0
37	39.050958383338774	40.0	39.0	41.0	36.0	41.0
38	39.025796683456136	40.0	39.0	41.0	35.0	41.0
39	39.015009740717524	40.0	39.0	41.0	35.0	41.0
40	38.932441355452454	40.0	39.0	41.0	35.0	41.0
41	38.93478932181702	40.0	39.0	41.0	35.0	41.0
42	38.90374545979298	40.0	39.0	41.0	35.0	41.0
43	38.86520309875956	40.0	38.0	41.0	35.0	41.0
44	38.81314156178488	40.0	38.0	41.0	35.0	41.0
45	38.73427501325572	40.0	38.0	41.0	35.0	41.0
46	38.66840767236238	40.0	38.0	41.0	35.0	41.0
47	38.60001098850906	40.0	38.0	41.0	35.0	41.0
48	38.54527447773139	40.0	38.0	41.0	34.0	41.0
49	38.496596871981886	40.0	38.0	41.0	34.0	41.0
50	38.39832815792209	40.0	38.0	41.0	34.0	41.0
51	37.440541409137275	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	2.0
12	5.0
13	2.0
14	7.0
15	9.0
16	27.0
17	63.0
18	145.0
19	356.0
20	707.0
21	1148.0
22	1945.0
23	3285.0
24	5141.0
25	7864.0
26	11644.0
27	16637.0
28	23656.0
29	31903.0
30	41958.0
31	55234.0
32	71717.0
33	93883.0
34	126844.0
35	180170.0
36	276782.0
37	483025.0
38	1127857.0
39	3478260.0
40	2393.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.157671585025422	27.437875650473515	31.257182298809127	21.147270465691935
2	18.76056237335909	32.37572227933452	29.517005379735732	19.346709967570657
3	18.77979226422201	31.053923791379844	32.018479892021354	18.147804052376795
4	18.129054081337472	30.318603876825435	30.728223720748886	20.824118321088207
5	20.555479062587505	30.727661056128113	31.371084598942588	17.345775282341798
6	20.516075990173892	30.914730493576027	30.404741210682158	18.16445230556793
7	18.338745946332384	33.13018933995468	30.940910947401452	17.59015376631148
8	17.97731667228228	33.40940338353405	30.60087947790019	18.01240046628348
9	20.383187845914623	30.385792652129616	30.885405737458036	18.345613764497713
10	18.032937724941732	32.98025576747785	30.506699349758286	18.480107157822133
11	20.572077668900334	30.38195329354081	30.485152604574527	18.560816432984325
12	18.03581724388334	30.425361214137574	30.458210898615118	21.08061064336397
13	17.946618352531228	33.13755362690305	30.452153979462082	18.463674041103644
14	17.923036085337028	30.706925209956648	30.517389977552988	20.852648727153333
15	17.87590464886749	33.17134660206836	30.607830040862687	18.344918708201465
16	17.887108294404662	30.566590033951847	30.653405875145385	20.892895796498106
17	20.468199850529796	30.507841227959272	30.663600034157053	18.360358887353883
18	18.02299180032158	30.44253903403062	33.14534818679671	18.389120978851093
19	18.031410046256664	30.349855004204322	30.60641721619657	21.01231773334245
20	18.112091397917084	30.37094823551685	33.04562415724424	18.47133620932183
21	20.622465694656416	30.363251742887066	30.549502858647603	18.46477970380892
22	18.094293700817502	32.95793103482515	30.49867169711153	18.44910356724582
23	18.092878056013596	33.019327860702774	30.52662429691746	18.361169786366172
24	18.05529122953579	30.509801870777288	30.531481201372518	20.903425698314408
25	18.02780754751703	30.50979400517254	33.081733986730384	18.38066446058005
26	18.051839157417405	30.488410083189528	30.583340428400724	20.87641033099235
27	18.096921575085773	30.511786236655198	30.46677062616267	20.92452156209636
28	18.136529974308562	33.04482041263711	30.41300992446668	18.405639688587648
29	20.656257396104525	30.555437032691195	30.428915726909988	18.35938984429429
30	18.174304954123212	30.50398014254071	32.940771329318224	18.38094357401786
31	18.14685480231619	30.502585336636756	30.436317150961344	20.91424271008571
32	18.148818913294186	33.04676626148279	30.394374925436445	18.410039899786582
33	18.163751447844014	30.548126682668673	30.453253382881957	20.834868486605355
34	20.63250130587023	30.521894594259997	30.490034008899116	18.35557009097066
35	18.145628477527985	33.10576849230541	30.490985241113023	18.257617789053583
36	18.102170907054468	30.59795170836369	32.997287297343966	18.30259008723788
37	18.109283159859984	30.620704521046182	30.50829134118857	20.761720977905266
38	18.144274581372606	33.1387790546701	30.45054712805148	18.266399235905805
39	20.640955678512178	30.61803662116786	30.47604009270905	18.264967607610906
40	18.16317003969502	33.0565496783677	30.459472344257467	18.320807937679813
41	18.129158569725934	30.608364169429734	32.95991271606853	18.302564544775798
42	18.16947214595124	30.518369945887645	30.534689885560145	20.777468022600964
43	18.114375901592265	30.516412186258307	32.98954959905783	18.379662313091597
44	18.17013875555638	30.56212287129591	30.502636342329914	20.765102030817793
45	20.622152464051773	30.62725410962215	30.48213364656091	18.268459779765173
46	18.091482323497743	30.700447488576916	32.97423899216999	18.233831195755354
47	18.16597631652423	33.12593313950457	30.523544534080273	18.18454600989093
48	18.20032751236283	30.627041158225687	33.001687887207545	18.170943442203935
49	20.603808772868874	30.596350910782714	30.53633713376452	18.26350318258389
50	18.21435818566472	30.55761252369743	30.46467655136868	20.763352739269163
51	18.22177806966207	30.506947405216312	30.452710110348153	20.818564414773462
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.30494205071736
2	38.10727234092975
3	36.9275963165988
4	38.95317240242568
5	37.9012543449293
6	38.68052829574182
7	35.92889971264387
8	35.989717138565766
9	38.72880161041234
10	36.513044882763865
11	39.132894101884666
12	39.116427887247305
13	36.410292393634876
14	38.775684812490354
15	36.22082335706895
16	38.78000409090277
17	38.828558737883675
18	36.41211277917267
19	39.04372777959911
20	36.58342760723891
21	39.087245398465335
22	36.54339726806332
23	36.454047842379765
24	38.9587169278502
25	36.40847200809708
26	38.92824948840975
27	39.02144313718213
28	36.542169662896214
29	39.01564724039881
30	36.555248528141064
31	39.0610975124019
32	36.55885881308077
33	38.998619934449366
34	38.98807139684089
35	36.40324626658157
36	36.40476099429235
37	38.87100413776525
38	36.41067381727841
39	38.90592328612309
40	36.483977977374835
41	36.431723114501736
42	38.9469401685522
43	36.49403821468386
44	38.93524078637417
45	38.890612243816946
46	36.3253135192531
47	36.35052232641516
48	36.37127095456677
49	38.86731195545276
50	38.97771092493388
51	39.040342484435534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	64.5
2	74.0
3	133.0
4	192.0
5	366.0
6	540.0
7	1086.5
8	1633.0
9	3120.0
10	4607.0
11	7696.0
12	10785.0
13	16569.0
14	22353.0
15	31534.5
16	40716.0
17	55300.0
18	69884.0
19	89402.0
20	108920.0
21	132966.5
22	157013.0
23	184281.0
24	211549.0
25	238027.0
26	288225.5
27	311946.0
28	331764.5
29	351583.0
30	367294.5
31	383006.0
32	390716.5
33	398427.0
34	406037.5
35	413648.0
36	415858.5
37	418069.0
38	415286.5
39	412504.0
40	405379.0
41	398254.0
42	385956.0
43	373658.0
44	356073.0
45	338488.0
46	312648.5
47	286809.0
48	261120.5
49	235432.0
50	215713.5
51	195995.0
52	240645.0
53	285295.0
54	197331.5
55	109368.0
56	92846.0
57	76324.0
58	65752.5
59	55181.0
60	47118.0
61	39055.0
62	32386.0
63	25717.0
64	21053.5
65	16390.0
66	13352.0
67	10314.0
68	8297.5
69	6281.0
70	4989.0
71	3697.0
72	2897.5
73	2098.0
74	1614.0
75	853.5
76	577.0
77	443.0
78	309.0
79	229.5
80	150.0
81	110.5
82	71.0
83	51.0
84	31.0
85	25.5
86	20.0
87	15.0
88	10.0
89	8.5
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04390438937980458
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0014397594708039948
20	0.0
21	0.013785283208962386
22	4.964687830358603E-5
23	0.0
24	8.936438094645485E-4
25	0.0
26	0.0929720541031821
27	0.1157103243662245
28	0.12562315106750715
29	0.709884163903542
30	0.1401696864104579
31	0.03425634602947436
32	0.12504393748729867
33	0.015456728111849782
34	0.012047642468336875
35	0.017012330298695477
36	0.030400438481229176
37	0.04069389124950601
38	0.008092441163484523
39	0.016201431286403573
40	0.02674311844619834
41	0.012990933156105009
42	0.016333822961879803
43	0.021199217035631233
44	0.016085588570361874
45	0.02462485163857867
46	0.019544321092178366
47	0.00948255375598493
48	0.032899331355843005
49	0.012080740387205934
50	0.014596182221254294
51	0.011716663279646303
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6042676.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	12.093406327212824
#Duplication Level	Relative count
1	100.0
2	6.036991314315947
3	1.0234337110356686
4	0.6420572285172611
5	0.5004188527842688
6	0.39515453463251177
7	0.29705480358008907
8	0.23643137427803007
9	0.1631321370309951
10++	0.9297429566597593
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC	138092	2.285278906232934	TruSeq Adapter, Index 9 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3013165	3.70001	3.943509	5
AAAAA	3006825	3.5952463	3.9979515	3
TTTTC	1541620	3.0727687	3.2868805	4
GAAAA	1504890	3.001638	3.4497545	2
CGGAA	445240	2.4174929	40.705227	4
GGAAG	434680	2.4126217	41.486324	5
GAGCA	440430	2.391376	40.478355	9
CTCCA	456695	2.385679	38.340652	24
TCCAG	435710	2.326654	39.02992	25
GAAGA	671510	2.2342875	25.566467	6
CTGAA	681925	2.2314465	24.911064	19
TCTCG	390035	2.0938704	38.677826	41
TCGGA	378550	2.066361	40.50349	3
GCACA	365160	1.9395708	39.073177	11
CACAC	370845	1.9269316	38.128338	12
AGAGC	352700	1.9150339	39.96599	8
ATCGG	338125	1.8456963	40.07424	2
AGCAC	345445	1.8348533	39.019566	10
TCAGA	559515	1.8308873	23.845114	36
TCTGA	553840	1.8219903	24.683342	18
CCAGT	340460	1.8180274	38.418896	26
CACGA	333800	1.7730001	37.82265	31
GTCTG	318925	1.7501825	39.8547	17
GTCAC	327535	1.7490088	38.33936	29
CTCGT	325525	1.7475538	38.23951	42
CGTCT	322980	1.7338911	38.95899	16
CACGT	319525	1.7062362	38.785355	14
ACTCC	317270	1.6573522	37.70424	23
GATCG	301815	1.6474934	39.840305	1
ATGCC	307530	1.6421841	38.05601	47
ACACG	308755	1.6399722	38.596893	13
TGAAC	498810	1.6322438	24.335428	20
TCACG	305190	1.6296884	38.08363	30
ACGTC	299745	1.6006128	38.681957	15
CGATC	298985	1.5965544	37.63794	33
CAGTC	298070	1.5916684	38.18534	27
AAGAG	477980	1.590363	24.92211	7
AACTC	453815	1.4527142	23.580727	22
GAACT	440450	1.4412738	24.071922	21
ATCAG	435385	1.4246999	23.379322	35
CAGAT	428635	1.4026119	23.364056	37
GATCA	428205	1.4012047	23.389214	34
ATCTC	435285	1.4008349	23.292152	40
AGTCA	397595	1.3010404	23.731688	28
AGATC	388020	1.2697084	23.362928	38
GATCT	383465	1.2615006	23.498968	39
ACGAT	379260	1.2410432	23.451145	32
TCGTA	328425	1.0804332	23.507912	43
TATGC	318950	1.0492631	23.471275	46
GTATG	311505	1.0475513	23.982693	45
CGTAT	300075	0.9871691	23.417772	44
>>END_MODULE