FastQCFastQC Report
Wed 23 Jul 2014
LW189_ACTTGA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW189_ACTTGA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4268679
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC 180813 4.2358069088821155 TruSeq Adapter, Index 8 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1629970 4.1581173 4.6633434 1
AAAAA 1643230 4.0451837 4.2802978 2
CACAC 560270 3.3568013 57.171627 12
TTTTC 882365 3.0063028 3.5158784 2
GGAAG 410300 2.7046068 63.730003 5
TCCAG 423970 2.6410925 58.998367 25
CGGAA 410585 2.6216877 61.58487 4
GAGCA 405250 2.5876222 61.524914 9
GAAGA 538185 2.5547397 46.496895 6
TTGAA 725075 2.5142086 33.308796 36
CTGAA 530405 2.4563675 44.809 19
CTCCA 404995 2.4438436 57.08413 24
TCTCG 380185 2.3852775 58.654545 41
GATCG 367065 2.3605669 61.45989 1
ATCGG 359560 2.3123024 61.664577 2
TCGGA 350850 2.2562895 61.68874 3
AGAGC 345690 2.2073169 61.18916 8
CCAGT 353800 2.2039733 58.505035 26
GCACA 353570 2.1868975 59.202316 11
AGCAC 353410 2.1859083 59.227005 10
GTCTG 335900 2.1755984 61.310417 17
ATGCC 346695 2.159713 57.98769 47
CGTCT 336620 2.111951 59.336235 16
GTCAC 330990 2.06188 58.164856 29
TCTGA 441835 2.0608258 44.745148 18
CTCGT 325235 2.0405216 58.227028 42
ACGTC 321135 2.000489 58.979168 15
CACGT 319580 1.990802 59.131535 14
AAGAG 419020 1.9890689 45.84168 7
TGAAC 422355 1.9559753 44.252052 20
CAGTC 308335 1.9207523 58.095642 27
ACACG 310495 1.9204706 58.7377 13
ACTCC 313390 1.8910755 56.842754 23
CTTGA 398425 1.858351 43.39415 35
CACTT 407840 1.8426642 42.269974 33
TGAAT 524815 1.8198041 32.666103 37
TCACA 405605 1.8195511 42.094208 30
ATCTC 399830 1.806474 42.325783 40
GAACT 387790 1.7959007 44.08461 21
GAATC 379020 1.7552859 42.926514 38
AACTC 387705 1.7392515 42.64823 22
ACTTG 368900 1.7206392 43.35785 34
AGTCA 351230 1.6265872 43.2861 28
ACACT 347290 1.557949 41.72263 32
AATCT 451450 1.5163633 31.434357 39
TATGC 318085 1.4836257 43.179348 46
GTATG 305750 1.4722191 44.59882 45
TCGTA 308640 1.4395719 43.237053 43
CGTAT 299570 1.3972673 43.168514 44
Produced by FastQC (version 0.10.1)