##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW189_ACTTGA_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4268679	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.787604315058594	34.0	31.0	34.0	31.0	34.0
2	32.96327646093791	34.0	31.0	34.0	31.0	34.0
3	33.026579885721084	34.0	33.0	34.0	31.0	34.0
4	36.37451703442681	37.0	37.0	37.0	35.0	37.0
5	36.31899423685876	37.0	37.0	37.0	35.0	37.0
6	36.312981838175226	37.0	37.0	37.0	35.0	37.0
7	36.27145353398557	37.0	37.0	37.0	35.0	37.0
8	36.26887592156731	37.0	37.0	37.0	35.0	37.0
9	38.06016756003438	39.0	38.0	39.0	37.0	39.0
10	38.0031339906327	39.0	38.0	39.0	35.0	39.0
11	38.01645661339258	39.0	38.0	39.0	35.0	39.0
12	37.99910276692157	39.0	38.0	39.0	35.0	39.0
13	37.9813612126843	39.0	38.0	39.0	35.0	39.0
14	39.529737185672666	41.0	39.0	41.0	37.0	41.0
15	39.47509662825431	41.0	39.0	41.0	37.0	41.0
16	39.45149682138198	41.0	39.0	41.0	37.0	41.0
17	39.42508584037357	41.0	39.0	41.0	37.0	41.0
18	39.33744116153967	41.0	39.0	41.0	36.0	41.0
19	39.407658200581494	41.0	39.0	41.0	37.0	41.0
20	39.382322025151105	41.0	39.0	41.0	36.0	41.0
21	39.35141667949265	41.0	39.0	41.0	36.0	41.0
22	39.250801008930395	40.0	39.0	41.0	36.0	41.0
23	39.21639083191779	40.0	39.0	41.0	36.0	41.0
24	39.14404432846789	40.0	39.0	41.0	36.0	41.0
25	39.115181300819295	40.0	39.0	41.0	36.0	41.0
26	38.99005148899695	40.0	39.0	41.0	36.0	41.0
27	38.887996731541534	40.0	38.0	41.0	35.0	41.0
28	38.7175922574642	40.0	38.0	41.0	35.0	41.0
29	38.469180980813974	40.0	38.0	41.0	35.0	41.0
30	38.40548727135491	40.0	38.0	41.0	34.0	41.0
31	38.65891321413486	40.0	38.0	41.0	35.0	41.0
32	38.600094080627755	40.0	38.0	41.0	35.0	41.0
33	38.65799419445688	40.0	38.0	41.0	35.0	41.0
34	38.79908421317227	40.0	38.0	41.0	35.0	41.0
35	38.864369047192355	40.0	38.0	41.0	35.0	41.0
36	38.839598152027826	40.0	38.0	41.0	35.0	41.0
37	38.76276548318578	40.0	38.0	41.0	35.0	41.0
38	38.69016644259266	40.0	38.0	41.0	35.0	41.0
39	38.56538310798258	40.0	38.0	41.0	34.0	41.0
40	38.50232776931693	40.0	38.0	41.0	34.0	41.0
41	38.505570692947394	40.0	38.0	41.0	34.0	41.0
42	38.45026482431685	40.0	38.0	41.0	34.0	41.0
43	38.383729486335234	40.0	38.0	41.0	34.0	41.0
44	38.30079328991475	40.0	38.0	41.0	34.0	41.0
45	38.160343516108846	40.0	37.0	41.0	33.0	41.0
46	38.02144059087132	40.0	37.0	41.0	33.0	41.0
47	37.92412617580287	40.0	37.0	41.0	33.0	41.0
48	37.867862165320936	40.0	37.0	41.0	33.0	41.0
49	37.774431621585975	40.0	37.0	41.0	33.0	41.0
50	37.66536603009971	40.0	36.0	41.0	33.0	41.0
51	36.69870936652768	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	8.0
12	2.0
13	6.0
14	8.0
15	10.0
16	33.0
17	89.0
18	204.0
19	432.0
20	826.0
21	1397.0
22	2262.0
23	3604.0
24	5233.0
25	7626.0
26	11081.0
27	15380.0
28	20641.0
29	27385.0
30	35738.0
31	46082.0
32	58761.0
33	76902.0
34	104806.0
35	150620.0
36	239729.0
37	419012.0
38	863922.0
39	2175690.0
40	1185.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.56951109329902	25.34088472179743	29.56626390251162	22.523340282391928
2	19.401154314953175	32.063010594144	28.35905440535585	20.17678068554698
3	19.866708178338076	27.763718002688886	32.821863625725896	19.547710193247138
4	19.455363122877127	26.765376361164662	28.496942496730256	25.28231801922796
5	24.473590073181892	27.246110564884358	28.74582511357729	19.53447424835646
6	24.619044908272556	27.937120593982357	27.11393852758664	20.32989597015845
7	20.335096642310184	32.49698560140034	27.852597958291074	19.3153197979984
8	19.736808506800347	32.91976276501465	27.626462425495102	19.716966302689894
9	24.215805404903954	27.720847597113767	28.069948571911823	19.993398426070456
10	19.706869502251166	32.558690873687155	27.698053660160436	20.036385963901242
11	24.379720283488172	27.728250355672095	27.715670351413163	20.17635900942657
12	19.688128341344008	27.786230822228607	27.649912303080182	24.875728533347203
13	19.59479736002637	32.63644326500072	27.731881455597858	20.03687791937506
14	19.60353542629933	27.985402509769415	27.73954190511866	24.671520158812598
15	19.56689645672584	32.62702583164487	27.791736038245084	20.0143416733842
16	19.57664186039756	27.928897909634337	27.87801565777141	24.61644457219669
17	24.316937394449194	27.840861306272974	27.87824992228275	19.963951376995084
18	19.742079458305486	27.776321433398948	32.469576653573625	20.012022454721944
19	19.703407102824055	27.734917111229603	27.82375160825111	24.73792417769523
20	19.769769523545808	27.71604517463131	32.44682956952256	20.06735573230032
21	24.467109784158875	27.68475288612502	27.744779694350612	20.10335763536549
22	19.810536189424454	32.368616232004385	27.724180647966417	20.096666930604744
23	19.816997249031843	32.41714825593585	27.682990452081313	20.082864042950995
24	19.86518419571203	27.642815426650042	27.677065219148307	24.814935158489625
25	19.82458741919924	27.649724891471106	32.34731400510556	20.178373684224088
26	19.794567379704723	27.70936953954693	27.71335569959923	24.782707381149123
27	19.867607292035565	27.7277471193739	27.620941735481953	24.78370385310859
28	19.902719509717965	32.35487558821225	27.596378615649414	20.146026286420373
29	24.558647313498717	27.704484741131363	27.620259155285794	20.116608790084122
30	20.00485592334679	27.643270264976245	32.13894039999202	20.212933411684947
31	19.959949943287807	27.654013442195748	27.563320803532093	24.822715810984356
32	19.93155181662586	32.26343660919962	27.548529086490483	20.256482487684043
33	19.9371881431608	27.737592268387136	27.551110782046045	24.774108806406023
34	19.91490855211459	32.32943168809456	27.526209468631812	20.229450291159047
35	19.90999801776328	27.77659582943017	27.65349096254697	24.65991519025958
36	19.970520380891678	27.6658960878577	32.25318727877104	20.110396252479582
37	19.948524895140917	27.727888777775618	32.22507023220747	20.098516094875997
38	24.53937696433822	27.7520180774759	27.585089596665647	20.123515361520234
39	19.969418508831307	32.35942108852957	27.567133660001392	20.104026742637725
40	19.961134545100073	32.36241556324095	27.551684892220184	20.12476499943879
41	19.980159808664453	27.73650782642597	32.09907724835196	20.18425511655761
42	20.032858920561967	27.625064345985876	27.621245175954783	24.72083155749737
43	19.989718197057268	27.61809897242893	32.10401585478383	20.288166975729975
44	20.020801355954124	27.64005359898838	27.590358086509553	24.748786958547946
45	24.559563881250664	27.697266290893456	27.57558325493334	20.167586572922534
46	19.984662059192363	27.803731529332815	32.1169591794272	20.094647232047627
47	19.98682839023108	32.33032766663598	27.587634430897197	20.09520951223575
48	20.07081324149968	27.722969263097507	32.09055133702233	20.115666158380478
49	24.624233008453103	27.62348068958424	27.548108226141753	20.204178075820902
50	20.13774049174021	27.60786836818707	27.467639606694057	24.786751533378663
51	20.13191971701433	27.531944364395123	27.455870176716097	24.880265741874446
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.09285137569095
2	39.577935000500155
3	39.414418371585214
4	44.73768114210508
5	44.00806432153835
6	44.94894087843101
7	39.65041644030858
8	39.45377480949024
9	44.20920383097441
10	39.74325546615241
11	44.55607929291474
12	44.56385687469121
13	39.63167527940142
14	44.27505558511193
15	39.581238130110044
16	44.19308643259426
17	44.28088877144428
18	39.75410191302743
19	44.441331280519286
20	39.83712525584613
21	44.57046741952436
22	39.9072031200292
23	39.89986129198284
24	44.68011935420165
25	40.00296110342333
26	44.57727476085385
27	44.651311145144156
28	40.048745796138334
29	44.675256103582846
30	40.21778933503173
31	44.78266575427216
32	40.1880343043099
33	44.71129694956682
34	40.14435884327363
35	44.569913208022854
36	40.08091663337125
37	40.04704099001691
38	44.66289232585845
39	40.07344525146904
40	40.08589954453886
41	40.16441492522206
42	44.75369047805934
43	40.277885172787244
44	44.769588314502066
45	44.727150454173206
46	40.07930929123999
47	40.08203790246683
48	40.186479399880156
49	44.82841108427401
50	44.92449202511887
51	45.01218545888877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	27.5
2	23.0
3	53.0
4	83.0
5	201.0
6	319.0
7	640.0
8	961.0
9	1710.5
10	2460.0
11	4011.0
12	5562.0
13	8549.0
14	11536.0
15	16217.5
16	20899.0
17	27895.0
18	34891.0
19	44961.0
20	55031.0
21	67205.5
22	79380.0
23	92828.0
24	106276.0
25	119542.0
26	146228.0
27	159648.0
28	170191.5
29	180735.0
30	190451.0
31	200167.0
32	206737.0
33	213307.0
34	220202.5
35	227098.0
36	231620.0
37	236142.0
38	238194.5
39	240247.0
40	239919.0
41	239591.0
42	238414.0
43	237237.0
44	232692.5
45	228148.0
46	220135.5
47	212123.0
48	207651.5
49	203180.0
50	283526.0
51	363872.0
52	262015.0
53	160158.0
54	147479.0
55	134800.0
56	124887.5
57	114975.0
58	106805.0
59	98635.0
60	90930.5
61	83226.0
62	76090.5
63	68955.0
64	62672.0
65	56389.0
66	51151.0
67	45913.0
68	41095.5
69	36278.0
70	31744.5
71	27211.0
72	23501.0
73	19791.0
74	16580.5
75	10778.0
76	8186.0
77	6412.0
78	4638.0
79	3557.5
80	2477.0
81	1793.0
82	1109.0
83	794.0
84	479.0
85	333.5
86	188.0
87	133.0
88	78.0
89	61.0
90	44.0
91	27.5
92	11.0
93	10.0
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044838227470371986
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0015695722259743588
20	0.0
21	0.013727900364492153
22	2.3426451133945653E-5
23	0.0
24	9.370580453578261E-4
25	0.0
26	0.0919019677984688
27	0.11359486154850248
28	0.12502696970186797
29	0.7042459739886742
30	0.1369041804267784
31	0.03558477927246345
32	0.12434760261898353
33	0.01628138353809223
34	0.013235944890679295
35	0.01810864672653999
36	0.031016621301344045
37	0.041277406898012244
38	0.008995757235435132
39	0.01728872093685189
40	0.02661244848816226
41	0.013470209402018752
42	0.017241868034584003
43	0.022137996321578642
44	0.01611739838015461
45	0.024222950472499807
46	0.01946738089230884
47	0.009557992062649828
48	0.03275017868525602
49	0.012697136514598544
50	0.015297472590466514
51	0.011174417190892077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4268679.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	22.659653471200645
#Duplication Level	Relative count
1	100.0
2	16.772473181412302
3	2.776656514134038
4	0.8837596173840715
5	0.48744388585128373
6	0.29907159370298336
7	0.22078700475823518
8	0.1645199564541974
9	0.09602094112754272
10++	0.6189375313444155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC	180813	4.2358069088821155	TruSeq Adapter, Index 8 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1629970	4.1581173	4.6633434	1
AAAAA	1643230	4.0451837	4.2802978	2
CACAC	560270	3.3568013	57.171627	12
TTTTC	882365	3.0063028	3.5158784	2
GGAAG	410300	2.7046068	63.730003	5
TCCAG	423970	2.6410925	58.998367	25
CGGAA	410585	2.6216877	61.58487	4
GAGCA	405250	2.5876222	61.524914	9
GAAGA	538185	2.5547397	46.496895	6
TTGAA	725075	2.5142086	33.308796	36
CTGAA	530405	2.4563675	44.809	19
CTCCA	404995	2.4438436	57.08413	24
TCTCG	380185	2.3852775	58.654545	41
GATCG	367065	2.3605669	61.45989	1
ATCGG	359560	2.3123024	61.664577	2
TCGGA	350850	2.2562895	61.68874	3
AGAGC	345690	2.2073169	61.18916	8
CCAGT	353800	2.2039733	58.505035	26
GCACA	353570	2.1868975	59.202316	11
AGCAC	353410	2.1859083	59.227005	10
GTCTG	335900	2.1755984	61.310417	17
ATGCC	346695	2.159713	57.98769	47
CGTCT	336620	2.111951	59.336235	16
GTCAC	330990	2.06188	58.164856	29
TCTGA	441835	2.0608258	44.745148	18
CTCGT	325235	2.0405216	58.227028	42
ACGTC	321135	2.000489	58.979168	15
CACGT	319580	1.990802	59.131535	14
AAGAG	419020	1.9890689	45.84168	7
TGAAC	422355	1.9559753	44.252052	20
CAGTC	308335	1.9207523	58.095642	27
ACACG	310495	1.9204706	58.7377	13
ACTCC	313390	1.8910755	56.842754	23
CTTGA	398425	1.858351	43.39415	35
CACTT	407840	1.8426642	42.269974	33
TGAAT	524815	1.8198041	32.666103	37
TCACA	405605	1.8195511	42.094208	30
ATCTC	399830	1.806474	42.325783	40
GAACT	387790	1.7959007	44.08461	21
GAATC	379020	1.7552859	42.926514	38
AACTC	387705	1.7392515	42.64823	22
ACTTG	368900	1.7206392	43.35785	34
AGTCA	351230	1.6265872	43.2861	28
ACACT	347290	1.557949	41.72263	32
AATCT	451450	1.5163633	31.434357	39
TATGC	318085	1.4836257	43.179348	46
GTATG	305750	1.4722191	44.59882	45
TCGTA	308640	1.4395719	43.237053	43
CGTAT	299570	1.3972673	43.168514	44
>>END_MODULE