##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename LW188_CAGATC_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6425498 Filtered Sequences 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80803137749012 34.0 31.0 34.0 31.0 34.0 2 32.99719741567113 34.0 33.0 34.0 31.0 34.0 3 33.05739430624677 34.0 33.0 34.0 31.0 34.0 4 36.39008540660973 37.0 37.0 37.0 35.0 37.0 5 36.33155437913139 37.0 37.0 37.0 35.0 37.0 6 36.331277046541764 37.0 37.0 37.0 35.0 37.0 7 36.317898628246404 37.0 37.0 37.0 35.0 37.0 8 36.304651250377795 37.0 37.0 37.0 35.0 37.0 9 38.09806617323669 39.0 38.0 39.0 37.0 39.0 10 38.06399363909225 39.0 38.0 39.0 37.0 39.0 11 38.06023424176616 39.0 38.0 39.0 37.0 39.0 12 38.04630940667945 39.0 38.0 39.0 37.0 39.0 13 38.027114629869935 39.0 38.0 39.0 35.0 39.0 14 39.56467358638972 41.0 40.0 41.0 37.0 41.0 15 39.52171660468963 41.0 39.0 41.0 37.0 41.0 16 39.48847995906309 41.0 39.0 41.0 37.0 41.0 17 39.468417856483654 41.0 39.0 41.0 37.0 41.0 18 39.41704362836935 41.0 39.0 41.0 37.0 41.0 19 39.467327201720394 41.0 39.0 41.0 37.0 41.0 20 39.44574303812716 41.0 39.0 41.0 37.0 41.0 21 39.41457704912522 41.0 39.0 41.0 37.0 41.0 22 39.34491770132058 40.0 39.0 41.0 36.0 41.0 23 39.283630778501525 40.0 39.0 41.0 36.0 41.0 24 39.214112898330995 40.0 39.0 41.0 36.0 41.0 25 39.19877525446277 40.0 39.0 41.0 36.0 41.0 26 39.074954345951085 40.0 39.0 41.0 36.0 41.0 27 38.984037813100244 40.0 39.0 41.0 36.0 41.0 28 38.8697828557413 40.0 38.0 41.0 35.0 41.0 29 38.5966981858838 40.0 38.0 41.0 35.0 41.0 30 38.59539649689409 40.0 38.0 41.0 35.0 41.0 31 38.83087396494404 40.0 38.0 41.0 35.0 41.0 32 38.81946270934953 40.0 38.0 41.0 35.0 41.0 33 38.83512266286598 40.0 38.0 41.0 35.0 41.0 34 38.976059287544714 40.0 39.0 41.0 36.0 41.0 35 39.03497612169516 40.0 39.0 41.0 36.0 41.0 36 39.02665614400627 40.0 39.0 41.0 35.0 41.0 37 38.95814799101953 40.0 39.0 41.0 35.0 41.0 38 38.92158989077578 40.0 39.0 41.0 35.0 41.0 39 38.881730723439645 40.0 39.0 41.0 35.0 41.0 40 38.8289832165538 40.0 38.0 41.0 35.0 41.0 41 38.80882929229765 40.0 38.0 41.0 35.0 41.0 42 38.75370189205568 40.0 38.0 41.0 35.0 41.0 43 38.69716588504113 40.0 38.0 41.0 35.0 41.0 44 38.62488603996142 40.0 38.0 41.0 35.0 41.0 45 38.53435873764182 40.0 38.0 41.0 34.0 41.0 46 38.47363037075103 40.0 38.0 41.0 34.0 41.0 47 38.38838919567012 40.0 38.0 41.0 34.0 41.0 48 38.3155982618001 40.0 38.0 41.0 34.0 41.0 49 38.25275394996621 40.0 38.0 41.0 34.0 41.0 50 38.14081787901887 40.0 37.0 41.0 34.0 41.0 51 37.16749067543092 39.0 36.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 1.0 12 6.0 13 6.0 14 4.0 15 21.0 16 28.0 17 78.0 18 218.0 19 459.0 20 839.0 21 1520.0 22 2510.0 23 4247.0 24 6316.0 25 9375.0 26 13577.0 27 19408.0 28 26218.0 29 35577.0 30 47209.0 31 61621.0 32 80010.0 33 105145.0 34 144178.0 35 205419.0 36 316614.0 37 553545.0 38 1257168.0 39 3532271.0 40 1908.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.827689151065975 26.546301663601724 32.44467882398973 22.18133036134257 2 19.08057554449476 30.467397235202625 29.928341740982567 20.523685479320047 3 19.93700721718379 30.039601599751492 30.666136694774476 19.357254488290245 4 19.379307253694577 28.880407401885428 30.31414841308798 21.426136931332014 5 20.90367159090237 29.422777814264357 31.01485674729025 18.658693847543024 6 20.88370426696888 30.052907961375137 29.438465314283814 19.624922457372175 7 19.86210251719011 31.233999294685017 30.21084124530114 18.693056942823734 8 19.271191120127966 31.69801002194694 29.855071155574247 19.175727702350855 9 20.507640030391418 29.647165091328333 30.235493031045994 19.609701847234255 10 19.24355123914131 31.059164597047573 29.901853521703686 19.79543064210743 11 20.6733392493469 29.520544555457022 29.912265166061836 19.89385102913424 12 19.25324698568111 29.55109471670523 29.851802926403526 21.34385537121014 13 19.150904723649436 31.181458620016688 29.86573180786921 19.801904848464662 14 19.14877259319044 29.84593567689228 29.86973149785433 21.135560232062947 15 19.079003681893607 31.275552494141312 29.95388061750233 19.691563206462753 16 19.07028840410502 29.81214841246546 29.992492410704973 21.12507077272454 17 20.5423610745813 29.73204567179073 30.046184747081085 19.679408506546885 18 19.138002999923117 29.689216306658256 31.49153575333772 19.68124494008091 19 19.15987280496429 29.627332614520352 30.02291838899569 21.189876191519666 20 19.198371861605125 29.67363774761116 31.419292325668764 19.70869806511495 21 20.63910865167721 29.611646103012728 30.001413316680686 19.747831928629374 22 19.24713971452783 31.038791563918423 30.014605878613242 19.699462842940505 23 19.2404697659232 31.13497195081222 29.97892147814846 19.64563680511612 24 19.261623824357738 29.674866592461445 29.996168346244545 21.067341236936272 25 19.17804658876246 29.696032898928614 31.450558384735317 19.675362127573614 26 19.204135109142744 29.78951854571465 29.964369373424983 21.041976971717624 27 19.277721515042927 29.764875434305033 29.9040088496954 21.05339420095664 28 19.22826712099626 31.20116503504937 29.92297349454887 19.64759434940549 29 20.723790475847135 29.78356192940782 29.886761281747244 19.605886312997807 30 19.374083256087367 29.79326548155666 31.230056047221545 19.602595215134432 31 19.280411165152156 29.74117537897535 29.88851381383453 21.08989964203797 32 19.294071267575568 31.188231820239352 29.86626714316544 19.651429769019632 33 19.333436169297052 29.771291675221196 29.891021377479955 21.004250778001797 34 19.29930622020368 29.836749363118148 29.871054675102705 20.99288974157547 35 19.300412240061814 31.294739503008213 29.880041940025016 19.524806316904954 36 20.731609735077562 29.87969704291166 29.87726850421943 19.51142471779135 37 19.297399968861903 31.279931496185583 29.900747314339092 19.521921220613418 38 19.34610663845171 29.89370209504741 31.29031735907543 19.469873907425445 39 19.341001373821797 29.87201634265787 29.872950279978845 20.91403200354149 40 19.321978964731528 31.250728737256534 29.85278300963778 19.574509288374156 41 19.335137421929794 29.85451695076444 31.253033231610793 19.557312395694968 42 19.370206624440936 29.798309901949782 29.877399479886012 20.954083993723266 43 19.314786060358415 29.815313369525033 31.268974373941976 19.600926196174576 44 19.38413548832471 29.858735318816432 29.84464854271912 20.912480650139738 45 20.76860869761494 29.86658509685248 29.87123955762386 19.49356664790872 46 19.320997310980616 29.923983640177298 31.26771503399216 19.487304014849922 47 19.370325884736506 31.313990504723904 29.842460846344345 19.473222764195246 48 19.41876771631969 29.89708343919535 31.249657505243285 19.43449133924168 49 20.765709031004036 29.863533182499076 29.86202338719619 19.5087343993007 50 19.424433556362946 29.860809101575548 29.800384711290643 20.914372630770863 51 19.420392221196856 29.815528687072096 29.826315026803197 20.93776406492785 >>END_MODULE >>Per base GC content pass #Base %GC 1 41.00901951240854 2 39.60426102381481 3 39.294261705474035 4 40.80544418502659 5 39.5623654384454 6 40.508626724341056 7 38.55515946001384 8 38.44691882247882 9 40.11734187762567 10 39.03898188124874 11 40.56719027848114 12 40.59710235689125 13 38.9528095721141 14 40.28433282525339 15 38.770566888356356 16 40.19535917682956 17 40.221769581128186 18 38.819247940004026 19 40.34974899648396 20 38.90706992672008 21 40.386940580306586 22 38.94660255746834 23 38.88610657103932 24 40.32896506129401 25 38.85340871633608 26 40.24611208086037 27 40.331115715999566 28 38.87586147040176 29 40.32967678884494 30 38.976678471221796 31 40.37031080719013 32 38.945501036595196 33 40.33768694729885 34 40.29219596177915 35 38.82521855696677 36 40.24303445286891 37 38.819321189475325 38 38.81598054587716 39 40.25503337736329 40 38.89648825310569 41 38.89244981762476 42 40.324290618164206 43 38.91571225653299 44 40.296616138464444 45 40.26217534552366 46 38.80830132583054 47 38.84354864893175 48 38.85325905556137 49 40.274443430304736 50 40.338806187133805 51 40.358156286124704 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15.0 1 20.5 2 26.0 3 75.0 4 124.0 5 257.0 6 390.0 7 833.5 8 1277.0 9 2477.5 10 3678.0 11 6419.0 12 9160.0 13 14216.0 14 19272.0 15 26958.5 16 34645.0 17 47115.0 18 59585.0 19 76431.5 20 93278.0 21 114777.5 22 136277.0 23 159951.5 24 183626.0 25 207500.0 26 254240.0 27 277106.0 28 297803.5 29 318501.0 30 336696.0 31 354891.0 32 369694.5 33 384498.0 34 396110.0 35 407722.0 36 418923.0 37 430124.0 38 431743.5 39 433363.0 40 434242.0 41 435121.0 42 427364.5 43 419608.0 44 404357.0 45 389106.0 46 367836.5 47 346567.0 48 323725.5 49 300884.0 50 279526.5 51 258169.0 52 272288.5 53 286408.0 54 222798.0 55 159188.0 56 139385.5 57 119583.0 58 107700.0 59 95817.0 60 84304.0 61 72791.0 62 62417.0 63 52043.0 64 43691.0 65 35339.0 66 30196.5 67 25054.0 68 21398.0 69 17742.0 70 15083.0 71 12424.0 72 10413.0 73 8402.0 74 6846.5 75 4279.5 76 3268.0 77 2582.5 78 1897.0 79 1453.0 80 1009.0 81 744.5 82 480.0 83 342.5 84 205.0 85 149.0 86 93.0 87 67.5 88 42.0 89 33.5 90 25.0 91 16.5 92 8.0 93 5.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04461910967834711 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0014006696445940845 20 0.0 21 0.013913318469634572 22 4.6688988153136144E-5 23 0.0 24 0.0011516617077773584 25 0.0 26 0.08933159733300049 27 0.11202244557542466 28 0.12196720005204266 29 0.709874938876333 30 0.1364252233834638 31 0.03409852434784043 32 0.12221620798885939 33 0.015983196944423607 34 0.013026227694724985 35 0.016901413711435286 36 0.02928955856806741 37 0.03887636413551136 38 0.008341765883360325 39 0.016870287719333195 40 0.026690538227542828 41 0.012948412714469757 42 0.0176951265100386 43 0.02136799357808531 44 0.016076574920729877 45 0.024169332867273477 46 0.0190335441704285 47 0.009633494555597092 48 0.031779637936234666 49 0.012232514896121672 50 0.014629216287982659 51 0.010971912215986994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 6425498.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 19.94625059211353 #Duplication Level Relative count 1 100.0 2 12.259388962685666 3 2.8957855331481706 4 1.359739161936964 5 0.848931288491728 6 0.5023547880690737 7 0.33510445598357685 8 0.25661152034778406 9 0.19864750633981404 10++ 2.1017992996014976 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC 80979 1.260275857217604 TruSeq Adapter, Index 7 (100% over 51bp) >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position AAAAA 2786050 3.7494059 3.940054 3 TTTTT 2855715 3.7298975 4.0271597 1 TTTTC 1557080 3.063837 3.4103289 1 GAAAA 1477500 3.0565476 3.2512226 2 GAAGA 695135 2.2105634 15.839108 6 CTCCA 474755 2.132888 20.731007 24 TCCAG 437760 2.018786 21.161692 25 GAGCA 423550 2.0170324 22.233793 9 GGAAG 409370 2.001151 23.129204 5 CGGAA 418070 1.9909354 22.502092 4 CACCA 436820 1.9742372 20.374052 31 CTGAA 631645 1.945149 14.9916 19 CCAGA 388785 1.8036906 20.641766 33 TCTCG 385480 1.7670863 20.691963 41 TCGGA 356780 1.688925 22.017828 3 TCATC 540300 1.6112418 13.688668 38 CACAC 356450 1.6109996 20.738121 12 TCACC 355220 1.5958642 19.905846 30 AGAGC 326415 1.5544554 21.798937 8 TCTGA 502020 1.5367473 14.454426 18 ATCGG 317650 1.5036914 21.657793 2 GCACA 323470 1.5006747 21.230894 11 AAGAG 468195 1.4888829 15.06574 7 GATCG 307000 1.4532765 21.379467 1 AGCAC 308645 1.4318969 21.140501 10 CTCGT 312275 1.4315057 20.313204 42 CGTCT 308390 1.4136964 20.715092 16 CCAGT 305605 1.4093364 20.514053 26 TGAAC 451725 1.3910859 14.40585 20 ACCAG 293155 1.3600342 20.262043 32 CACGT 293030 1.3513452 20.81521 14 GTCTG 287100 1.350967 21.205126 17 CATCT 441220 1.315773 13.454437 39 ATCTC 434825 1.2967024 13.579215 40 GTCAC 280970 1.295729 20.31451 29 ACGTC 276415 1.274723 20.654282 15 ACACG 273165 1.2672944 20.953184 13 ACTCC 280575 1.2605134 19.97256 23 AACTC 418460 1.2553881 13.804623 22 GATCA 404860 1.246765 13.768049 36 GAACT 403010 1.241068 14.220758 21 ATGCC 267150 1.2319963 20.149046 47 CAGAT 396790 1.2219136 13.699462 34 CAGTC 259835 1.1982622 20.311241 27 AGATC 382770 1.1787391 13.673578 35 ATCAT 529205 1.0538416 9.192067 37 AGTCA 329475 1.0146172 13.738659 28 TCGTA 284555 0.8710592 13.447335 43 GTATG 260090 0.8172611 13.744141 45 TATGC 265380 0.8123621 13.36446 46 CGTAT 242425 0.74209386 13.323209 44 >>END_MODULE