FastQCFastQC Report
Wed 23 Jul 2014
LW187_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW187_GCCAAT_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5486215
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC 196508 3.581850146230142 TruSeq Adapter, Index 6 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1969210 4.324124 4.663838 2
AAAAA 1946790 4.1334367 4.292755 24
CGCCA 528315 3.1442087 61.943253 33
ATTTT 1388275 3.0280204 3.2255862 3
ACGCC 426640 2.5391011 61.5065 32
TCCAG 524930 2.4477513 49.24925 25
GGAAG 495370 2.4261787 53.094627 5
CACGC 398990 2.374545 61.732967 31
CGGAA 495180 2.3584745 51.62496 4
GAAGA 636215 2.3582869 40.690136 6
CTGAA 649565 2.3572907 39.27357 19
GAGCA 483810 2.3043208 51.4785 9
GCCAA 481485 2.2301078 47.946358 34
GATCG 461545 2.2131195 51.433792 1
CTCCA 468325 2.1236758 47.448715 24
ATCGG 439480 2.1073174 51.52446 2
CCAGT 443905 2.0699313 48.743286 26
TCTCG 437050 2.051728 48.94464 41
ATGCC 436105 2.0335598 48.718445 47
AGCAC 425135 1.9691098 49.651264 10
GTCTG 407000 1.9647534 51.08636 17
AGAGC 407925 1.9428909 51.072655 8
GCACA 418185 1.9369193 49.622997 11
TCGGA 400385 1.9198558 51.4648 3
TCTGA 523335 1.9120232 39.10732 18
TCACG 400465 1.8673702 48.65694 30
TGAAC 513295 1.8627628 38.681454 20
GTCAC 396355 1.8482053 48.770798 29
CACAC 406475 1.8308467 47.904663 12
CGTCT 388250 1.8226367 49.500305 16
AAGAG 483190 1.7910624 40.054085 7
ACGTC 381775 1.7802187 49.26899 15
CTCGT 374875 1.7598479 48.53626 42
CACGT 373185 1.7401636 49.280533 14
CAGTC 370215 1.7263144 48.572384 27
CCAAT 486395 1.716543 36.67847 35
GAACT 465915 1.6908194 38.49316 21
ATCTC 473110 1.6809332 37.11656 40
ACTCC 364005 1.6506244 47.12669 23
ACACG 356235 1.6499835 49.04458 13
AATAT 758240 1.6317153 23.169407 37
AACTC 454480 1.6039112 37.18652 22
AGTCA 413875 1.5019647 38.035286 28
CAATA 525585 1.4435545 28.708775 36
TATGC 382935 1.3990667 37.922802 46
ATATC 494545 1.3674732 28.90554 38
GTATG 363500 1.3656604 38.95145 45
TCGTA 362875 1.3257766 37.862198 43
CGTAT 354765 1.2961464 37.79142 44
TATCT 440405 1.2259933 28.975912 39
Produced by FastQC (version 0.10.1)