##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW187_GCCAAT_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5486215	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.759832598613066	34.0	31.0	34.0	31.0	34.0
2	32.93536983147762	34.0	31.0	34.0	31.0	34.0
3	32.997072116203974	34.0	31.0	34.0	31.0	34.0
4	36.354744755719565	37.0	37.0	37.0	35.0	37.0
5	36.2969355010695	37.0	37.0	37.0	35.0	37.0
6	36.28851804021534	37.0	37.0	37.0	35.0	37.0
7	36.26352685776988	37.0	37.0	37.0	35.0	37.0
8	36.261576332681095	37.0	37.0	37.0	35.0	37.0
9	38.03762411790278	39.0	38.0	39.0	35.0	39.0
10	37.98836082800255	39.0	38.0	39.0	35.0	39.0
11	37.99267145017102	39.0	38.0	39.0	35.0	39.0
12	37.97882365164325	39.0	38.0	39.0	35.0	39.0
13	37.96064572751888	39.0	38.0	39.0	35.0	39.0
14	39.49907085303802	41.0	39.0	41.0	37.0	41.0
15	39.456677144442935	41.0	39.0	41.0	37.0	41.0
16	39.42299691134963	41.0	39.0	41.0	37.0	41.0
17	39.392873775453566	41.0	39.0	41.0	36.0	41.0
18	39.28504606545678	40.0	39.0	41.0	36.0	41.0
19	39.35758988665227	40.0	39.0	41.0	36.0	41.0
20	39.344540817303006	40.0	39.0	41.0	36.0	41.0
21	39.29933259998013	40.0	39.0	41.0	36.0	41.0
22	39.2403885009975	40.0	39.0	41.0	36.0	41.0
23	39.18136420100197	40.0	39.0	41.0	36.0	41.0
24	39.089917001065395	40.0	39.0	41.0	36.0	41.0
25	39.05998361347486	40.0	39.0	41.0	36.0	41.0
26	38.91184632756828	40.0	38.0	41.0	35.0	41.0
27	38.81864236089909	40.0	38.0	41.0	35.0	41.0
28	38.68173467499906	40.0	38.0	41.0	35.0	41.0
29	38.40182056299288	40.0	38.0	41.0	34.0	41.0
30	38.36420009059069	40.0	38.0	41.0	34.0	41.0
31	38.59232148211472	40.0	38.0	41.0	35.0	41.0
32	38.5459169937744	40.0	38.0	41.0	34.0	41.0
33	38.56958558860708	40.0	38.0	41.0	34.0	41.0
34	38.70403839441218	40.0	38.0	41.0	35.0	41.0
35	38.77288130341228	40.0	38.0	41.0	35.0	41.0
36	38.73914492960994	40.0	38.0	41.0	35.0	41.0
37	38.62482640581895	40.0	38.0	41.0	35.0	41.0
38	38.54419649977261	40.0	38.0	41.0	34.0	41.0
39	38.52089227272354	40.0	38.0	41.0	34.0	41.0
40	38.428297286927325	40.0	38.0	41.0	34.0	41.0
41	38.40236319575518	40.0	38.0	41.0	34.0	41.0
42	38.31760220844426	40.0	38.0	41.0	34.0	41.0
43	38.238094934303525	40.0	38.0	41.0	34.0	41.0
44	38.13652691336377	40.0	37.0	41.0	33.0	41.0
45	38.0039608363872	40.0	37.0	41.0	33.0	41.0
46	37.87657027659324	40.0	37.0	41.0	33.0	41.0
47	37.757963550462385	40.0	37.0	41.0	33.0	41.0
48	37.68239870293089	40.0	36.0	41.0	33.0	41.0
49	37.58582228366916	40.0	36.0	41.0	33.0	41.0
50	37.4573501038512	40.0	36.0	41.0	33.0	41.0
51	36.485197536006154	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	3.0
12	3.0
13	3.0
14	7.0
15	11.0
16	41.0
17	120.0
18	287.0
19	634.0
20	1170.0
21	1960.0
22	3146.0
23	4929.0
24	7419.0
25	10746.0
26	14992.0
27	20264.0
28	27641.0
29	36772.0
30	47512.0
31	61362.0
32	78596.0
33	103410.0
34	139643.0
35	202801.0
36	326958.0
37	569070.0
38	1147816.0
39	2677573.0
40	1322.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.89428120596714	24.5394141431419	27.86081482087978	23.705489830011174
2	20.675292528637684	30.642054677040548	27.137124593184918	21.54552820113685
3	21.078539576010055	27.014690455988326	31.556127494092014	20.35064247390961
4	20.347744300943365	26.29654506795669	28.054624180787663	25.301086450312283
5	24.566153532079948	26.905926945991	28.40738104503743	20.120538476891628
6	24.70736199729686	27.449963226012837	27.003790409234785	20.838884367455524
7	21.014797998255627	31.405404272344413	27.652944698667483	19.92685303073248
8	20.474881133896503	31.707142355886525	27.43144408303357	20.386532427183404
9	24.420351736124086	27.192846069649114	27.72592762040861	20.660874573818198
10	20.554553549213804	31.351305043641197	27.310176506024646	20.783964901120353
11	24.555326395338135	27.288576915049813	27.32131351031631	20.834783179295744
12	20.54576789280041	27.349420319837993	27.205295454151905	24.89951633320969
13	20.406929002964702	31.522351931158365	27.276328033079274	20.79439103279766
14	20.411485878697793	27.551326369819627	27.330518399297148	24.706669352185433
15	20.36956262195339	31.53937641889718	27.417919275857766	20.673141683291668
16	20.37656198307941	27.426504429738902	27.459332162520063	24.73760142466163
17	24.49448299054995	27.360156319065148	27.467498083833753	20.677862606551148
18	20.523275154181892	27.314842746775327	31.44131245312114	20.720569645921643
19	20.526596497060957	27.249069290298305	27.405773389504017	24.81856082313672
20	20.54050014445296	27.321131235286988	31.367254108707005	20.771114511553044
21	24.60562233592041	27.243241154189295	27.33462790298311	20.816508606907185
22	20.567994716938625	31.326198964568093	27.32504188226013	20.780764436233152
23	20.507253179104353	31.42498061049376	27.32532356096143	20.742442649440463
24	20.531679620776714	27.360261574532718	27.37161742063348	24.736441384057084
25	20.549941990971917	27.298529131650874	31.345125920147133	20.806402957230073
26	20.50442849547919	27.358425161108347	27.374005992234395	24.763140351178066
27	20.539597444277188	27.353307163932723	27.344675677840826	24.762419713949267
28	20.609003114969155	31.30929081343255	27.281348072799904	20.800357998798397
29	24.63123007377693	27.37534712859558	27.269067820332538	20.724354977294958
30	20.684093698053342	27.323573256340104	31.216043874166594	20.776289171439956
31	20.63040269102515	27.326658040837998	27.22737470424688	24.815564563889968
32	20.58679139019306	31.32287944029174	27.270830922801036	20.81949824671416
33	20.650215721312783	27.378904924017167	27.25738052014487	24.713498834525183
34	24.58869389243996	27.303973210892035	27.296918264526948	20.810414632141057
35	20.622743024158027	27.38710745392707	27.28244430119069	24.707705220724215
36	20.567491880327154	27.417062462542297	27.335961458060403	24.679484199070146
37	20.618344647599567	31.3716690870711	27.278748168285095	20.731238097044237
38	20.612206021617887	31.39380593484738	27.307316157269717	20.686671886265014
39	20.622832613894534	27.44514696933291	31.243193163392885	20.688827253379674
40	20.622423085941076	31.341674509613732	27.31783243672738	20.71806996771781
41	20.649268211937432	27.373799282030266	31.229185914492618	20.74774659153968
42	20.661209607583974	27.33966546647828	27.309037874299257	24.690087051638486
43	20.629928408856387	27.29673971146224	31.233304576305425	20.840027303375948
44	20.665854441554522	27.3382904223127	27.32634950995772	24.669505626175063
45	24.577814574064245	27.34623881442219	27.360459797190558	20.715486814323008
46	20.648439928425123	27.39861061608903	31.24784760349051	20.705101851995337
47	20.622870258513906	31.384357258990235	27.36700377946228	20.62576870303358
48	20.708528743399242	27.429109473458112	31.206236152787348	20.656125630355294
49	24.622007598130363	27.349823901297228	27.25553999956249	20.772628501009923
50	20.72847724374546	27.35720086812028	27.199473512942955	24.714848375191302
51	20.70844234633906	27.288161114659808	27.228313339108475	24.775083199892663
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.599771035978314
2	42.22082072977453
3	41.42918204991966
4	45.648830751255645
5	44.686692008971576
6	45.546246364752385
7	40.941651028988105
8	40.86141356107991
9	45.08122630994229
10	41.338518450334156
11	45.39010957463388
12	45.4452842260101
13	41.201320035762365
14	45.118155230883225
15	41.04270430524505
16	45.11416340774104
17	45.1723455971011
18	41.24384480010353
19	45.34515732019768
20	41.31161465600601
21	45.422130942827586
22	41.34875915317178
23	41.249695828544816
24	45.2681210048338
25	41.35634494820199
26	45.267568846657255
27	45.302017158226455
28	41.40936111376755
29	45.35558505107189
30	41.4603828694933
31	45.44596725491512
32	41.406289636907225
33	45.363714555837966
34	45.39910852458102
35	45.33044824488224
36	45.2469760793973
37	41.34958274464381
38	41.298877907882904
39	41.31165986727421
40	41.34049305365888
41	41.39701480347712
42	45.35129665922246
43	41.469955712232334
44	45.33536006772959
45	45.29330138838725
46	41.353541780420464
47	41.248638961547485
48	41.36465437375454
49	45.39463609914029
50	45.44332561893676
51	45.483525546231725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15.0
1	21.5
2	28.0
3	77.5
4	127.0
5	228.5
6	330.0
7	710.0
8	1090.0
9	1987.0
10	2884.0
11	4854.5
12	6825.0
13	10276.5
14	13728.0
15	19443.5
16	25159.0
17	33614.0
18	42069.0
19	54381.0
20	66693.0
21	81255.0
22	95817.0
23	112881.5
24	129946.0
25	145941.5
26	177567.5
27	193198.0
28	206302.5
29	219407.0
30	230487.0
31	241567.0
32	249056.5
33	256546.0
34	263448.0
35	270350.0
36	275692.0
37	281034.0
38	282962.0
39	284890.0
40	284760.0
41	284630.0
42	283819.0
43	283008.0
44	280259.0
45	277510.0
46	272330.5
47	267151.0
48	261553.0
49	255955.0
50	256686.5
51	257418.0
52	341102.5
53	424787.0
54	316087.0
55	207387.0
56	194095.0
57	180803.0
58	168690.5
59	156578.0
60	145117.5
61	133657.0
62	123084.0
63	112511.0
64	102710.5
65	92910.0
66	84189.5
67	75469.0
68	67202.5
69	58936.0
70	51837.5
71	44739.0
72	38043.5
73	31348.0
74	26258.5
75	17028.5
76	12888.0
77	9969.5
78	7051.0
79	5384.5
80	3718.0
81	2709.0
82	1700.0
83	1208.5
84	717.0
85	514.5
86	312.0
87	222.0
88	132.0
89	89.0
90	46.0
91	36.0
92	26.0
93	16.5
94	7.0
95	8.5
96	10.0
97	5.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04345436699072129
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0014399727316556131
20	0.0
21	0.013433669661141607
22	3.645500586469907E-5
23	0.0
24	9.842851583468748E-4
25	0.0
26	0.09334304251656197
27	0.11443226340929037
28	0.12516826263644426
29	0.6982227273265812
30	0.13922166739728575
31	0.03483275810371996
32	0.123746517407721
33	0.016350070130317532
34	0.012795707058509373
35	0.018209275429417186
36	0.03091384497326481
37	0.041613389194553985
38	0.008749201407527777
39	0.01678753020069392
40	0.026557471772433273
41	0.013615944690465101
42	0.0175895403297173
43	0.0214902259572401
44	0.015894382557008795
45	0.024825858993860063
46	0.01913887807896701
47	0.00936893650722766
48	0.03295532530168796
49	0.011939014420688945
50	0.014582002345879627
51	0.011501554350312556
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5486215.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.69516162788486
#Duplication Level	Relative count
1	100.0
2	30.48996812698323
3	6.858705715234737
4	1.6374674025964848
5	0.622619241125379
6	0.3646668881052163
7	0.2614859468971512
8	0.1886939130311875
9	0.16254531834147237
10++	0.7576025272263408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	196508	3.581850146230142	TruSeq Adapter, Index 6 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1969210	4.324124	4.663838	2
AAAAA	1946790	4.1334367	4.292755	24
CGCCA	528315	3.1442087	61.943253	33
ATTTT	1388275	3.0280204	3.2255862	3
ACGCC	426640	2.5391011	61.5065	32
TCCAG	524930	2.4477513	49.24925	25
GGAAG	495370	2.4261787	53.094627	5
CACGC	398990	2.374545	61.732967	31
CGGAA	495180	2.3584745	51.62496	4
GAAGA	636215	2.3582869	40.690136	6
CTGAA	649565	2.3572907	39.27357	19
GAGCA	483810	2.3043208	51.4785	9
GCCAA	481485	2.2301078	47.946358	34
GATCG	461545	2.2131195	51.433792	1
CTCCA	468325	2.1236758	47.448715	24
ATCGG	439480	2.1073174	51.52446	2
CCAGT	443905	2.0699313	48.743286	26
TCTCG	437050	2.051728	48.94464	41
ATGCC	436105	2.0335598	48.718445	47
AGCAC	425135	1.9691098	49.651264	10
GTCTG	407000	1.9647534	51.08636	17
AGAGC	407925	1.9428909	51.072655	8
GCACA	418185	1.9369193	49.622997	11
TCGGA	400385	1.9198558	51.4648	3
TCTGA	523335	1.9120232	39.10732	18
TCACG	400465	1.8673702	48.65694	30
TGAAC	513295	1.8627628	38.681454	20
GTCAC	396355	1.8482053	48.770798	29
CACAC	406475	1.8308467	47.904663	12
CGTCT	388250	1.8226367	49.500305	16
AAGAG	483190	1.7910624	40.054085	7
ACGTC	381775	1.7802187	49.26899	15
CTCGT	374875	1.7598479	48.53626	42
CACGT	373185	1.7401636	49.280533	14
CAGTC	370215	1.7263144	48.572384	27
CCAAT	486395	1.716543	36.67847	35
GAACT	465915	1.6908194	38.49316	21
ATCTC	473110	1.6809332	37.11656	40
ACTCC	364005	1.6506244	47.12669	23
ACACG	356235	1.6499835	49.04458	13
AATAT	758240	1.6317153	23.169407	37
AACTC	454480	1.6039112	37.18652	22
AGTCA	413875	1.5019647	38.035286	28
CAATA	525585	1.4435545	28.708775	36
TATGC	382935	1.3990667	37.922802	46
ATATC	494545	1.3674732	28.90554	38
GTATG	363500	1.3656604	38.95145	45
TCGTA	362875	1.3257766	37.862198	43
CGTAT	354765	1.2961464	37.79142	44
TATCT	440405	1.2259933	28.975912	39
>>END_MODULE