FastQCFastQC Report
Wed 23 Jul 2014
LW186_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW186_ACAGTG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10193698
Filtered Sequences 0
Sequence length 51
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC 272345 2.671699710939053 TruSeq Adapter, Index 5 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 4539640 3.8841975 4.333546 2
AAAAA 4514465 3.8146636 4.040089 3
TTTTC 2417320 3.0720782 3.6177962 2
GAAAA 2322915 3.007602 3.1773283 2
CACAC 1023415 2.855055 41.370777 12
GGAAG 806950 2.4530573 46.027073 5
CGGAA 811635 2.3976681 44.61785 4
GAGCA 809280 2.390711 44.306488 9
TCCAG 808325 2.3263094 42.18255 25
CTCCA 831520 2.325527 41.059074 24
GAAGA 1162570 2.3064451 30.617249 6
CTGAA 1150665 2.2239559 29.34686 19
TCTCG 729110 2.1035872 42.058266 41
TCGGA 686655 2.0335405 44.313835 3
CAGTG 669465 1.9826323 42.587917 35
AGAGC 661450 1.9540035 43.873768 8
GCACA 680505 1.9535602 42.640297 11
ATCGG 654675 1.9388312 44.119873 2
AGCAC 662990 1.9032791 42.64232 10
CACAG 662205 1.9010257 41.4759 33
CCAGT 647155 1.862472 41.637596 26
GATCG 617085 1.827508 43.810925 1
TCTGA 940075 1.8214856 29.070969 18
GTCTG 609535 1.8096681 43.487305 17
CGTCT 617695 1.7821387 42.199253 16
GTCAC 618745 1.7807099 41.534164 29
CTCGT 615745 1.7765127 41.692345 42
ATGCC 606640 1.7458724 41.613075 47
CACGT 601490 1.7310511 42.309284 14
AAGAG 863945 1.7139972 29.993196 7
TGAAC 866825 1.675362 28.79466 20
ACGTC 576900 1.6602826 42.120213 15
ACTCC 591600 1.6545384 40.62506 23
ACACG 574810 1.6501364 42.22114 13
CAGTC 565910 1.628654 41.298084 27
TCACA 858865 1.61313 27.50519 30
AGTGA 803970 1.5990052 28.912682 36
ACACA 848445 1.5895792 27.461912 32
TGATC 783275 1.5176705 28.10063 38
GAACT 775275 1.4984182 28.57867 21
AACTC 794980 1.4931405 27.747595 22
ATCTC 790555 1.4885468 27.470272 40
ACAGT 764360 1.4773221 28.028334 34
GTGAT 739935 1.4753315 28.906893 37
AGTCA 704360 1.3613567 28.003042 28
GATCT 701720 1.3596498 28.035345 39
TCGTA 593170 1.1493238 27.993702 43
TATGC 589700 1.1426003 27.95089 46
GTATG 572350 1.1411893 28.771114 45
CGTAT 555215 1.0757823 27.873537 44
Produced by FastQC (version 0.10.1)