##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW186_ACAGTG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10193698	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81573242605382	34.0	31.0	34.0	31.0	34.0
2	32.99365372605702	34.0	33.0	34.0	31.0	34.0
3	33.0568129446252	34.0	33.0	34.0	31.0	34.0
4	36.39445488771592	37.0	37.0	37.0	35.0	37.0
5	36.342054767563255	37.0	37.0	37.0	35.0	37.0
6	36.34071835363378	37.0	37.0	37.0	35.0	37.0
7	36.3103254579447	37.0	37.0	37.0	35.0	37.0
8	36.302826707245984	37.0	37.0	37.0	35.0	37.0
9	38.10763336327994	39.0	39.0	39.0	37.0	39.0
10	38.05852145119465	39.0	38.0	39.0	37.0	39.0
11	38.064686338559376	39.0	38.0	39.0	37.0	39.0
12	38.04431738118983	39.0	38.0	39.0	35.0	39.0
13	38.03395833386471	39.0	38.0	39.0	35.0	39.0
14	39.57415208886903	41.0	40.0	41.0	37.0	41.0
15	39.53457852096462	41.0	39.0	41.0	37.0	41.0
16	39.505974573702304	41.0	39.0	41.0	37.0	41.0
17	39.48538803091871	41.0	39.0	41.0	37.0	41.0
18	39.41375857907503	41.0	39.0	41.0	37.0	41.0
19	39.47977623037292	41.0	39.0	41.0	37.0	41.0
20	39.46566545330262	41.0	39.0	41.0	37.0	41.0
21	39.43672276734115	41.0	39.0	41.0	37.0	41.0
22	39.33285839937577	40.0	39.0	41.0	36.0	41.0
23	39.29092749265281	40.0	39.0	41.0	36.0	41.0
24	39.23833548924051	40.0	39.0	41.0	36.0	41.0
25	39.22313668700014	40.0	39.0	41.0	36.0	41.0
26	39.10880261510592	40.0	39.0	41.0	36.0	41.0
27	39.018688507350326	40.0	39.0	41.0	36.0	41.0
28	38.87685107014157	40.0	38.0	41.0	35.0	41.0
29	38.62000306463857	40.0	38.0	41.0	35.0	41.0
30	38.608879525369495	40.0	38.0	41.0	35.0	41.0
31	38.84526802736357	40.0	38.0	41.0	35.0	41.0
32	38.83249552811943	40.0	38.0	41.0	35.0	41.0
33	38.86231650182299	40.0	38.0	41.0	35.0	41.0
34	38.99850289855556	40.0	39.0	41.0	36.0	41.0
35	39.06296586381115	40.0	39.0	41.0	36.0	41.0
36	39.037317958605406	40.0	39.0	41.0	35.0	41.0
37	38.9677458563124	40.0	39.0	41.0	35.0	41.0
38	38.84026297424153	40.0	38.0	41.0	35.0	41.0
39	38.850714137303264	40.0	38.0	41.0	35.0	41.0
40	38.76964552020278	40.0	38.0	41.0	35.0	41.0
41	38.78244283870289	40.0	38.0	41.0	35.0	41.0
42	38.748825597933156	40.0	38.0	41.0	35.0	41.0
43	38.69717191935645	40.0	38.0	41.0	35.0	41.0
44	38.636015212536215	40.0	38.0	41.0	35.0	41.0
45	38.54693811804117	40.0	38.0	41.0	34.0	41.0
46	38.466183322284024	40.0	38.0	41.0	34.0	41.0
47	38.38157065276997	40.0	38.0	41.0	34.0	41.0
48	38.32455807499888	40.0	38.0	41.0	34.0	41.0
49	38.2656684551573	40.0	38.0	41.0	34.0	41.0
50	38.155913094541354	40.0	37.0	41.0	34.0	41.0
51	37.18360579252005	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	11.0
11	7.0
12	8.0
13	11.0
14	8.0
15	23.0
16	57.0
17	137.0
18	362.0
19	717.0
20	1449.0
21	2459.0
22	4112.0
23	6634.0
24	10106.0
25	15197.0
26	21688.0
27	30619.0
28	42461.0
29	57242.0
30	75396.0
31	98429.0
32	127179.0
33	167855.0
34	226247.0
35	324671.0
36	503025.0
37	878100.0
38	1945413.0
39	5650252.0
40	3819.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.76981781372942	25.923243358043706	30.702058111879175	22.60488071634769
2	18.96592384824428	31.612276526143894	29.27645099943122	20.145348626180606
3	19.370791640089788	29.325873691765246	32.51144972119048	18.791884946954482
4	18.530615680393904	28.469177721372557	30.376287388541428	22.623919209692104
5	21.70060364746925	29.018487697006524	31.020803245299206	18.260105410225023
6	21.91769856238629	29.579128202542393	29.207898841029035	19.29527439404228
7	19.382838298721424	32.276314248273785	29.942244708446335	18.398602744558453
8	18.856091283065282	32.80020655899361	29.456974299218984	18.886727858722125
9	21.6794042750727	29.2061919040568	29.861086722404373	19.25331709846613
10	18.87986087090279	32.340461724488996	29.285858772743712	19.49381863186451
11	21.859888334930073	29.26792612455264	29.310462209102134	19.561723331415156
12	18.938397037071336	29.32469649385336	29.225615669602927	22.51129079947238
13	18.816056744078548	32.46855066728483	29.220308469016835	19.495084119619786
14	18.78322273231952	29.56468790815659	29.295021296491225	22.35706806303267
15	18.703928642971373	32.553613026401216	29.48885674266591	19.2536015879615
16	18.73556583685332	29.49575316043304	29.49590031017203	22.27278069254161
17	21.779485717548233	29.443515003093086	29.499235704255707	19.27776357510297
18	18.876692246523294	29.310540688962927	32.482765332070855	19.330001732442927
19	18.880107064011597	29.217695777163705	29.44101394531903	22.461183213505667
20	18.89931406639671	29.284524615110236	32.37344288598701	19.442718432506044
21	21.944233638088193	29.26832833857174	29.420746648587738	19.366691374752325
22	18.915969029480383	32.349401502536764	29.361936899420364	19.372692568562485
23	18.921769116565944	32.406129748007054	29.374452725595756	19.29764840983125
24	18.94946303004036	29.322985127806334	29.455234943303076	22.272316898850228
25	18.87277806346627	29.337557381040718	32.39912542043133	19.390539135061683
26	18.893866682796002	29.387063003186658	29.424080613160868	22.294989700856473
27	18.94731890200432	29.40248491650047	29.41145158592687	22.238744595568345
28	18.945979354710705	32.387841487317324	29.33143699326639	19.33474216470558
29	21.952743617497596	29.4458486614953	29.32553518650466	19.27587253450244
30	19.10324745039372	29.324924710519724	32.22917275926061	19.342655079825953
31	19.014228805121483	29.321893816533457	29.299715588762272	22.364161789582788
32	18.997067172735463	32.322940830438306	29.258829299120194	19.42116269770604
33	19.02537254666099	29.39837887834268	29.346907534763023	22.2293410402333
34	18.993928549318383	32.36307987367521	29.33051373187054	19.31247784513587
35	18.974548162553063	29.546521559321803	29.336984564847217	22.14194571327792
36	19.063630513333152	32.389029748354716	29.35181334510364	19.195526393208496
37	21.95105722356117	29.443284112626838	29.355351439220367	19.25030722459163
38	19.057747065172272	29.410418894740598	32.25889685188054	19.272937188206594
39	21.95604201745066	29.476108390744905	29.384890244367888	19.182959347436544
40	19.05888766928971	32.38864005184202	29.30540806648087	19.247064212387407
41	19.007504535991586	29.487443007606966	32.216818288270375	19.28823416813108
42	19.122332993718967	29.371939742259386	29.31971225924141	22.186015004780245
43	19.017957164630122	29.334905619185186	32.258352547816415	19.388784668368274
44	19.057908540633818	29.373114775587222	29.347889328674427	22.221087355104533
45	21.98501461055846	29.430090096808442	29.395845067243894	19.189050225389206
46	19.03432023354592	29.51625644406457	32.26110587757168	19.18831744481783
47	19.02325629765267	32.48096738412321	29.3650418345717	19.130734483652425
48	19.170536559880414	29.435737627667713	32.234749130531334	19.158976681920535
49	22.02565944792952	29.350916060559413	29.305627871779876	19.31779661973119
50	19.11468313420989	29.356555081008263	29.227603150261427	22.30115863452042
51	19.15955528433429	29.309360338599255	29.282654637000643	22.248429740065813
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.37469853007712
2	39.11127247442489
3	38.16267658704427
4	41.15453489008601
5	39.960709057694274
6	41.212972956428565
7	37.78144104327988
8	37.7428191417874
9	40.932721373538826
10	38.3736795027673
11	41.42161166634523
12	41.44968783654372
13	38.311140863698334
14	41.140290795352186
15	37.95753023093288
16	41.00834652939492
17	41.05724929265121
18	38.20669397896622
19	41.34129027751726
20	38.34203249890275
21	41.31092501284052
22	38.288661598042864
23	38.219417526397194
24	41.221779928890584
25	38.26331719852795
26	41.18885638365247
27	41.18606349757267
28	38.28072151941629
29	41.22861615200004
30	38.445902530219676
31	41.37839059470427
32	38.41822987044151
33	41.25471358689429
34	38.30640639445425
35	41.11649387583098
36	38.25915690654165
37	41.201364448152795
38	38.33068425337886
39	41.13900136488721
40	38.30595188167712
41	38.29573870412266
42	41.30834799849921
43	38.4067418329984
44	41.278995895738355
45	41.17406483594767
46	38.22263767836375
47	38.15399078130509
48	38.32951324180095
49	41.34345606766071
50	41.41584176873031
51	41.4079850244001
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	51.5
2	51.0
3	144.0
4	237.0
5	471.0
6	705.0
7	1425.5
8	2146.0
9	4008.0
10	5870.0
11	9960.0
12	14050.0
13	21341.5
14	28633.0
15	41221.0
16	53809.0
17	73143.5
18	92478.0
19	120026.5
20	147575.0
21	181872.0
22	216169.0
23	255829.0
24	295489.0
25	335413.0
26	412939.5
27	450542.0
28	483346.5
29	516151.0
30	544630.5
31	573110.0
32	591148.5
33	609187.0
34	627356.5
35	645526.0
36	656059.5
37	666593.0
38	668137.0
39	669681.0
40	663721.0
41	657761.0
42	644617.0
43	631473.0
44	609377.5
45	587282.0
46	550372.5
47	513463.0
48	477917.5
49	442372.0
50	419533.5
51	396695.0
52	489028.0
53	581361.0
54	413353.5
55	245346.0
56	215229.0
57	185112.0
58	166189.0
59	147266.0
60	131033.0
61	114800.0
62	101920.5
63	89041.0
64	78350.0
65	67659.0
66	59990.0
67	52321.0
68	45860.5
69	39400.0
70	34177.0
71	28954.0
72	24482.0
73	20010.0
74	16523.0
75	10540.5
76	8045.0
77	6272.0
78	4499.0
79	3455.5
80	2412.0
81	1775.0
82	1138.0
83	821.0
84	504.0
85	349.5
86	195.0
87	141.0
88	87.0
89	65.5
90	44.0
91	28.5
92	13.0
93	11.5
94	10.0
95	6.5
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04371328246137957
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0016676970418389872
20	0.0
21	0.013272906456518529
22	3.923993039621146E-5
23	0.0
24	0.001088908068494868
25	0.0
26	0.09178219719673861
27	0.11325624910606534
28	0.1244200093037875
29	0.70441561050759
30	0.13723184657815055
31	0.0345703786790623
32	0.12494974836413636
33	0.01588236182786659
34	0.012174188405424606
35	0.016706400366187032
36	0.030253986335479037
37	0.0400639689345319
38	0.008348295191793989
39	0.016157041340640072
40	0.026840112391008638
41	0.013037466874141259
42	0.017128229617946304
43	0.021258232292147558
44	0.015784262001876062
45	0.023916737576490887
46	0.019178515981148354
47	0.00914290378231727
48	0.03216693294229435
49	0.011722929205868175
50	0.01498965341135278
51	0.010967560545741103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.0193698E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.77081888914567
#Duplication Level	Relative count
1	100.0
2	19.0991244687932
3	3.297322205724233
4	0.9446520915467718
5	0.549127028825969
6	0.42624545594183605
7	0.367364702268189
8	0.33600430085505095
9	0.3091239567866469
10++	2.672674210229891
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC	272345	2.671699710939053	TruSeq Adapter, Index 5 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	4539640	3.8841975	4.333546	2
AAAAA	4514465	3.8146636	4.040089	3
TTTTC	2417320	3.0720782	3.6177962	2
GAAAA	2322915	3.007602	3.1773283	2
CACAC	1023415	2.855055	41.370777	12
GGAAG	806950	2.4530573	46.027073	5
CGGAA	811635	2.3976681	44.61785	4
GAGCA	809280	2.390711	44.306488	9
TCCAG	808325	2.3263094	42.18255	25
CTCCA	831520	2.325527	41.059074	24
GAAGA	1162570	2.3064451	30.617249	6
CTGAA	1150665	2.2239559	29.34686	19
TCTCG	729110	2.1035872	42.058266	41
TCGGA	686655	2.0335405	44.313835	3
CAGTG	669465	1.9826323	42.587917	35
AGAGC	661450	1.9540035	43.873768	8
GCACA	680505	1.9535602	42.640297	11
ATCGG	654675	1.9388312	44.119873	2
AGCAC	662990	1.9032791	42.64232	10
CACAG	662205	1.9010257	41.4759	33
CCAGT	647155	1.862472	41.637596	26
GATCG	617085	1.827508	43.810925	1
TCTGA	940075	1.8214856	29.070969	18
GTCTG	609535	1.8096681	43.487305	17
CGTCT	617695	1.7821387	42.199253	16
GTCAC	618745	1.7807099	41.534164	29
CTCGT	615745	1.7765127	41.692345	42
ATGCC	606640	1.7458724	41.613075	47
CACGT	601490	1.7310511	42.309284	14
AAGAG	863945	1.7139972	29.993196	7
TGAAC	866825	1.675362	28.79466	20
ACGTC	576900	1.6602826	42.120213	15
ACTCC	591600	1.6545384	40.62506	23
ACACG	574810	1.6501364	42.22114	13
CAGTC	565910	1.628654	41.298084	27
TCACA	858865	1.61313	27.50519	30
AGTGA	803970	1.5990052	28.912682	36
ACACA	848445	1.5895792	27.461912	32
TGATC	783275	1.5176705	28.10063	38
GAACT	775275	1.4984182	28.57867	21
AACTC	794980	1.4931405	27.747595	22
ATCTC	790555	1.4885468	27.470272	40
ACAGT	764360	1.4773221	28.028334	34
GTGAT	739935	1.4753315	28.906893	37
AGTCA	704360	1.3613567	28.003042	28
GATCT	701720	1.3596498	28.035345	39
TCGTA	593170	1.1493238	27.993702	43
TATGC	589700	1.1426003	27.95089	46
GTATG	572350	1.1411893	28.771114	45
CGTAT	555215	1.0757823	27.873537	44
>>END_MODULE