FastQCFastQC Report
Wed 23 Jul 2014
LW185_TGACCA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW185_TGACCA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11701716
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC 515057 4.401551020380259 TruSeq Adapter, Index 4 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 4354955 4.378118 4.8787317 2
AAAAA 4329750 4.1300645 4.3429365 3
ATTTT 3034805 3.019066 3.2243395 3
GGAAG 1157490 2.734701 67.2688 5
GAGCA 1161825 2.6354713 64.34748 9
CGGAA 1159220 2.6295621 64.47049 4
GAAGA 1497275 2.614536 50.250942 6
TCCAG 1175355 2.5868635 61.040836 25
GATCG 1116435 2.5592513 64.76696 1
CTGAA 1463180 2.479007 47.941856 19
TCTCG 1088665 2.421368 61.21956 41
ATCGG 1054230 2.4166563 64.80741 2
CTCCA 1135850 2.4002168 58.338295 24
TCGGA 1007350 2.3091915 64.82679 3
AGAGC 987350 2.239694 64.067924 8
CACTG 1014935 2.2337923 60.65457 31
CCAGT 1010545 2.2241302 60.532 26
GCACA 1020365 2.2222767 61.290928 11
AGCAC 1015320 2.211289 61.36352 10
ATGCC 1003050 2.207634 60.233738 47
GTCTG 950340 2.201509 64.386086 17
CTGAC 982270 2.1618989 60.228035 33
CACAC 1031915 2.1578014 58.497074 12
GACCA 980345 2.135116 58.92142 35
CGTCT 954095 2.122062 61.596962 16
CTCGT 946845 2.1059368 60.629433 42
TGACC 949380 2.0895107 59.605076 34
TCTGA 1219345 2.0877023 48.046944 18
GTCAC 946575 2.083337 60.688576 29
AAGAG 1189315 2.0767775 49.711494 7
ACGTC 933245 2.053999 61.01377 15
TGAAC 1195605 2.0256653 47.46556 20
CACGT 919510 2.0237694 61.258736 14
CAGTC 892035 1.9632989 60.315018 27
ACACG 895420 1.950156 60.662075 13
ACCAA 1188080 1.912444 43.70382 36
CCAAT 1165905 1.8965667 44.152287 37
ACTCC 894700 1.8906317 58.11729 23
ATCTC 1143145 1.8791795 45.24989 40
ACTGA 1107400 1.8762232 46.879757 32
GAACT 1099415 1.8626945 47.282185 21
CAATC 1129760 1.83777 44.149437 38
TCACT 1106160 1.8183812 45.46879 30
AACTC 1098850 1.787489 45.30146 22
AGTCA 995005 1.6857969 46.73383 28
AATCT 1277035 1.6160144 34.825565 39
GTATG 875830 1.5618409 48.529034 45
TATGC 902725 1.545601 46.573563 46
TCGTA 888470 1.5211943 46.622417 43
CGTAT 870450 1.4903413 46.56971 44
Produced by FastQC (version 0.10.1)